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    DNAJB1 DnaJ (Hsp40) homolog, subfamily B, member 1 [ Homo sapiens ]

    Gene ID: 3337, updated on 11-May-2012
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    Summary

    Official Symbol
    DNAJB1provided by HGNC
    Official Full Name
    DnaJ (Hsp40) homolog, subfamily B, member 1provided by HGNC
    Primary source
    HGNC:5270
    See related
    Ensembl:ENSG00000132002; HPRD:05198; MIM:604572
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    Hdj1; Sis1; HSPF1; Hsp40; RSPH16B
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    Genomic context

    Location :
    19p13.2
    Sequence :
    Chromosome: 19; NC_000019.9 (14625582..14629201, complement)
    See DNAJB1 in Epigenomics, MapViewer

    Chromosome 19 - NC_000019.9Genomic Context describing neighboring genes Neighboring gene prostaglandin E receptor 1 (subtype EP1), 42kDa Neighboring gene GIPC PDZ domain containing family, member 1 Neighboring gene small nuclear ribonucleoprotein polypeptide G pseudogene Neighboring gene microRNA 639 Neighboring gene trans-2,3-enoyl-CoA reductase Neighboring gene NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 7, 18kDa

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    Genomic regions, transcripts, and products

    Go to nucleotideGraphics FASTA GenBank

    Go to reference sequence details
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    Bibliography

    Related articles in PubMed

    1. Hsp40 regulates the amount of keratin proteins via ubiquitin-proteasome pathway in cultured human cells. Yamazaki S, et al. Int J Mol Med, 2012 Feb. PMID 22075554.
    2. The mammalian disaggregase machinery: Hsp110 synergizes with Hsp70 and Hsp40 to catalyze protein disaggregation and reactivation in a cell-free system. Shorter J. PLoS One, 2011. PMID 22022600.
    3. Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels. Lee KA, et al. J Biol Chem, 2011 Dec 2. PMID 21987572.
    4. Systematic and quantitative assessment of the ubiquitin-modified proteome. Kim W, et al. Mol Cell, 2011 Oct 21. PMID 21906983.
    5. A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles. Wagner SA, et al. Mol Cell Proteomics, 2011 Oct. PMID 21890473.

    See all (66) citations in PubMed

    See citations in PubMed for homologs of this gene provided by HomoloGene

    GeneRIFs: Gene References Into Functions What's a GeneRIF?

    1. Hsp40 regulates the amount of keratin proteins via ubiquitin-proteasome pathway.
      Title: Hsp40 regulates the amount of keratin proteins via ubiquitin-proteasome pathway in cultured human cells.
    2. Mammalian Hsp110 (Apg-2), Hsp70 (Hsc70 or Hsp70) and Hsp40 (Hdj1) were necessary and sufficient to slowly dissolve large disordered aggregates and recover natively folded protein
      Title: The mammalian disaggregase machinery: Hsp110 synergizes with Hsp70 and Hsp40 to catalyze protein disaggregation and reactivation in a cell-free system.
    3. Cellular localization studies showed that NP and Hsp40 co-localize primarily in the nucleus. During IAV infection in mammalian cells, expression of NP coincided with the dissociation of P58(IPK) from Hsp40 and decrease PKR phosphorylation
      Title: Influenza A virus nucleoprotein exploits Hsp40 to inhibit PKR activation.
    4. HSF1 and major HSP's are expressed strongly in classical Hodgkin lymphoma.
      Title: Expression of heat shock proteins in classical Hodgkin lymphoma: correlation with apoptotic pathways and prognostic significance.
    5. Chaperone activity of proteins Hsp70 and Hdj1 in human leukemia u-937 cells were increased after heat shock.
      Title: Kinetics of chaperone activity of proteins Hsp70 and Hdj1 in human leukemia u-937 cells after preconditioning with thermal shock or compound u-133.
    6. Differential expression of Hsp40 and Hsp70 reciprocally regulates viral gene expression and replication in HIV-1 infection.
      Title: Reciprocal regulation of human immunodeficiency virus-1 gene expression and replication by heat shock proteins 40 and 70.
    7. The antiviral activity of overexpressed DNAJC14 occurs in a time- and dose-dependent manner.
      Title: Identification and characterization of the host protein DNAJC14 as a broadly active flavivirus replication modulator.
    8. The crystal structure of full-length Hdj1 was diffracted to 3.90A resolution.
      Title: Purification, crystallization and preliminary X-ray crystallographic analysis of the human heat-shock protein 40 Hdj1 and its C-terminal peptide-binding domain.
    9. Determined are crystal structures of the C-terminal peptide-binding domain of human Hsp40 Hdj1 (CTD) and of its complex with the C-terminal octapeptide of human Hsp70, (634')GPTIEEVD(641').
      Title: Peptide-binding sites as revealed by the crystal structures of the human Hsp40 Hdj1 C-terminal domain in complex with the octapeptide from human Hsp70.
    10. Data show that HSP70, GRP78/BiP and HSP40/DnaJ each independently suppressed hA misfolding.
      Title: The chaperone proteins HSP70, HSP40/DnaJ and GRP78/BiP suppress misfolding and formation of β-sheet-containing aggregates by human amylin: a potential role for defective chaperone biology in Type 2 diabetes.
    Submit: New GeneRIF Correction See all (22)
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    Phenotypes

