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Med20 mediator complex subunit 20 [ Rattus norvegicus (Norway rat) ]

Gene ID: 316209, updated on 13-Apr-2024

Summary

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Symbol
Med20provided by RGD
Full Name
mediator complex subunit 20provided by RGD
Primary source
RGD:1306415
See related
Ensembl:ENSRNOG00000013852 AllianceGenome:RGD:1306415
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Rattus norvegicus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as
Trfp; Usp49
Summary
Contributes to ubiquitin protein ligase activity. Involved in protein ubiquitination. Part of ubiquitin ligase complex. Orthologous to human MED20 (mediator complex subunit 20). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in Adrenal (RPKM 145.5), Heart (RPKM 131.5) and 9 other tissues See more
Orthologs
human mouse all
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Genomic context

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See Med20 in Genome Data Viewer
Location:
9q12
Exon count:
5
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCr8 (GCF_036323735.1) 9 NC_086027.1 (20867681..20880288, complement)
RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 9 NC_051344.1 (13370146..13382756, complement)
106 previous assembly Rnor_6.0 (GCF_000001895.5) 9 NC_005108.4 (15379379..15393131, complement)

Chromosome 9 - NC_086027.1Genomic Context describing neighboring genes Neighboring gene translocase of outer mitochondrial membrane 6 Neighboring gene ubiquitin specific peptidase 49 Neighboring gene cAMP-dependent protein kinase inhibitor gamma-like Neighboring gene bystin-like Neighboring gene cyclin D3 Neighboring gene uncharacterized LOC134480552

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
  • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
  • BioProject: PRJNA238328
  • Publication: PMID 24510058
  • Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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Related articles in PubMed

  1. Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase. Brower CS, et al. Proc Natl Acad Sci U S A, 2002 Aug 6. PMID 12149480, Free PMC Article
  2. The Mediator complex and transcription regulation. Poss ZC, et al. Crit Rev Biochem Mol Biol, 2013 Nov-Dec. PMID 24088064, Free PMC Article
  3. Structural and functional organization of TRAP220, the TRAP/mediator subunit that is targeted by nuclear receptors. Malik S, et al. Mol Cell Biol, 2004 Sep. PMID 15340084, Free PMC Article
  4. Analysis of early C2C12 myogenesis identifies stably and differentially expressed transcriptional regulators whose knock-down inhibits myoblast differentiation. Rajan S, et al. Physiol Genomics, 2012 Feb 1. PMID 22147266
  5. ATAC and Mediator coactivators form a stable complex and regulate a set of non-coding RNA genes. Krebs AR, et al. EMBO Rep, 2010 Jul. PMID 20508642, Free PMC Article

See all (7) citations in PubMed

GeneRIFs: Gene References Into Functions

General gene information

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Markers

Homology

Gene Ontology Provided by RGD

Function Evidence Code Pubs
enables transcription coactivator activity IBA
Inferred from Biological aspect of Ancestor
more info
  
contributes_to ubiquitin protein ligase activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in positive regulation of DNA-templated transcription IEA
Inferred from Electronic Annotation
more info
  
involved_in protein ubiquitination IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of transcription by RNA polymerase II IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in regulation of transcription by RNA polymerase II IEA
Inferred from Electronic Annotation
more info
  
involved_in skeletal muscle cell differentiation IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within skeletal muscle cell differentiation ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
part_of core mediator complex IEA
Inferred from Electronic Annotation
more info
  
part_of core mediator complex ISO
Inferred from Sequence Orthology
more info
  
part_of mediator complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of mediator complex IEA
Inferred from Electronic Annotation
more info
  
part_of mediator complex ISO
Inferred from Sequence Orthology
more info
  
located_in nucleus ISO
Inferred from Sequence Orthology
more info
  
part_of ubiquitin ligase complex IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
mediator of RNA polymerase II transcription subunit 20
Names
Trf (TATA binding protein-related factor)-proximal protein homolog
Trf-proximal protein homolog-like
ubiquitin specific protease 49

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001013178.2 → NP_001013196.1  mediator of RNA polymerase II transcription subunit 20 isoform 1

    See identical proteins and their annotated locations for NP_001013196.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
    Source sequence(s)
    FN804985, JAXUCZ010000009
    UniProtKB/Swiss-Prot
    Q5XIE9
    UniProtKB/TrEMBL
    A0A0G2JXV5, A6JIJ2
    Related
    ENSRNOP00000018523.5, ENSRNOT00000018523.8
    Conserved Domains (1) summary
    pfam08612
    Location:1 → 198
    Med20; TATA-binding related factor (TRF) of subunit 20 of Mediator complex

  2. NM_001277367.1 → NP_001264296.1  mediator of RNA polymerase II transcription subunit 20 isoform 2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) lacks an alternate exon in the coding region, which results in a frameshift, compared to variant 1. It encodes isoform 2, which has a shorter and distinct C-terminus, compared to isoform 1.
    Source sequence(s)
    JAXUCZ010000009
    UniProtKB/TrEMBL
    A6JIJ0
    Conserved Domains (1) summary
    pfam08612
    Location:1 → 54
    Med20; TATA-binding related factor (TRF) of subunit 20 of Mediator complex

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

  1. NC_086027.1 Reference GRCr8

    Range
    20867681..20880288 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_039083483.2 → XP_038939411.1  mediator of RNA polymerase II transcription subunit 20 isoform X1

    UniProtKB/TrEMBL
    A0A0G2JXV5
    Conserved Domains (1) summary
    pfam08612
    Location:2 → 190
    Med20; TATA-binding related factor (TRF) of subunit 20 of Mediator complex
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q5XIE9.1 GenPept UniProtKB/Swiss-Prot:Q5XIE9

Gene LinkOut

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