ARHGDIA Rho GDP dissociation inhibitor (GDI) alpha [Homo sapiens (human)] - Gene

Summary

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Official Symbol: ARHGDIAprovided by HGNC
Official Full Name: Rho GDP dissociation inhibitor (GDI) alphaprovided by HGNC
Primary source: HGNC:678
See related: Ensembl:ENSG00000141522; HPRD:03565; MIM:601925; Vega:OTTHUMG00000178352
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: GDIA1; RHOGDI; RHOGDI-1
Summary: Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA (Leffers et al., 1993 [PubMed 8262133]).[supplied by OMIM, Jan 2009]

Genomic context

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Location :: 17q25.3

Sequence :: Chromosome: 17; NC_000017.10 (79825597..79829282, complement)

See ARHGDIA in Epigenomics, MapViewer

Chromosome 17 - NC_000017.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence

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Bibliography

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GeneRIFs: Gene References Into Functions What's a GeneRIF?

demonstrate that membrane extraction of Rho GTPase by RhoGDI is a thermodynamically favored passive process that operates through a series of progressively tighter intermediates, much like the one that is mediated by RabGDI
Title: Quantitative analysis of prenylated RhoA interaction with its chaperone, RhoGDI.
Knockdown of RhoGDIalpha induces apoptosis and increases lung cancer cell chemosensitivity to paclitaxel.
Title: Knockdown of RhoGDIα induces apoptosis and increases lung cancer cell chemosensitivity to paclitaxel.
The physiological regulation of RhoGDI SUMOylation by the RING domain of XIAP may account for modulation of cancer cell invasion and metastasis by XIAP
Title: RhoGDI SUMOylation at Lys-138 increases its binding activity to Rho GTPase and its inhibiting cancer cell motility.
Rho GDI may be useful as a diagnostic biomarker and/or a therapeutic to prevent colon and prostate cancer metastasis
Title: Rho GDP-dissociation inhibitor alpha is associated with cancer metastasis in colon and prostate cancer.
Loss of GDIalpha expression promotes the development and progression of prostate cancer.
Title: RhoGDIα suppresses growth and survival of prostate cancer cells.
Both the mRNA and protein expressions of Rho-GDI in the decidual tissues were significantly higher in the normal pregnancy group than in the two severe preeclampsia groups.
Title: [Expression of Rho-GDP dissociation inhibitor in the decidual tissues of preeclampsia patients and its clinical implication].
Data show that knockdown of S100P led to downregulation of thioredoxin 1 and beta-tubulin and upregulation of RhoGDIA, all potential therapeutic targets in cancer.
Title: Targeting S100P inhibits colon cancer growth and metastasis by Lentivirus-mediated RNA interference and proteomic analysis.
RhoGDIalpha-dependent balance between RhoA and RhoC is a key regulator of cancer cell tumorigenesis
Title: RhoGDIα-dependent balance between RhoA and RhoC is a key regulator of cancer cell tumorigenesis.
The RhoGDIalpha protein is located in the acrosome and flagellum of human sperm, and might be involved in sperm movement, capacitation and acrosome reaction.
Title: [Expression of RhoGDI alpha in human testes and sperm and its correlation with the success rate of IVF].
We provide evidence that ARHGDIA, COBLL1, and TM4SF1 are negative regulators of apoptosis in cultured tumor cells.
Title: Genes associated with prognosis after surgery for malignant pleural mesothelioma promote tumor cell survival in vitro.

Submit: New GeneRIF Correction See all (33)

Phenotypes

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Review eQTL and phenotype association data in this region using PheGenI

HIV-1 protein interactions

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Protein	Gene	Interaction	Pubs
Tat, p14	tat	In Jurkat cells expressing HIV-1 Tat, decreased expression levels are found for basic cytoskeletal proteins such as actin, beta-tubulin, annexin, cofilin, gelsolin, and Rac/Rho-GDI complex	PubMed
Vpr, p15	vpr	HIV-1 Vpr-expressing Jurkat T cell clones showed a significant upregulation of Rho GDI expression	PubMed

