PCBP2 poly(rC) binding protein 2 [Homo sapiens] - Gene

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Summary

Official Symbol: PCBP2provided by HGNC
Official Full Name: poly(rC) binding protein 2provided by HGNC
Primary source: HGNC:8648
See related: Ensembl:ENSG00000197111; HPRD:03129; MIM:601210; Vega:OTTHUMG00000169439
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: HNRPE2; hnRNP-E2; MGC110998
Summary: The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. Thsi gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

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Genomic context

Location :: 12q13.12-q13.13

Sequence :: Chromosome: 12; NC_000012.11 (53845886..53874946)

See PCBP2 in Epigenomics, MapViewer

Chromosome 12 - NC_000012.11 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PCBP2 stabilizes the mRNA of STAT1 and STAT2 through binding the 3'Untranslated Region (UTR) of these two molecules.
Title: PCBP2 enhances the antiviral activity of IFN-α against HCV by stabilizing the mRNA of STAT1 and STAT2.
SRp75 inhibits splicing of tau exon 10 by binding to the CA repeat in the intronic splicing silencer downstream of the exon, and requires hnRNPG to exert its full regulatory effect on tau exon 10.
Title: An SRp75/hnRNPG complex interacting with hnRNPE2 regulates the 5' splice site of tau exon 10, whose misregulation causes frontotemporal dementia.
Overall, the results presented here suggest that cellular PCBP2 and PCBP1 antagonize vesiculovirus growth by affecting viral gene expression and highlight the importance of these two cellular proteins in restricting virus infections.
Title: Antagonistic effects of cellular poly(C) binding proteins on vesicular stomatitis virus gene expression.
study demonstrates the mechanism of the participation of PCBP2 in hepatitis C virus translation and replication
Title: Poly(C)-binding protein 2 interacts with sequences required for viral replication in the hepatitis C virus (HCV) 5' untranslated region and directs HCV RNA replication through circularizing the viral genome.
Study reports that loss of miR-328 occurs in blast crisis chronic myelogenous leukemia (CML-BC) in a BCR/ABL dose- and kinase-dependent manner through the MAPK-hnRNP E2 pathway.
Title: miR-328 functions as an RNA decoy to modulate hnRNP E2 regulation of mRNA translation in leukemic blasts.
The authors established that the 5'CL-PCBP complex is required for optimal poliovirus viral RNA translation and identified the KH3 domain of PCBP2 as the functional region.
Title: The 5'CL-PCBP RNP complex, 3' poly(A) tail and 2A(pro) are required for optimal translation of poliovirus RNA.
PCBP2-AIP4 axis defines a new signaling cascade for MAVS degradation and 'fine tuning' of antiviral innate immunity.
Title: PCBP2 mediates degradation of the adaptor MAVS via the HECT ubiquitin ligase AIP4.
Results establish the compositional diversity among P-bodies, and that PCBP2, probably in complex with other mRNP factors, may dynamically recognize such differences and accumulate to specific P-bodies.
Title: Selective localization of PCBP2 to cytoplasmic processing bodies.
reveal a pivotal role for alphaCP1 and alphaCP2 in a p53-independent pathway of p21(WAF) control and cell cycle progression
Title: Depletion of the poly(C)-binding proteins alphaCP1 and alphaCP2 from K562 cells leads to p53-independent induction of cyclin-dependent kinase inhibitor (CDKN1A) and G1 arrest.
analysis of the human poly(C)-binding protein containing the first and second KH domains reveals insights into its regulatory mechanisms
Title: Structure of a construct of a human poly(C)-binding protein containing the first and second KH domains reveals insights into its regulatory mechanisms.

Submit: New GeneRIF Correction See all (27)

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Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

