Miro Mitochondrial Rho [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Miroprovided by FlyBase
Official Full Name: Mitochondrial Rhoprovided by FlyBase
Primary source: FLYBASE:FBgn0039140
Locus tag: Dmel_CG5410
See related: AllianceGenome:FB:FBgn0039140
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: B682; CG5410; Dmel\CG5410; dmiro; dMiro; DMiro; DmMiro; miro; Q8IMX7-1; Q8IMX7-2
Summary: Enables GTP binding activity; calcium ion binding activity; and magnesium ion binding activity. Involved in axonal transport of mitochondrion; microtubule cytoskeleton organization; and synaptic vesicle transport. Predicted to be integral component of mitochondrial outer membrane. Is expressed in several structures, including adult head; extended germ band embryo; and germline cell. Used to study Parkinson's disease and neurodegenerative disease. Orthologous to human RHOT1 (ras homolog family member T1) and RHOT2 (ras homolog family member T2). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

Location:: 95D8-95D9; 3-82 cM

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (24034362..24038847, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (19860084..19864569, complement)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Our results highlight the importance of mitochondria-associated alpha-synuclein in human disease, and present Miro as a novel therapeutic target
Title: Alpha-synuclein delays mitophagy and targeting Miro rescues neuron loss in Parkinson's models.
A new mode of mitochondrial sorting in polarized cell growth, whereby bi-directional mitochondrial transport undertaken exclusively by dynein is regulated by Milton in the anterograde direction and by a Miro-dependent switch to the retrograde direction.
Title: A new mode of mitochondrial transport and polarized sorting regulated by Dynein, Milton and Miro.
we found that mitochondrial fission played a functional role in high-calcium induced necrosis, and a LOF vimar mutant rescued the mitochondrial fission defect and cell death.
Title: Vimar Is a Novel Regulator of Mitochondrial Fission through Miro.
Both conditions impaired NSC lineage progression. Ca(2) mito homeostasis is influenced by Polo-mediated phosphorylation of a conserved residue in Miro
Title: Polo Kinase Phosphorylates Miro to Control ER-Mitochondria Contact Sites and Mitochondrial Ca(2+) Homeostasis in Neural Stem Cell Development.
PINK1-mediated phosphorylation of Miro inhibits synaptic growth and protects dopaminergic neurons in Drosophila.
Title: PINK1-mediated phosphorylation of Miro inhibits synaptic growth and protects dopaminergic neurons in Drosophila.
The results of this study showed that dMiro's N-terminal GTPase domain is critically required for viability, mitochondrial size, and the distribution of mitochondria out of the neuronal soma.
Title: Miro's N-terminal GTPase domain is required for transport of mitochondria into axons and dendrites.
These results indicate structural mechanisms for calcium, nucleotide and phosphorylation-dependent regulation of mitochondrial function by Miro.
Title: Structural coupling of the EF hand and C-terminal GTPase domains in the mitochondrial protein Miro.
These newly identified functions of PINK1/Parkin and Miro in mitochondrial transport and mitophagy contribute to our understanding of the complex interplays in mitochondrial quality control that are critically involved in Parkinson's disease pathogenesis
Title: Parkinson's disease-associated kinase PINK1 regulates Miro protein level and axonal transport of mitochondria.
Promotes effective antero- and retrograde mitochondrial transport by extending the processivity of kinesin and dynein motors according to a mitochondrion's programmed direction of transport.
Title: Drosophila Miro is required for both anterograde and retrograde axonal mitochondrial transport.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables GTP binding	IBA Inferred from Biological aspect of Ancestor more info
enables GTP binding	IDA Inferred from Direct Assay more info	PubMed
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTPase activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activity	IEA Inferred from Electronic Annotation more info
enables GTPase activity	ISM Inferred from Sequence Model more info	PubMed
enables calcium ion binding	IDA Inferred from Direct Assay more info	PubMed
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables magnesium ion binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in axonal transport of mitochondrion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular homeostasis	ISS Inferred from Sequence or Structural Similarity more info
involved_in establishment of mitochondrion localization, microtubule-mediated	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microtubule cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrial outer membrane permeabilization	ISS Inferred from Sequence or Structural Similarity more info
involved_in mitochondrion localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitochondrion organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitochondrion organization	IEA Inferred from Electronic Annotation more info
involved_in mitochondrion transport along microtubule	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitochondrion transport along microtubule	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of mitochondrion organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in small GTPase-mediated signal transduction	IEA Inferred from Electronic Annotation more info
involved_in synaptic vesicle transport	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in mitochondrial outer membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrial outer membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrial outer membrane	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: mitochondrial Rho

