Mad Mothers against dpp [Drosophila melanogaster] - Gene

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Summary

Official Symbol: Madprovided by Flybase
Official Full Name: Mothers against dppprovided by Flybase
Primary source: FLYBASE:FBgn0011648
Locus tag: Dmel_CG12399
Gene type: protein coding
RNA name: mothers against dpp
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: 2/23; apg; c28; CG12399; dMad; Dmel\CG12399; E(zen)2; En(vvl); l(2)k00237; l(2)K00237; mad; MAD; Mat; p-Mad; P-mad; P-Mad; pMad; pMAD; PMad; pSmad; Smad; Smad1

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Genomic context

Location :: 23D3-23D3; 2-7.7 cM

Sequence :: Chromosome: 2L; NT_033779.4 (3146484..3159630, complement)

See Mad in Epigenomics, MapViewer

Chromosome 2L - NT_033779.4 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Wg signaling via Zw3 and mad restricts self-renewal of sensory organ precursor cells
Title: Wg signaling via Zw3 and mad restricts self-renewal of sensory organ precursor cells in Drosophila.
Mad has distinct signal transduction roles in the BMP and Wnt pathways depending on its phosphorylation state.
Title: Phosphorylation of Mad controls competition between wingless and BMP signaling.
Nup154 controls nuclear localization of Mad transcription factor, but does not interact directly with it.
Title: Drosophila nucleoporin Nup154 controls cell viability, proliferation and nuclear accumulation of Mad transcription factor.
Yorkie and Mad physically bind each other, and 410 bp minimal enhancer of bantam that responds to Yorkie:Mad in vivo and in cultured cells, was identified.
Title: Cooperative regulation of growth by Yorkie and Mad through bantam.
Heterodimers of SAX and TKV play an important role in extending the BMP activity gradient by facilitating DPP diffusion and assisting GBB signaling through functional complexes with type II receptors.
Title: The interaction between two TGF-beta type I receptors plays important roles in ligand binding, SMAD activation, and gradient formation.
importin-beta11 function interacts with the bone morphogenic protein pathway to regulate a pool of phosphorylated mothers against decapentaplegic that must be present at the presynapse for its proper development and function
Title: Importin-beta11 regulates synaptic phosphorylated mothers against decapentaplegic, and thereby influences synaptic development and function at the Drosophila neuromuscular junction.
Mad depletion or overexpression produced Wingless-like embryonic segmentation phenotypes
Title: Mad is required for wingless signaling in wing development and segment patterning in Drosophila.
phosphorylation of Mad by Nmo ensures normal accumulation and distribution of Mad and thereby fine tunes BMP signaling in motor neurons
Title: Nemo kinase interacts with Mad to coordinate synaptic growth at the Drosophila neuromuscular junction.
Bone morphogenetic protein pathway components can repress Wg target gene expression by influencing the binding of Arm and dTcf.
Title: Inhibition of Drosophila Wg signaling involves competition between Mad and Armadillo/beta-catenin for dTcf binding.
Results describe the crystal structure of the MH2 domain of Mad, which closely matches those of other Smad MH2 domains, is reported at 3.2 A resolution.
Title: Structure of Drosophila Mad MH2 domain.

Submit: New GeneRIF Correction See all (16)

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Interactions

Products	Interactant	Other Gene	Source	Pubs	Description
BioGRID:59745	BioGRID:69653	Aldh-III	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:58700	CG11164	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:58732	CG11581	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:65141	CG12436	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:63211	CG13510	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:63648	CG13900	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:66008	CG15177	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:72813	CG18745	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:65803	CG31534	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:72543	CG32428	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:77508	CG33109	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:66280	CG33936	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:63213	CG42565	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:68004	CG4673	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:67010	CG5903	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:64409	CG6576	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:59176	CG6891	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:63816	CG8993	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:62020	CG9027	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:66588	Cpn	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:60939	CycE	BioGRID	PubMed	Phenotypic Suppression
BioGRID:59745	BioGRID:68567	CycG	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:66148	DNApol-iota	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:67099	Dad	BioGRID	PubMed	Phenotypic Suppression
BioGRID:59745	BioGRID:63455	Ir60a	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:59479	MED15	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:68568	Med	BioGRID	PubMed	Reconstituted Complex
BioGRID:59745	BioGRID:63634	Rev1	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:67915	Smg6	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:59611	Su(dx)	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:60561	UbcD2	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:66028	bcd	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:59806	bowl	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:67943	dan	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:57669	dor	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:59659	dpp	BioGRID	PubMed	Phenotypic Enhancement; Phenotypic Suppression
BioGRID:59745	BioGRID:60071	eya	BioGRID	PubMed	Phenotypic Suppression
BioGRID:59745	BioGRID:61050	fws	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:62687	lack	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:64283	lqf	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:60366	numb	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:68607	pan	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:65883	rev7	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:61598	sax	BioGRID	PubMed	Phenotypic Suppression
BioGRID:59745	BioGRID:62183	sca	BioGRID	PubMed	Phenotypic Enhancement
BioGRID:59745	BioGRID:61853	sec24	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:65300	skl	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:58848	sog	BioGRID	PubMed	Phenotypic Enhancement
BioGRID:59745	BioGRID:63443	tamo	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:59924	tkv	BioGRID	PubMed	Phenotypic Suppression
BioGRID:59745	BioGRID:61589	tor	BioGRID	PubMed	Phenotypic Suppression
BioGRID:59745	BioGRID:71024	tws	BioGRID	PubMed	Two-hybrid
BioGRID:59745	BioGRID:63430	yki	BioGRID	PubMed	Affinity Capture-Western
BioGRID:59745	BioGRID:66026	zen	BioGRID	PubMed	Phenotypic Enhancement; Two-hybrid

