PRPF19 PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae) [Homo sapiens] - Gene

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Summary

Official Symbol: PRPF19provided by HGNC
Official Full Name: PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae)provided by HGNC
Primary source: HGNC:17896
See related: Ensembl:ENSG00000110107; HPRD:12214; MIM:608330; Vega:OTTHUMG00000167798
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: PSO4; SNEV; PRP19; UBOX4; hPSO4; NMP200
Summary: PSO4 is the human homolog of yeast Pso4, a gene essential for cell survival and DNA repair (Beck et al., 2008 [PubMed 18263876]).[supplied by OMIM, Sep 2008]

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Genomic context

Location :: 11q12.2

Sequence :: Chromosome: 11; NC_000011.9 (60658019..60674061, complement)

See PRPF19 in Epigenomics, MapViewer

Chromosome 11 - NC_000011.9 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

U2AF65 binds directly to the phosphorylated C-terminal domain, and that this interaction results in increased recruitment of U2AF65 and PRP19C to the pre-mRNA
Title: The RNA polymerase II C-terminal domain promotes splicing activation through recruitment of a U2AF65-Prp19 complex.
hPso4, once it forms a complex with Metnase, negatively regulates Metnase's TIR binding activity
Title: Regulation of Metnase's TIR binding activity by its binding partner, Pso4.
Purified hPrp19/CDC5L complexes exhibit an elongated, asymmetric shape with a maximum dimension of approximately 20 nm.
Title: Molecular architecture of the human Prp19/CDC5L complex.
Blom7alpha is a novel splicing factor of the K homology domain family that might be implicated in alternative splicing by helping to position the CDC5L-SNEV(Prp19-Pso4) complex at the splice sites [Blom7alpha]
Title: Blom7alpha is a novel heterogeneous nuclear ribonucleoprotein K homology domain protein involved in pre-mRNA splicing that interacts with SNEVPrp19-Pso4.
hPso4 is necessary to bring Metnase to the DSB sites for its function(s) in DNA repair
Title: Human Pso4 is a metnase (SETMAR)-binding partner that regulates metnase function in DNA repair.
SNEV, the human orthologue of yeast PRP19, functions in splicing and homo-oligomerization of SNEV in HeLa nuclear extract is essential for spliceosome assembly.
Title: SNEV is an evolutionarily conserved splicing factor whose oligomerization is necessary for spliceosome assembly.
The high levels of SNEV might extend the cellular life span by increasing the resistance to stress or by improving the DNA repair capacity of the cells.
Title: SNEV overexpression extends the life span of human endothelial cells.
required prior to catalytic step 1 of splicing, but not for stable integration of U4/U6.U5 tri-snRNPs
Title: A subset of human 35S U5 proteins, including Prp19, function prior to catalytic step 1 of splicing.
report the identification of two additional factors required in the DNA interstrand cross-links repair process, a previously characterized pre-mRNA splicing complex composed of Pso4/Prp19 and Werner syndrome protein(WRN)
Title: The Pso4 mRNA splicing and DNA repair complex interacts with WRN for processing of DNA interstrand cross-links.
ubiquitylated hPrp19 fails to interact with either Cdc5L or Plrg1 indicating that DNA damage can induce profound alterations to hPrp19 core complex.
Title: The Prp19/Pso4 core complex undergoes ubiquitylation and structural alterations in response to DNA damage.

Submit: New GeneRIF Correction See all (11)

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Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