Go to the HIV-1, Human Protein Interaction Database

Interactions

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Products	Interactant	Other Gene	Source	Pubs	Description
NP_004300.1	NP_002567.3	PAK1	BIND	PubMed	Pak1 interacts with and phosphorylates RhoGDI. This interaction was modeled on a demonstrated interaction between Pak1 from an unspecified species and human RhoGDI.
P52565	O96019	ACTL6A	HPRD	PubMed
P52565	P52565	ARHGDIA	HPRD	PubMed
P52565	P60953	CDC42	HPRD	PubMed
P52565	P46527	CDKN1B	HPRD	PubMed
P52565	P39748	FEN1	HPRD	PubMed
P52565	P14923	JUP	HPRD	PubMed
P52565	Q96PU5	NEDD4L	HPRD	PubMed
P52565	P63000	RAC1	HPRD	PubMed
P52565	P15153	RAC2	HPRD	PubMed
P52565	P35241	RDX	HPRD	PubMed
P52565	P61586	RHOA	HPRD	PubMed
P52565	Q99963	SH3GL3	HPRD	PubMed
P52565	Q11206	ST3GAL4	HPRD	PubMed
P52565	Q6EEV6	SUMO4	HPRD	PubMed
P52565	P15498	VAV1	HPRD	PubMed
BioGRID:106889	BioGRID:106601	ACTL6A	BioGRID	PubMed	Two-hybrid
BioGRID:106889	BioGRID:106889	ARHGDIA	BioGRID	PubMed	Reconstituted Complex
BioGRID:106889	BioGRID:110433	ATXN3	BioGRID	PubMed	Two-hybrid
BioGRID:106889	BioGRID:107054	BAK1	BioGRID	PubMed	Two-hybrid
BioGRID:106889	BioGRID:107106	BID	BioGRID	PubMed	Co-purification
BioGRID:106889	BioGRID:107293	CASP10	BioGRID	PubMed	Co-purification
BioGRID:106889	BioGRID:107291	CASP8	BioGRID	PubMed	Co-purification
BioGRID:106889	BioGRID:117030	CBX5	BioGRID	PubMed	Two-hybrid
BioGRID:106889	BioGRID:107433	CDC42	BioGRID	PubMed	Affinity Capture-MS; Co-purification; Two-hybrid
BioGRID:106889	BioGRID:107452	CDK2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:106889	BioGRID:107461	CDKN1B	BioGRID	PubMed	Two-hybrid
BioGRID:106889	BioGRID:114030	CUL3	BioGRID	PubMed	Affinity Capture-MS
BioGRID:106889	BioGRID:108025	DHX9	BioGRID	PubMed	Affinity Capture-MS
BioGRID:106889	BioGRID:108286	EIF2B1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:106889	BioGRID:108403	ESR1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:106889	BioGRID:108431	EWSR1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:106889	BioGRID:113271	EZR	BioGRID	PubMed	Co-purification
BioGRID:106889	BioGRID:114302	FADD	BioGRID	PubMed	Co-purification
BioGRID:106889	BioGRID:106851	FAS	BioGRID	PubMed	Co-purification
BioGRID:106889	BioGRID:106852	FASLG	BioGRID	PubMed	Co-purification
BioGRID:106889	BioGRID:108528	FEN1	BioGRID	PubMed	Two-hybrid
BioGRID:106889	BioGRID:117047	HAAO	BioGRID	PubMed	Two-hybrid
BioGRID:106889	BioGRID:115060	HERPUD1	BioGRID	PubMed	Two-hybrid
BioGRID:106889	BioGRID:128670	HNRNPA3	BioGRID	PubMed	Affinity Capture-MS