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Products	Interactant	Other Gene	Source	Pubs	Description
CAA55015.1	AAK67712.1		BIND	PubMed	DEN core interacts with hnRNP E2. This interaction was modelled on a demonstrated interaction between DEN core from Dengue virus type 2 (strain Taiwan PL046) and hnRNP E2 from an unspecified species.
Q15366	P62633	CNBP	HPRD	PubMed
Q15366	P16989	CSDA	HPRD	PubMed
Q15366	P21333	FLNA	HPRD	PubMed
Q15366	Q14103	HNRNPD	HPRD	PubMed
Q15366	P61978	HNRNPK	HPRD	PubMed
Q15366	P14866	HNRNPL	HPRD	PubMed
Q15366	P11940	PABPC1	HPRD	PubMed
Q15366	Q15365	PCBP1	HPRD	PubMed
Q15366	Q15366	PCBP2	HPRD	PubMed
Q15366	O75400	PRPF40A	HPRD	PubMed
Q15366	P26599	PTBP1	HPRD	PubMed
Q15366	Q96PU8	QKI	HPRD	PubMed
Q15366	P84103	SRSF3	HPRD	PubMed
Q15366	Q16629	SRSF7	HPRD	PubMed
Q15366	P67809	YBX1	HPRD	PubMed
BioGRID:111127	BioGRID:224458	Aif1l	BioGRID	PubMed	Affinity Capture-MS
BioGRID:111127	BioGRID:107459	CDK9	BioGRID	PubMed	Affinity Capture-MS
BioGRID:111127	BioGRID:971788	EBNA-LP	BioGRID	PubMed	Affinity Capture-MS
BioGRID:111127	BioGRID:109268	H2AFX	BioGRID	PubMed	Affinity Capture-MS
BioGRID:111127	BioGRID:115331	HDAC5	BioGRID	PubMed	Affinity Capture-MS
BioGRID:111127	BioGRID:109425	HNRNPD	BioGRID	PubMed	Two-hybrid
BioGRID:111127	BioGRID:109431	HNRNPK	BioGRID	PubMed	Reconstituted Complex; Two-hybrid
BioGRID:111127	BioGRID:109432	HNRNPL	BioGRID	PubMed	Reconstituted Complex; Two-hybrid
BioGRID:111127	BioGRID:108918	KAT2A	BioGRID	PubMed	Affinity Capture-MS
BioGRID:111127	BioGRID:121122	MEPCE	BioGRID	PubMed	Affinity Capture-MS
BioGRID:111127	BioGRID:117939	PABPC1	BioGRID	PubMed	Reconstituted Complex; Two-hybrid
BioGRID:111127	BioGRID:111127	PCBP2	BioGRID	PubMed	Reconstituted Complex; Two-hybrid
BioGRID:111127	BioGRID:128147	POLR3H	BioGRID	PubMed	Affinity Capture-MS
BioGRID:111127	BioGRID:111657	PSMA3	BioGRID	PubMed	Affinity Capture-MS
BioGRID:111127	BioGRID:111697	PTBP1	BioGRID	PubMed	Reconstituted Complex; Two-hybrid
BioGRID:111127	BioGRID:114166	RUVBL1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:111127	BioGRID:116067	RUVBL2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:111127	BioGRID:112497	SUMO2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:111127	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS

Function	Evidence Code	Pubs
DNA binding	IEA Inferred from Electronic Annotation more info
RNA binding	IDA Inferred from Direct Assay more info	PubMed
RNA binding	IEA Inferred from Electronic Annotation more info
enzyme binding	IPI Inferred from Physical Interaction more info	PubMed
protein binding	IPI Inferred from Physical Interaction more info	PubMed
ubiquitin protein ligase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
RNA splicing	TAS Traceable Author Statement more info
gene expression	TAS Traceable Author Statement more info
innate immune response	TAS Traceable Author Statement more info
mRNA metabolic process	NAS Non-traceable Author Statement more info	PubMed
negative regulation of defense response to virus	IMP Inferred from Mutant Phenotype more info	PubMed
negative regulation of type I interferon production	TAS Traceable Author Statement more info
nuclear mRNA splicing, via spliceosome	TAS Traceable Author Statement more info
proteasomal ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
response to virus	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
cytoplasm	IDA Inferred from Direct Assay more info	PubMed
cytoplasm	NAS Non-traceable Author Statement more info
cytosol	TAS Traceable Author Statement more info
NOT nucleolus	IDA Inferred from Direct Assay more info	PubMed
nucleoplasm	TAS Traceable Author Statement more info
nucleus	IDA Inferred from Direct Assay more info	PubMed
nucleus	NAS Non-traceable Author Statement more info
ribonucleoprotein complex	IEA Inferred from Electronic Annotation more info

General protein information

Preferred Names: poly(rC)-binding protein 2

Names: poly(rC)-binding protein 2; hnRNP E2; alpha-CP2; heterogenous nuclear ribonucleoprotein E2; heterogeneous nuclear ribonucleoprotein E2

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NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001098620.2 → NP_001092090.1 poly(rC)-binding protein 2 isoform c

Status: REVIEWED

Description

Transcript Variant: This variant (3) uses an alternate in-frame splice site and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (c) has the same N- and C-termini but is shorter compared to isoform a.