    Review eQTL and phenotype association data in this region using PheGenI

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    HIV-1 protein interactions

    Protein Gene Interaction Pubs
    Nef nef HIV-1 Nef interacts with Hsp40, leading to increased Hsp40 translocation to the nucleus of infected cells and facilitation of viral gene expression by complex formation of Hsp40 with the cdk9-associated transcription complex PubMed
    nef HIV-1 Subtype C Nef upregulates the expression of Hsp40 in HIV-1 infected CEM cells and enhances the increased translocation of Hsp40 into the nucleus PubMed
    nef HIV-1 subtype C Nef interacts with Hsp40 in immunoprecipitation and immunofluorescence staining experiments PubMed

    Go to the HIV-1, Human Protein Interaction Database

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    Interactions

    Products Interactant Other Gene Complex Source Pubs Description
    NP_006136.1 NP_006588.1 HSPA8    BIND  PubMed Hsp40 interacts with the carboxy-terminal region of Hsc70. 
    P25685 Q01484 ANK2    HPRD  PubMed  
    P25685 Q9HCU9 BRMS1    HPRD  PubMed  
    P25685 Q13217 DNAJC3    HPRD  PubMed  
    P25685 Q00597 FANCC    HPRD  PubMed  
    P25685 P16278 GLB1    HPRD  PubMed  
    P25685 Q00613 HSF1    HPRD  PubMed  
    P25685 Heat shock 70 KD protein 1A HSPA1A    HPRD  PubMed  
    P25685 P15559 NQO1    HPRD  PubMed  
    P25685 Q15185 PTGES3    HPRD  PubMed  
    P25685 P00441 SOD1    HPRD  PubMed  
    P25685 Q9UNE7 STUB1    HPRD  PubMed  
    BioGRID:109569 BioGRID:106710 AKT1    BioGRID  PubMed Two-hybrid 
    BioGRID:109569 BioGRID:116748 ATG2A    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:109569 BioGRID:107491 CENPE    BioGRID  PubMed Two-hybrid 
    BioGRID:109569 BioGRID:107506 CFTR    BioGRID  PubMed Affinity Capture-Western 
    BioGRID:109569 BioGRID:159906 DNAJC3    BioGRID  PubMed Reconstituted Complex; Two-hybrid 
    BioGRID:109569 BioGRID:971788 EBNA-LP    BioGRID  PubMed Affinity Capture-MS; Affinity Capture-Western 
    BioGRID:109569 BioGRID:108472 FANCA    BioGRID  PubMed Two-hybrid 
    BioGRID:109569 BioGRID:108473 FANCC    BioGRID  PubMed Affinity Capture-Western; Reconstituted Complex 
    BioGRID:109569 BioGRID:109119 GPR37    BioGRID  PubMed Affinity Capture-Western 
    BioGRID:109569 BioGRID:109530 HSF1    BioGRID  PubMed Reconstituted Complex 
    BioGRID:109569 BioGRID:109540 HSPA4    BioGRID  PubMed Affinity Capture-Western; Reconstituted Complex 
    BioGRID:109569 BioGRID:109544 HSPA8    BioGRID  PubMed Reconstituted Complex 
    BioGRID:109569 BioGRID:246629 Hspa8    BioGRID  PubMed Reconstituted Complex; Two-hybrid 
    BioGRID:109569 BioGRID:110952 NRAS    BioGRID  PubMed Two-hybrid 
    BioGRID:109569 BioGRID:120112 OTUD4    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:109569 BioGRID:111105 PARK2    BioGRID  PubMed Affinity Capture-Western 
    BioGRID:109569 BioGRID:119355 PLEKHO1    BioGRID  PubMed Two-hybrid 
    BioGRID:109569 BioGRID:117417 POT1    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:109569 BioGRID:111550 PRKAA2    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:109569 BioGRID:116152 SEC61B    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:109569 BioGRID:118079 SERP1    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:109569 BioGRID:115563 STUB1    BioGRID  PubMed Affinity Capture-Western; Reconstituted Complex; Two-hybrid 
    BioGRID:109569 BioGRID:115223 TELO2    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:109569 BioGRID:230183 Ttll12    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:109569 BioGRID:113164 UBC    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:109569 BioGRID:117550 WIPI2    BioGRID  PubMed Affinity Capture-MS 
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    General gene information

    Markers

    Genotypes

    Homology

    • Homologs of the DNAJB1 gene: The DNAJB1 gene is conserved in chimpanzee, dog, cow, mouse, rat, chicken, zebrafish, C.elegans, S.cerevisiae, K.lactis, , S.pombe, , N.crassa, A.thaliana, and rice.
    • Map Viewer (Mouse, Rat)

