LIMK1 LIM domain kinase 1 [Homo sapiens] - Gene

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Summary

Official Symbol: LIMK1provided by HGNC
Official Full Name: LIM domain kinase 1provided by HGNC
Primary source: HGNC:6613
See related: Ensembl:ENSG00000106683; HPRD:03210; MIM:601329; Vega:OTTHUMG00000023448
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: LIMK; LIMK-1
Summary: There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Feb 2011]

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Genomic context

Location :: 7q11.23

Sequence :: Chromosome: 7; NC_000007.13 (73498107..73536855)

See LIMK1 in Epigenomics, MapViewer

Chromosome 7 - NC_000007.13 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data describe the role of LIMK1-mediated CFL1 pathway and actin dynamics in retinoid receptor mediated function and show that LIMK1-mediated phosphocycling of CFL1 plays a role in maintaining actin homeostasis and receptor activity.
Title: LIM kinase 1 - dependent cofilin 1 pathway and actin dynamics mediate nuclear retinoid receptor function in T lymphocytes.
LIMK1 has a dual role in regulating lamellipodium extension by decelerating actin retrograde flow and polymerization.
Title: LIM kinase has a dual role in regulating lamellipodium extension by decelerating the rate of actin retrograde flow and the rate of actin polymerization.
LIMK1 activity in both the cytoplasmic and nuclear compartments promotes breast cancer progression.
Title: Nuclear and cytoplasmic LIMK1 enhances human breast cancer progression.
LIM kinase 1 modulates cortical actin and CXCR4 cycling and is activated by HIV-1 to initiate viral infection.
Title: LIM kinase 1 modulates cortical actin and CXCR4 cycling and is activated by HIV-1 to initiate viral infection.
evealed a SNP, rs6460071 located on LIMK1 gene (P = 0.00069) to be significantly associated with increased risk of intracranial aneurysm
Title: Impact of LIMK1, MMP2 and TNF-α variations for intracranial aneurysm in Japanese population.
LIM kinase1 modulates function of membrane type matrix metalloproteinase 1 in prostate cancer cells
Title: LIM kinase1 modulates function of membrane type matrix metalloproteinase 1: implication in invasion of prostate cancer cells.
Changes in the expression of cytoskeletal regulatory proteins such as LIMK and cofilin may play a role in weakening thoracic aortic medial tissue, as a precondition to thoracic aortic dissection.
Title: A study of the expression and interaction of Destrin, cofilin, and LIMK in Debakey I type thoracic aortic dissection tissue.
High LIMK1 is associated with cervical cancer dysplasia.
Title: Expression profiling of G2/M phase regulatory proteins in normal, premalignant and malignant uterine cervix and their correlation with survival of patients.
FXa induced myosin light chain phosphorylation and LIMK1 activation resulting in cofilin inactivation
Title: Coagulation Factor Xa inhibits cancer cell migration via LIMK1-mediated cofilin inactivation.
phosphorylation of annexin 1 regulates the angiogenic effect that is associated with the activation of the p38/LIM kinase 1 axis by VEGF
Title: Regulation of vascular endothelial growth factor-induced endothelial cell migration by LIM kinase 1-mediated phosphorylation of annexin 1.

Submit: New GeneRIF Correction See all (41)

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Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

