ILK integrin-linked kinase [Homo sapiens] - Gene

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Summary

Official Symbol: ILKprovided by HGNC
Official Full Name: integrin-linked kinaseprovided by HGNC
Primary source: HGNC:6040
See related: Ensembl:ENSG00000166333; HPRD:03842; MIM:602366; Vega:OTTHUMG00000133407
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: P59; ILK-2; DKFZp686F1765
Summary: Transduction of extracellular matrix signals through integrins influences intracellular and extracellular functions, and appears to require interaction of integrin cytoplasmic domains with cellular proteins. Integrin-linked kinase (ILK), interacts with the cytoplasmic domain of beta-1 integrin. This gene encodes a serine/threonine protein kinase with 4 ankyrin-like repeats, which associates with the cytoplasmic domain of beta integrins and acts as a proximal receptor kinase regulating integrin-mediated signal transduction. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

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Genomic context

Location :: 11p15.4

Sequence :: Chromosome: 11; NC_000011.9 (6624964..6632101)

See ILK in Epigenomics, MapViewer

Chromosome 11 - NC_000011.9 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ILK mainly expressed in scar tissue with formation time shorter than 6 months, and it may affect scar vascularization by regulating gene expressions of KDR and Flt-1.
Title: [Expression of integrin-linked kinase in human hypertrophic scar and its relationship with angiogenesis].
IGFBP requires ILK to induce cell motility. The IGFBP2/integrin/ILK/NF-kappaB network functions as a physiologically active signaling pathway in vivo by driving glioma progression
Title: Insulin-like growth factor-binding protein 2-driven glioma progression is prevented by blocking a clinically significant integrin, integrin-linked kinase, and NF-κB network.
Integrin-linked kinase regulates vasomotor function by preventing endothelial nitric oxide synthase uncoupling in atherosclerosis.
Title: Integrin-linked kinase regulates vasomotor function by preventing endothelial nitric oxide synthase uncoupling: role in atherosclerosis.
the clinical relevance of ILK in hepatocarcinogenesis
Title: Integrin-linked kinase overexpression and its oncogenic role in promoting tumorigenicity of hepatocellular carcinoma.
ILK protein was expressed in myeloma cell lines and kinase activity was elevated when cocultured with bone marrow stromal cells
Title: Targeting integrin-linked kinase increases apoptosis and decreases invasion of myeloma cell lines and inhibits IL-6 and VEGF secretion from BMSCs.
Knocking down ILK gene expression with siRNA inhibited the elevation of VEGF and ICAM-1 induced by high glucose treatment in retinal pigment epithelium cells.
Title: Inhibition of high glucose-induced VEGF and ICAM-1 expression in human retinal pigment epithelium cells by targeting ILK with small interference RNA.
Wild type integrin-linked kinase (ILK) reversed the poor mobility of CREG knock-down HUVECs.
Title: Cellular repressor of E1A-stimulated genes regulates vascular endothelial cell migration by the ILK/AKT/mTOR/VEGF(165) signaling pathway.
results suggest that ILK gene transfection is an effective approach to augment angiogenic properties of EPCs in vitro and providing basis for clinical cell-based gene therapy in patients with pre-eclampsia
Title: Gene modification with integrin-linked kinase improves function of endothelial progenitor cells in pre-eclampsia in vitro.
In osteoblasts exposed to dexamethasone, ILK expression was slightly increased, whereas there was substantial reduction in that of beta1 integrin.
Title: Effect of dexamethasone on human osteoblasts in culture: involvement of β1 integrin and integrin-linked kinase.
Our study suggests that ILK is overexpressed in invasive bladder cancer and plays an important role in the EMT of bladder cancer via the control of E-cadherin and MMP-9 expression.
Title: The importance of integrin-linked kinase in the regulation of bladder cancer invasion.

