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    LIMK2 LIM domain kinase 2 [ Homo sapiens ]

    Gene ID: 3985, updated on 19-May-2012

    Summary

    Official Symbol
    LIMK2provided by HGNC
    Official Full Name
    LIM domain kinase 2provided by HGNC
    Primary source
    HGNC:6614
    See related
    Ensembl:ENSG00000182541; HPRD:03585; MIM:601988; Vega:OTTHUMG00000151251
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Summary
    There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

    Genomic context

    Location :
    22q12.2
    Sequence :
    Chromosome: 22; NC_000022.10 (31608250..31676066)
    See LIMK2 in Epigenomics, MapViewer

    Chromosome 22 - NC_000022.10Genomic Context describing neighboring genes Neighboring gene microRNA 3928 Neighboring gene ring finger protein 185 Neighboring gene protein phosphatase 1, regulatory (inhibitor) subunit 14B pseudogene 1 Neighboring gene phosphoinositide-3-kinase interacting protein 1 Neighboring gene uncharacterized protein MGC15705 Neighboring gene POZ (BTB) and AT hook containing zinc finger 1

    Genomic regions, transcripts, and products

    Bibliography

    GeneRIFs: Gene References Into Functions What's a GeneRIF?

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description
    P53671 Q5VT25 CDC42BPA    HPRD  PubMed  
    P53671 P23528 CFL1    HPRD  PubMed  
    P53671 P06396 GSN    HPRD  PubMed  
    P53671 Q9H422 HIPK3    HPRD  PubMed  
    P53671 P53667 LIMK1    HPRD  PubMed  
    P53671 Q8TEW0 PARD3    HPRD  PubMed  
    P53671 Q13464 ROCK1    HPRD  PubMed  
    BioGRID:110173 BioGRID:107499 CFL1    BioGRID  PubMed Affinity Capture-Western 
    BioGRID:110173 BioGRID:110172 LIMK1    BioGRID  PubMed Affinity Capture-Western 
    BioGRID:110173 BioGRID:112020 ROCK1    BioGRID  PubMed Biochemical Activity 
    NP_061857.2 SSH1    BIND  PubMed SSH-1L interacts with and dephosphorylates an unspecified isoform of LIMK2. 

    General gene information

    Markers

    Homology

    Pathways from BioSystems

    • Axon guidance, organism-specific biosystem (from KEGG)
      Axon guidance, organism-specific biosystemAxon guidance represents a key stage in the formation of neuronal network. Axons are guided by a variety of guidance factors, such as netrins, ephrins, Slits, and semaphorins. These guidance cues are...
    • Axon guidance, conserved biosystem (from KEGG)
      Axon guidance, conserved biosystemAxon guidance represents a key stage in the formation of neuronal network. Axons are guided by a variety of guidance factors, such as netrins, ephrins, Slits, and semaphorins. These guidance cues are...
    • Axon guidance, organism-specific biosystem (from REACTOME)
      Axon guidance, organism-specific biosystemAxon guidance / axon pathfinding is the process by which neurons send out axons to reach the correct targets. Growing axons have a highly motile structure at the growing tip called the growth cone, w...
    • CDC42 signaling events, organism-specific biosystem (from Pathway Interaction Database)
      CDC42 signaling events, organism-specific biosystem
      CDC42 signaling events
    • Developmental Biology, organism-specific biosystem (from REACTOME)
      Developmental Biology, organism-specific biosystemAs a first step towards capturing the array of processes by which a fertilized egg gives rise to the diverse tissues of the body, examples of three kinds of processes have been annotated. These are a...
    • Fc gamma R-mediated phagocytosis, organism-specific biosystem (from KEGG)
      Fc gamma R-mediated phagocytosis, organism-specific biosystemPhagocytosis plays an essential role in host-defense mechanisms through the uptake and destruction of infectious pathogens. Specialized cell types including macrophages, neutrophils, and monocytes ta...
    • Fc gamma R-mediated phagocytosis, conserved biosystem (from KEGG)
      Fc gamma R-mediated phagocytosis, conserved biosystemPhagocytosis plays an essential role in host-defense mechanisms through the uptake and destruction of infectious pathogens. Specialized cell types including macrophages, neutrophils, and monocytes ta...
    • Regulation of actin cytoskeleton, organism-specific biosystem (from KEGG)
      Regulation of actin cytoskeleton, organism-specific biosystem
      Regulation of actin cytoskeleton
    • Regulation of actin cytoskeleton, conserved biosystem (from KEGG)
      Regulation of actin cytoskeleton, conserved biosystem
      Regulation of actin cytoskeleton
    • RhoA signaling pathway, organism-specific biosystem (from Pathway Interaction Database)
      RhoA signaling pathway, organism-specific biosystem
      RhoA signaling pathway
    • Sema4D in semaphorin signaling, organism-specific biosystem (from REACTOME)
      Sema4D in semaphorin signaling, organism-specific biosystemSemaphorin 4D (Sema 4D/CD100) is an axon guidance molecule with two disulfide-linked 150-kDa subunits. SEMA4D is structurally defined by a conserved 500-amino acid extracellular domain with 16 cyste...
    • Sema4D induced cell migration and growth-cone collapse, organism-specific biosystem (from REACTOME)
      Sema4D induced cell migration and growth-cone collapse, organism-specific biosystemSema4D-mediated attraction of endothelial cells requires Rho, but not R-Ras, signaling. Sema4D-mediated plexinB1 activation activates Rho and its downstream effector ROCK. ROCK then phosphorylates ML...
    • Semaphorin interactions, organism-specific biosystem (from REACTOME)
      Semaphorin interactions, organism-specific biosystemSemaphorins are a large family of cell surface and secreted guidance molecules divided into eight classes on the basis of their structures. They all have an N-terminal conserved sema domain. Semaphor...