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Products	Interactant	Other Gene	Source	Pubs	Description
NP_002305.1	NP_004681.1	LATS1	BIND	PubMed	LATS1 interacts with LIMK1.
NP_002305.1	NP_005875.1	PAK4	BIND	PubMed	PAK4 interacts with and phosphorylates LIMK1.
NP_002305.1	BAB84115.1	SSH1	BIND	PubMed	SSH-1S interacts with LIMK1.
NP_002305.1	NP_061857.2	SSH1	BIND	PubMed	SSH-1L interacts with and dephosphorylates LIMK1.
NP_002305.1		SSH2	BIND	PubMed	The shorter isoform, SSH-2S, interacts with LIMK1.
NP_002305.1	NP_060746.2	SSH3	BIND	PubMed	SSH-3S interacts with LIMK1.
P53667	O95477	ABCA1	HPRD	PubMed
P53667	Q13873	BMPR2	HPRD	PubMed
P53667	Q5VT25	CDC42BPA	HPRD	PubMed
P53667	P49918	CDKN1C	HPRD	PubMed
P53667	P23528	CFL1	HPRD	PubMed
P53667	Q9Y281	CFL2	HPRD	PubMed
P53667	O95835	LATS1	HPRD	PubMed
P53667	P53667	LIMK1	HPRD	PubMed
P53667	P53667	LIMK1	HPRD	PubMed
P53667	P53671	LIMK2	HPRD	PubMed
P53667	Q02297	NRG1	HPRD	PubMed
P53667	Q13153	PAK1	HPRD	PubMed
P53667	Q13177	PAK2	HPRD	PubMed
P53667	O96013	PAK4	HPRD	PubMed
P53667	P49023	PXN	HPRD	PubMed
P53667	Q9NVW2	RLIM	HPRD	PubMed
P53667	Q13464	ROCK1	HPRD	PubMed
P53667	Q8TE77	SSH3	HPRD	PubMed
P53667	P63104	YWHAZ	HPRD	PubMed
BioGRID:110172	BioGRID:107127	BMPR2	BioGRID	PubMed	Affinity Capture-Western; Two-hybrid
BioGRID:110172	BioGRID:107462	CDKN1C	BioGRID	PubMed	Affinity Capture-Western; Two-hybrid
BioGRID:110172	BioGRID:107499	CFL1	BioGRID	PubMed	Biochemical Activity
BioGRID:110172	BioGRID:109540	HSPA4	BioGRID	PubMed	Affinity Capture-Western
BioGRID:110172	BioGRID:110172	LIMK1	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex
BioGRID:110172	BioGRID:110173	LIMK2	BioGRID	PubMed	Affinity Capture-Western
BioGRID:110172	BioGRID:109332	NRG1	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex; Two-hybrid
BioGRID:110172	BioGRID:111095	PAK1	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex
BioGRID:110172	BioGRID:115586	PAK4	BioGRID	PubMed	Biochemical Activity; Reconstituted Complex
BioGRID:110172	BioGRID:111105	PARK2	BioGRID	PubMed	Affinity Capture-Western
BioGRID:110172	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-Western
BioGRID:110172	BioGRID:113366	YWHAZ	BioGRID	PubMed	Reconstituted Complex; Two-hybrid

Function	Evidence Code	Pubs
ATP binding	IEA Inferred from Electronic Annotation more info
heat shock protein binding	IDA Inferred from Direct Assay more info	PubMed
metal ion binding	IEA Inferred from Electronic Annotation more info
nucleotide binding	IEA Inferred from Electronic Annotation more info
protein binding	IPI Inferred from Physical Interaction more info	PubMed
protein kinase activity	NAS Non-traceable Author Statement more info	PubMed
protein serine/threonine kinase activity	TAS Traceable Author Statement more info
zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
Rho protein signal transduction	TAS Traceable Author Statement more info	PubMed
actin cytoskeleton organization	TAS Traceable Author Statement more info	PubMed
axon guidance	TAS Traceable Author Statement more info
negative regulation of ubiquitin-protein ligase activity	IDA Inferred from Direct Assay more info	PubMed
nervous system development	TAS Traceable Author Statement more info	PubMed
positive regulation of actin filament bundle assembly	IDA Inferred from Direct Assay more info	PubMed
positive regulation of axon extension	ISS Inferred from Sequence or Structural Similarity more info
protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
protein phosphorylation	NAS Non-traceable Author Statement more info	PubMed
signal transduction	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
cytoplasm	IDA Inferred from Direct Assay more info	PubMed
cytosol	TAS Traceable Author Statement more info
neuron projection	ISS Inferred from Sequence or Structural Similarity more info
NOT nucleolus	IDA Inferred from Direct Assay more info	PubMed
nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

