tpx2.L TPX2, microtubule-associated L homeolog [Xenopus laevis (African clawed frog)] - Gene

Summary

Official Symbol: tpx2.L
Official Full Name: TPX2, microtubule-associated L homeolog
Primary source: Xenbase:XB-GENE-6251919
Locus tag: XELAEV_18043657mg
See related: EnsemblRapid:ENSXLAG00005030564
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Xenopus laevis
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia; Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus
Also known as: dil2; tpx2; dil-2; hctp4; repp86; tpx2-a; tpx2-b
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Genomic context

Location:: chromosome: 9_10L

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
101	current	Xenopus_laevis_v10.1 (GCF_017654675.1)	9_10L	NC_054387.1 (19003195..19019751, complement)
100	previous assembly	Xenopus_laevis_v2 (GCF_001663975.1)	9_10L	NC_030740.1 (18643842..18660421, complement)

Chromosome 9_10L - NC_054387.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Biochemical reconstitution of branching microtubule nucleation.
Title: Biochemical reconstitution of branching microtubule nucleation.
By regulating the availability of the branching effectors TPX2, augmin and gamma-TuRC, combined with single-molecule observations, we show that first TPX2 is deposited on pre-existing microtubules (MTs), followed by binding of augmin/gamma-TuRC to result in the nucleation of branched MTs.
Title: Spatiotemporal organization of branched microtubule networks.
This study identifies TPX2 as the only direct RanGTP target and NEDD1 as the only Aurora A substrate essential for the activation of the RanGTP-dependent microtubule nucleation pathway.
Title: Microtubule nucleation in mitosis by a RanGTP-dependent protein complex.
TPX2 levels modulate spindle architecture through Eg5 in Xenopus oocyte extract.
Title: TPX2 levels modulate meiotic spindle size and architecture in Xenopus egg extracts.
analysis of binding of the mitotic regulator TPX2 (target protein for Xenopus kinesin-like protein 2) to importin-alpha
Title: Novel binding of the mitotic regulator TPX2 (target protein for Xenopus kinesin-like protein 2) to importin-alpha.
Plx1 promotes activation of Aurora A, most likely through TPX2.
Title: Phosphorylation of TPX2 by Plx1 enhances activation of Aurora A.
Data show that depletion of TPX2 was partially rescued by the addition of excess XMAP215 or EB1, or inhibiting MCAK (a Kinesin-13).
Title: Functional overlap of microtubule assembly factors in chromatin-promoted spindle assembly.
HURP and TPX2 have nonredundant functions essential for chromatin-induced microtubule assembly.
Title: Hepatoma up-regulated protein is required for chromatin-induced microtubule assembly independently of TPX2.
TPX2 interacts with lamina-associated polypeptide 2 (LAP2). LAP2 localization is disrupted in TPX2-depleted nuclei, suggesting interaction between TPX2 and LAP2 is required for postmitotic nuclear reformation
Title: TPX2 is required for postmitotic nuclear assembly in cell-free Xenopus laevis egg extracts.
Here we identify G205 in Xenopus Aurora A as a key determinant of both intrinsic activity and regulation by TPX2.
Title: The Aurora A and Aurora B protein kinases: a single amino acid difference controls intrinsic activity and activation by TPX2.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by Xenbase

Function	Evidence Code	Pubs
enables microtubule binding	IEA Inferred from Electronic Annotation more info
enables protein kinase activator activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within cell cycle	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cell division	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within mitotic spindle assembly	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of mitotic spindle organization	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in microtubule	IEA Inferred from Electronic Annotation more info
located_in nuclear microtubule	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in spindle	IEA Inferred from Electronic Annotation more info
located_in spindle pole	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: targeting protein for Xklp2-A

Names: TPX2 protein, microtubule-associated; targeting protein for Xklp2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001088537.1 → NP_001082006.1 targeting protein for Xklp2-A

See identical proteins and their annotated locations for NP_001082006.1

Status: PROVISIONAL

Source sequence(s)

BC068637

UniProtKB/Swiss-Prot

Q6NUF4, Q9I8N0

UniProtKB/TrEMBL

A0A974BX77

Conserved Domains (3) summary

pfam06886
Location:633 → 687

TPX2; Targeting protein for Xklp2 (TPX2)

pfam09041
Location:1 → 64

Aurora-A_bind; Aurora-A binding

pfam12214
Location:331 → 458

TPX2_importin; Cell cycle regulated microtubule associated protein

RefSeqs of Annotated Genomes: Xenopus laevis Annotation Release 101 details...

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference Xenopus_laevis_v10.1 Primary Assembly

Genomic

NC_054387.1 Reference Xenopus_laevis_v10.1 Primary Assembly

Range

19003195..19019751 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)