NFIC nuclear factor I/C (CCAAT-binding transcription factor) [Homo sapiens] - Gene

Help top of page

Summary

Official Symbol: NFICprovided by HGNC
Official Full Name: nuclear factor I/C (CCAAT-binding transcription factor)provided by HGNC
Primary source: HGNC:7786
See related: Ensembl:ENSG00000141905; MIM:600729
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: CTF; NFI; CTF5; NF-I; MGC20153
Summary: The protein encoded by this gene belongs to the CTF/NF-I family. These are dimeric DNA-binding proteins, and function as cellular transcription factors and as replication factors for adenovirus DNA replication. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]

Help top of page

Genomic context

Location :: 19p13.3

Sequence :: Chromosome: 19; NC_000019.9 (3359616..3463603)

See NFIC in Epigenomics, MapViewer

Chromosome 19 - NC_000019.9 Genomic Context describing neighboring genes

Help top of page

Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

Help top of page

Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

novel role of NF1-C2 in tumor development and epithelial-to-mesenchymal transition in breast cancer; NF1-C2 is lost during tumor progression and virtully absent from lymph node metastases; FoxF1 was was found to be a direct repressed target of NF1-C2
Title: Nuclear Janus-activated kinase 2/nuclear factor 1-C2 suppresses tumorigenesis and epithelial-to-mesenchymal transition by repressing Forkhead box F1.
Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.
A specific region (-688 to -611 bp) was discovered in the IRS2 promoter essential for basal promoter activity and oxidative stress induced transcription depending on ERK activation and SP1 and NFI binding in human hepatocytes.
Title: Identification of a region in the human IRS2 promoter essential for stress induced transcription depending on SP1, NFI binding and ERK activation in HepG2 cells.
These results imply a central role of NFI-C in the interplay of the two signaling pathways and in regulation of the progression of tissue regeneration.
Title: Nuclear factor I-C links platelet-derived growth factor and transforming growth factor beta1 signaling to skin wound healing progression.
Knockdown of NFIB and NFIC expression using siRNA decreased and increased IGFBP5 expression, respectively.
Title: Nuclear factor I transcription factors regulate IGF binding protein 5 gene transcription in human osteoblasts.
These are the first studies to analyze the expression of NFI-C isoforms in dental versus nondental cells/tissues.
Title: Tissue- and cell-specific alternative splicing of NFIC.
These results suggest that alpha5 gene expression is likely dictated by subtle alterations in the nuclear ratio of TFs that either repress (NFI) or activate (Sp1 and AP-1) alpha5 transcription in corneal epithelial cells.
Title: Differential binding of the transcription factors Sp1, AP-1, and NFI to the promoter of the human alpha5 integrin gene dictates its transcriptional activity.
All NFI isoforms were found to be expressed in human corneal epithelial cells grown on laminin.
Title: Transcriptional regulation of the human alpha6 integrin gene by the transcription factor NFI during corneal wound healing.
we suggest that bHLH factors recruit NFI to enhance skeletal muscle Na(+) channel expression.
Title: Basic helix-loop-helix factors recruit nuclear factor I to enhance expression of the NaV 1.4 Na+ channel gene.
diminished NF-1C-dependent repression may be one mechanism underlying increased basal CYP17 promoter activity and altered gene expression in PCOS (polycystic ovary syndrome) theca cells.
Title: Increased cytochrome P450 17alpha-hydroxylase promoter function in theca cells isolated from patients with polycystic ovary syndrome involves nuclear factor-1.

Submit: New GeneRIF Correction See all (13)

Help top of page

Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

Hundreds of variants clustered in genomic loci and biological pathways affect human height.

Help top of page

HIV-1 protein interactions

Protein	Gene	Interaction	Pubs
Tat	tat	HIV-1 Tat synergizes with CTF to activate transcription and enhance transcript elongation and exon skipping	PubMed