Source sequence(s)

AB188306, AC023509, AK130583, BF509278

Consensus CDS

CCDS44903.1

Related

ENSP00000352228, OTTHUMP00000242722, ENST00000359282, OTTHUMT00000407073

Conserved Domains (2) summary

cd00105
Location:255 – 317
Blast Score: 159

KH-I; K homology RNA-binding domain, type I. KH binds single-stranded RNA or DNA. It is found in a wide variety of proteins including ribosomal proteins, transcription factors and post-transcriptional modifiers of mRNA. There are two different KH domains that ...

cd02396
Location:99 – 163
Blast Score: 195

PCBP_like_KH; K homology RNA-binding domain, PCBP_like. Members of this group possess KH domains in a tandem arrangement. Most members, similar to the poly(C) binding proteins (PCBPs) and Nova, containing three KH domains, with the first and second domains, which are ...
NM_001128911.1 → NP_001122383.1 poly(rC)-binding protein 2 isoform d

Status: REVIEWED

Description

Transcript Variant: This variant (4) uses an alternate in-frame splice site compared to variant 1. The resulting isoform (d) has the same N- and C-termini but is one amino acid shorter compared to isoform a.

Source sequence(s)

AB188306, AC023509, AK130583, BF509278, X78136

Consensus CDS

CCDS44901.1

UniProtKB/Swiss-Prot

Q15366

Related

ENSP00000408949, OTTHUMP00000243002, ENST00000439930, OTTHUMT00000407545

Conserved Domains (2) summary

cd00105
Location:289 – 351
Blast Score: 165

KH-I; K homology RNA-binding domain, type I. KH binds single-stranded RNA or DNA. It is found in a wide variety of proteins including ribosomal proteins, transcription factors and post-transcriptional modifiers of mRNA. There are two different KH domains that ...

cd02396
Location:99 – 163
Blast Score: 201

PCBP_like_KH; K homology RNA-binding domain, PCBP_like. Members of this group possess KH domains in a tandem arrangement. Most members, similar to the poly(C) binding proteins (PCBPs) and Nova, containing three KH domains, with the first and second domains, which are ...
NM_001128912.1 → NP_001122384.1 poly(rC)-binding protein 2 isoform e

Status: REVIEWED

Description

Transcript Variant: This variant (5) uses two alternate in-frame splice sites compared to variant 1. The resulting isoform (e) has the same N- and C-termini but is five amino acids shorter compared to isoform a.

Source sequence(s)

AB188306, AB208825, AC023509, AK130583, BF509278

Consensus CDS

CCDS55830.1

UniProtKB/TrEMBL

Q59HD4

Related

ENSP00000448927, OTTHUMP00000243006, ENST00000552296, OTTHUMT00000407550

Conserved Domains (2) summary

cd00105
Location:285 – 347
Blast Score: 164

KH-I; K homology RNA-binding domain, type I. KH binds single-stranded RNA or DNA. It is found in a wide variety of proteins including ribosomal proteins, transcription factors and post-transcriptional modifiers of mRNA. There are two different KH domains that ...

cd02396
Location:99 – 163
Blast Score: 200

PCBP_like_KH; K homology RNA-binding domain, PCBP_like. Members of this group possess KH domains in a tandem arrangement. Most members, similar to the poly(C) binding proteins (PCBPs) and Nova, containing three KH domains, with the first and second domains, which are ...
NM_001128913.1 → NP_001122385.1 poly(rC)-binding protein 2 isoform f

Status: REVIEWED

Description

Transcript Variant: This variant (6) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (f) has the same N- and C-termini but is shorter compared to isoform a.

Source sequence(s)

AB188306, AC023509, AK130583, BC107688, BF509278

Consensus CDS

CCDS44902.1

Related

ENSP00000394116, ENST00000447282

Conserved Domains (2) summary

cd00105
Location:259 – 321
Blast Score: 160

KH-I; K homology RNA-binding domain, type I. KH binds single-stranded RNA or DNA. It is found in a wide variety of proteins including ribosomal proteins, transcription factors and post-transcriptional modifiers of mRNA. There are two different KH domains that ...

cd02396
Location:99 – 163
Blast Score: 195

PCBP_like_KH; K homology RNA-binding domain, PCBP_like. Members of this group possess KH domains in a tandem arrangement. Most members, similar to the poly(C) binding proteins (PCBPs) and Nova, containing three KH domains, with the first and second domains, which are ...
NM_001128914.1 → NP_001122386.1 poly(rC)-binding protein 2 isoform g

Status: REVIEWED

Description

Transcript Variant: This variant (7) uses an alternate in-frame splice site and lacks two alternate in-frame exons compared to variant 1. The resulting isoform (g) has the same N- and C-termini but is shorter compared to isoform a.

