aub aubergine [Drosophila melanogaster] - Gene

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Summary

Official Symbol: aubprovided by Flybase
Official Full Name: aubergineprovided by Flybase
Primary source: FLYBASE:FBgn0000146
Locus tag: Dmel_CG6137
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: Aub; AUB; CG6137; Dmel\CG6137; ms(2)32D; sti; sting

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Genomic context

Location :: 32C1-32C1; 2-39 cM

Sequence :: Chromosome: 2L; NT_033779.4 (10997819..11001489, complement)

See aub in Epigenomics, MapViewer

Chromosome 2L - NT_033779.4 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TP5 represses the excision of other P elements in the germ line through a combination of maternal and zygotic effects. The maternal component of this repression is impaired by heterozygous mutations in the aubergine gene.
Title: Maternal impairment of transposon regulation in Drosophila melanogaster by mutations in the genes aubergine, piwi and Suppressor of variegation 205.
this study provides the first direct evidence that interaction surface of Tud for Aub is composed of multiple Tud domains and our data suggest that in addition to the segment containing domains 7-11, the other Tud domains are used for Aub interaction.
Title: Novel role of specific Tudor domains in Tudor-Aubergine protein complex assembly and distribution during Drosophila oogenesis.
results support a role for Aub, independent of its function in RNA silencing, as a component of a nos mRNA localization complex
Title: Aubergine is a component of a nanos mRNA localization complex.
Cocrystal structures of an extended Tudor domain (eTud) of Drosophila Tudor with methylated peptides of Aubergine, a Piwi family protein, reveal that sDMA is recognized by an asparagine-gated aromatic cage
Title: Structural basis for methylarginine-dependent recognition of Aubergine by Tudor.
Germ plasm assembly requires symmetrically dimethylated modification of Aub by dPRMT5, which, in turn, is required for binding to Tudor.
Title: Arginine methylation of Aubergine mediates Tudor binding and germ plasm localization.
Studies show that Drosophila melanogaster has three PIWI proteins: PIWI, AUBERGINE (AUB), and ARGONAUTE3 (AGO3) and it has been shown that Drosophila PIWI is mostly a nuclear protein [10] and is involved in the asymmetric division of germline stem cells.
Title: PIWI, piRNAs, and germline stem cells: what's the link?
An increased amount of Aub interferes with the correct functioning of the somatic yellow hairpin RNAi, whereas the Aub reduction does not
Title: Different aubergine alleles confirm the specificity of different RNAi pathways in Drosophila melanogaster.
The Drosophila homologue of protein methyltransferase 5 (dPRMT5, csul/dart5)methylates arginine in Drosophila Piwi, Ago3 and Aub proteins in vivo. Arginine methylation of Piwi proteins is required for Ago3 and Aub stability.
Title: Arginine methylation of Piwi proteins catalysed by dPRMT5 is required for Ago3 and Aub stability.
An increased amount of AUBERGINE interferes with the regular functioning of the somatic RNA interference pathway.
Title: aubergine gene overexpression in somatic tissues of aubergine(sting) mutants interferes with the RNAi pathway of a yellow hairpin dsRNA in Drosophila melanogaster.
the first biochemical insights into gene silencing mechanisms mediated by Aub and piRNAs in fly testes.
Title: Gene silencing mechanisms mediated by Aubergine piRNA complexes in Drosophila male gonad.

Submit: New GeneRIF Correction See all (17)

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Interactions

Products	Interactant	Other Gene	Source	Pubs	Description
BioGRID:60589	BioGRID:57628	CG11380	BioGRID	PubMed	Two-hybrid
BioGRID:60589	BioGRID:63269	CG3085	BioGRID	PubMed	Two-hybrid
BioGRID:60589	BioGRID:66235	osk	BioGRID	PubMed	Phenotypic Suppression
BioGRID:60589	BioGRID:77545	p53	BioGRID	PubMed	Affinity Capture-Western

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General gene information

Pathways from BioSystems

Dorso-ventral axis formation, organism-specific biosystem (from KEGG)
Dorso-ventral axis formation, organism-specific biosystem
Dorso-ventral axis formation
Dorso-ventral axis formation, conserved biosystem (from KEGG)
Dorso-ventral axis formation, conserved biosystem
Dorso-ventral axis formation

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
RNA binding	IDA Inferred from Direct Assay more info	PubMed
piRNA binding	IDA Inferred from Direct Assay more info