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    ATP binding IEA
    Inferred from Electronic Annotation
    more info
     
    metal ion binding IEA
    Inferred from Electronic Annotation
    more info
     
    nucleotide binding IEA
    Inferred from Electronic Annotation
    more info
     
    protein heterodimerization activity IEA
    Inferred from Electronic Annotation
    more info
     
    protein serine/threonine kinase activity IEA
    Inferred from Electronic Annotation
    more info
     
    protein serine/threonine kinase activity TAS
    Traceable Author Statement
    more info
    PubMed 
    zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
     
    Process Evidence Code Pubs
    phosphorylation TAS
    Traceable Author Statement
    more info
    PubMed 
    regulation of phosphorylation IEA
    Inferred from Electronic Annotation
    more info
     
    spermatogenesis IEA
    Inferred from Electronic Annotation
    more info
     
    Component Evidence Code Pubs
    cis-Golgi network IEA
    Inferred from Electronic Annotation
    more info
     
    cytoplasm IDA
    Inferred from Direct Assay
    more info
    PubMed 
    cytoplasm TAS
    Traceable Author Statement
    more info
    PubMed 
    mitochondrion IDA
    Inferred from Direct Assay
    more info
    PubMed 
    nucleus IEA
    Inferred from Electronic Annotation
    more info
     
    nucleus TAS
    Traceable Author Statement
    more info
    PubMed 
    soluble fraction IEA
    Inferred from Electronic Annotation
    more info
     

    General protein information

    Preferred Names
    LIM domain kinase 2
    Names
    LIM domain kinase 2
    NP_001026971.1
    NP_005560.1
    NP_057952.1

    NCBI Reference Sequences (RefSeq)

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    Genomic

    1. NG_029895.1 RefSeqGene

      Range
      5001..72817
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001031801.1NP_001026971.1  LIM domain kinase 2 isoform 1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) encodes the longest isoform (1).
      Source sequence(s)
      BC013051, BU553494
      Consensus CDS
      CCDS33637.1
      Related
      ENSP00000339916, OTTHUMP00000199484, ENST00000340552, OTTHUMT00000321913
      Conserved Domains (6) summary
      cd00992
      Location:129215
      Blast Score: 125
      PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...
      pfam05361
      Location:590685
      Blast Score: 280
      PP1_inhibitor; PKC-activated protein phosphatase-1 inhibitor
      cd00180
      Location:316518
      Blast Score: 465
      PKc; Catalytic domain of Protein Kinases
      cd09465
      Location:46104
      Blast Score: 345
      LIM2_LIMK2; The second LIM domain of LIMK2 (LIM domain Kinase 2)
      smart00221
      Location:313565
      Blast Score: 468
      STYKc; Protein kinase; unclassified specificity.
      cl02475
      Location:1544
      Blast Score: 180
      LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers.
    2. NM_005569.3NP_005560.1  LIM domain kinase 2 isoform 2a

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2a) differs in the 5' UTR and coding region as well as the 3' UTR and coding region, compared to variant 1. The resulting isoform (2a) is shorter and has distinct N- and C-termini compared to isoform 1.
      Source sequence(s)
      AC002073, BC013051, BX385507, D45906
      Consensus CDS
      CCDS13891.1
      UniProtKB/Swiss-Prot
      P53671
      Related
      ENSP00000332687, OTTHUMP00000199482, ENST00000331728, OTTHUMT00000321911
      Conserved Domains (5) summary
      cd00992
      Location:150236
      Blast Score: 126
      PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...
      cd09463
      Location:1265
      Blast Score: 301
      LIM1_LIMK2; The first LIM domain of LIMK2 (LIM domain Kinase 2)
      cd09465
      Location:67125
      Blast Score: 341
      LIM2_LIMK2; The second LIM domain of LIMK2 (LIM domain Kinase 2)
      smart00221
      Location:334601
      Blast Score: 519
      STYKc; Protein kinase; unclassified specificity.
      cl09925
      Location:335602
      Blast Score: 504
      PKc_like; Protein Kinases, catalytic domain
    3. NM_016733.2NP_057952.1  LIM domain kinase 2 isoform 2b

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2b) differs in the 3' UTR and coding region compared to variant 1. The resulting isoform (2b) is shorter and has a distinct C-terminus compared to isoform 1.
      Source sequence(s)
      AC002073, BC013051, BU553494
      Consensus CDS
      CCDS13892.1
      UniProtKB/Swiss-Prot
      P53671
      Related
      ENSP00000330470, OTTHUMP00000199485, ENST00000333611, OTTHUMT00000321914
      Conserved Domains (5) summary
      cd00992
      Location:129215
      Blast Score: 126
      PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...
      cd09465
      Location:46104
      Blast Score: 343
      LIM2_LIMK2; The second LIM domain of LIMK2 (LIM domain Kinase 2)
      smart00221
      Location:313580
      Blast Score: 519
      STYKc; Protein kinase; unclassified specificity.
      cl02475
      Location:1544
      Blast Score: 182
      LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers.
      cl09925
      Location:314581
      Blast Score: 503
      PKc_like; Protein Kinases, catalytic domain

    RefSeqs of Annotated Genomes: Build 37.3

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh37.p5 Primary Assembly

    Genomic

    1. NC_000022.10 Reference GRCh37.p5 Primary Assembly

      Range
      31608250..31676066
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate HuRef

    Genomic

    1. AC_000154.1 Alternate HuRef

      Range
      14569980..14637678
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

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