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    NASP nuclear autoantigenic sperm protein (histone-binding) [ Homo sapiens ]

    Gene ID: 4678, updated on 20-May-2012
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    Summary

    Official Symbol
    NASPprovided by HGNC
    Official Full Name
    nuclear autoantigenic sperm protein (histone-binding)provided by HGNC
    Primary source
    HGNC:7644
    Locus tag
    RP4-697E16.3
    See related
    Ensembl:ENSG00000132780; HPRD:04423; MIM:603185; Vega:OTTHUMG00000007826
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    FLB7527; PRO1999; MGC2297; FLJ31599; FLJ35510; MGC19722; MGC20372; DKFZp547F162
    Summary
    This gene encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa. [provided by RefSeq, Jul 2008]
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    Genomic context

    Location :
    1p34.1
    Sequence :
    Chromosome: 1; NC_000001.10 (46049660..46084578)
    See NASP in Epigenomics, MapViewer

    Chromosome 1 - NC_000001.10Genomic Context describing neighboring genes Neighboring gene high mobility group box 1 pseudogene 48 Neighboring gene aldo-keto reductase family 1, member A1 (aldehyde reductase) Neighboring gene coiled-coil domain containing 17 Neighboring gene GC-rich promoter binding protein 1-like 1 Neighboring gene ribosomal protein S15a pseudogene 10

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    Genomic regions, transcripts, and products

    Go to nucleotideGraphics FASTA GenBank

    Go to reference sequence details
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    Bibliography

    Related articles in PubMed

    1. A specific function for the histone chaperone NASP to fine-tune a reservoir of soluble H3-H4 in the histone supply chain. Cook AJ, et al. Mol Cell, 2011 Dec 23. PMID 22195965.
    2. MicroRNA-29a inhibited epididymal epithelial cell proliferation by targeting nuclear autoantigenic sperm protein (NASP). Ma W, et al. J Biol Chem, 2012 Mar 23. PMID 22194605.
    3. Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels. Lee KA, et al. J Biol Chem, 2011 Dec 2. PMID 21987572.
    4. The human histone chaperone sNASP interacts with linker and core histones through distinct mechanisms. Wang H, et al. Nucleic Acids Res, 2012 Jan. PMID 21965532.
    5. Systematic and quantitative assessment of the ubiquitin-modified proteome. Kim W, et al. Mol Cell, 2011 Oct 21. PMID 21906983.

    See all (47) citations in PubMed

    See citations in PubMed for homologs of this gene provided by HomoloGene

    GeneRIFs: Gene References Into Functions What's a GeneRIF?

    1. an increase of miR-29a, and hence decrease of Nasp, may contribute to inhibit cell proliferation during postnatal organ development.
      Title: MicroRNA-29a inhibited epididymal epithelial cell proliferation by targeting nuclear autoantigenic sperm protein (NASP).
    2. sNASP interacts with linker and core histones through distinct structural domains.
      Title: The human histone chaperone sNASP interacts with linker and core histones through distinct mechanisms.
    3. The insights into NASP function and the existence of a tunable reservoir in mammalian cells demonstrate that contingency is integrated into the histone supply chain to respond to unexpected changes in demand.
      Title: A specific function for the histone chaperone NASP to fine-tune a reservoir of soluble H3-H4 in the histone supply chain.
    4. tNASP is critical for the survival of prostate cancer cells; targeting tNASP expression can lead to a new approach for prostate cancer treatment.
      Title: Depletion of the histone chaperone tNASP inhibits proliferation and induces apoptosis in prostate cancer PC-3 cells.
    5. NASP and RCAS1 proteins were more frequently expressed in ovarian cancer tissues than with normal ovarian tissue and serous cystadenomas and MRE11 was less frequently expressed
      Title: Analysis of the expression of human tumor antigens in ovarian cancer tissues.
    6. A deletion analysis of sNASP revealed that the central region, amino acid residues 26-325, of sNASP is responsible for nucleosome assembly in vitro.
      Title: Nucleosome formation activity of human somatic nuclear autoantigenic sperm protein (sNASP).
    7. NASP belongs to a network of genes and gene functions that are critical for cell survival
      Title: Analysis of gene expression profiles in HeLa cells in response to overexpression or siRNA-mediated depletion of NASP.
    8. NASP forms distinct, high specificity complexes with histones H3 and H4.
      Title: Expanded binding specificity of the human histone chaperone NASP.
    9. NASP and the linker histones are key players in the assembly of chromatin after DNA replication
      Title: Nuclear autoantigenic sperm protein (NASP), a linker histone chaperone that is required for cell proliferation.
    Submit: New GeneRIF Correction
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    Phenotypes

