TNPO1 transportin 1 [Homo sapiens] - Gene

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Summary

Official Symbol: TNPO1provided by HGNC
Official Full Name: transportin 1provided by HGNC
Primary source: HGNC:6401
See related: Ensembl:ENSG00000083312; HPRD:04210; MIM:602901; Vega:OTTHUMG00000100967
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: MIP; TRN; IPO2; MIP1; KPNB2
Summary: This gene encodes the beta subunit of the karyopherin receptor complex which interacts with nuclear localization signals to target nuclear proteins to the nucleus. The karyopherin receptor complex is a heterodimer of an alpha subunit which recognizes the nuclear localization signal and a beta subunit which docks the complex at nucleoporins. Alternate splicing of this gene results in two transcript variants encoding different proteins. [provided by RefSeq, Jul 2008]

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Genomic context

Location :: 5q13.2

Sequence :: Chromosome: 5; NC_000005.9 (72112418..72210215)

See TNPO1 in Epigenomics, MapViewer

Chromosome 5 - NC_000005.9 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Transportin binding might delay methylation of PABPN1 until after nuclear import.
Title: Arginine methylation of the nuclear poly(a) binding protein weakens the interaction with its nuclear import receptor, transportin.
Data demonstrate that Importin beta2 is necessary for localization of retinitis pigmentosa 2 (RP2) to the primary cilium, and identify two distinct binding sites of RP2, which interact independently with Importin beta2.
Title: Localization of retinitis pigmentosa 2 to cilia is regulated by Importin beta2.
Nuclear import of fused in sarcoma (FUS) is dependent on Transportin, and interference with this transport pathway leads to cytoplasmic redistribution and recruitment of fused in sarcoma (FUS) into stress granules.
Title: ALS-associated fused in sarcoma (FUS) mutations disrupt Transportin-mediated nuclear import.
Results suggest that hnRNP H and F are nuclear shuttling proteins whose posttranslational modifications may alter interaction with transportin 1, nuclear localization, and hence function.
Title: A glycine-rich domain of hnRNP H/F promotes nucleocytoplasmic shuttling and nuclear import through an interaction with transportin 1.
The interplay of the two negative regulators, transportin and importin beta, along with the positive regulator RanGTP, allows precise choreography of multiple cell cycle assembly events.
Title: Transportin regulates major mitotic assembly events: from spindle to nuclear pore assembly.
Ran in complex with importin-beta has a higher affinity for GTP. This feature is responsible for the generation of Ran-GTP from Ran-GDP by importin-beta.
Title: Importin-beta is a GDP-to-GTP exchange factor of Ran: implications for the mechanism of nuclear import.
we identify transportin-1 as the import receptor for ADAR1.
Title: RNA-regulated interaction of transportin-1 and exportin-5 with the double-stranded RNA-binding domain regulates nucleocytoplasmic shuttling of ADAR1.
Data suggest that increased MIP-1alpha/beta production enhances multiple myeloma cell binding to stromal cells by VLA-4-VCAM-1 adhesion, forming a "vicious cycle" between MM cell adhesion to stromal cells and MIP-1 production via VLA-4-VCAM-1 interaction.
Title: Vicious cycle between myeloma cell binding to bone marrow stromal cells via VLA-4-VCAM-1 adhesion and macrophage inflammatory protein-1alpha and MIP-1beta production.
TRN1-binding to CCR2 promotes its nuclear translocation in a TRN1-dependent manner.
Title: Chemokine receptor CCR2 undergoes transportin1-dependent nuclear translocation.
the 3.0 A crystal structure of unliganded Kap beta2, which consists of a superhelix of 20 HEAT repeats
Title: Conformational heterogeneity of karyopherin beta2 is segmental.