Submit: New GeneRIF Correction See all (123)

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Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

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Products	Interactant	Other Gene	Source	Pubs	Description
NP_004508.1	NP_002202.2	ITGB1	BIND	PubMed	Integrin-beta-1 interacts with ILK.
NP_004508.1	NP_000203.2	ITGB3	BIND	PubMed	Integrin-beta-3 interacts with ILK.
NP_004508.1	NP_004978.2	LIMS1	BIND	PubMed	PINCH interacts with ILK.
NP_004508.1		PARVB	BIND	PubMed	An unspecified isoform of affixin interacts with ILK.
NP_004508.1	NP_066932.1	TMSB4X	BIND	PubMed	ILK interacts with Thymosin Beta-4.
Q13418	P31749	AKT1	HPRD	PubMed
Q13418	Q14790	CASP8	HPRD	PubMed
Q13418	P55211	CASP9	HPRD	PubMed
Q13418	CAA79476.1	CAV1	BIND	PubMed	ILK1 interacts with caveolin-1.
Q13418	Q03135	CAV1	HPRD	PubMed
Q13418	P49841	GSK3B	HPRD	PubMed
Q13418	Q9H0C8	ILKAP	HPRD	PubMed
Q13418	P05556	ITGB1	HPRD	PubMed
Q13418	P05107	ITGB2	HPRD	PubMed
Q13418	P05106	ITGB3	HPRD	PubMed
Q13418	P48059	LIMS1	HPRD	PubMed
Q13418	LIM and senescent cell antigen-like domains 2	LIMS2	HPRD	PubMed
Q13418	Q9NVD7	PARVA	HPRD	PubMed
Q13418	Q9HBI1	PARVB	HPRD	PubMed
Q13418	Q9HBI0	PARVG	HPRD	PubMed
Q13418	O15530	PDPK1	HPRD	PubMed
Q13418	O14974	PPP1R12A	HPRD	PubMed
Q13418	P49023	PXN	HPRD	PubMed
Q13418	P62328	TMSB4X	HPRD	PubMed
BioGRID:109824	BioGRID:119378	ACP6	BioGRID	PubMed	Affinity Capture-MS
BioGRID:109824	BioGRID:106710	AKT1	BioGRID	PubMed	Affinity Capture-Western; Biochemical Activity
BioGRID:109824	BioGRID:107031	AUP1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:109824	BioGRID:107572	CKB	BioGRID	PubMed	Two-hybrid
BioGRID:109824	BioGRID:114693	COPB2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:109824	BioGRID:116496	COPG1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:109824	BioGRID:108020	DDX3X	BioGRID	PubMed	Affinity Capture-MS
BioGRID:109824	BioGRID:128022	DHX36	BioGRID	PubMed	Affinity Capture-MS
BioGRID:109824	BioGRID:108390	ERH	BioGRID	PubMed	Affinity Capture-MS
BioGRID:109824	BioGRID:108431	EWSR1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:109824	BioGRID:120511	FANCI	BioGRID	PubMed	Affinity Capture-MS
BioGRID:109824	BioGRID:119102	GEMIN4	BioGRID	PubMed	Affinity Capture-MS
BioGRID:109824	BioGRID:120433	HEATR1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:109824	BioGRID:120260	HEATR2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:109824	BioGRID:109547	HSPB1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:109824	BioGRID:115886	IGF2BP1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:109824	BioGRID:109824	ILK	BioGRID	PubMed	Biochemical Activity
