ASNS asparagine synthetase (glutamine-hydrolyzing) [Homo sapiens] - Gene

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Summary

Official Symbol: ASNSprovided by HGNC
Official Full Name: asparagine synthetase (glutamine-hydrolyzing)provided by HGNC
Primary source: HGNC:753
See related: Ensembl:ENSG00000070669; HPRD:00153; MIM:108370; Vega:OTTHUMG00000022892
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: TS11
Summary: The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

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Genomic context

Location :: 7q21.3

Sequence :: Chromosome: 7; NC_000007.13 (97481429..97501854, complement)

See ASNS in Epigenomics, MapViewer

Chromosome 7 - NC_000007.13 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

AsnS mRNA expression level in tumor cells reflects the sensitivity of cells to L-asparaginase.
Title: [Relationship between asparagine synthetase expression level and cell sensitivity to L-asparaginase in human leukemic cell lines].
High expression of asparagine synthetase is associated with a poor outcome in ALL children.
Title: [Study of the correlation between the expression level of asparagine synthetase and the outcome of children with acute lymphocytic leukemia].
Declined asparagine synthetase mRNA expression and enhanced sensitivity to asparaginase in HL-60 cells committed to monocytic differentiation.
Title: Declined asparagine synthetase mRNA expression and enhanced sensitivity to asparaginase in HL-60 cells committed to monocytic differentiation.
DNA polymorphism of a tandem repeated sequence is associated with the asparagine synthetase gene in acute lymphoblastic leukemia
Title: Functional analysis of a novel DNA polymorphism of a tandem repeated sequence in the asparagine synthetase gene in acute lymphoblastic leukemia cells.
CHOP is a member of the transcription factor network that controls the stress-induced regulation of specific C/EBP-ATF4-containing genes, such as ASNS
Title: C/EBP homology protein (CHOP) interacts with activating transcription factor 4 (ATF4) and negatively regulates the stress-dependent induction of the asparagine synthetase gene.
ASNS (asparagine synthetase) transcription activity was up-regulated in HepG2 cells treated with the UPR activators thapsigargin and tunicamycin
Title: Transcriptional induction of the human asparagine synthetase gene during the unfolded protein response does not require the ATF6 and IRE1/XBP1 arms of the pathway.
These findings provide rationale for evaluation of ASNS protein expression as a predictive biomarker of clinical L-ASP activity in ovarian cancer.
Title: Asparagine synthetase is a predictive biomarker of L-asparaginase activity in ovarian cancer cell lines.
ASNS protein expression predicts L-asparaginase chemotherapeutic activity in a large, diverse set of ovarian cancer cell lines.
Title: Asparagine synthetase is a predictive biomarker of L-asparaginase activity in ovarian cancer cell lines.
ASNS RNAi potentiates L-asparaginase chemotherapeutic activity in ovarian cancer cell lines from the NCI-60, and ASNS mRNA expression predicts L-asparaginase activity in the ovarian subpanel of the NCI-60.
Title: Asparagine synthetase as a causal, predictive biomarker for L-asparaginase activity in ovarian cancer cells.
coorelation between methylation of ASNS and acute lymphoblastic leukemia
Title: Methylation analysis of asparagine synthetase gene in acute lymphoblastic leukemia cells.

Submit: New GeneRIF Correction See all (18)

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Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

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Products	Interactant	Other Gene	Source	Pubs	Description
P08243	Q9NRL3	STRN4	HPRD	PubMed
BioGRID:106932	BioGRID:121948	C12orf44	BioGRID	PubMed	Affinity Capture-MS
BioGRID:106932	BioGRID:122485	DDA1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:106932	BioGRID:119531	PRKAG2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:106932	BioGRID:118941	STRN4	BioGRID	PubMed	Two-hybrid
BioGRID:106932	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS

Function	Evidence Code	Pubs
ATP binding	IEA Inferred from Electronic Annotation more info
asparagine synthase (glutamine-hydrolyzing) activity	EXP Inferred from Experiment more info
asparagine synthase (glutamine-hydrolyzing) activity	IDA Inferred from Direct Assay more info	PubMed
cofactor binding	IEA Inferred from Electronic Annotation more info
ligase activity	IEA Inferred from Electronic Annotation more info
nucleotide binding	IEA Inferred from Electronic Annotation more info
protein homodimerization activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
activation of signaling protein activity involved in unfolded protein response	TAS Traceable Author Statement more info
asparagine biosynthetic process	IC Inferred by Curator more info	PubMed
asparagine biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
cellular amino acid biosynthetic process	TAS Traceable Author Statement more info
cellular nitrogen compound metabolic process	TAS Traceable Author Statement more info
cellular response to glucose starvation	IDA Inferred from Direct Assay more info	PubMed
cellular response to hormone stimulus	IEA Inferred from Electronic Annotation more info
endoplasmic reticulum unfolded protein response	TAS Traceable Author Statement more info
glutamine metabolic process	IEA Inferred from Electronic Annotation more info
liver development	IEA Inferred from Electronic Annotation more info
negative regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
positive regulation of mitotic cell cycle	IDA Inferred from Direct Assay more info	PubMed
response to amino acid stimulus	IEA Inferred from Electronic Annotation more info
response to follicle-stimulating hormone stimulus	IEA Inferred from Electronic Annotation more info
response to light stimulus	IEA Inferred from Electronic Annotation more info
response to mechanical stimulus	IEA Inferred from Electronic Annotation more info
response to methotrexate	IEA Inferred from Electronic Annotation more info
response to nutrient levels	IEA Inferred from Electronic Annotation more info
response to toxin	IEA Inferred from Electronic Annotation more info
small molecule metabolic process	TAS Traceable Author Statement more info