Submit: New GeneRIF Correction See all (14)

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Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

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HIV-1 protein interactions

Protein	Gene	Interaction	Pubs
Rev	rev	importin beta can inhibit the interaction of HIV-1 Rev with HIV-1 RRE RNA	PubMed
	rev	importin beta specifically binds to the Rev nuclear localization signal (NLS, amino acids 35-50) and mediates the nuclear import of Rev	PubMed
Tat	tat	The binding of HIV-1 Tat with importin beta is inhibited by RanGTP	PubMed
	tat	Importin beta binds to the HIV-1 Tat protein nuclear localization signal (NLS; amino acids 49-57) and mediates the nuclear import of Tat through a novel import pathway	PubMed
Vpr	vpr	HIV-1 Vpr co-localizes with karyopherin beta and regulates karyopherin beta-mediated docking of the HIV-1 preintegration complex at the nuclear envelope, however multiple reports indicate the two proteins do not directly interact	PubMed
integrase	gag-pol	Some reports indicate a possible role for the interactions between karyopherin alpha and beta with HIV-1 integrase in the nuclear import of HIV-1 preintegration complexes (PIC), while other reports indicate integrase is not involved in PIC nuclear import	PubMed
	gag-pol	Karyopherin beta binds to complexes between HIV-1 integrase (integrase/GST fusion protein) and karyopherin alpha, but not to integrase alone	PubMed
	gag-pol	Karyopherin alpha and beta are reported to interact with HIV-1 integrase (IN) to facilitate nuclear import of IN, however a conflicting report indicates nuclear accumulation of IN does not involve karyopherin alpha, beta 1, or beta 2 mediated pathways	PubMed
matrix	gag	Karyopherin beta mediates the nuclear import of HIV-1 preintegration complexes through karyopherin alpha, which binds directly to nuclear localization signals in HIV-1 Matrix	PubMed

Go to the HIV-1, Human Protein Interaction Database

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Products	Interactant	Other Gene	Source	Pubs	Description
Q92973	Q15717	ELAVL1	HPRD	PubMed
Q92973	Q16778	HIST2H2BE	HPRD	PubMed
Q92973	Q16695	HIST3H3	HPRD	PubMed
Q92973	P09651	HNRNPA1	HPRD	PubMed
Q92973	Q14103	HNRNPD	HPRD	PubMed
Q92973	P49790	NUP153	HPRD	PubMed
Q92973	P35658	NUP214	HPRD	PubMed
Q92973	P52948	NUP98	HPRD	PubMed
Q92973	Q9UBU9	NXF1	HPRD	PubMed
Q92973	Q86U42	PABPN1	HPRD	PubMed
Q92973	P62826	RAN	HPRD	PubMed
Q92973	P49792	RANBP2	HPRD	PubMed
Q92973	Q53T03	RGPD5	HPRD	PubMed
Q92973	P62750	RPL23A	HPRD	PubMed
Q92973	P46777	RPL5	HPRD	PubMed
Q92973	P62081	RPS7	HPRD	PubMed
Q92973	P09132	SRP19	HPRD	PubMed
Q92973	Q92973	TNPO1	HPRD	PubMed
BioGRID:110040	BioGRID:107358	CD4	BioGRID	PubMed	Affinity Capture-MS
BioGRID:110040	BioGRID:115331	HDAC5	BioGRID	PubMed	Affinity Capture-MS
BioGRID:110040	BioGRID:110455	MME	BioGRID	PubMed	Affinity Capture-MS
BioGRID:110040	BioGRID:110982	NUP98	BioGRID	PubMed	Reconstituted Complex
BioGRID:110040	BioGRID:113777	PABPN1	BioGRID	PubMed	Reconstituted Complex
BioGRID:110040	BioGRID:111837	RAN	BioGRID	PubMed	Co-crystal Structure; Reconstituted Complex
BioGRID:110040	BioGRID:123954	RGPD5	BioGRID	PubMed	Reconstituted Complex; Two-hybrid
BioGRID:110040	BioGRID:112067	RPL23A	BioGRID	PubMed	Affinity Capture-Western
BioGRID:110040	BioGRID:112606	SRP19	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex
BioGRID:110040	BioGRID:112781	TCEA2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:110040	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS

Function	Evidence Code	Pubs
nuclear localization sequence binding	TAS Traceable Author Statement more info	PubMed
protein binding	IPI Inferred from Physical Interaction more info	PubMed
protein transporter activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
RNA metabolic process	TAS Traceable Author Statement more info
gene expression	TAS Traceable Author Statement more info
interspecies interaction between organisms	IEA Inferred from Electronic Annotation more info
mRNA metabolic process	TAS Traceable Author Statement more info
protein import into nucleus, translocation	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
cytoplasm	IEA Inferred from Electronic Annotation more info
cytosol	TAS Traceable Author Statement more info
nucleus	IEA Inferred from Electronic Annotation more info

General protein information

Preferred Names: transportin-1

Names: transportin-1; importin 2; importin beta 2; importin beta-2; karyopherin beta-2; M9 region interaction protein; karyopherin (importin) beta 2

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NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_002270.3 → NP_002261.3 transportin-1 isoform 1

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1).

Source sequence(s)

AC008972, AC035140, U70322, U72069

Consensus CDS

CCDS43329.1

UniProtKB/Swiss-Prot

Q92973

Related

ENSP00000336712, OTTHUMP00000126960, ENST00000337273, OTTHUMT00000218577

Conserved Domains (3) summary

cd00020
Location:135 – 252
Blast Score: 117

ARM; Armadillo/beta-catenin-like repeats. An approximately 40 amino acid long tandemly repeated sequence motif first identified in the Drosophila segment polarity gene armadillo; these repeats were also found in the mammalian armadillo homolog beta-catenin, ...

COG5215
Location:22 – 767
Blast Score: 285

KAP95; Karyopherin (importin) beta [Intracellular trafficking and secretion]

cl04271
Location:42 – 109
Blast Score: 141

IBN_N; Importin-beta N-terminal domain
NM_153188.2 → NP_694858.1 transportin-1 isoform 2

Status: REVIEWED

Description

Transcript Variant: This variant (2) contains an alternate segment in the 5' UTR and lacks a portion of the 5' coding region, compared to variant 1. These differences cause translation initiation at a downstream AUG and a protein (isoform 2) with a shorter N-terminus compared to isoform 1.

Source sequence(s)

AC008953, AC008972, U70322, U72069

Consensus CDS

CCDS4016.1

UniProtKB/Swiss-Prot

Q92973

Related

ENSP00000425118, OTTHUMP00000222024, ENST00000506351, OTTHUMT00000368911

Conserved Domains (3) summary

cd00020
Location:127 – 244
Blast Score: 117

ARM; Armadillo/beta-catenin-like repeats. An approximately 40 amino acid long tandemly repeated sequence motif first identified in the Drosophila segment polarity gene armadillo; these repeats were also found in the mammalian armadillo homolog beta-catenin, ...

COG5215
Location:14 – 759
Blast Score: 285

KAP95; Karyopherin (importin) beta [Intracellular trafficking and secretion]

cl04271
Location:34 – 101
Blast Score: 141

IBN_N; Importin-beta N-terminal domain

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000005.9 Reference GRCh37.p5 Primary Assembly

Range

72112418..72210215

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000137.1 Alternate HuRef

Range

67317777..67415712

Download

GenBank, FASTA, Sequence Viewer (Graphics)

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Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AC008953.7	None
genomic	AC008972.8 (82104..92211)	None
genomic	AC035140.7	None
genomic	CH471084.1	EAW95713.1
		EAW95714.1
		EAW95715.1
mRNA	AK055669.1	None
mRNA	AK124768.1	None
mRNA	AK299671.1	BAG61582.1
mRNA	AK301021.1	BAG62638.1
mRNA	AK308177.1	None
mRNA	BC040340.1	AAH40340.1
mRNA	U70322.1	AAC50723.1
mRNA	U72069.1	AAB58254.1
mRNA	U72395.1	AAB68948.1
other-genetic	EU176628.1	ABW03429.1