Go to the HIV-1, Human Protein Interaction Database

Help top of page

Products	Interactant	Other Gene	Source	Pubs	Description
P08651	Q92793	CREBBP	HPRD	PubMed
P08651	P35754	GLRX	HPRD	PubMed
P08651	Q9BRT6	LLPH	HPRD	PubMed
P08651	O00712	NFIB	HPRD	PubMed
P08651	P22670	RFX1	HPRD	PubMed
P08651	Q9H0I9	TKTL2	HPRD	PubMed
P08651	P31314	TLX1	HPRD	PubMed
P08651	Tumor protein D52-like 3	TPD52L3	HPRD	PubMed
P08651	Q8WYQ9	ZCCHC14	HPRD	PubMed
P08651	Q9P0L1	ZNF167	HPRD	PubMed
BioGRID:110854	BioGRID:124024	LLPH	BioGRID	PubMed	Two-hybrid
BioGRID:110854	BioGRID:110853	NFIB	BioGRID	PubMed	Reconstituted Complex
BioGRID:110854	BioGRID:111921	RFX1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:110854	BioGRID:110263	SMAD3	BioGRID	PubMed	Affinity Capture-Western
BioGRID:110854	BioGRID:121411	SMURF1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:110854	BioGRID:112497	SUMO2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:110854	BioGRID:123871	TKTL2	BioGRID	PubMed	Two-hybrid
BioGRID:110854	BioGRID:124633	TPD52L3	BioGRID	PubMed	Two-hybrid
BioGRID:110854	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western
BioGRID:110854	BioGRID:116786	ZCCHC14	BioGRID	PubMed	Two-hybrid
BioGRID:110854	BioGRID:120978	ZNF167	BioGRID	PubMed	Two-hybrid

Function	Evidence Code	Pubs
DNA binding	IEA Inferred from Electronic Annotation more info
sequence-specific DNA binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
DNA replication	IEA Inferred from Electronic Annotation more info
negative regulation of transcription from RNA polymerase II promoter	IDA Inferred from Direct Assay more info	PubMed
negative regulation of transcription from RNA polymerase II promoter	IGI Inferred from Genetic Interaction more info	PubMed
positive regulation of transcription from RNA polymerase II promoter	IDA Inferred from Direct Assay more info	PubMed
regulation of transcription, DNA-dependent	TAS Traceable Author Statement more info	PubMed
transcription from RNA polymerase II promoter	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
intracellular	IEA Inferred from Electronic Annotation more info
nucleus	IEA Inferred from Electronic Annotation more info

General protein information

Preferred Names: nuclear factor 1 C-type

Names: nuclear factor 1 C-type; NF1-C; NF-I/C; TGGCA-binding protein; CCAAT-box-binding transcription factor

Help top of page

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_030333.1 RefSeqGene

Range

5001..114655

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001245002.1 → NP_001231931.1 nuclear factor 1 C-type isoform 1

Status: REVIEWED

Description

Transcript Variant: This variant (1) encodes the longest isoform (1).

Source sequence(s)

AC005551, AK289885, AK297825, BC012120, BF763988, BU727954, CB053896, CK431049

UniProtKB/TrEMBL

B7Z4T6

UniProtKB/Swiss-Prot

P08651

Conserved Domains (3) summary

pfam00859
Location:217 – 508
Blast Score: 775

CTF_NFI; CTF/NF-I family transcription modulation region

pfam10524
Location:4 – 47
Blast Score: 225

NfI_DNAbd_pre-N; Nuclear factor I protein pre-N-terminus

cl00055
Location:68 – 169
Blast Score: 191

MH1; N-terminal Mad Homology 1 (MH1) domain
NM_001245004.1 → NP_001231933.1 nuclear factor 1 C-type isoform 3

Status: REVIEWED

Description

Transcript Variant: This variant (3) lacks an exon in the 3' coding region compared to variant 1. This results in a frame-shift, and a shorter isoform (3) with a distinct C-terminus compared to isoform 1.

Source sequence(s)

AC005551, AK289885, BC012120, BF763988, BU727954, CB053896, CK431049

UniProtKB/Swiss-Prot

P08651

Conserved Domains (3) summary

pfam00859
Location:217 – 418
Blast Score: 608

CTF_NFI; CTF/NF-I family transcription modulation region

pfam10524
Location:4 – 47
Blast Score: 224

NfI_DNAbd_pre-N; Nuclear factor I protein pre-N-terminus

cl00055
Location:68 – 169
Blast Score: 188

MH1; N-terminal Mad Homology 1 (MH1) domain
NM_001245005.1 → NP_001231934.1 nuclear factor 1 C-type isoform 4

Status: REVIEWED

Description

Transcript Variant: This variant (4) contains an alternate 5' terminal exon, and lacks an exon in the 3' coding region (causing a frame-shift) compared to variant 1. This results in translation initiation from an alternate start codon, and a shorter isoform (4) with distinct N- and C- termini compared to isoform 1.