Preferred Names: LIM domain kinase 1

Names: LIM domain kinase 1; LIM motif-containing protein kinase

NP_001191355.1

EC 2.7.11.1

NP_002305.1

EC 2.7.11.1

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NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_008129.1 RefSeqGene

Range

4952..43700

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001204426.1 → NP_001191355.1 LIM domain kinase 1 isoform 2

Status: REVIEWED

Description

Transcript Variant: This variant (2) lacks multiple exons at the 5' end and uses a different start codon, compared to variant 1. This variant encodes an isoform (2) with a shorter and distinct N-terminus that lacks one of two LIM zinc-binding domains, compared to isoform 1.

Source sequence(s)

AK300382, AW016257, D26309, DC296332

Consensus CDS

CCDS56491.1

UniProtKB/Swiss-Prot

P53667

Related

ENSP00000444452, ENST00000538333

Conserved Domains (5) summary

cd00992
Location:129 – 221
Blast Score: 128

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

cd00180
Location:311 – 563
Blast Score: 487

PKc; Catalytic domain of Protein Kinases

cd09464
Location:50 – 104
Blast Score: 306

LIM2_LIMK1; The second LIM domain of LIMK1 (LIM domain Kinase 1)

smart00219
Location:308 – 570
Blast Score: 539

TyrKc; Tyrosine kinase, catalytic domain

cl02475
Location:17 – 43
Blast Score: 164

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers.
NM_002314.3 → NP_002305.1 LIM domain kinase 1 isoform 1

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AC005056, AW016257, D26309

Consensus CDS

CCDS5563.1

UniProtKB/Swiss-Prot

P53667

Related

ENSP00000336740, OTTHUMP00000160532, ENST00000336180, OTTHUMT00000252335

Conserved Domains (5) summary

cd00992
Location:163 – 255
Blast Score: 130

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

cd00180
Location:345 – 597
Blast Score: 488

PKc; Catalytic domain of Protein Kinases

cd09462
Location:5 – 77
Blast Score: 415

LIM1_LIMK1; The first LIM domain of LIMK1 (LIM domain Kinase 1)

smart00219
Location:342 – 604
Blast Score: 538

TyrKc; Tyrosine kinase, catalytic domain

cl02475
Location:84 – 138
Blast Score: 300

LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers.

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000007.13 Reference GRCh37.p5 Primary Assembly

Range

73498107..73536855

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000139.1 Alternate HuRef

Range

69382814..69418976

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate CRA_TCAGchr7v2

Genomic

AC_000068.1 Alternate CRA_TCAGchr7v2

Range

72831179..72869927

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

NM_016735.1: Suppressed sequence

Description

NM_016735.1: This RefSeq was permanently suppressed because it is a nonsense-mediated mRNA decay (NMD) candidate.

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Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AC005056.2	AAS07436.1
genomic	AC005057.2	AAS07437.1
		AAS07438.1
genomic	CH471200.2	EAW69620.1
		EAW69621.1
		EAW69622.1
		EAW69623.1
genomic	U62293.1	AAB17545.1
		AAB17546.1
genomic	U63721.1	AAC13885.1
		AAC13886.1
mRNA	AB209557.1	BAD92794.1
mRNA	AF134379.1	AAD25742.1
mRNA	AK026544.1	None
mRNA	AK125156.1	None
mRNA	AK290162.1	BAF82851.1
mRNA	AK300382.1	BAH13274.1
mRNA	AW016257.1	None
mRNA	D26309.1	BAA05371.1
mRNA	DC296332.1	None
other-genetic	BC148340.1	AAI48341.1
other-genetic	BC152982.1	AAI52983.1

Protein Accession	Links
Protein Accession	GenPept Link	UniProtKB Link
P53667.3	GenPept	UniProtKB/Swiss-Prot:P53667
Q59FA3	GenPept	UniProtKB/TrEMBL:Q59FA3
Q75MU0	GenPept	UniProtKB/TrEMBL:Q75MU0
Q75MU4	GenPept	UniProtKB/TrEMBL:Q75MU4