Prat Phosphoribosylamidotransferase [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Pratprovided by FlyBase
Official Full Name: Phosphoribosylamidotransferaseprovided by FlyBase
Primary source: FLYBASE:FBgn0004901
Locus tag: Dmel_CG2867
See related: AllianceGenome:FB:FBgn0004901
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG2867; Dmel\CG2867; DmPrat
Summary: Enables amidophosphoribosyltransferase activity. Involved in several processes, including determination of adult lifespan; embryonic cleavage; and purine nucleotide biosynthetic process. Is expressed in several structures, including embryonic/larval lymph gland; extended germ band embryo; gonad; gut section; and presumptive embryonic/larval digestive system. Orthologous to human PPAT (phosphoribosyl pyrophosphate amidotransferase). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

Location:: 84E5-84E5; 3-48 cM

Exon count:: 2

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (7909598..7911779, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (3735320..3737501, complement)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

DREF binds directly upstream of Prat and the DRE sites associated with its activity are necessary for Prat expression
Title: Transcriptional regulation of the purine de novo synthesis gene Prat in Drosophila melanogaster.
Data show the presence of apoptosis at the cellular level in both ade2 and Prat mutants, and the upregulated gene HtrA2, which encodes an apoptosis effector and is thus a candidate for initiating apoptosis in response to purine depletion.
Title: A link between impaired purine nucleotide synthesis and apoptosis in Drosophila melanogaster.
whereas Mod(Prat:bw)3-5 suppresses Prat in all stages and tissues examined for both sexes.
Title: Modifiers of Prat, a de novo purine synthesis gene, in Drosophila melanogaster.
Use of Prat and Prat2 double RNA interference causes more severe pupal lethal phenotypes and suggests that Prat and Prat2 have partially additive functions during Drosophila metamorphosis.
Title: The purine synthesis gene Prat2 is required for Drosophila metamorphosis, as revealed by inverted-repeat-mediated RNA interference.
Data show that the requirement for both Prat and Prat2 in Drosophila is not simply due to differences in protein function. Both genes have developed nonoverlapping patterns of expression, which correlate with their respective loss-of-function phenotypes.
Title: Expression pattern diversity and functional conservation between retroposed PRAT genes from Drosophila melanogaster and Drosophila virilis.
Regulation of expression of prat in the adult eye was studied.
Title: Identification of trans-dominant modifiers of Prat expression in Drosophila melanogaster.
Evidence suggests that Prat originated by gene duplication by retrotransposition of Prat2.
Title: The PRAT purine synthesis gene duplication in Drosophila melanogaster and Drosophila virilis is associated with a retrotransposition event and diversification of expression patterns.
limiting factor in the processes of cellular or nuclear proliferation that take place during egg chamber and embryonic development
Title: Drosophila melanogaster Prat, a purine de novo synthesis gene, has a pleiotropic maternal-effect phenotype.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables amidophosphoribosyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables amidophosphoribosyltransferase activity	IEA Inferred from Electronic Annotation more info
enables amidophosphoribosyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in determination of adult lifespan	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in embryonic cleavage	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in purine nucleobase biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in purine nucleotide biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in purine nucleotide biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed

General protein information

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Preferred Names: phosphoribosylamidotransferase

Names: CG2867-PA; CG2867-PB; Prat-PA; Prat-PB; transcript D

NP_001246972.1

EC 2.4.2.14

NP_524271.2

EC 2.4.2.14

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

7909598..7911779 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_079547.3 → NP_524271.2 phosphoribosylamidotransferase, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_524271.2

Status: REVIEWED

UniProtKB/Swiss-Prot

Q27601, Q8T009, Q9VHY4

UniProtKB/TrEMBL

A0A0B4K6L4, A0APG2

Related

FBpp0081261

Conserved Domains (3) summary

cd06223
Location:347 → 483

PRTases_typeI; Phosphoribosyl transferase (PRT)-type I domain

COG0034
Location:50 → 543

PurF; Glutamine phosphoribosylpyrophosphate amidotransferase [Nucleotide transport and metabolism]

cd00715
Location:54 → 336

GPATase_N; Glutamine amidotransferases class-II (GN-AT)_GPAT- type. This domain is found at the N-terminus of glutamine phosphoribosylpyrophosphate (Prpp) amidotransferase (GPATase) . The glutaminase domain catalyzes amide nitrogen transfer from glutamine to the ...
NM_001260043.1 → NP_001246972.1 phosphoribosylamidotransferase, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001246972.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q27601, Q8T009, Q9VHY4

UniProtKB/TrEMBL

A0A0B4K6L4, A0APG2

Conserved Domains (3) summary

cd06223
Location:347 → 483

PRTases_typeI; Phosphoribosyl transferase (PRT)-type I domain

COG0034
Location:50 → 543

PurF; Glutamine phosphoribosylpyrophosphate amidotransferase [Nucleotide transport and metabolism]

cd00715
Location:54 → 336

GPATase_N; Glutamine amidotransferases class-II (GN-AT)_GPAT- type. This domain is found at the N-terminus of glutamine phosphoribosylpyrophosphate (Prpp) amidotransferase (GPATase) . The glutaminase domain catalyzes amide nitrogen transfer from glutamine to the ...