ARF6 ADP-ribosylation factor 6 [Homo sapiens] - Gene

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Summary

Official Symbol: ARF6provided by HGNC
Official Full Name: ADP-ribosylation factor 6provided by HGNC
Primary source: HGNC:659
See related: Ensembl:ENSG00000165527; HPRD:02714; MIM:600464; Vega:OTTHUMG00000140296
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: DKFZp564M0264
Summary: This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7. [provided by RefSeq, Jul 2008]

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Genomic context

Location :: 14q21.3

Sequence :: Chromosome: 14; NC_000014.8 (50359736..50363772)

See ARF6 in Epigenomics, MapViewer

Chromosome 14 - NC_000014.8 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

a novel class III PI3K-ARF6 axis pathway mediates TLR9 signaling by regulating the cellular uptake of CpG ODN.
Title: Pivotal role of ADP-ribosylation factor 6 in Toll-like receptor 9-mediated immune signaling.
CNK3 and IPCEF1 produce a single protein that is required for HGF dependent Arf6 activation and migration.
Title: CNK3 and IPCEF1 produce a single protein that is required for HGF dependent Arf6 activation and migration.
Data suggest that GEP100-Arf6-AMAP1-cortactin pathway, activated by VEGFR2, appears to be common in angiogenesis and cancer invasion and metastasis, and provides their new therapeutic targets.
Title: GEP100-Arf6-AMAP1-cortactin pathway frequently used in cancer invasion is activated by VEGFR2 to promote angiogenesis.
targeting of FIP3-positive endosomal vesicles to the Flemming body in the abscission phase depends on Arf6.
Title: Distinct roles of Rab11 and Arf6 in the regulation of Rab11-FIP3/arfophilin-1 localization in mitotic cells.
findings unravel a novel mechanism of separation of ARF6 activation and effector function, ensuring that fast recycling may be determined at the level of receptor incorporation into clathrin-coated p
Title: Decoupling of activation and effector binding underlies ARF6 priming of fast endocytic recycling.
ARF6 can affect ErbB-3 nuclear localization in prostate cancer cells.
Title: Macropinocytosis inhibitors and Arf6 regulate ErbB3 nuclear localization in prostate cancer cells.
a novel phospholipid-regulated antiangiogenic signaling pathway whereby Sema3E activates Arf6 through Plexin-D1 and consequently controls integrin-mediated endothelial cell attachment to the extracellular matrix and migration.
Title: Phosphatidylinositol-4-phosphate 5-kinase and GEP100/Brag2 protein mediate antiangiogenic signaling by semaphorin 3E-plexin-D1 through Arf6 protein.
Perturbations of endosome morphology associated with Ras-induced non-apoptotic cell death are due to downstream activation of Rac1 combined with reciprocal inactivation of Arf6.
Title: Induction of nonapoptotic cell death by activated Ras requires inverse regulation of Rac1 and Arf6.
Efficient early HIV-1 infection of CD4(+) T lymphocytes requires Arf6-coordinated plasma membrane dynamics that promote viral fusion and entry.
Title: HIV-1 requires Arf6-mediated membrane dynamics to efficiently enter and infect T lymphocytes.
In the group of squamous cell lung cancer Arf6 expression elevation was observed more often.
Title: [Arf6, RalA and BIRC5 protein expression in non small cell lung cancer].

Submit: New GeneRIF Correction See all (75)

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Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

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HIV-1 protein interactions

Protein	Gene	Interaction	Pubs
Nef	nef	HIV-1 Nef-mediated downregulation of MHC-I has been reported to be regulated by ARF6, however a separate report indicates the apparent Arf6 dependency of Nef-mediated MHC class I down-modulation is due to nonspecific perturbations in membrane traffic	PubMed
	nef	The interaction of HIV-1 Nef and phosphatidylinositol-3-kinase (PI3K) activates the expression of ADP-ribosylation factor 6 (ARF6) in human melanoma cells	PubMed
Pr55(Gag)	gag	Overexpression of an active form of Arf6 (Arf6/Q67L), which induces the formation of PI(4,5)P2-enriched endosomal structures, severely reduces HIV-1 Gag virus production by redirecting Gag to PI(4,5)P2-enriched vesicles	PubMed

