Rho1 [Drosophila melanogaster] - Gene

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Summary

Official Symbol: Rho1provided by Flybase
Primary source: FLYBASE:FBgn0014020
Locus tag: Dmel_CG8416
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: 8416; AAF01186; CG8416; D-Rho1; Dm Rho1; Dmel\CG8416; DmRHO-A; dnRho; DRho; dRho1; Drho1; DRho1; dRhoA; DrhoA; DRhoA; E3.10/J3.8; l(2)52Fa; l(2)k02107b; n(2)k07236; rho; Rho; RHO; Rho A; Rho GTPase; Rho kinase; rho1; RHO1; rhoA; RhoA; RhoN19

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Genomic context

Location :: 52E5-52E5

Sequence :: Chromosome: 2R; NT_033778.3 (11990191..11994734)

See Rho1 in Epigenomics, MapViewer

Chromosome 2R - NT_033778.3 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The RhoA fails to form a compact ring in late cytokinesis after Sti depletion, and this function requires Sti kinase activity.
Title: Sticky/Citron kinase maintains proper RhoA localization at the cleavage site during cytokinesis.
This study revealed a novel mechanism for controlling salivary gland lumen size, namely through Rho1-dependent actin polymerization and distribution and downregulation of apical phosphorylated moesin.
Title: Rho GTPase controls Drosophila salivary gland lumen size through regulation of the actin cytoskeleton and Moesin.
removal of a single copy of rho1 can suppress the pak phenotype
Title: Genetic evidence for antagonism between Pak protein kinase and Rho1 small GTPase signaling in regulation of the actin cytoskeleton during Drosophila oogenesis.
Results suggest that Crossveinless-c contributes to sprouting and subsequent growth of the anterior-posterior-oriented branches through negative regulation of Rho1.
Title: Crossveinless-c, the Drosophila homolog of tumor suppressor DLC1, regulates directional elongation of dendritic branches via down-regulating Rho1 activity.
Continuous autophagy was required for integrin-mediated hemocyte spreading and Rho1-induced cell protrusions.
Title: Conserved role for autophagy in Rho1-mediated cortical remodeling and blood cell recruitment.
disruption of Rho1 suppresses apical constriction and invagination in Adenomatous polyposis coli null cells
Title: Apical constriction and invagination downstream of the canonical Wnt signaling pathway require Rho1 and Myosin II.
Results suggest that Rho1 functions at the cell cortex to regulate JNK activity and implicate Rho1 and moesin in epithelial cell survival.
Title: Rho1 regulates apoptosis via activation of the JNK signaling pathway at the plasma membrane.
analysis of second-site noncomplementation screen for modifiers of Rho1 signaling during imaginal disc morphogenesis in Drosophila
Title: A second-site noncomplementation screen for modifiers of Rho1 signaling during imaginal disc morphogenesis in Drosophila.
in addition to its interaction with Mwh Rho1 has functions in wing planar polarity that are parallel to and upstream of fz.
Title: Rho1 has multiple functions in Drosophila wing planar polarity.
Results establish Wash and Rho as regulators of both linear- and branched-actin networks, and suggest an Arp2/3-mediated mechanism for how cells might coordinately regulate these structures.
Title: Wash functions downstream of Rho and links linear and branched actin nucleation factors.

Submit: New GeneRIF Correction See all (20)