    Review eQTL and phenotype association data in this region using PheGenI

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    Interactions

    Products Interactant Other Gene Complex Source Pubs Description
    P49321 Q02539 HIST1H1A    HPRD  PubMed  
    P49321 P16403 HIST1H1C    HPRD  PubMed  
    P49321 Histone 1 H3g HIST1H3G    HPRD  PubMed  
    P49321 Histone 1 H3h HIST1H3H    HPRD  PubMed  
    P49321 Q71DI3 HIST2H3C    HPRD  PubMed  
    P49321 Q9Y383 LUC7L2    HPRD  PubMed  
    P49321 Q8IYT8 ULK2    HPRD  PubMed  
    BioGRID:110759 BioGRID:106823 AIRE    BioGRID  PubMed Affinity Capture-MS; Affinity Capture-Western 
    NP_054753.1 ASF1A    BIND  PubMed Asf1a interacts with NASP. This interaction was modeled on a demonstrated interaction between Asf1a from an unspecified species and human NASP. 
    BioGRID:110759 BioGRID:109272 H3F3A    BioGRID  PubMed Affinity Capture-MS; Affinity Capture-Western 
    BioGRID:110759 BioGRID:115330 HDAC6    BioGRID  PubMed Two-hybrid 
    BioGRID:110759 BioGRID:113946 HIST1H3A    BioGRID  PubMed Affinity Capture-MS; Reconstituted Complex 
    BioGRID:110759 BioGRID:113949 HIST1H3E    BioGRID  PubMed Affinity Capture-MS; Affinity Capture-Western 
    BioGRID:110759 BioGRID:113951 HIST1H3G    BioGRID  PubMed Two-hybrid 
    BioGRID:110759 BioGRID:113953 HIST1H3H    BioGRID  PubMed Two-hybrid 
    BioGRID:110759 BioGRID:113955 HIST1H4A    BioGRID  PubMed Reconstituted Complex 
    BioGRID:110759 BioGRID:126025 HIST2H3C    BioGRID  PubMed Two-hybrid 
    BioGRID:110759 BioGRID:119646 LUC7L2    BioGRID  PubMed Two-hybrid 
    BioGRID:110759 BioGRID:204968 Mapk13    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:110759 BioGRID:111719 PTK2    BioGRID  PubMed Two-hybrid 
    BioGRID:110759 BioGRID:112497 SUMO2    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:110759 BioGRID:113041 TRAF6    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:110759 BioGRID:113164 UBC    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:110759 BioGRID:115058 ULK2    BioGRID  PubMed Two-hybrid 
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    General gene information

    Markers

    Genotypes

    Homology

    • Homologs of the NASP gene: The NASP gene is conserved in chimpanzee, , dog, cow, mouse, rat, chicken, zebrafish, C.elegans, K.lactis, S.pombe, , and N.crassa.
    • Map Viewer (Mouse, Rat)

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    Hsp90 protein binding ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    histone binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    DNA replication ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    blastocyst development ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    cell cycle ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    cell proliferation ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    histone exchange ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    protein transport IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    nucleus ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
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    General protein information

    Preferred Names
    nuclear autoantigenic sperm protein
    Names
    nuclear autoantigenic sperm protein
    histone H1-binding protein
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    NCBI Reference Sequences (RefSeq)

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    Genomic

    1. NG_029224.1 RefSeqGene

      Range
      5001..39919
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001195193.1 → NP_001182122.1  nuclear autoantigenic sperm protein isoform 4

      Status: REVIEWED

      Description
      Transcript Variant: This variant (4) lacks two alternate in-frame exons in the coding region, compared to variant 2. This results in a shorter protein (isoform 4), compared to isoform 2.
      Source sequence(s)
      AK291637, AK298893, BC010105, CK300084, CX781565, DC361634
      Consensus CDS
      CCDS55597.1
      UniProtKB/TrEMBL
      B4DQP3
      UniProtKB/TrEMBL
      F5H3J2
      UniProtKB/Swiss-Prot
      P49321
      Related
      ENSP00000438871, ENST00000537798
      Conserved Domains (3) summary
      cd00189
      Location:456 – 552
      Blast Score: 104
      TPR; Tetratricopeptide repeat domain; typically contains 34 amino acids [WLF]-X(2)-[LIM]-[GAS]-X(2)-[YLF]-X(8)-[ASE]-X(3)-[FYL]-X(2)-[ASL]-X(4)-[PKE] is the consensus sequence; found in a variety of organisms including bacteria, cyanobacteria, yeast, fungi, ...
      COG5271
      Location:63 – 260
      Blast Score: 89
      MDN1; AAA ATPase containing von Willebrand factor type A (vWA) domain [General function prediction only]
      pfam13424
      Location:475 – 552
      Blast Score: 118
      TPR_12; Tetratricopeptide repeat