Source sequence(s)

AC005551, AK289885, BP279194, BU727954, CB053896, CK431049

UniProtKB/Swiss-Prot

P08651

Conserved Domains (3) summary

pfam00859
Location:208 – 409
Blast Score: 611

CTF_NFI; CTF/NF-I family transcription modulation region

pfam10524
Location:2 – 38
Blast Score: 203

NfI_DNAbd_pre-N; Nuclear factor I protein pre-N-terminus

cl00055
Location:59 – 160
Blast Score: 189

MH1; N-terminal Mad Homology 1 (MH1) domain
NM_005597.3 → NP_005588.2 nuclear factor 1 C-type isoform 5

Status: REVIEWED

Description

Transcript Variant: This variant (5) lacks two consecutive in-frame coding exons compared to variant 1. This results in a shorter isoform (5) missing an internal protein segment compared to isoform 1.

Source sequence(s)

AC005551, AK289885, BC012120, BF763988, BU727954, CB053896, CK431049

Consensus CDS

CCDS12107.1

UniProtKB/Swiss-Prot

P08651

Related

ENSP00000342194, ENST00000341919

Conserved Domains (3) summary

pfam00859
Location:217 – 423
Blast Score: 621

CTF_NFI; CTF/NF-I family transcription modulation region

pfam10524
Location:4 – 47
Blast Score: 223

NfI_DNAbd_pre-N; Nuclear factor I protein pre-N-terminus

cl00055
Location:68 – 169
Blast Score: 188

MH1; N-terminal Mad Homology 1 (MH1) domain
NM_205843.2 → NP_995315.1 nuclear factor 1 C-type isoform 2

Status: REVIEWED

Description

Transcript Variant: This variant (2) contains an alternate 5' terminal exon compared to variant 1. This results in translation initiation from an alternate start codon, and a shorter isoform (2) with a distinct N-terminus compared to isoform 1.

Source sequence(s)

AC005551, AK289885, BP279194, BU727954, CB053896, CK431049

Consensus CDS

CCDS45914.1

UniProtKB/Swiss-Prot

P08651

Related

ENSP00000396843, ENST00000443272

Conserved Domains (3) summary

pfam00859
Location:208 – 499
Blast Score: 779

CTF_NFI; CTF/NF-I family transcription modulation region

pfam10524
Location:2 – 38
Blast Score: 205

NfI_DNAbd_pre-N; Nuclear factor I protein pre-N-terminus

cl00055
Location:59 – 160
Blast Score: 191

MH1; N-terminal Mad Homology 1 (MH1) domain

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000019.9 Reference GRCh37.p5 Primary Assembly

Range

3359616..3463603

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000151.1 Alternate HuRef

Range

3125655..3229498

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Help top of page

Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AC005514.1	AAC32593.1
		AAC32594.1
genomic	AC005551.1	AAC33190.1
		AAC33191.1
		AAC33192.1
genomic	AC005778.1	AAC62842.1
genomic	AC007792.1 (36091..37654)	None
genomic	AC093077.2 (1136..4284)	None
genomic	AC104104.3 (1146..11173)	None
genomic	CH471139.2	EAW69323.1
		EAW69324.1
		EAW69325.1
		EAW69326.1
mRNA	AK289885.1	BAF82574.1
mRNA	AK297825.1	BAH12672.1
mRNA	AK297867.1	BAH12681.1
mRNA	AK304816.1	BAH14259.1
mRNA	AL042511.1	None
mRNA	BC012120.1	AAH12120.1
mRNA	BE887429.1	None
mRNA	BF763988.1	None
mRNA	BI253961.1	None
mRNA	BP279194.1	None
mRNA	BQ894051.1	None
mRNA	BU727954.1	None
mRNA	CB053896.1	None
mRNA	CK431049.1	None
mRNA	CR536507.1	CAG38745.1
mRNA	X12492.1	CAA31012.1
mRNA	X92857.1	CAA63440.1
other-genetic	DQ893655.2	ABM84581.1
other-genetic	DQ895795.2	ABM86721.1