BioGRID:106889	BioGRID:109423	HNRNPAB	BioGRID	PubMed	Affinity Capture-MS
BioGRID:106889	BioGRID:115886	IGF2BP1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:106889	BioGRID:109931	JUP	BioGRID	PubMed	Two-hybrid
BioGRID:106889	BioGRID:111583	MAPK6	BioGRID	PubMed	Two-hybrid
BioGRID:106889	BioGRID:111585	MAPK8	BioGRID	PubMed	Co-purification
BioGRID:106889	BioGRID:110584	MSN	BioGRID	PubMed	Co-purification
BioGRID:106889	BioGRID:116915	NEDD4L	BioGRID	PubMed	Two-hybrid
BioGRID:106889	BioGRID:114114	PIR	BioGRID	PubMed	Co-fractionation
BioGRID:106889	BioGRID:117417	POT1	BioGRID	PubMed	Two-hybrid
BioGRID:106889	BioGRID:111688	PSMD10	BioGRID	PubMed	Affinity Capture-Western
BioGRID:106889	BioGRID:111817	RAC1	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western; Co-crystal Structure; Co-purification; Reconstituted Complex; Two-hybrid
BioGRID:106889	BioGRID:111818	RAC2	BioGRID	PubMed	Co-purification; Two-hybrid
BioGRID:106889	BioGRID:111819	RAC3	BioGRID	PubMed	Two-hybrid
BioGRID:106889	BioGRID:111894	RDX	BioGRID	PubMed	Reconstituted Complex
BioGRID:106889	BioGRID:106880	RHOA	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western; Co-purification; Two-hybrid
BioGRID:106889	BioGRID:106882	RHOC	BioGRID	PubMed	Affinity Capture-MS
BioGRID:106889	BioGRID:106884	RHOG	BioGRID	PubMed	Two-hybrid
BioGRID:106889	BioGRID:106892	RHOH	BioGRID	PubMed	Affinity Capture-Western; Two-hybrid
BioGRID:106889	BioGRID:112292	SDHC	BioGRID	PubMed	Two-hybrid
BioGRID:106889	BioGRID:112354	SH3GL3	BioGRID	PubMed	Two-hybrid
BioGRID:106889	BioGRID:119602	SIRT7	BioGRID	PubMed	Affinity Capture-MS
BioGRID:106889	BioGRID:113188	SUMO1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:106889	BioGRID:112497	SUMO2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:106889	BioGRID:132223	SUMO4	BioGRID	PubMed	Affinity Capture-MS
BioGRID:106889	BioGRID:112872	TERF1	BioGRID	PubMed	Two-hybrid
BioGRID:106889	BioGRID:117660	TINF2	BioGRID	PubMed	Two-hybrid
BioGRID:106889	BioGRID:114323	TNFRSF10B	BioGRID	PubMed	Co-purification
BioGRID:106889	BioGRID:112986	TNFRSF1A	BioGRID	PubMed	Co-purification
BioGRID:106889	BioGRID:115501	TRIM13	BioGRID	PubMed	Two-hybrid
BioGRID:106889	BioGRID:116472	TWF2	BioGRID	PubMed	Two-hybrid
BioGRID:106889	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western
BioGRID:106889	BioGRID:114672	UBE2L6	BioGRID	PubMed	Two-hybrid
BioGRID:106889	BioGRID:120266	UBE2R2	BioGRID	PubMed	Two-hybrid
BioGRID:106889	BioGRID:119616	UFM1	BioGRID	PubMed	Two-hybrid
BioGRID:106889	BioGRID:106828	XIAP	BioGRID	PubMed	Affinity Capture-Western

