ARHGAP35 Rho GTPase activating protein 35 [Homo sapiens] - Gene

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Summary

Official Symbol: ARHGAP35provided by HGNC
Official Full Name: Rho GTPase activating protein 35provided by HGNC
Primary source: HGNC:4591
See related: Ensembl:ENSG00000160007; HPRD:05592; MIM:605277
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: GRF-1; GRLF1; P190A; P190-A; p190RhoGAP; p190ARhoGAP; KIAA1722; MGC10745
Summary: The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq, Jul 2008]

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Genomic context

Location :: 19q13.3

Sequence :: Chromosome: 19; NC_000019.9 (47421933..47508334)

See ARHGAP35 in Epigenomics, MapViewer

Chromosome 19 - NC_000019.9 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A neutrophil- and ss2 integrin-dependent transgenic model of the effector phase of autoimmune arthritis proceeds normally in p190RhoGAP-deficient bone marrow.
Title: Neutrophil functions and autoimmune arthritis in the absence of p190RhoGAP: generation and analysis of a novel null mutation in mice.
in addition to activation of RhoGEF(s), reduction of RhoGAP (p190) is a critical mechanism by which increased RhoGTP levels are achieved in late mitosis, thereby ensuring proper cell division.
Title: Mitotic down-regulation of p190RhoGAP is required for the successful completion of cytokinesis.
Cdh1 formed a physical complex with p190 and stimulated the efficient ubiquitination of p190, both in in vitro and in vivo.
Title: The anaphase-promoting complex/cyclosome activator Cdh1 modulates Rho GTPase by targeting p190 RhoGAP for degradation.
Data show that fibroblast, endothelial and carcinoma polarity during cell migration requires FAK and is associated with a complex between FAK, p120RasGAP and p190RhoGAP (p190A), leading to p190A tyrosine phosphorylation.
Title: A FAK-p120RasGAP-p190RhoGAP complex regulates polarity in migrating cells.
p190RhoGAP and p120ctn associated predominantly on the plasma membrane of cells overexpressing E-cadherin, and that E-cadherin-bound p120ctn contributed to RhoA inactivation by favoring p190RhoGAP-RhoA association.
Title: Overexpression of E-cadherin on melanoma cells inhibits chemokine-promoted invasion involving p190RhoGAP/p120ctn-dependent inactivation of RhoA.
nmr temperature studies link the ability of p190RhoGAP protein domain FF1 to be phosphorylated with conformational changes in three-dimensional structure.
Title: NMR structural studies on human p190-A RhoGAPFF1 revealed that domain phosphorylation by the PDGF-receptor alpha requires its previous unfolding.
activation of the RhoA GTPase was defective in VHL(-) cells, and this was possibly mediated by an increased activation of its inhibitor, p190RhoGAP.
Title: Inadequate activation of the GTPase RhoA contributes to the lack of fibronectin matrix assembly in von Hippel-Lindau protein-defective renal cancer cells.
G(alpha)(13)-dependent downstream effects on RhoA activation and invasion tightly depend on cell type-specific GAP activities and that G(alpha)(13)-p190RhoGAP signaling might represent a potential target for intervention in melanoma metastasis.
Title: Activated G(alpha)13 impairs cell invasiveness through p190RhoGAP-mediated inhibition of RhoA activity.
A previously unknown function of Brk in regulating both RhoA and Ras by phosphorylating p190 and a crucial role of this Brk-elicited signaling pathway in promoting breast malignancy.
Title: Breast tumor kinase phosphorylates p190RhoGAP to regulate rho and ras and promote breast carcinoma growth, migration, and invasion.
results suggest that co-regulation of Rho activity by p190RhoGAP and ECT2 in the cleavage furrow determines whether cells properly complete cytokinesis
Title: Opposing roles of p190RhoGAP and Ect2 RhoGEF in regulating cytokinesis.