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Interactions

Products	Interactant	Other Gene	Source	Pubs	Description
BioGRID:62500	BioGRID:59367	CG1753	BioGRID	PubMed	Two-hybrid
BioGRID:62500	BioGRID:67391	CG4367	BioGRID	PubMed	Two-hybrid
BioGRID:62500	BioGRID:62515	CG8446	BioGRID	PubMed	Two-hybrid
BioGRID:62500	BioGRID:63764	Cdc37	BioGRID	PubMed	Phenotypic Suppression
BioGRID:62500	BioGRID:71956	Mbs	BioGRID	PubMed	Phenotypic Enhancement; Phenotypic Suppression
BioGRID:62500	BioGRID:58274	Moe	BioGRID	PubMed	Phenotypic Suppression
BioGRID:62500	BioGRID:78234	Parp	BioGRID	PubMed	Phenotypic Suppression
BioGRID:62500	BioGRID:61787	Pkn	BioGRID	PubMed	Phenotypic Enhancement
BioGRID:62500	BioGRID:63689	Rac1	BioGRID	PubMed	Phenotypic Enhancement; Phenotypic Suppression
BioGRID:62500	BioGRID:59069	RhoGAPp190	BioGRID	PubMed	Phenotypic Enhancement
BioGRID:62500	BioGRID:65446	RhoGDI	BioGRID	PubMed	Affinity Capture-MS
BioGRID:62500	BioGRID:62615	RhoGEF2	BioGRID	PubMed	Phenotypic Suppression
BioGRID:62500	BioGRID:67480	Ubpy	BioGRID	PubMed	Two-hybrid
BioGRID:62500	BioGRID:65744	alpha-Cat	BioGRID	PubMed	Phenotypic Enhancement
BioGRID:62500	BioGRID:59814	capu	BioGRID	PubMed	Phenotypic Enhancement
BioGRID:62500	BioGRID:78256	cta	BioGRID	PubMed	Phenotypic Enhancement
BioGRID:62500	BioGRID:61303	dia	BioGRID	PubMed	Phenotypic Suppression
BioGRID:62500	BioGRID:58490	dsh	BioGRID	PubMed	Phenotypic Suppression
BioGRID:62500	BioGRID:69580	fz	BioGRID	PubMed	Phenotypic Suppression
BioGRID:62500	BioGRID:59717	lilli	BioGRID	PubMed	Phenotypic Suppression
BioGRID:62500	BioGRID:78268	p120ctn	BioGRID	PubMed	Phenotypic Enhancement
BioGRID:62500	BioGRID:64306	pbl	BioGRID	PubMed	Phenotypic Enhancement; Phenotypic Suppression
BioGRID:62500	BioGRID:68604	plexB	BioGRID	PubMed	Phenotypic Suppression
BioGRID:62500	BioGRID:71089	rin	BioGRID	PubMed	Phenotypic Enhancement
BioGRID:62500	BioGRID:63218	robo	BioGRID	PubMed	Phenotypic Enhancement
BioGRID:62500	BioGRID:68714	rok	BioGRID	PubMed	Two-hybrid

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General gene information

Homology

Homologs of the Rho1 gene: The Rho1 gene is conserved in human, chimpanzee, dog, cow, mouse, rat, chicken, mosquito, C.elegans, and .