Pathways from BioSystems

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Axonal growth inhibition (RHOA activation), organism-specific biosystem (from REACTOME)
Axonal growth inhibition (RHOA activation), organism-specific biosystemp75NTR can also form a receptor complex with the Nogo receptor (NgR). Such complexes mediates axonal outgrowth inhibitory signals of MDGIs (myelin-derived growth-inhibitors), such as Nogo66, myelin-a...
Axonal growth stimulation, organism-specific biosystem (from REACTOME)
Axonal growth stimulation, organism-specific biosystemComplex formation between p75NTR and RHOA can leads to inhibition of RHOA activity and axonal growth.
CDC42 signaling events, organism-specific biosystem (from Pathway Interaction Database)
CDC42 signaling events, organism-specific biosystem
CDC42 signaling events
Neurotrophin signaling pathway, organism-specific biosystem (from KEGG)
Neurotrophin signaling pathway, organism-specific biosystemNeurotrophins are a family of trophic factors involved in differentiation and survival of neural cells. The neurotrophin family consists of nerve growth factor (NGF), brain derived neurotrophic facto...
Neurotrophin signaling pathway, conserved biosystem (from KEGG)
Neurotrophin signaling pathway, conserved biosystemNeurotrophins are a family of trophic factors involved in differentiation and survival of neural cells. The neurotrophin family consists of nerve growth factor (NGF), brain derived neurotrophic facto...
RAC1 signaling pathway, organism-specific biosystem (from Pathway Interaction Database)
RAC1 signaling pathway, organism-specific biosystem
RAC1 signaling pathway
Regulation of CDC42 activity, organism-specific biosystem (from Pathway Interaction Database)
Regulation of CDC42 activity, organism-specific biosystem
Regulation of CDC42 activity
Regulation of RAC1 activity, organism-specific biosystem (from Pathway Interaction Database)
Regulation of RAC1 activity, organism-specific biosystem
Regulation of RAC1 activity
Regulation of RhoA activity, organism-specific biosystem (from Pathway Interaction Database)
Regulation of RhoA activity, organism-specific biosystem
Regulation of RhoA activity
Rho GTPase cycle, organism-specific biosystem (from REACTOME)
Rho GTPase cycle, organism-specific biosystemThe cycling of Rho GTPases is tightly controlled by three classes of protein. These are (1) guanine nucleotide dissociation inhibitors or GDIs, which maintain Rho proteins in an inactive state in the...
Signal Transduction, organism-specific biosystem (from REACTOME)
Signal Transduction, organism-specific biosystemSignal transduction is a process in which extracellular signals elicit changes in cell state and activity. Transmembrane receptors sense changes in the cellular environment by binding ligands, such a...
Signaling by Rho GTPases, organism-specific biosystem (from REACTOME)
Signaling by Rho GTPases, organism-specific biosystemThe Rho family of small guanine nucleotide binding proteins is one of five generally recognized branches of the Ras superfamily. Like most Ras superfamily members, typical Rho proteins function as bi...
Signalling by NGF, organism-specific biosystem (from REACTOME)
Signalling by NGF, organism-specific biosystemNeurotrophins (NGF, BDNF, NT-3, NT-4/5) play pivotal roles in survival, differentiation, and plasticity of neurons in the peripheral and central nervous system. They are produced, and secreted in mi...
Vasopressin-regulated water reabsorption, organism-specific biosystem (from KEGG)
Vasopressin-regulated water reabsorption, organism-specific biosystemIn the kidney, the antidiuretic hormone vasopressin (AVP) is a critical regulator of water homeostasis by controlling the water movement from lumen to the interstitium for water reabsorption and adju...
Vasopressin-regulated water reabsorption, conserved biosystem (from KEGG)
Vasopressin-regulated water reabsorption, conserved biosystemIn the kidney, the antidiuretic hormone vasopressin (AVP) is a critical regulator of water homeostasis by controlling the water movement from lumen to the interstitium for water reabsorption and adju...
p75 NTR receptor-mediated signalling, organism-specific biosystem (from REACTOME)
p75 NTR receptor-mediated signalling, organism-specific biosystemBesides signalling through the tyrosine kinase receptors TRK A, B, and C, the mature neurotrophins NGF, BDNF, and NT3/4 signal through their common receptor p75NTR. NGF binding to p75NTR activates a ...
p75(NTR)-mediated signaling, organism-specific biosystem (from Pathway Interaction Database)
p75(NTR)-mediated signaling, organism-specific biosystem
p75(NTR)-mediated signaling
p75NTR regulates axonogenesis, organism-specific biosystem (from REACTOME)
p75NTR regulates axonogenesis, organism-specific biosystemp75NTR modulates axonal growth by regulating the activity of small GTPases like RHOA and RHOB, that control the state of actin polymerization. The best studied is RHOA. In its active, GTP-bound form,...

General gene information

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Homology

Homologs of the ARHGDIA gene: The ARHGDIA gene is conserved in Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, S.cerevisiae, K.lactis, E.gossypii, S.pombe, M.oryzae, N.crassa, A.thaliana, and rice.
Map Viewer (Mouse, Rat)
OrthoDB: The Hierarchical Catalog of Eukaryotic Orthologs

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
GTPase activator activity	IEA Inferred from Electronic Annotation more info
Rho GDP-dissociation inhibitor activity	IEA Inferred from Electronic Annotation more info
protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
Rho protein signal transduction	IEA Inferred from Electronic Annotation more info
cellular component movement	TAS Traceable Author Statement more info	PubMed
negative regulation of apoptotic process	TAS Traceable Author Statement more info	PubMed
negative regulation of axonogenesis	TAS Traceable Author Statement more info
negative regulation of cell adhesion	TAS Traceable Author Statement more info	PubMed
neurotrophin TRK receptor signaling pathway	TAS Traceable Author Statement more info
positive regulation of axonogenesis	TAS Traceable Author Statement more info
regulation of axonogenesis	TAS Traceable Author Statement more info
regulation of protein localization	IEA Inferred from Electronic Annotation more info
regulation of small GTPase mediated signal transduction	TAS Traceable Author Statement more info
small GTPase mediated signal transduction	TAS Traceable Author Statement more info