Go to the HIV-1, Human Protein Interaction Database

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Products	Interactant	Other Gene	Source	Pubs	Description
NP_001654.1			BIND	PubMed	ARP6 interacts with GTP. This interaction was modeled on a demonstrated interaction between ARF6 and GTP from unspecified species.
NP_001654.1			BIND	PubMed	ARP6 interacts with GDP. This interaction was modeled on a demonstrated interaction between ARF6 and GDP from unspecified species.
P62330	Q10567	AP1B1	HPRD	PubMed
P62330	O00203	AP3B1	HPRD	PubMed
P62330	O14617	AP3D1	HPRD	PubMed
P62330	P53365	ARFIP2	HPRD	PubMed
P62330	P49407	ARRB1	HPRD	PubMed
P62330	P32121	ARRB2	HPRD	PubMed
P62330	O43150	ASAP2	HPRD	PubMed
P62330	Q8TDY4	ASAP3	HPRD	PubMed
P62330	P27449	ATP6V0C	HPRD	PubMed
P62330	P07384	CAPN1	HPRD	PubMed
P62330	P20309	CHRM3	HPRD	PubMed
P62330	Q99418	CYTH2	HPRD	PubMed
P62330	O00471	EXOC5	HPRD	PubMed
P62330	P15311	EZR	HPRD	PubMed
P62330	Q8N3R9	MPP5	HPRD	PubMed
P62330	Q99755	PIP5K1A	HPRD	PubMed
P62330	O60331	PIP5K1C	HPRD	PubMed
P62330	Q13393	PLD1	HPRD	PubMed
P62330	O75154	RAB11FIP3	HPRD	PubMed
P62330	Q8N972	ZNF709	HPRD	PubMed
BioGRID:106877	BioGRID:114116	AP3B1	BioGRID	PubMed	Reconstituted Complex
BioGRID:106877	BioGRID:115533	AP3S2	BioGRID	PubMed	Reconstituted Complex
BioGRID:106877	BioGRID:117174	ARFIP2	BioGRID	PubMed	Reconstituted Complex; Two-hybrid
BioGRID:106877	BioGRID:106901	ARRB1	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex
BioGRID:106877	BioGRID:106902	ARRB2	BioGRID	PubMed	Affinity Capture-Western
BioGRID:106877	BioGRID:119126	ASAP1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:106877	BioGRID:107553	CHRM3	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex
BioGRID:106877	BioGRID:114687	CYTH2	BioGRID	PubMed	Affinity Capture-Western; Biochemical Activity
BioGRID:106877	BioGRID:108070	DHPS	BioGRID	PubMed	Affinity Capture-MS
BioGRID:106877	BioGRID:108117	DYNC1H1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:106877	BioGRID:108276	EGFR	BioGRID	PubMed	PCA
BioGRID:106877	BioGRID:109898	EIF6	BioGRID	PubMed	Affinity Capture-MS
BioGRID:106877	BioGRID:123666	EPPK1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:106877	BioGRID:115884	EXOC5	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex; Two-hybrid
BioGRID:106877	BioGRID:117653	FBXO8	BioGRID	PubMed	Biochemical Activity; Co-localization
BioGRID:106877	BioGRID:108888	GART	BioGRID	PubMed	Affinity Capture-MS
BioGRID:106877	BioGRID:116775	GGA3	BioGRID	PubMed	Reconstituted Complex
BioGRID:106877	BioGRID:114353	IQGAP1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:106877	BioGRID:116966	KIAA0368	BioGRID	PubMed	Affinity Capture-MS
BioGRID:106877	BioGRID:115432	LRPPRC	BioGRID	PubMed	Affinity Capture-MS
BioGRID:106877	BioGRID:121122	MEPCE	BioGRID	PubMed	Affinity Capture-MS
BioGRID:106877	BioGRID:114296	PABPC4	BioGRID	PubMed	Affinity Capture-MS
BioGRID:106877	BioGRID:116969	PIP5K1C	BioGRID	PubMed	Affinity Capture-Western
BioGRID:106877	BioGRID:111353	PLD1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:106877	BioGRID:111834	RALA	BioGRID	PubMed	Affinity Capture-Western
BioGRID:106877	BioGRID:116067	RUVBL2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:106877	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western; Reconstituted Complex
BioGRID:106877	BioGRID:114134	YARS	BioGRID	PubMed	Affinity Capture-MS