Names: CG5410-PC; CG5410-PD; CG5410-PE; CG5410-PF; Miro-PC; Miro-PD; Miro-PE; Miro-PF; mitochondrial Rho-GTPase

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

24034362..24038847 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001275966.1 → NP_001262895.1 mitochondrial Rho, isoform F [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001262895.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4KGW0, I1WYI5

Conserved Domains (6) summary

smart00174
Location:14 → 195

RHO; Rho (Ras homology) subfamily of Ras-like small GTPases

cd01892
Location:444 → 620

Miro2; Mitochondrial Rho family 2 (Miro2), C-terminal

cd01893
Location:10 → 195

Miro1; Mitochondrial Rho family 1 (Miro1), N-terminal

pfam00071
Location:448 → 602

Ras; Ras family

pfam08355
Location:367 → 437

EF_assoc_1; EF hand associated

pfam08356
Location:247 → 328

EF_assoc_2; EF hand associated
NM_170111.3 → NP_732936.1 mitochondrial Rho, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_732936.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q8IMX6, Q8IMX7, Q95TT1

UniProtKB/TrEMBL

I1WYI5

Related

FBpp0083980

Conserved Domains (6) summary

smart00174
Location:14 → 195

RHO; Rho (Ras homology) subfamily of Ras-like small GTPases

cd01892
Location:444 → 615

Miro2; Mitochondrial Rho family 2 (Miro2), C-terminal

cd01893
Location:10 → 195

Miro1; Mitochondrial Rho family 1 (Miro1), N-terminal

pfam00071
Location:448 → 602

Ras; Ras family

pfam08355
Location:367 → 437

EF_assoc_1; EF hand associated

pfam08356
Location:247 → 328

EF_assoc_2; EF hand associated
NM_170114.3 → NP_732939.2 mitochondrial Rho, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_732939.2

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4J3Z9, I1WYI5

Conserved Domains (6) summary

smart00174
Location:14 → 195

RHO; Rho (Ras homology) subfamily of Ras-like small GTPases

cd01892
Location:444 → 620

Miro2; Mitochondrial Rho family 2 (Miro2), C-terminal

cd01893
Location:10 → 195

Miro1; Mitochondrial Rho family 1 (Miro1), N-terminal

pfam00071
Location:448 → 602

Ras; Ras family

pfam08355
Location:367 → 437

EF_assoc_1; EF hand associated

pfam08356
Location:247 → 328

EF_assoc_2; EF hand associated
NM_142948.4 → NP_651205.2 mitochondrial Rho, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_651205.2

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4KGW0, I1WYI5

Related

FBpp0083981

Conserved Domains (6) summary

smart00174
Location:14 → 195

RHO; Rho (Ras homology) subfamily of Ras-like small GTPases

cd01892
Location:444 → 620

Miro2; Mitochondrial Rho family 2 (Miro2), C-terminal

cd01893
Location:10 → 195

Miro1; Mitochondrial Rho family 1 (Miro1), N-terminal

pfam00071
Location:448 → 602

Ras; Ras family

pfam08355
Location:367 → 437

EF_assoc_1; EF hand associated

pfam08356
Location:247 → 328

EF_assoc_2; EF hand associated