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Products	Interactant	Other Gene	Source	Pubs	Description
Q9UMS4	P04053	DNTT	HPRD	PubMed
Q9UMS4	Q9UPT5	EXOC7	HPRD	PubMed
BioGRID:118151	BioGRID:107424	CDC5L	BioGRID	PubMed	Affinity Capture-MS; Co-purification; Reconstituted Complex
BioGRID:118151	BioGRID:121123	CTNNBL1	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western
BioGRID:118151	BioGRID:214866	Cdc5l	BioGRID	PubMed	Affinity Capture-MS
BioGRID:118151	BioGRID:108127	DNTT	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western
BioGRID:118151	BioGRID:125184	EGLN2	BioGRID	PubMed	Affinity Capture-Western
BioGRID:118151	BioGRID:125185	EGLN3	BioGRID	PubMed	Affinity Capture-Western; FRET; Reconstituted Complex
BioGRID:118151	BioGRID:116867	EXOC7	BioGRID	PubMed	Two-hybrid
BioGRID:118151	BioGRID:115331	HDAC5	BioGRID	PubMed	Affinity Capture-MS
BioGRID:118151	BioGRID:110034	KPNA1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:118151	BioGRID:110036	KPNA2	BioGRID	PubMed	Affinity Capture-Western
BioGRID:118151	BioGRID:111370	PLRG1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:118151	BioGRID:118151	PRPF19	BioGRID	PubMed	Affinity Capture-Western
BioGRID:118151	BioGRID:114577	PRPF3	BioGRID	PubMed	Affinity Capture-Western; Biochemical Activity; Reconstituted Complex
BioGRID:118151	BioGRID:111657	PSMA3	BioGRID	PubMed	Affinity Capture-MS
BioGRID:118151	BioGRID:228650	Prpf8	BioGRID	PubMed	Affinity Capture-MS
BioGRID:118151	BioGRID:112317	SETMAR	BioGRID	PubMed	Affinity Capture-Western
BioGRID:118151	BioGRID:121244	SMARCAD1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:118151	BioGRID:115544	SRRM1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:118151	BioGRID:117071	SRRM2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:118151	BioGRID:113188	SUMO1	BioGRID	PubMed	Reconstituted Complex
BioGRID:118151	BioGRID:211406	Snw1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:118151	BioGRID:112734	TADA2A	BioGRID	PubMed	Affinity Capture-MS
BioGRID:118151	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS
BioGRID:118151	BioGRID:113364	YWHAG	BioGRID	PubMed	Affinity Capture-MS

Function	Evidence Code	Pubs
DNA binding	IEA Inferred from Electronic Annotation more info
identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
protein binding	IPI Inferred from Physical Interaction more info	PubMed
ubiquitin-protein ligase activity	IDA Inferred from Direct Assay more info	PubMed
ubiquitin-ubiquitin ligase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
DNA repair	IEA Inferred from Electronic Annotation more info
RNA splicing	IEA Inferred from Electronic Annotation more info
inner cell mass cell proliferation	IEA Inferred from Electronic Annotation more info
lipid biosynthetic process	IEA Inferred from Electronic Annotation more info
negative regulation of neuron differentiation	IEA Inferred from Electronic Annotation more info
nuclear mRNA splicing, via spliceosome	IC Inferred by Curator more info	PubMed
positive regulation of astrocyte differentiation	IEA Inferred from Electronic Annotation more info
protein polyubiquitination	IDA Inferred from Direct Assay more info	PubMed
spliceosome assembly	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
catalytic step 2 spliceosome	IDA Inferred from Direct Assay more info	PubMed
cytoplasm	IDA Inferred from Direct Assay more info	PubMed
cytoskeleton	IEA Inferred from Electronic Annotation more info
lipid particle	IEA Inferred from Electronic Annotation more info
nuclear speck	IDA Inferred from Direct Assay more info	PubMed
nucleoplasm	IEA Inferred from Electronic Annotation more info
nucleus	IDA Inferred from Direct Assay more info	PubMed
spindle	IEA Inferred from Electronic Annotation more info
ubiquitin ligase complex	IEA Inferred from Electronic Annotation more info

General protein information

Preferred Names: pre-mRNA-processing factor 19

Names: pre-mRNA-processing factor 19; PRP19/PSO4 homolog; senescence evasion factor; nuclear matrix protein 200; nuclear matrix protein NMP200 related to splicing factor PRP19

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NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_014502.4 → NP_055317.1 pre-mRNA-processing factor 19

Status: VALIDATED

Source sequence(s)

AL555169, AP000777, AP003721, AW673738

Consensus CDS

CCDS7995.1

UniProtKB/Swiss-Prot

Q9UMS4

Related

ENSP00000227524, OTTHUMP00000236892, ENST00000227524, OTTHUMT00000396334

Conserved Domains (3) summary

cd00200
Location:223 – 503
Blast Score: 476

WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...

pfam08606
Location:66 – 135
Blast Score: 268

Prp19; Prp19/Pso4-like

cl15348
Location:2 – 68
Blast Score: 205

RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000011.9 Reference GRCh37.p5 Primary Assembly

Range

60658019..60674061, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000143.1 Alternate HuRef

Range

57004177..57020231, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

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Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AP000777.4 (118399..121416)	None
genomic	AP003721.3	None
genomic	CH471076.1	EAW73904.1
		EAW73905.1
mRNA	AF086469.1	None
mRNA	AJ131186.1	CAB51857.1
mRNA	AL555169.3	None
mRNA	AW673738.1	None
mRNA	BC008719.2	AAH08719.1
mRNA	BC018665.2	AAH18665.1
mRNA	BC018698.2	AAH18698.1
other-genetic	DQ893003.2	ABM83929.1
other-genetic	DQ896249.2	ABM87248.1