BioGRID:109824	BioGRID:123343	ILKAP	BioGRID	PubMed	Affinity Capture-Western; Two-hybrid
BioGRID:109824	BioGRID:109884	ITGA5	BioGRID	PubMed	Affinity Capture-Western
BioGRID:109824	BioGRID:113288	ITGAV	BioGRID	PubMed	Affinity Capture-Western
BioGRID:109824	BioGRID:109894	ITGB1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:109824	BioGRID:109896	ITGB3	BioGRID	PubMed	Affinity Capture-Western
BioGRID:109824	BioGRID:110175	LIMS1	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex; Two-hybrid
BioGRID:109824	BioGRID:120808	LIMS2	BioGRID	PubMed	Affinity Capture-Western
BioGRID:109824	BioGRID:110311	MARS	BioGRID	PubMed	Affinity Capture-MS
BioGRID:109824	BioGRID:110431	MIPEP	BioGRID	PubMed	Two-hybrid
BioGRID:109824	BioGRID:122103	MMS19	BioGRID	PubMed	Affinity Capture-MS
BioGRID:109824	BioGRID:110726	MYO1D	BioGRID	PubMed	Affinity Capture-MS
BioGRID:109824	BioGRID:114020	NCK2	BioGRID	PubMed	Reconstituted Complex
BioGRID:109824	BioGRID:117586	NOC2L	BioGRID	PubMed	Affinity Capture-MS
BioGRID:109824	BioGRID:120112	OTUD4	BioGRID	PubMed	Affinity Capture-MS
BioGRID:109824	BioGRID:120860	PARVA	BioGRID	PubMed	Two-hybrid
BioGRID:109824	BioGRID:118912	PARVB	BioGRID	PubMed	Affinity Capture-MS
BioGRID:109824	BioGRID:111196	PDPK1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:109824	BioGRID:111618	PRPSAP1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:109824	BioGRID:116502	PUF60	BioGRID	PubMed	Affinity Capture-MS
BioGRID:109824	BioGRID:111787	PXN	BioGRID	PubMed	Reconstituted Complex
BioGRID:109824	BioGRID:117405	RNF19A	BioGRID	PubMed	Two-hybrid
BioGRID:109824	BioGRID:112057	RPL13	BioGRID	PubMed	Two-hybrid
BioGRID:109824	BioGRID:112164	RSU1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:109824	BioGRID:112188	S100A9	BioGRID	PubMed	Affinity Capture-MS
BioGRID:109824	BioGRID:111285	SERPINA4	BioGRID	PubMed	Two-hybrid
BioGRID:109824	BioGRID:112361	SHC1	BioGRID	PubMed	Two-hybrid
BioGRID:109824	BioGRID:116250	SNRNP35	BioGRID	PubMed	Two-hybrid
BioGRID:109824	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS
BioGRID:109824	BioGRID:109349	ZBTB48	BioGRID	PubMed	Two-hybrid
BioGRID:109824	BioGRID:113487	ZNF133	BioGRID	PubMed	Two-hybrid
BioGRID:109824	BioGRID:113539	ZNF205	BioGRID	PubMed	Two-hybrid
BioGRID:109824	BioGRID:127842	ZNF579	BioGRID	PubMed	Two-hybrid
BioGRID:109824	BioGRID:127574	ZNF746	BioGRID	PubMed	Two-hybrid