Component	Evidence Code	Pubs
cytoskeleton	TAS Traceable Author Statement more info	PubMed
cytosol	TAS Traceable Author Statement more info
immunological synapse	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: rho GDP-dissociation inhibitor 1

Names: rho GDP-dissociation inhibitor 1; rho GDI 1; rho-GDI alpha

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001185077.1 → NP_001172006.1 rho GDP-dissociation inhibitor 1 isoform a

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Variants 1 and 2 both encode isoform a.

Source sequence(s)

BC005851, BM552638

Consensus CDS

CCDS11788.1

UniProtKB/Swiss-Prot

P52565

Conserved Domains (1) summary

pfam02115
Location:1 – 201
Blast Score: 894

Rho_GDI; RHO protein GDP dissociation inhibitor
NM_001185078.1 → NP_001172007.1 rho GDP-dissociation inhibitor 1 isoform b

Status: VALIDATED

Description

Transcript Variant: This variant (3) lacks an alternate in-frame segment compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.

Source sequence(s)

AK300816, BC005851, DC406556

Consensus CDS

CCDS58609.1

UniProtKB/TrEMBL

A8MXW0

UniProtKB/Swiss-Prot

P52565

Related

ENSP00000383556, OTTHUMP00000259328, ENST00000400721, OTTHUMT00000441680

Conserved Domains (1) summary

pfam02115
Location:1 – 157
Blast Score: 608

Rho_GDI; RHO protein GDP dissociation inhibitor
NM_004309.4 → NP_004300.1 rho GDP-dissociation inhibitor 1 isoform a

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode isoform a.

Source sequence(s)

BC005851, DC406556

Consensus CDS

CCDS11788.1

UniProtKB/Swiss-Prot

P52565

Related

ENSP00000269321, OTTHUMP00000259327, ENST00000269321, OTTHUMT00000441679

Conserved Domains (1) summary

pfam02115
Location:1 – 201
Blast Score: 894

Rho_GDI; RHO protein GDP dissociation inhibitor

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 104

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p10 Primary Assembly

Genomic

NC_000017.10 Reference GRCh37.p10 Primary Assembly

Range

79825597..79829282, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000149.1 Alternate HuRef

Range

75227385..75230633, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate CHM1_1.0

Genomic

NC_018928.1 Alternate CHM1_1.0

Range

80940492..80944177, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	ABBA01019648.1 (34341..36464)	None
genomic	ABBA01019649.1	None
genomic	AC137723.6 (39703..43344)	None
genomic	AC145207.2 (115618..119259)	None
genomic	AMYH01008721.1 (41024..44318)	None
genomic	AMYH01008722.1	None
genomic	CH471099.1	EAW89697.1
		EAW89698.1
genomic	X63863.1	CAA45344.1
mRNA	AF498926.1	AAM21074.1
mRNA	AK293147.1	BAG56694.1
mRNA	AK300816.1	BAG62471.1
mRNA	AK312347.1	BAG35268.1
mRNA	BC005851.2	AAH05851.1
mRNA	BC005875.2	AAH05875.1
mRNA	BC007630.1	None
mRNA	BC008701.3	AAH08701.1
mRNA	BC009759.2	AAH09759.1
mRNA	BC013846.1	None
mRNA	BC016031.1	AAH16031.1
mRNA	BC016185.1	AAH16185.1
mRNA	BC024258.1	AAH24258.1
mRNA	BC027730.2	AAH27730.1
mRNA	BC028333.1	None
mRNA	BC075827.1	AAH75827.1
mRNA	BC106044.1	AAI06045.1
mRNA	BM552638.1	None
mRNA	BT006884.1	AAP35530.1
mRNA	CR456777.1	CAG33058.1
mRNA	D13989.1	BAA03096.1
mRNA	DC406556.1	None
mRNA	M97579.1	AAA36566.1
mRNA	X69550.1	CAA49281.1
other-genetic	DQ891117.2	ABM82043.1
other-genetic	DQ894296.2	ABM85222.1