Pathways from BioSystems

Axon guidance, organism-specific biosystem (from REACTOME)
Axon guidance, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Axonal growth inhibition (RHOA activation), organism-specific biosystem (from REACTOME)
Axonal growth inhibition (RHOA activation), organism-specific biosystemcomputationally inferred pathway (not manually curated)
Developmental Biology, organism-specific biosystem (from REACTOME)
Developmental Biology, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Endocytosis, organism-specific biosystem (from KEGG)
Endocytosis, organism-specific biosystemEndocytosis is a mechanism for cells to remove ligands, nutrients, and plasma membrane (PM) proteins, and lipids from the cell surface, bringing them into the cell interior. Transmembrane proteins en...
Endocytosis, conserved biosystem (from KEGG)
Endocytosis, conserved biosystemEndocytosis is a mechanism for cells to remove ligands, nutrients, and plasma membrane (PM) proteins, and lipids from the cell surface, bringing them into the cell interior. Transmembrane proteins en...
G alpha (12/13) signalling events, organism-specific biosystem (from REACTOME)
G alpha (12/13) signalling events, organism-specific biosystemcomputationally inferred pathway (not manually curated)
G beta:gamma signalling through PI3Kgamma, organism-specific biosystem (from REACTOME)
G beta:gamma signalling through PI3Kgamma, organism-specific biosystemcomputationally inferred pathway (not manually curated)
G-protein beta:gamma signalling, organism-specific biosystem (from REACTOME)
G-protein beta:gamma signalling, organism-specific biosystemcomputationally inferred pathway (not manually curated)
GPCR downstream signaling, organism-specific biosystem (from REACTOME)
GPCR downstream signaling, organism-specific biosystemcomputationally inferred pathway (not manually curated)
GPVI-mediated activation cascade, organism-specific biosystem (from REACTOME)
GPVI-mediated activation cascade, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Hemostasis, organism-specific biosystem (from REACTOME)
Hemostasis, organism-specific biosystemcomputationally inferred pathway (not manually curated)
NGF signalling via TRKA from the plasma membrane, organism-specific biosystem (from REACTOME)
NGF signalling via TRKA from the plasma membrane, organism-specific biosystemcomputationally inferred pathway (not manually curated)
PI3K/AKT activation, organism-specific biosystem (from REACTOME)
PI3K/AKT activation, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Platelet activation, signaling and aggregation, organism-specific biosystem (from REACTOME)
Platelet activation, signaling and aggregation, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Rho GTPase cycle, organism-specific biosystem (from REACTOME)
Rho GTPase cycle, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Sema4D in semaphorin signaling, organism-specific biosystem (from REACTOME)
Sema4D in semaphorin signaling, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Sema4D induced cell migration and growth-cone collapse, organism-specific biosystem (from REACTOME)
Sema4D induced cell migration and growth-cone collapse, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Semaphorin interactions, organism-specific biosystem (from REACTOME)
Semaphorin interactions, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Signal Transduction, organism-specific biosystem (from REACTOME)
Signal Transduction, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Signaling by GPCR, organism-specific biosystem (from REACTOME)
Signaling by GPCR, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Signaling by Rho GTPases, organism-specific biosystem (from REACTOME)
Signaling by Rho GTPases, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Signalling by NGF, organism-specific biosystem (from REACTOME)
Signalling by NGF, organism-specific biosystemcomputationally inferred pathway (not manually curated)
TGF-beta signaling pathway, organism-specific biosystem (from KEGG)
TGF-beta signaling pathway, organism-specific biosystemThe transforming growth factor-beta (TGF-beta) family members, which include TGF-betas, activins and bone morphogenetic proteins (BMPs), are structurally related secreted cytokines found in species r...
TGF-beta signaling pathway, conserved biosystem (from KEGG)
TGF-beta signaling pathway, conserved biosystemThe transforming growth factor-beta (TGF-beta) family members, which include TGF-betas, activins and bone morphogenetic proteins (BMPs), are structurally related secreted cytokines found in species r...
Wnt signaling pathway, organism-specific biosystem (from KEGG)
Wnt signaling pathway, organism-specific biosystemWnt proteins are secreted morphogens that are required for basic developmental processes, such as cell-fate specification, progenitor-cell proliferation and the control of asymmetric cell division, i...
Wnt signaling pathway, conserved biosystem (from KEGG)
Wnt signaling pathway, conserved biosystemWnt proteins are secreted morphogens that are required for basic developmental processes, such as cell-fate specification, progenitor-cell proliferation and the control of asymmetric cell division, i...
p75 NTR receptor-mediated signalling, organism-specific biosystem (from REACTOME)
p75 NTR receptor-mediated signalling, organism-specific biosystemcomputationally inferred pathway (not manually curated)
p75NTR regulates axonogenesis, organism-specific biosystem (from REACTOME)
p75NTR regulates axonogenesis, organism-specific biosystemcomputationally inferred pathway (not manually curated)