Process	Evidence Code	Pubs
RNA interference	IMP Inferred from Mutant Phenotype more info	PubMed
RNA interference	TAS Traceable Author Statement more info	PubMed
chromatin silencing	IMP Inferred from Mutant Phenotype more info	PubMed
cystoblast division	ISS Inferred from Sequence or Structural Similarity more info
dorsal appendage formation	IMP Inferred from Mutant Phenotype more info	PubMed
karyosome formation	TAS Traceable Author Statement more info	PubMed
maintenance of pole plasm mRNA location	TAS Traceable Author Statement more info	PubMed
mitotic chromosome condensation	IMP Inferred from Mutant Phenotype more info
negative regulation of transposition	IMP Inferred from Mutant Phenotype more info	PubMed
nuclear mRNA splicing, via spliceosome	IC Inferred by Curator more info	PubMed
oocyte maturation	IMP Inferred from Mutant Phenotype more info	PubMed
oogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
oogenesis	TAS Traceable Author Statement more info	PubMed
pole cell formation	IGI Inferred from Genetic Interaction more info	PubMed
pole plasm protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
positive regulation of nuclear-transcribed mRNA poly(A) tail shortening	IMP Inferred from Mutant Phenotype more info
positive regulation of oskar mRNA translation	NAS Non-traceable Author Statement more info	PubMed
positive regulation of oskar mRNA translation	TAS Traceable Author Statement more info	PubMed
regulation of oskar mRNA translation	TAS Traceable Author Statement more info	PubMed
regulation of pole plasm oskar mRNA localization	IMP Inferred from Mutant Phenotype more info	PubMed
regulation of translational initiation	ISS Inferred from Sequence or Structural Similarity more info	PubMed
segmentation	IGI Inferred from Genetic Interaction more info
stem cell division	ISS Inferred from Sequence or Structural Similarity more info
targeting of mRNA for destruction involved in RNA interference	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
P granule	IDA Inferred from Direct Assay more info	PubMed
catalytic step 2 spliceosome	IDA Inferred from Direct Assay more info	PubMed
cytoplasm	IDA Inferred from Direct Assay more info	PubMed
microtubule associated complex	IDA Inferred from Direct Assay more info	PubMed
precatalytic spliceosome	IDA Inferred from Direct Assay more info	PubMed

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General protein information

Preferred Names: CG6137 gene product from transcript CG6137-RA

Names: AUBERGINE; CG6137-PA; CG6137-PC; aub-PA; aub-PC; aubergine; stellate-interacting gene

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NCBI Reference Sequences (RefSeq)

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033779.4

Range

10997819..11001489, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_057386.4 → NP_476734.1 aubergine, isoform A [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

O76922

Related

FBpp0079754, FBtr0080165

Conserved Domains (2) summary

cd02845
Location:272 – 392
Blast Score: 368

PAZ_piwi_like; PAZ domain, Piwi_like subfamily. In multi-cellular organisms, the Piwi protein appears to be essential for the maintenance of germline stem cells. In the Drosophila male germline, Piwi was shown to be involved in the silencing of retrotransposons in the ...

cd04658
Location:400 – 849
Blast Score: 1299

Piwi_piwi-like_Euk; Piwi_piwi-like_Euk: PIWI domain, Piwi-like subfamily found in eukaryotes. This domain is found in Piwi and closely related proteins, where it is believed to perform a crucial role in germline cells, via RNA silencing. RNA silencing refers to a group of ...
NM_001103674.2 → NP_001097144.1 aubergine, isoform C [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/TrEMBL

A8DYZ0

Related

FBpp0111705, FBtr0112793

Conserved Domains (2) summary

cd02845
Location:201 – 321
Blast Score: 366

PAZ_piwi_like; PAZ domain, Piwi_like subfamily. In multi-cellular organisms, the Piwi protein appears to be essential for the maintenance of germline stem cells. In the Drosophila male germline, Piwi was shown to be involved in the silencing of retrotransposons in the ...

cd04658
Location:329 – 778
Blast Score: 1293

Piwi_piwi-like_Euk; Piwi_piwi-like_Euk: PIWI domain, Piwi-like subfamily found in eukaryotes. This domain is found in Piwi and closely related proteins, where it is believed to perform a crucial role in germline cells, via RNA silencing. RNA silencing refers to a group of ...