Function	Evidence Code	Pubs
GTP binding	TAS Traceable Author Statement more info	PubMed
GTPase activity	TAS Traceable Author Statement more info	PubMed
nucleotide binding	IEA Inferred from Electronic Annotation more info
protein binding	IPI Inferred from Physical Interaction more info	PubMed
thioesterase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
GTP catabolic process	TAS Traceable Author Statement more info	PubMed
apoptotic process	IEA Inferred from Electronic Annotation more info
cell adhesion	TAS Traceable Author Statement more info	PubMed
cell differentiation	IEA Inferred from Electronic Annotation more info
cellular component movement	TAS Traceable Author Statement more info	PubMed
cortical actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
liver development	IEA Inferred from Electronic Annotation more info
negative regulation of receptor-mediated endocytosis	TAS Traceable Author Statement more info	PubMed
nervous system development	IEA Inferred from Electronic Annotation more info
positive regulation of actin filament polymerization	IMP Inferred from Mutant Phenotype more info	PubMed
positive regulation of establishment of protein localization in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
protein localization at cell surface	ISS Inferred from Sequence or Structural Similarity more info
protein transport	IEA Inferred from Electronic Annotation more info
regulation of Rac protein signal transduction	IDA Inferred from Direct Assay more info	PubMed
regulation of dendritic spine development	ISS Inferred from Sequence or Structural Similarity more info
regulation of filopodium assembly	IDA Inferred from Direct Assay more info	PubMed
ruffle organization	IDA Inferred from Direct Assay more info	PubMed
small GTPase mediated signal transduction	IEA Inferred from Electronic Annotation more info
vesicle-mediated transport	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
Golgi apparatus	IEA Inferred from Electronic Annotation more info
cell cortex	IDA Inferred from Direct Assay more info	PubMed
cytoplasm	IEA Inferred from Electronic Annotation more info
early endosome	IEA Inferred from Electronic Annotation more info
endosome	IDA Inferred from Direct Assay more info
endosome	TAS Traceable Author Statement more info	PubMed
endosome membrane	IEA Inferred from Electronic Annotation more info
filopodium membrane	IDA Inferred from Direct Assay more info	PubMed
intracellular	IEA Inferred from Electronic Annotation more info
membrane fraction	TAS Traceable Author Statement more info	PubMed
plasma membrane	IDA Inferred from Direct Assay more info	PubMed
recycling endosome	IEA Inferred from Electronic Annotation more info
ruffle	IDA Inferred from Direct Assay more info	PubMed

General protein information

Preferred Names: ADP-ribosylation factor 6

Names: ADP-ribosylation factor 6

NP_001654.1

EC 3.6.5.1

EC 3.6.5.2

EC 3.6.5.3

EC 3.6.5.4

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NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001663.3 → NP_001654.1 ADP-ribosylation factor 6

Status: REVIEWED

Source sequence(s)

AL121163, BC008918, BC030291, BM838197, DA217686

Consensus CDS

CCDS9695.1

UniProtKB/Swiss-Prot

P62330

UniProtKB/TrEMBL

Q6FGZ2

Related

ENSP00000298316, OTTHUMP00000178980, ENST00000298316, OTTHUMT00000276883

Conserved Domains (1) summary

cd04149
Location:5 – 172
Blast Score: 928

Arf6; ADP ribosylation factor 6 (Arf6)

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000014.8 Reference GRCh37.p5 Primary Assembly

Range

50359736..50363772

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000146.1 Alternate HuRef

Range

30484292..30488326

Download

GenBank, FASTA, Sequence Viewer (Graphics)

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Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AL627171.1 (150425..152320)	None
genomic	AY296206.1	AAP50257.1
genomic	CH471078.2	EAW65740.1
mRNA	AF047432.1	AAC39877.1
mRNA	AF493885.1	AAM12599.1
mRNA	AK313790.1	BAG36527.1
mRNA	AL117621.1	None
mRNA	AL121163.1	None
mRNA	BC002952.1	None
mRNA	BC008918.2	AAH08918.1
mRNA	BC030291.1	None
mRNA	BM838197.1	None
mRNA	BT019868.1	AAV38671.1
mRNA	CR541939.1	CAG46737.1
mRNA	CR541964.1	CAG46762.1
mRNA	DA217686.1	None
mRNA	M57763.1	AAA90928.1

Protein Accession	Links
Protein Accession	GenPept Link	UniProtKB Link
P62330.2	GenPept	UniProtKB/Swiss-Prot:P62330
Q5U025	GenPept	UniProtKB/TrEMBL:Q5U025
Q6FGZ2	GenPept	UniProtKB/TrEMBL:Q6FGZ2
Q6FH17	GenPept	UniProtKB/TrEMBL:Q6FH17