Component	Evidence Code	Pubs
cytosol	TAS Traceable Author Statement more info
soluble fraction	IDA Inferred from Direct Assay more info	PubMed

General protein information

Preferred Names: asparagine synthetase [glutamine-hydrolyzing]

Names: asparagine synthetase [glutamine-hydrolyzing]; TS11 cell cycle control protein; glutamine-dependent asparagine synthetase

NP_001171546.1

EC 6.3.5.4

NP_001171547.1

EC 6.3.5.4

NP_001171548.1

EC 6.3.5.4

NP_001664.3

EC 6.3.5.4

NP_597680.2

EC 6.3.5.4

NP_899199.2

EC 6.3.5.4

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NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001178075.1 → NP_001171546.1 asparagine synthetase [glutamine-hydrolyzing] isoform b

Status: REVIEWED

Description

Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (b) is shorter than isoform a.

Source sequence(s)

AA948141, AK302189, BC014621, DC297828

Consensus CDS

CCDS55132.1

UniProtKB/TrEMBL

B4DXZ1

UniProtKB/TrEMBL

E9PCI3

UniProtKB/Swiss-Prot

P08243

Related

ENSP00000406994, OTTHUMP00000204939, ENST00000444334, OTTHUMT00000333643

Conserved Domains (3) summary

cd01991
Location:215 – 452
Blast Score: 359

Asn_Synthase_B_C; The C-terminal domain of Asparagine Synthase B. This domain is always found associated n-terminal amidotransferase domain. Family members that contain this domain catalyse the conversion of aspartate to asparagine. Asparagine synthetase B catalyzes the ...

cd00712
Location:1 – 170
Blast Score: 411

AsnB; Glutamine amidotransferases class-II (GATase) asparagine synthase_B type. Asparagine synthetase B catalyses the ATP-dependent conversion of aspartate to asparagine. This enzyme is a homodimer, with each monomer composed of a glutaminase domain and a ...

PRK09431
Location:2 – 534
Blast Score: 1264

asnB; asparagine synthetase B; Provisional
NM_001178076.1 → NP_001171547.1 asparagine synthetase [glutamine-hydrolyzing] isoform c

Status: REVIEWED

Description

Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (c) is shorter than isoform a. Variants 5 and 6 encode the same isoform (c).

Source sequence(s)

AA948141, BC014621, DA160359, DC297828

Consensus CDS

CCDS55131.1

UniProtKB/TrEMBL

E9PCX6

UniProtKB/Swiss-Prot

P08243

Related

ENSP00000408472, OTTHUMP00000204940, ENST00000455086, OTTHUMT00000333644

Conserved Domains (2) summary

cd01991
Location:153 – 390
Blast Score: 355

Asn_Synthase_B_C; The C-terminal domain of Asparagine Synthase B. This domain is always found associated n-terminal amidotransferase domain. Family members that contain this domain catalyse the conversion of aspartate to asparagine. Asparagine synthetase B catalyzes the ...

cl00319
Location:8 – 108
Blast Score: 235

Gn_AT_II; Glutamine amidotransferases class-II (GATase). The glutaminase domain catalyzes an amide nitrogen transfer from glutamine to the appropriate substrate. In this process, glutamine is hydrolyzed to glutamic acid and ammonia. This domain is related to ...
NM_001178077.1 → NP_001171548.1 asparagine synthetase [glutamine-hydrolyzing] isoform c

Status: REVIEWED

Description

Transcript Variant: This variant (6) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (c) is shorter than isoform a. Variants 5 and 6 encode the same isoform (c).

Source sequence(s)

AA948141, BC014621, DB483248

Consensus CDS

CCDS55131.1

UniProtKB/TrEMBL

E9PCX6

UniProtKB/Swiss-Prot

P08243

Related

ENSP00000414379, OTTHUMP00000204942, ENST00000437628, OTTHUMT00000333646

Conserved Domains (2) summary

cd01991
Location:153 – 390
Blast Score: 355

Asn_Synthase_B_C; The C-terminal domain of Asparagine Synthase B. This domain is always found associated n-terminal amidotransferase domain. Family members that contain this domain catalyse the conversion of aspartate to asparagine. Asparagine synthetase B catalyzes the ...

cl00319
Location:8 – 108
Blast Score: 235

Gn_AT_II; Glutamine amidotransferases class-II (GATase). The glutaminase domain catalyzes an amide nitrogen transfer from glutamine to the appropriate substrate. In this process, glutamine is hydrolyzed to glutamic acid and ammonia. This domain is related to ...
NM_001673.4 → NP_001664.3 asparagine synthetase [glutamine-hydrolyzing] isoform a

Status: REVIEWED

Description

Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same isoform (a).