Source sequence(s)

AB188306, AC023509, AK130583, AK225357, BF509278

Consensus CDS

CCDS44904.1

UniProtKB/TrEMBL

G3V0E8

Related

ENSP00000390304, ENST00000437231

Conserved Domains (2) summary

cd00105
Location:242 – 304
Blast Score: 159

KH-I; K homology RNA-binding domain, type I. KH binds single-stranded RNA or DNA. It is found in a wide variety of proteins including ribosomal proteins, transcription factors and post-transcriptional modifiers of mRNA. There are two different KH domains that ...

cd02396
Location:99 – 163
Blast Score: 194

PCBP_like_KH; K homology RNA-binding domain, PCBP_like. Members of this group possess KH domains in a tandem arrangement. Most members, similar to the poly(C) binding proteins (PCBPs) and Nova, containing three KH domains, with the first and second domains, which are ...
NM_005016.5 → NP_005007.2 poly(rC)-binding protein 2 isoform a

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).

Source sequence(s)

AB188306, AC023509, AK130583, BC035420, BF509278

Consensus CDS

CCDS44900.1

UniProtKB/Swiss-Prot

Q15366

Related

ENSP00000352438, OTTHUMP00000242723, ENST00000359462, OTTHUMT00000407074

Conserved Domains (2) summary

cd00105
Location:290 – 352
Blast Score: 165

KH-I; K homology RNA-binding domain, type I. KH binds single-stranded RNA or DNA. It is found in a wide variety of proteins including ribosomal proteins, transcription factors and post-transcriptional modifiers of mRNA. There are two different KH domains that ...

cd02396
Location:99 – 163
Blast Score: 201

PCBP_like_KH; K homology RNA-binding domain, PCBP_like. Members of this group possess KH domains in a tandem arrangement. Most members, similar to the poly(C) binding proteins (PCBPs) and Nova, containing three KH domains, with the first and second domains, which are ...
NM_031989.4 → NP_114366.1 poly(rC)-binding protein 2 isoform b

Status: REVIEWED

Description

Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.

Source sequence(s)

AB188306, AC023509, AK130583, AK292141, BF509278

Consensus CDS

CCDS8859.1

UniProtKB/Swiss-Prot

Q15366

Related

ENSP00000448762, OTTHUMP00000243320, ENST00000546463, OTTHUMT00000408111

Conserved Domains (2) summary

cd00105
Location:286 – 348
Blast Score: 165

KH-I; K homology RNA-binding domain, type I. KH binds single-stranded RNA or DNA. It is found in a wide variety of proteins including ribosomal proteins, transcription factors and post-transcriptional modifiers of mRNA. There are two different KH domains that ...

cd02396
Location:99 – 163
Blast Score: 200

PCBP_like_KH; K homology RNA-binding domain, PCBP_like. Members of this group possess KH domains in a tandem arrangement. Most members, similar to the poly(C) binding proteins (PCBPs) and Nova, containing three KH domains, with the first and second domains, which are ...

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000012.11 Reference GRCh37.p5 Primary Assembly

Range

53845886..53874946

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000144.1 Alternate HuRef

Range

50885765..50914832

Download

GenBank, FASTA, Sequence Viewer (Graphics)

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Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AC023509.42 (141911..170552)	None
genomic	AC068889.35	None
genomic	CH471054.1	EAW96706.1
		EAW96707.1
		EAW96708.1
		EAW96709.1
		EAW96710.1
		EAW96711.1
genomic	CS185528.1	CAJ42709.1
mRNA	AB188306.1	BAD36897.1
mRNA	AB208825.1	BAD92062.1
mRNA	AK023529.1	None
mRNA	AK123458.1	None
mRNA	AK130583.1	None
mRNA	AK225357.1	None
mRNA	AK292141.1	BAF84830.1
mRNA	AK299210.1	BAG61249.1
mRNA	AK302067.1	BAG63457.1
mRNA	BC001155.1	AAH01155.1
mRNA	BC035420.1	None
mRNA	BC071942.1	AAH71942.1
mRNA	BC107688.1	AAI07689.1
mRNA	BE748080.1	None
mRNA	BF509278.1	None
mRNA	BM764315.1	None
mRNA	BU844465.1	None
mRNA	CX164318.1	None
mRNA	X78136.1	CAA55015.1