INCENP inner centromere protein antigens 135/155kDa [Homo sapiens] - Gene

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Summary

Official Symbol: INCENPprovided by HGNC
Official Full Name: inner centromere protein antigens 135/155kDaprovided by HGNC
Primary source: HGNC:6058
See related: Ensembl:ENSG00000149503; HPRD:05103; MIM:604411; Vega:OTTHUMG00000167470
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: FLJ31633; MGC111393
Summary: In mammalian cells, 2 broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger,' or transiently interacting, proteins (reviewed by Choo, 1997). The constitutive proteins include CENPA (centromere protein A; MIM 117139), CENPB (MIM 117140), CENPC1 (MIM 117141), and CENPD (MIM 117142). The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle (Earnshaw and Mackay, 1994 [PubMed 8088460]). These include CENPE (MIM 117143); MCAK (MIM 604538); KID (MIM 603213); cytoplasmic dynein (e.g., MIM 600112); CliPs (e.g., MIM 179838); and CENPF/mitosin (MIM 600236). The inner centromere proteins (INCENPs) (Earnshaw and Cooke, 1991 [PubMed 1860899]), the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis (Cutts et al., 1999 [PubMed 10369859]).[supplied by OMIM, Mar 2008]

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Genomic context

Location :: 11q12.3

Sequence :: Chromosome: 11; NC_000011.9 (61891445..61920635)

See INCENP in Epigenomics, MapViewer

Chromosome 11 - NC_000011.9 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HP1alpha binding by INCENP or Shugoshin 1 (Sgo1) is dispensable for centromeric cohesion protection during mitosis of human cells, but might regulate yet unknown interphase functions of the chromosome passenger complex (CPC) or Sgo1 at the centromeres.
Title: Mitotic centromeric targeting of HP1 and its binding to Sgo1 are dispensable for sister-chromatid cohesion in human cells.
Results indicate that INCENP-Aurora B localized at centromeres/inner kinetochores is sufficient to mediate SAC activity upon spindle disruption.
Title: Centromere localization of INCENP-Aurora B is sufficient to support spindle checkpoint function.
Dephosphorylation of INCENP at anaphase and the concomitant relocation of the chromosomal passenger protein complex prevents kinetochore recruitment of mitotic checkpoint proteins.
Title: Relocation of the chromosomal passenger complex prevents mitotic checkpoint engagement at anaphase.
Data show that binding to INCENP is alone critical to the distinct function of Aurora B, and although G198 of Aurora A is required for TPX2 binding, N142G Aurora B retains INCENP binding and Aurora B function.
Title: Molecular distinctions between Aurora A and B: a single residue change transforms Aurora A into correctly localized and functional Aurora B.
High INCENP expression is associated with high grade non-Hodgkin B-cell lymphomas.
Title: INCENP (inner centromere protein) is overexpressed in high grade non-Hodgkin B-cell lymphomas.
Borealin and INCENP associate with the helical domain of Survivin to form a tight three-helical bundle.
Title: Structure of a Survivin-Borealin-INCENP core complex reveals how chromosomal passengers travel together.
protein truncation and in vitro mutagenesis,have identified the nucleolar localization sequences on INCENP
Title: Centromere RNA is a key component for the assembly of nucleoproteins at the nucleolus and centromere.
INCENP phosphorylation by Cdk1 is necessary for the recruitment of Plk1 to the kinetochore.
Title: Complex formation of Plk1 and INCENP required for metaphase-anaphase transition.
Data show that INCENP has an important role in stabilizing the chromosomal passenger complex, and that Borealin acts to promote binding of Survivin to INCENP.
Title: Survivin mediates targeting of the chromosomal passenger complex to the centromere and midbody.
association with inner centromere protein (INCENP) activates the novel chromosomal passenger protein, Aurora-C
Title: Direct association with inner centromere protein (INCENP) activates the novel chromosomal passenger protein, Aurora-C.

Submit: New GeneRIF Correction See all (14)

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Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