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
GTP binding	IEA Inferred from Electronic Annotation more info
GTPase activity	ISS Inferred from Sequence or Structural Similarity more info
GTPase activity	NAS Non-traceable Author Statement more info	PubMed
GTPase activity	TAS Traceable Author Statement more info	PubMed
kinase binding	IPI Inferred from Physical Interaction more info	PubMed
protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
JNK cascade	NAS Non-traceable Author Statement more info	PubMed
Wnt receptor signaling pathway	NAS Non-traceable Author Statement more info	PubMed
actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
actin cytoskeleton organization	TAS Traceable Author Statement more info	PubMed
actin filament bundle assembly	IMP Inferred from Mutant Phenotype more info	PubMed
actin filament organization	IMP Inferred from Mutant Phenotype more info	PubMed
axon guidance	IMP Inferred from Mutant Phenotype more info	PubMed
border follicle cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
branch fusion, open tracheal system	TAS Traceable Author Statement more info	PubMed
cell elongation involved in imaginal disc-derived wing morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
cellularization	IMP Inferred from Mutant Phenotype more info	PubMed
cellularization	TAS Traceable Author Statement more info	PubMed
compound eye morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
cytokinesis	IMP Inferred from Mutant Phenotype more info	PubMed
cytoskeleton organization	TAS Traceable Author Statement more info	PubMed
dendrite morphogenesis	TAS Traceable Author Statement more info	PubMed
dendrite self-avoidance	IGI Inferred from Genetic Interaction more info
determination of left/right symmetry	IMP Inferred from Mutant Phenotype more info	PubMed
dorsal closure	IMP Inferred from Mutant Phenotype more info	PubMed
dorsal closure	TAS Traceable Author Statement more info	PubMed
NOT dorsal closure, amnioserosa morphology change	IMP Inferred from Mutant Phenotype more info	PubMed
dorsal closure, leading edge cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
dorsal closure, spreading of leading edge cells	IMP Inferred from Mutant Phenotype more info	PubMed
endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
epidermal growth factor receptor signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
establishment of imaginal disc-derived wing hair orientation	TAS Traceable Author Statement more info	PubMed
establishment of planar polarity	NAS Non-traceable Author Statement more info	PubMed
establishment of planar polarity	TAS Traceable Author Statement more info	PubMed
establishment of protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
establishment of tissue polarity	NAS Non-traceable Author Statement more info	PubMed
gastrulation	NAS Non-traceable Author Statement more info	PubMed
gastrulation involving germ band extension	IMP Inferred from Mutant Phenotype more info	PubMed
germ cell migration	TAS Traceable Author Statement more info	PubMed
germ-band extension	IMP Inferred from Mutant Phenotype more info	PubMed
glial cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
hemocyte migration	IMP Inferred from Mutant Phenotype more info	PubMed
imaginal disc-derived wing hair organization	IGI Inferred from Genetic Interaction more info	PubMed
imaginal disc-derived wing hair organization	IMP Inferred from Mutant Phenotype more info	PubMed
lumen formation, open tracheal system	IMP Inferred from Mutant Phenotype more info	PubMed
lumen formation, open tracheal system	TAS Traceable Author Statement more info	PubMed
maintenance of epithelial cell apical/basal polarity	IMP Inferred from Mutant Phenotype more info	PubMed
melanization defense response	IMP Inferred from Mutant Phenotype more info	PubMed
motor axon guidance	NAS Non-traceable Author Statement more info	PubMed
NOT muscle attachment	IMP Inferred from Mutant Phenotype more info	PubMed
NOT myofibril assembly	IMP Inferred from Mutant Phenotype more info	PubMed
neuroblast proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
ommatidial rotation	NAS Non-traceable Author Statement more info	PubMed
ommatidial rotation	TAS Traceable Author Statement more info	PubMed
open tracheal system development	IMP Inferred from Mutant Phenotype more info	PubMed
peripheral nervous system development	IMP Inferred from Mutant Phenotype more info	PubMed
posterior midgut invagination	IMP Inferred from Mutant Phenotype more info	PubMed
pseudocleavage involved in syncytial blastoderm formation	IMP Inferred from Mutant Phenotype more info	PubMed
regulation of Malpighian tubule size	IMP Inferred from Mutant Phenotype more info	PubMed
regulation of axonogenesis	IGI Inferred from Genetic Interaction more info	PubMed
regulation of cytoskeleton organization	IDA Inferred from Direct Assay more info	PubMed
regulation of embryonic cell shape	IMP Inferred from Mutant Phenotype more info	PubMed
regulation of tube length, open tracheal system	IMP Inferred from Mutant Phenotype more info	PubMed
response to DNA damage stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
salivary gland morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
small GTPase mediated signal transduction	IEA Inferred from Electronic Annotation more info
spiracle morphogenesis, open tracheal system	IMP Inferred from Mutant Phenotype more info	PubMed
ventral furrow formation	IMP Inferred from Mutant Phenotype more info	PubMed
wound healing	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
cell cortex	IDA Inferred from Direct Assay more info	PubMed
cell hair	IDA Inferred from Direct Assay more info	PubMed
cytoplasm	IDA Inferred from Direct Assay more info	PubMed