    2. NM_002482.3 → NP_002473.2  nuclear autoantigenic sperm protein isoform 2

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2).
      Source sequence(s)
      AK291637, BC010105, CK300084, CX781565, DC361634
      Consensus CDS
      CCDS524.1
      UniProtKB/Swiss-Prot
      P49321
      Related
      ENSP00000255120, OTTHUMP00000009513, ENST00000350030, OTTHUMT00000021533
      Conserved Domains (4) summary
      COG5271
      Location:127 – 324
      Blast Score: 91
      MDN1; AAA ATPase containing von Willebrand factor type A (vWA) domain [General function prediction only]
      pfam10516
      Location:542 – 579
      Blast Score: 131
      SHNi-TPR; SHNi-TPR
      pfam13424
      Location:539 – 616
      Blast Score: 116
      TPR_12; Tetratricopeptide repeat
      cl02429
      Location:584 – 616
      Blast Score: 84
      TPR; Tetratricopeptide repeat domain; typically contains 34 amino acids [WLF]-X(2)-[LIM]-[GAS]-X(2)-[YLF]-X(8)-[ASE]-X(3)-[FYL]-X(2)-[ASL]-X(4)-[PKE] is the consensus sequence; found in a variety of organisms including bacteria, cyanobacteria, yeast, fungi, ...

    3. NM_152298.3 → NP_689511.2  nuclear autoantigenic sperm protein isoform 3

      Status: REVIEWED

      Description
      Transcript Variant: This variant (3) lacks an alternate in-frame exon in the coding region, compared to variant 2. This results in a shorter protein (isoform 3), compared to isoform 2.
      Source sequence(s)
      AK291637, BC053608, CK300084, CX781565, DC361634
      Consensus CDS
      CCDS525.1
      UniProtKB/Swiss-Prot
      P49321
      UniProtKB/TrEMBL
      Q5T626
      Related
      ENSP00000255121, OTTHUMP00000009509, ENST00000351223, OTTHUMT00000021526
      Conserved Domains (3) summary
      pfam10516
      Location:203 – 240
      Blast Score: 133
      SHNi-TPR; SHNi-TPR
      pfam13424
      Location:200 – 277
      Blast Score: 107
      TPR_12; Tetratricopeptide repeat
      cl02429
      Location:245 – 277
      Blast Score: 79
      TPR; Tetratricopeptide repeat domain; typically contains 34 amino acids [WLF]-X(2)-[LIM]-[GAS]-X(2)-[YLF]-X(8)-[ASE]-X(3)-[FYL]-X(2)-[ASL]-X(4)-[PKE] is the consensus sequence; found in a variety of organisms including bacteria, cyanobacteria, yeast, fungi, ...

    RefSeqs of Annotated Genomes: Build 37.3

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh37.p5 Primary Assembly

    Genomic

    1. NC_000001.10 Reference GRCh37.p5 Primary Assembly

      Range
      46049660..46084578
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate HuRef

    Genomic

    1. AC_000133.1 Alternate HuRef

      Range
      44161002..44195831
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Suppressed Reference Sequence(s)

    The following Reference Sequences have been suppressed. Explain

    1. NM_172164.1: Suppressed sequence

      Description
      NM_172164.1: This RefSeq was permanently suppressed because it is a nonsense-mediated mRNA decay (NMD) candidate.
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    Related Sequences

    Nucleotide Protein
    Heading Accession and Version
    genomic AL355480.22 CAI22460.1
      CAI22461.1
      CAI22462.1
      CAI22463.1
      CAI22464.1
      CAI22465.1
    genomic CH471059.2 EAX06964.1
      EAX06965.1
      EAX06966.1
      EAX06967.1
      EAX06968.1
      EAX06969.1
    mRNA AF035191.1 AAD02006.1
    mRNA AK056161.1 None
    mRNA AK092829.1 None
    mRNA AK222778.1 BAD96498.1
    mRNA AK222816.1 BAD96536.1
    mRNA AK291637.1 BAF84326.1
    mRNA AK298893.1 BAG61005.1
    mRNA AK299584.1 BAG61519.1
    mRNA AK308001.1 None
    mRNA AL512718.1 None
    mRNA AL571577.2 None
    mRNA BC003113.1 AAH03113.1
    mRNA BC009933.2 AAH09933.1
    mRNA BC010105.2 AAH10105.1
    mRNA BC011580.1 AAH11580.1
    mRNA BC053608.1 AAH53608.1
    mRNA BT006757.1 AAP35403.1
    mRNA CK300084.1 None
    mRNA CX781565.1 None
    mRNA DC361634.1 None
    mRNA M97856.1 AAA36027.1
    other-genetic DQ893275.2 ABM84201.1
    other-genetic DQ896606.2 ABM87605.1
    Protein Accession Links
    GenPept Link UniProtKB Link
    O95138 GenPept UniProtKB/TrEMBL:O95138
    P49321.2 GenPept UniProtKB/Swiss-Prot:P49321
    Q53H03 GenPept UniProtKB/TrEMBL:Q53H03
    Q5T623 GenPept UniProtKB/TrEMBL:Q5T623
    Q5T624 GenPept UniProtKB/TrEMBL:Q5T624
    Q5T626 GenPept UniProtKB/TrEMBL:Q5T626
    Q5T627 GenPept UniProtKB/TrEMBL:Q5T627
    Q6PJY7 GenPept UniProtKB/TrEMBL:Q6PJY7
    Q9P1N1 GenPept UniProtKB/TrEMBL:Q9P1N1
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    Additional Links

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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