Function	Evidence Code	Pubs
ATP binding	NAS Non-traceable Author Statement more info
SH3 domain binding	IEA Inferred from Electronic Annotation more info
integrin binding	IEA Inferred from Electronic Annotation more info
nucleotide binding	IEA Inferred from Electronic Annotation more info
protein binding	IPI Inferred from Physical Interaction more info	PubMed
protein kinase binding	IPI Inferred from Physical Interaction more info	PubMed
protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
signal transducer activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
branching involved in ureteric bud morphogenesis	IEA Inferred from Electronic Annotation more info
cell aging	IEA Inferred from Electronic Annotation more info
cell cycle arrest	IEA Inferred from Electronic Annotation more info
cell junction assembly	TAS Traceable Author Statement more info
cell proliferation	NAS Non-traceable Author Statement more info	PubMed
cell-matrix adhesion	NAS Non-traceable Author Statement more info
establishment or maintenance of epithelial cell apical/basal polarity	IEA Inferred from Electronic Annotation more info
fibril organization	IEA Inferred from Electronic Annotation more info
integrin-mediated signaling pathway	IDA Inferred from Direct Assay more info	PubMed
integrin-mediated signaling pathway	NAS Non-traceable Author Statement more info
myelin assembly	IEA Inferred from Electronic Annotation more info
negative regulation of cardiac muscle cell apoptosis	IEA Inferred from Electronic Annotation more info
negative regulation of neuron apoptosis	IEA Inferred from Electronic Annotation more info
negative regulation of protein kinase activity	IEA Inferred from Electronic Annotation more info
negative regulation of smooth muscle cell migration	IEA Inferred from Electronic Annotation more info
negative regulation of smooth muscle cell proliferation	IEA Inferred from Electronic Annotation more info
neuron projection morphogenesis	IEA Inferred from Electronic Annotation more info
peptidyl-serine phosphorylation	IEA Inferred from Electronic Annotation more info
positive regulation of BMP signaling pathway	IEA Inferred from Electronic Annotation more info
positive regulation of MAP kinase activity	IEA Inferred from Electronic Annotation more info
positive regulation of MAPK cascade	IEA Inferred from Electronic Annotation more info
positive regulation of axon extension	IEA Inferred from Electronic Annotation more info
positive regulation of canonical Wnt receptor signaling pathway	IMP Inferred from Mutant Phenotype more info
positive regulation of cell migration	IEA Inferred from Electronic Annotation more info
positive regulation of cell proliferation	IEA Inferred from Electronic Annotation more info
positive regulation of cell-matrix adhesion	IEA Inferred from Electronic Annotation more info
positive regulation of dendrite morphogenesis	IEA Inferred from Electronic Annotation more info
positive regulation of myoblast differentiation	IEA Inferred from Electronic Annotation more info
positive regulation of osteoblast differentiation	IEA Inferred from Electronic Annotation more info
positive regulation of phosphorylation	IMP Inferred from Mutant Phenotype more info
positive regulation of protein kinase B signaling cascade	IEA Inferred from Electronic Annotation more info
positive regulation of transcription, DNA-dependent	IMP Inferred from Mutant Phenotype more info
protein heterooligomerization	IEA Inferred from Electronic Annotation more info
protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
regulation of actin cytoskeleton organization	IEA Inferred from Electronic Annotation more info
regulation of cell growth	IEA Inferred from Electronic Annotation more info
regulation of signal transduction	IEA Inferred from Electronic Annotation more info
substrate adhesion-dependent cell spreading	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
axon	IEA Inferred from Electronic Annotation more info
cell-cell junction	IEA Inferred from Electronic Annotation more info
costamere	IEA Inferred from Electronic Annotation more info
cytoplasm	NAS Non-traceable Author Statement more info
cytosol	TAS Traceable Author Statement more info
dendritic shaft	IEA Inferred from Electronic Annotation more info
focal adhesion	IEA Inferred from Electronic Annotation more info
neuronal cell body	IEA Inferred from Electronic Annotation more info
plasma membrane	IEA Inferred from Electronic Annotation more info
protein complex	IEA Inferred from Electronic Annotation more info
stress fiber	IEA Inferred from Electronic Annotation more info
terminal button	IEA Inferred from Electronic Annotation more info