Submit: New GeneRIF Correction See all (14)

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Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

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Products	Interactant	Other Gene	Source	Pubs	Description
Q9NRY4	P23471	PTPRZ1	HPRD	PubMed
Q9NRY4	P12931	SRC	HPRD	PubMed
BioGRID:109166	BioGRID:107076	BCL6	BioGRID	PubMed	Affinity Capture-MS
BioGRID:109166	BioGRID:109142	GRB2	BioGRID	PubMed	Affinity Capture-Western
BioGRID:109166	BioGRID:109315	HDAC1	BioGRID	PubMed	FRET; Reconstituted Complex
BioGRID:109166	BioGRID:111856	RASA1	BioGRID	PubMed	Reconstituted Complex
BioGRID:109166	BioGRID:106880	RHOA	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex
BioGRID:109166	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS

Function	Evidence Code	Pubs
DNA binding	IEA Inferred from Electronic Annotation more info
GTP binding	IEA Inferred from Electronic Annotation more info
GTPase activator activity	IEA Inferred from Electronic Annotation more info
Rho GTPase activator activity	TAS Traceable Author Statement more info
transcription corepressor activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
axon guidance	TAS Traceable Author Statement more info
negative regulation of transcription, DNA-dependent	IDA Inferred from Direct Assay more info	PubMed
positive regulation of Rho GTPase activity	TAS Traceable Author Statement more info
regulation of small GTPase mediated signal transduction	TAS Traceable Author Statement more info
signal transduction	IEA Inferred from Electronic Annotation more info
small GTPase mediated signal transduction	TAS Traceable Author Statement more info
transcription, DNA-dependent	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
cytoplasm	IEA Inferred from Electronic Annotation more info
cytosol	TAS Traceable Author Statement more info
intracellular	IEA Inferred from Electronic Annotation more info
nucleus	IC Inferred by Curator more info	PubMed

General protein information

Preferred Names: rho GTPase-activating protein 35

Names: rho GTPase-activating protein 35; rho GAP p190A; glucocorticoid receptor repression factor 1; glucocorticoid receptor DNA binding factor 1; glucocorticoid receptor DNA-binding factor 1

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NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_004491.4 → NP_004482.4 rho GTPase-activating protein 35

Status: REVIEWED

Source sequence(s)

AB051509, AC008755, BE871213, CA429690, CX163309

Consensus CDS

CCDS46127.1

UniProtKB/Swiss-Prot

Q9NRY4

Related

ENSP00000385720, ENST00000404338

Conserved Domains (4) summary

cd04373
Location:1247 – 1431
Blast Score: 869

RhoGAP_p190; RhoGAP_p190: RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of p190-like proteins. p190, also named RhoGAP5, plays a role in neuritogenesis and axon branch stability. p190 shows a preference for Rho, over Rac and Cdc42, and ...

smart00324
Location:1259 – 1432
Blast Score: 536

RhoGAP; GTPase-activator protein for Rho-like GTPases

cl02610
Location:431 – 483
Blast Score: 128

FF; FF domain

cl09099
Location:158 – 247
Blast Score: 127

P-loop_NTPase; P-loop containing Nucleoside Triphosphate Hydrolases

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000019.9 Reference GRCh37.p5 Primary Assembly

Range

47421933..47508334

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000151.1 Alternate HuRef

Range

43846727..43932756

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

NM_024342.1: Suppressed sequence

Description

NM_024342.1: This RefSeq was permanently suppressed because it is a nonsense-mediated decay (NMD) candidate.

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Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AC008755.8 (22660..31277)	None
genomic	AC008895.7 (68853..146007)	None
genomic	CH471126.1	EAW57450.1
		EAW57451.1
		EAW57452.1
		EAW57453.1
mRNA	AB051509.2	BAB21813.2
mRNA	AF159851.1	AAF80386.1
mRNA	AK096123.1	None
mRNA	AK097314.1	None
mRNA	AK298356.1	BAG60600.1
mRNA	AL831914.1	None
mRNA	BC003514.1	None
mRNA	BC065491.1	None
mRNA	BC131565.1	AAI31566.1
mRNA	BC150257.1	AAI50258.1
mRNA	BE871213.1	None
mRNA	BM855708.1	None
mRNA	CA429690.1	None
mRNA	CX163309.1	None
mRNA	M73077.1	AAA58618.1