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General protein information

Preferred Names: CG8416 gene product from transcript CG8416-RB

Names: CG8416-PA; CG8416-PB; CG8416-PC; CG8416-PD; CG8416-PE; CG8416-PF; CG8416-PG; Rho GTPase; Rho1; Rho1-PA; Rho1-PB; Rho1-PC; Rho1-PD; Rho1-PE; Rho1-PF; Rho1-PG; RhoA; rho kinase; rho1; rho1 kinase

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NCBI Reference Sequences (RefSeq)

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033778.3

Range

11990191..11994734

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_134308.2 → NP_599135.1 Rho1, isoform B [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

A4UZI6

UniProtKB/Swiss-Prot

P48148

Related

FBpp0086354, FBtr0087212

Conserved Domains (1) summary

cd01870
Location:5 – 179
Blast Score: 1003

RhoA_like; Ras homology family A (RhoA)-like includes RhoA, RhoB and RhoC
NM_206128.1 → NP_995850.1 Rho1, isoform F [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

A4UZI6

UniProtKB/Swiss-Prot

P48148

Related

FBpp0089129, FBtr0087216

Conserved Domains (1) summary

cd01870
Location:5 – 179
Blast Score: 1003

RhoA_like; Ras homology family A (RhoA)-like includes RhoA, RhoB and RhoC
NM_057750.3 → NP_477098.1 Rho1, isoform A [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

A4UZI6

UniProtKB/Swiss-Prot

P48148

Related

FBpp0086353, FBtr0087211

Conserved Domains (1) summary

cd01870
Location:5 – 179
Blast Score: 1003

RhoA_like; Ras homology family A (RhoA)-like includes RhoA, RhoB and RhoC
NM_134309.1 → NP_599136.1 Rho1, isoform C [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

A4UZI6

UniProtKB/Swiss-Prot

P48148

Related

FBpp0086355, FBtr0087213

Conserved Domains (1) summary

cd01870
Location:5 – 179
Blast Score: 1003

RhoA_like; Ras homology family A (RhoA)-like includes RhoA, RhoB and RhoC
NM_206129.1 → NP_995851.1 Rho1, isoform E [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

A4UZI6

UniProtKB/Swiss-Prot

P48148

Related

FBpp0089128, FBtr0087215

Conserved Domains (1) summary

cd01870
Location:5 – 179
Blast Score: 1003

RhoA_like; Ras homology family A (RhoA)-like includes RhoA, RhoB and RhoC
NM_206127.1 → NP_995849.1 Rho1, isoform G [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

A4UZI6

UniProtKB/Swiss-Prot

P48148

Related

FBpp0089130, FBtr0087217

Conserved Domains (1) summary

cd01870
Location:5 – 179
Blast Score: 1003

RhoA_like; Ras homology family A (RhoA)-like includes RhoA, RhoB and RhoC
NM_166139.2 → NP_725524.1 Rho1, isoform D [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

A4UZI6

UniProtKB/Swiss-Prot

P48148

Related

FBpp0086356, FBtr0087214

Conserved Domains (1) summary

cd01870
Location:5 – 179
Blast Score: 1003

RhoA_like; Ras homology family A (RhoA)-like includes RhoA, RhoB and RhoC

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Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
mRNA	AF177872.1	AAF01184.1
genomic	AE013599.4	AAF58066.1
genomic	AF177871.1	AAF01183.1
mRNA	AF177873.1	AAF01185.1
genomic	GN079937.1	CAX86718.1
mRNA	L38311.1	AAA67042.1
genomic	AE013599.4	AAS64843.1
genomic	AE013599.4	AAS64842.1
mRNA	AY119536.1	AAM50190.1
genomic	CS026563.1	CAI61748.1
genomic	AE013599.4	AAS64844.1
genomic	AE013599.4	AAM70944.1
genomic	AE013599.4	AAM70945.1
genomic	AE013599.4	AAM70946.1
mRNA	BT010085.1	AAQ22554.1
mRNA	AF177874.1	AAF01186.1