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Products	Interactant	Other Gene	Source	Pubs	Description
NP_064623.1	NP_001159.1	BIRC5	BIND	PubMed	Survivin interacts with INCENP. This interaction was modelled on a demonstrated interaction between human survivin and chicken INCENP.
NP_064623.1	NP_036249.1	CBX5	BIND	PubMed	HP1 interacts with INCENP. This interaction was modelled on a demonstrated interaction between human HP1 and chicken INCENP.
Q9NQS7	Q96GD4	AURKB	HPRD	PubMed
Q9NQS7	Q9UQB9	AURKC	HPRD	PubMed
Q9NQS7	O15392	BIRC5	HPRD	PubMed
Q9NQS7	P45973	CBX5	HPRD	PubMed
Q9NQS7	Q53HL2	CDCA8	HPRD	PubMed
Q9NQS7	P0C0S5	H2AFZ	HPRD	PubMed
BioGRID:109831	BioGRID:112666	AURKA	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex
BioGRID:109831	BioGRID:114646	AURKB	BioGRID	PubMed	Affinity Capture-Western; Co-localization; Reconstituted Complex
BioGRID:109831	BioGRID:203547	Aurkb	BioGRID	PubMed	Affinity Capture-MS
BioGRID:109831	BioGRID:106829	BIRC5	BioGRID	PubMed	Affinity Capture-Western; Co-localization; Reconstituted Complex
BioGRID:109831	BioGRID:120446	CDCA8	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex
BioGRID:109831	BioGRID:113584	EVI5	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex
BioGRID:109831	BioGRID:109269	H2AFZ	BioGRID	PubMed	Reconstituted Complex; Two-hybrid
BioGRID:109831	BioGRID:200760	Incenp	BioGRID	PubMed	Affinity Capture-MS
BioGRID:109831	BioGRID:127395	SGOL1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:109831	BioGRID:160326	TUBA1A	BioGRID	PubMed	Co-fractionation
BioGRID:109831	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS

Function	Evidence Code	Pubs
protein binding	IPI Inferred from Physical Interaction more info

Process	Evidence Code	Pubs
M phase of mitotic cell cycle	TAS Traceable Author Statement more info
chromosome segregation	IMP Inferred from Mutant Phenotype more info	PubMed
cytokinesis	IMP Inferred from Mutant Phenotype more info	PubMed
mitotic cell cycle	TAS Traceable Author Statement more info
mitotic prometaphase	TAS Traceable Author Statement more info

Component	Evidence Code	Pubs
central element	IEA Inferred from Electronic Annotation more info
centromeric heterochromatin	IDA Inferred from Direct Assay more info	PubMed
chromosome	IEA Inferred from Electronic Annotation more info
chromosome, centromeric region	IDA Inferred from Direct Assay more info	PubMed
condensed chromosome kinetochore	IEA Inferred from Electronic Annotation more info
cytoplasm	IEA Inferred from Electronic Annotation more info
cytoskeleton	IEA Inferred from Electronic Annotation more info
cytosol	TAS Traceable Author Statement more info
kinetochore	IDA Inferred from Direct Assay more info	PubMed
microtubule	IEA Inferred from Electronic Annotation more info
midbody	IEA Inferred from Electronic Annotation more info
nucleus	IEA Inferred from Electronic Annotation more info
protein complex	IDA Inferred from Direct Assay more info	PubMed
spindle	IDA Inferred from Direct Assay more info	PubMed

General protein information

Preferred Names: inner centromere protein

Names: inner centromere protein; chromosomal passenger protein; inner centromere protein INCENP; binds and activates aurora-B and -C in vivo and in vitro

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NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001040694.1 → NP_001035784.1 inner centromere protein isoform 1

Status: VALIDATED

Source sequence(s)

AP002793, AW839685, BC098576, BC111732, CV569756

Consensus CDS

CCDS44624.1

UniProtKB/Swiss-Prot

Q9NQS7

Related

ENSP00000378295, OTTHUMP00000236001, ENST00000394818, OTTHUMT00000394723

Conserved Domains (2) summary

pfam12178
Location:5 – 42
Blast Score: 184

INCENP_N; Chromosome passenger complex (CPC) protein INCENP N terminal

pfam03941
Location:825 – 883
Blast Score: 189

INCENP_ARK-bind; Inner centromere protein, ARK binding region
NM_020238.2 → NP_064623.2 inner centromere protein isoform 2

Status: VALIDATED

Source sequence(s)

AP002793, AW839685, BC032678, BC098576, BC111732, CV569756

Consensus CDS

CCDS31582.1

UniProtKB/Swiss-Prot

Q9NQS7

Related

ENSP00000278849, OTTHUMP00000236061, ENST00000278849, OTTHUMT00000394850

Conserved Domains (2) summary

pfam12178
Location:5 – 42
Blast Score: 184

INCENP_N; Chromosome passenger complex (CPC) protein INCENP N terminal

pfam03941
Location:821 – 879
Blast Score: 188

INCENP_ARK-bind; Inner centromere protein, ARK binding region

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000011.9 Reference GRCh37.p5 Primary Assembly

Range

61891445..61920635

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000143.1 Alternate HuRef

Range

58219712..58248907

Download

GenBank, FASTA, Sequence Viewer (Graphics)

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Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AP002793.4 (48610..73612)	None
genomic	CH471076.1	EAW74003.1
		EAW74004.1
		EAW74005.1
mRNA	AF282265.1	AAF87584.1
mRNA	AK056195.1	BAG51645.1
mRNA	AK307633.1	None
mRNA	AK310714.1	None
mRNA	AW839685.1	None
mRNA	AY714053.1	AAU04398.1
mRNA	BC032678.1	None
mRNA	BC098576.1	AAH98576.1
mRNA	BC111732.1	None
mRNA	CV569756.1	None