ASNA1 arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) [Homo sapiens] - Gene

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Summary

Official Symbol: ASNA1provided by HGNC
Official Full Name: arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)provided by HGNC
Primary source: HGNC:752
See related: Ensembl:ENSG00000198356; HPRD:03555; MIM:601913
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: GET3; ARSA1; TRC40; ARSA-I; ASNA-I; hASNA-I; MGC3821
Summary: This gene represents the human homolog of the bacterial arsA gene, encoding the arsenite-stimulated ATPase component of the arsenite transporter responsible for resistance to arsenicals. This protein is also a central component of a transmembrane domain (TMD) recognition complex (TRC) that is involved in the post-translational delivery of tail-anchored (TA) proteins from the cytosol to the endoplasmic reticulum (ER). It recognizes and selectively binds the TMD of TA proteins in the cytosol, and delivers them to the ER for insertion. [provided by RefSeq, Oct 2011]

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Genomic context

Location :: 19q13.3

Sequence :: Chromosome: 19; NC_000019.9 (12848306..12859137)

See ASNA1 in Epigenomics, MapViewer

Chromosome 19 - NC_000019.9 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Post-translational membrane insertion of tail-anchored transmembrane EF-hand Ca2+ sensor calneurons requires the TRC40/Asna1 protein chaperone.
Title: Post-translational membrane insertion of tail-anchored transmembrane EF-hand Ca2+ sensor calneurons requires the TRC40/Asna1 protein chaperone.
The coiled-coil domain of WRB is the binding site for TRC40/Asna1.
Title: WRB is the receptor for TRC40/Asna1-mediated insertion of tail-anchored proteins into the ER membrane.
Asna1 is required for efficient histocompatibility (HLA) class I downregulation mediated by viral protein BNLF2a.
Title: EBV protein BNLF2a exploits host tail-anchored protein integration machinery to inhibit TAP.
Asna1 can mediate membrane insertion of RAMP4 and Sec61beta without the participation of other cytosolic proteins by a mechanism that depends on the presence of ATP or ADP and a protease-sensitive receptor in the endoplasmic reticulum membrane.
Title: Asna1/TRC40-mediated membrane insertion of tail-anchored proteins.
ASNA1 is a target to overcome platinum resistance in ovarian cancer
Title: Increased sensitivity to platinating agents and arsenite in human ovarian cancer by downregulation of ASNA1.
the hydrophobicity of the TA region dictates whether a precursor is delivered to the ER via the Hsp40/Hsc70 or Asna-1/TRC40-dependent route.
Title: A precursor-specific role for Hsp40/Hsc70 during tail-anchored protein integration at the endoplasmic reticulum.
Human ASNA1 is highly expressed in pancreatic beta cells, but not in other pancreatic endocrine cell types, and regulates insulin secretion in cultured cells.
Title: ASNA-1 positively regulates insulin secretion in C. elegans and mammalian cells.
TRC40/Asna-1 interacts posttranslationally with tail-anchored proteins in a transmembrane domain-dependent manner for delivery to a proteinaceous receptor at the endoplasmic reticulum membrane.
Title: Identification of a targeting factor for posttranslational membrane protein insertion into the ER.

Submit: New GeneRIF Correction

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Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

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Products	Interactant	Other Gene	Source	Pubs	Description
O43681	O43681	ASNA1	HPRD	PubMed
BioGRID:106931	BioGRID:107227	CACNA1A	BioGRID	PubMed	Two-hybrid
BioGRID:106931	BioGRID:111223	PFDN1	BioGRID	PubMed	Two-hybrid
BioGRID:106931	BioGRID:116152	SEC61B	BioGRID	PubMed	Affinity Capture-MS
BioGRID:106931	BioGRID:118079	SERP1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:106931	BioGRID:112938	TK1	BioGRID	PubMed	Two-hybrid
BioGRID:106931	BioGRID:113127	TTR	BioGRID	PubMed	Two-hybrid
BioGRID:106931	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS
BioGRID:106931	BioGRID:113885	UBL4A	BioGRID	PubMed	Affinity Capture-MS

Function	Evidence Code	Pubs
ATP binding	IEA Inferred from Electronic Annotation more info
ATPase activity	IEA Inferred from Electronic Annotation more info
arsenite transmembrane transporter activity	TAS Traceable Author Statement more info	PubMed
hydrolase activity	IEA Inferred from Electronic Annotation more info
metal ion binding	IEA Inferred from Electronic Annotation more info
nucleotide binding	IEA Inferred from Electronic Annotation more info
transporter activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
arsenite transport	TAS Traceable Author Statement more info	PubMed
detoxification of arsenic-containing substance	IEA Inferred from Electronic Annotation more info
transport	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
cytoplasm	IEA Inferred from Electronic Annotation more info
endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
nucleolus	IEA Inferred from Electronic Annotation more info
nucleus	IEA Inferred from Electronic Annotation more info
soluble fraction	TAS Traceable Author Statement more info	PubMed

General protein information

Preferred Names: ATPase ASNA1

Names: ATPase ASNA1; arsenite-stimulated ATPase; arsenical pump-driving ATPase; golgi to ER traffic 3 homolog; transmembrane domain recognition complex, 40kDa; transmembrane domain recognition complex 40 kDa ATPase subunit

NP_004308.2

EC 3.6.3.16

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NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_004317.2 → NP_004308.2 ATPase ASNA1

Status: VALIDATED

Source sequence(s)

BC002651, BG426136

Consensus CDS

CCDS32920.1

UniProtKB/Swiss-Prot

O43681

Related

ENSP00000349887, ENST00000357332

Conserved Domains (2) summary

cd02035
Location:39 – 318
Blast Score: 506

ArsA; ArsA ATPase functionas as an efflux pump located on the inner membrane of the cell. This ATP-driven oxyanion pump catalyzes the extrusion of arsenite, antimonite and arsenate. Maintenance of a low intracellular concentration of oxyanion produces ...

pfam02374
Location:37 – 340
Blast Score: 1051

ArsA_ATPase; Anion-transporting ATPase

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000019.9 Reference GRCh37.p5 Primary Assembly

Range

12848306..12859137

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000151.1 Alternate HuRef

Range

12421428..12432073

Download

GenBank, FASTA, Sequence Viewer (Graphics)

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Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AC018761.6 (82209..93040)	None
genomic	AY304483.1	AAP45050.1
genomic	CH471106.1	EAW84303.1
mRNA	AF047469.1	AAC03551.1
mRNA	AK223363.1	BAD97083.1
mRNA	AK291855.1	BAF84544.1
mRNA	AK293588.1	BAG57056.1
mRNA	BC002651.2	AAH02651.1
mRNA	BG426136.1	None
mRNA	U60276.1	AAC50731.1
other-genetic	JF432148.1	ADZ15365.1