Source sequence(s)

AA948141, BC014621, DC297828

Consensus CDS

CCDS5652.1

UniProtKB/Swiss-Prot

P08243

Related

ENSP00000377845, OTTHUMP00000024510, ENST00000394308, OTTHUMT00000059213

Conserved Domains (3) summary

cd01991
Location:236 – 473
Blast Score: 360

Asn_Synthase_B_C; The C-terminal domain of Asparagine Synthase B. This domain is always found associated n-terminal amidotransferase domain. Family members that contain this domain catalyse the conversion of aspartate to asparagine. Asparagine synthetase B catalyzes the ...

cd00712
Location:2 – 191
Blast Score: 439

AsnB; Glutamine amidotransferases class-II (GATase) asparagine synthase_B type. Asparagine synthetase B catalyses the ATP-dependent conversion of aspartate to asparagine. This enzyme is a homodimer, with each monomer composed of a glutaminase domain and a ...

PRK09431
Location:1 – 555
Blast Score: 1287

asnB; asparagine synthetase B; Provisional
NM_133436.3 → NP_597680.2 asparagine synthetase [glutamine-hydrolyzing] isoform a

Status: REVIEWED

Description

Transcript Variant: This variant (1) is the longest variant. Variants 1, 2 and 3 encode the same isoform (a).

Source sequence(s)

AA948141, BC014621, DA961373

Consensus CDS

CCDS5652.1

UniProtKB/Swiss-Prot

P08243

Related

ENSP00000377846, OTTHUMP00000204941, ENST00000394309, OTTHUMT00000333645

Conserved Domains (3) summary

cd01991
Location:236 – 473
Blast Score: 360

Asn_Synthase_B_C; The C-terminal domain of Asparagine Synthase B. This domain is always found associated n-terminal amidotransferase domain. Family members that contain this domain catalyse the conversion of aspartate to asparagine. Asparagine synthetase B catalyzes the ...

cd00712
Location:2 – 191
Blast Score: 439

AsnB; Glutamine amidotransferases class-II (GATase) asparagine synthase_B type. Asparagine synthetase B catalyses the ATP-dependent conversion of aspartate to asparagine. This enzyme is a homodimer, with each monomer composed of a glutaminase domain and a ...

PRK09431
Location:1 – 555
Blast Score: 1287

asnB; asparagine synthetase B; Provisional
NM_183356.3 → NP_899199.2 asparagine synthetase [glutamine-hydrolyzing] isoform a

Status: REVIEWED

Description

Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same isoform (a).

Source sequence(s)

AA948141, BC014621, DA160359, DC297828

Consensus CDS

CCDS5652.1

UniProtKB/Swiss-Prot

P08243

Related

ENSP00000175506, ENST00000175506

Conserved Domains (3) summary

cd01991
Location:236 – 473
Blast Score: 360

Asn_Synthase_B_C; The C-terminal domain of Asparagine Synthase B. This domain is always found associated n-terminal amidotransferase domain. Family members that contain this domain catalyse the conversion of aspartate to asparagine. Asparagine synthetase B catalyzes the ...

cd00712
Location:2 – 191
Blast Score: 439

AsnB; Glutamine amidotransferases class-II (GATase) asparagine synthase_B type. Asparagine synthetase B catalyses the ATP-dependent conversion of aspartate to asparagine. This enzyme is a homodimer, with each monomer composed of a glutaminase domain and a ...

PRK09431
Location:1 – 555
Blast Score: 1287

asnB; asparagine synthetase B; Provisional

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000007.13 Reference GRCh37.p5 Primary Assembly

Range

97481429..97501854, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000139.1 Alternate HuRef

Range

92082173..92102489, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate CRA_TCAGchr7v2

Genomic

AC_000068.1 Alternate CRA_TCAGchr7v2

Range

96803932..96824039, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

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Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AC005326.2 (78683..99039)	None
genomic	AC079781.7	AAQ96856.1
genomic	CH236949.1	EAL24115.1
genomic	CH471091.2	EAW76723.1
		EAW76730.1
		EAW76731.1
		EAW76732.1
		EAW76733.1
genomic	L35946.1	AAA52756.1
genomic	M27054.1	AAA63266.1
mRNA	AA948141.1	None
mRNA	AK000379.1	None
mRNA	AK302189.1	BAG63553.1
mRNA	AK302242.1	BAG63594.1
mRNA	AK316224.1	BAH14595.1
mRNA	BC008723.2	AAH08723.1
mRNA	BC014621.2	AAH14621.1
mRNA	BG718826.1	None
mRNA	BT007113.1	AAP35777.1
mRNA	BX104871.1	None
mRNA	DA160359.1	None
mRNA	DA661624.1	None
mRNA	DA961373.1	None
mRNA	DB483248.1	None
mRNA	DC297828.1	None
mRNA	M15798.1	AAA36781.1
mRNA	M27396.1	AAA51789.1
other-genetic	DQ893018.2	ABM83944.1
other-genetic	DQ896263.2	ABM87262.1