General protein information

Preferred Names: integrin-linked protein kinase

Names: integrin-linked protein kinase; ILK-1; p59ILK; integrin-linked kinase-2; 59 kDa serine/threonine-protein kinase

NP_001014794.1

EC 2.7.11.1

NP_001014795.1

EC 2.7.11.1

NP_004508.1

EC 2.7.11.1

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NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_029702.1 RefSeqGene

Range

5001..12138

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001014794.1 → NP_001014794.1 integrin-linked protein kinase

Status: REVIEWED

Description

Transcript Variant: This variant (2) lacks a segment in the 5' UTR and encodes the same protein, as compared to variant 1.

Source sequence(s)

BC001554, CB113885, U40282

Consensus CDS

CCDS7768.1

UniProtKB/Swiss-Prot

Q13418

Related

ENSP00000403487, OTTHUMP00000229991, ENST00000420936, OTTHUMT00000384513

Conserved Domains (5) summary

cd00204
Location:28 – 140
Blast Score: 299

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam07714
Location:193 – 446
Blast Score: 378

Pkinase_Tyr; Protein tyrosine kinase

pfam12796
Location:38 – 130
Blast Score: 241

Ank_2; Ankyrin repeats (3 copies)

pfam13637
Location:4 – 54
Blast Score: 81

Ank_4; Ankyrin repeats (many copies)

cl09925
Location:205 – 447
Blast Score: 343

PKc_like; Protein Kinases, catalytic domain
NM_001014795.1 → NP_001014795.1 integrin-linked protein kinase

Status: REVIEWED

Description

Transcript Variant: This variant (3) lacks the 5' exon but has an alternate segment at the 5' end, as compared to variant 1. It encodes the same protein as variant 1.

Source sequence(s)

BC001554, CR749220, U40282

Consensus CDS

CCDS7768.1

UniProtKB/Swiss-Prot

Q13418

Related

ENSP00000379975, OTTHUMP00000229996, ENST00000396751, OTTHUMT00000384519

Conserved Domains (5) summary

cd00204
Location:28 – 140
Blast Score: 299

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam07714
Location:193 – 446
Blast Score: 378

Pkinase_Tyr; Protein tyrosine kinase

pfam12796
Location:38 – 130
Blast Score: 241

Ank_2; Ankyrin repeats (3 copies)

pfam13637
Location:4 – 54
Blast Score: 81

Ank_4; Ankyrin repeats (many copies)

cl09925
Location:205 – 447
Blast Score: 343

PKc_like; Protein Kinases, catalytic domain
NM_004517.2 → NP_004508.1 integrin-linked protein kinase

Status: REVIEWED

Description

Transcript Variant: This variant (1) differs from variants 2 and 3 in the 5' UTR. Variants 1-3 encode the same protein.

Source sequence(s)

BC001554, CB113885, U40282

Consensus CDS

CCDS7768.1

UniProtKB/Swiss-Prot

Q13418

Related

ENSP00000299421, OTTHUMP00000164591, ENST00000299421, OTTHUMT00000257264

Conserved Domains (5) summary

cd00204
Location:28 – 140
Blast Score: 299

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam07714
Location:193 – 446
Blast Score: 378

Pkinase_Tyr; Protein tyrosine kinase

pfam12796
Location:38 – 130
Blast Score: 241

Ank_2; Ankyrin repeats (3 copies)

pfam13637
Location:4 – 54
Blast Score: 81

Ank_4; Ankyrin repeats (many copies)

cl09925
Location:205 – 447
Blast Score: 343

PKc_like; Protein Kinases, catalytic domain

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000011.9 Reference GRCh37.p5 Primary Assembly

Range

6624964..6632101

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000143.1 Alternate HuRef

Range

6283901..6291033

Download

GenBank, FASTA, Sequence Viewer (Graphics)

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Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AC091564.12 (1229..8366)	None
genomic	AF244139.1	AAF74449.1
genomic	AJ404847.1	CAB99253.1
genomic	CH471064.2	EAW68688.1
		EAW68689.1
		EAW68690.1
mRNA	AB209416.1	BAD92653.1
mRNA	AK293474.1	BAH11516.1
mRNA	AK296628.1	BAH12404.1
mRNA	AK314843.1	None
mRNA	BC001554.1	AAH01554.1
mRNA	CB113885.1	None
mRNA	CR407673.1	CAG28601.1
mRNA	CR749220.1	CAH18077.1
mRNA	U40282.1	AAC16892.1
other-genetic	DQ891172.2	ABM82098.1
other-genetic	DQ894355.2	ABM85281.1