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General gene information

Pathways from BioSystems

Signal Transduction, organism-specific biosystem (from REACTOME)
Signal Transduction, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Signaling by BMP, organism-specific biosystem (from REACTOME)
Signaling by BMP, organism-specific biosystemcomputationally inferred pathway (not manually curated)
TGF Beta Signaling Pathway, organism-specific biosystem (from WikiPathways)
TGF Beta Signaling Pathway, organism-specific biosystem
TGF Beta Signaling Pathway
TGF-beta signaling pathway, organism-specific biosystem (from KEGG)
TGF-beta signaling pathway, organism-specific biosystemThe transforming growth factor-beta (TGF-beta) family members, which include TGF-betas, activins and bone morphogenetic proteins (BMPs), are structurally related secreted cytokines found in species r...
TGF-beta signaling pathway, conserved biosystem (from KEGG)
TGF-beta signaling pathway, conserved biosystemThe transforming growth factor-beta (TGF-beta) family members, which include TGF-betas, activins and bone morphogenetic proteins (BMPs), are structurally related secreted cytokines found in species r...

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
DNA binding	TAS Traceable Author Statement more info
RNA polymerase II activating transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
RNA polymerase II distal enhancer sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
RNA polymerase II transcription coactivator activity	IDA Inferred from Direct Assay more info	PubMed
protein binding	IPI Inferred from Physical Interaction more info	PubMed
sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
sequence-specific distal enhancer binding RNA polymerase II transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity	NAS Non-traceable Author Statement more info

Process	Evidence Code	Pubs
compound eye morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
dorsal closure	NAS Non-traceable Author Statement more info	PubMed
germ-line stem cell division	IMP Inferred from Mutant Phenotype more info	PubMed
germ-line stem cell division	TAS Traceable Author Statement more info	PubMed
germ-line stem cell maintenance	IMP Inferred from Mutant Phenotype more info	PubMed
heart development	TAS Traceable Author Statement more info	PubMed
histoblast morphogenesis	IMP Inferred from Mutant Phenotype more info
imaginal disc-derived leg morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
imaginal disc-derived wing morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
imaginal disc-derived wing vein morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
negative regulation of S phase of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
negative regulation of gene expression	IDA Inferred from Direct Assay more info	PubMed
negative regulation of salivary gland boundary specification	TAS Traceable Author Statement more info	PubMed
open tracheal system development	TAS Traceable Author Statement more info	PubMed
ovarian follicle cell development	IMP Inferred from Mutant Phenotype more info	PubMed
ovarian follicle cell development	TAS Traceable Author Statement more info	PubMed
positive regulation of synaptic growth at neuromuscular junction	IMP Inferred from Mutant Phenotype more info	PubMed
positive regulation of transcription from RNA polymerase II promoter	IEP Inferred from Expression Pattern more info	PubMed
positive regulation of transcription from RNA polymerase II promoter	IMP Inferred from Mutant Phenotype more info	PubMed
regulation of cell differentiation	IMP Inferred from Mutant Phenotype more info
regulation of transcription from RNA polymerase II promoter	NAS Non-traceable Author Statement more info	PubMed
somatic stem cell maintenance	IMP Inferred from Mutant Phenotype more info	PubMed
transforming growth factor beta receptor signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
transforming growth factor beta receptor signaling pathway	NAS Non-traceable Author Statement more info
transforming growth factor beta receptor signaling pathway	TAS Traceable Author Statement more info	PubMed
trunk segmentation	IMP Inferred from Mutant Phenotype more info	PubMed
wing disc anterior/posterior pattern formation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
cytoplasm	IDA Inferred from Direct Assay more info	PubMed
cytoplasm	TAS Traceable Author Statement more info
nucleus	IDA Inferred from Direct Assay more info	PubMed
nucleus	TAS Traceable Author Statement more info
transcription factor complex	IEA Inferred from Electronic Annotation more info

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General protein information

Preferred Names: CG12399 gene product from transcript CG12399-RA

Names: CG12399-PA; Mad-PA; apang; mother against decapentaplegic; mothers against decapentaplegi; mothers against decapentaplegic; mothers against dpp; phospho-Smad; phosphoSmad; phosphorylated smad

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NCBI Reference Sequences (RefSeq)

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033779.4

Range

3146484..3159630, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_057669.2 → NP_477017.1 mothers against dpp [Drosophila melanogaster]

Status: REVIEWED

UniProtKB/Swiss-Prot

P42003

Related

FBpp0077302, FBtr0077616

Conserved Domains (2) summary

cd10490
Location:23 – 146
Blast Score: 721

MH1_SMAD_1_5_9; N-terminal Mad Homology 1 (MH1) domain in SMAD1, SMAD5 and SMAD9 (also known as SMAD8)

cd10497
Location:255 – 455
Blast Score: 1134

MH2_SMAD_1_5_9; C-terminal Mad Homology 2 (MH2) domain in SMAD1, SMAD5 and SMAD9