    Pathways from BioSystems

    • Influenza A, organism-specific biosystem (from KEGG)
      Influenza A, organism-specific biosystemInfluenza is a contagious respiratory disease caused by influenza virus infection. Influenza A virus is responsible for both annual seasonal epidemics and periodic worldwide pandemics. Novel strains ...
    • Influenza A, conserved biosystem (from KEGG)
      Influenza A, conserved biosystemInfluenza is a contagious respiratory disease caused by influenza virus infection. Influenza A virus is responsible for both annual seasonal epidemics and periodic worldwide pandemics. Novel strains ...
    • Protein processing in endoplasmic reticulum, organism-specific biosystem (from KEGG)
      Protein processing in endoplasmic reticulum, organism-specific biosystemThe endoplasmic reticulum (ER) is a subcellular organelle where proteins are folded with the help of lumenal chaperones. Newly synthesized peptides enter the ER via the sec61 pore and are glycosylate...
    • Protein processing in endoplasmic reticulum, conserved biosystem (from KEGG)
      Protein processing in endoplasmic reticulum, conserved biosystemThe endoplasmic reticulum (ER) is a subcellular organelle where proteins are folded with the help of lumenal chaperones. Newly synthesized peptides enter the ER via the sec61 pore and are glycosylate...

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    heat shock protein binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    unfolded protein binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    chaperone cofactor-dependent protein refolding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    chaperone mediated protein folding requiring cofactor IEA
    Inferred from Electronic Annotation
    more info
    		  
    protein folding IEA
    Inferred from Electronic Annotation
    more info
    		  
    response to stress IEA
    Inferred from Electronic Annotation
    more info
    		  
    response to unfolded protein TAS
    Traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    nucleolus IEA
    Inferred from Electronic Annotation
    more info
    		  
    nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    soluble fraction IEA
    Inferred from Electronic Annotation
    more info
    		  
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    General protein information

    Preferred Names
    dnaJ homolog subfamily B member 1
    Names
    dnaJ homolog subfamily B member 1
    hDj-1
    human DnaJ protein 1
    heat shock protein 40
    dnaJ protein homolog 1
    heat shock 40kD protein 1
    radial spoke 16 homolog B
    heat shock 40 kDa protein 1
    DnaJ (Hsp40) homolog, subfmaily B, member 1
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    NCBI Reference Sequences (RefSeq)

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_006145.1 → NP_006136.1  dnaJ homolog subfamily B member 1

      Status: PROVISIONAL

      Source sequence(s)
      D49547
      Consensus CDS
      CCDS12312.1
      UniProtKB/Swiss-Prot
      P25685
      UniProtKB/TrEMBL
      Q6FHS4
      Related
      ENSP00000254322, ENST00000254322
      Conserved Domains (3) summary
      COG0484
      Location:1 – 340
      Blast Score: 491
      DnaJ; DnaJ-class molecular chaperone with C-terminal Zn finger domain [Posttranslational modification, protein turnover, chaperones]
      cd06257
      Location:4 – 57
      Blast Score: 238
      DnaJ; DnaJ domain or J-domain. DnaJ/Hsp40 (heat shock protein 40) proteins are highly conserved and play crucial roles in protein translation, folding, unfolding, translocation, and degradation. They act primarily by stimulating the ATPase activity of Hsp70s, ...
      cd10747
      Location:162 – 326
      Blast Score: 411
      DnaJ_C; C-terminal substrate binding domain of DnaJ and HSP40

    RefSeqs of Annotated Genomes: Build 37.3

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh37.p5 Primary Assembly

    Genomic

    1. NC_000019.9 Reference GRCh37.p5 Primary Assembly

      Range
      14625582..14629201, complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate HuRef

    Genomic

    1. AC_000151.1 Alternate HuRef

      Range
      14195571..14199191, complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
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    Related Sequences

    Nucleotide Protein
    Heading Accession and Version
    genomic AC012318.7 (30395..34014) None
    genomic CH471106.1 EAW84432.1
      EAW84433.1
    genomic D85429.1 BAA12819.1
    genomic JA462410.1 CCD35253.1
    mRNA AK092989.1 None
    mRNA AK301817.1 BAG63264.1
    mRNA AK312624.1 BAG35510.1
    mRNA BC002352.2 AAH02352.1
    mRNA BC013157.2 None
    mRNA BC019827.1 AAH19827.1
    mRNA CR536485.1 CAG38724.1
    mRNA CR541677.1 CAG46478.1
    mRNA D49547.1 BAA08495.1
    mRNA X62421.1 CAA44287.1
    Protein Accession Links
    GenPept Link UniProtKB Link
    P25685.4 GenPept UniProtKB/Swiss-Prot:P25685
    Q6FHS4 GenPept UniProtKB/TrEMBL:Q6FHS4
    Q6FI51 GenPept UniProtKB/TrEMBL:Q6FI51
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    Additional Links

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

    Chemical Information
    Medical
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