TNRC6A trinucleotide repeat containing 6A [Homo sapiens] - Gene

Help top of page

Summary

Official Symbol: TNRC6Aprovided by HGNC
Official Full Name: trinucleotide repeat containing 6Aprovided by HGNC
Primary source: HGNC:11969
See related: Ensembl:ENSG00000090905; HPRD:11640; MIM:610739; Vega:OTTHUMG00000096999
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: GW1; GW182; TNRC6; CAGH26; FLJ22043; KIAA1460; MGC75384; DKFZp666E117
Summary: This gene encodes a member of the trinucleotide repeat containing 6 protein family. The protein functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. The protein associates with messenger RNAs and Argonaute proteins in cytoplasmic bodies known as GW-bodies or P-bodies. Inhibiting expression of this gene delocalizes other GW-body proteins and impairs RNAi and microRNA-induced gene silencing. [provided by RefSeq, Jul 2008]

Help top of page

Genomic context

Location :: 16p11.2

Sequence :: Chromosome: 16; NC_000016.9 (24741049..24837548)

See TNRC6A in Epigenomics, MapViewer

Chromosome 16 - NC_000016.9 Genomic Context describing neighboring genes

Help top of page

Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

Help top of page

Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that GW182 serves as both a platform that recruits deadenylases and as a deadenylase coactivator that facilitates the removal of the poly(A) tail by CCR4-NOT.
Title: miRNA-mediated deadenylation is orchestrated by GW182 through two conserved motifs that interact with CCR4-NOT.
Data demonstrate that in human and Drosophila melanogaster cells, the critical repressive features of both the N-terminal and C-terminal effector domains of GW182 proteins are Gly/Ser/Thr-Trp (G/S/TW) or Trp-Gly/Ser/Thr (WG/S/T) motifs.
Title: miRNA repression involves GW182-mediated recruitment of CCR4-NOT through conserved W-containing motifs.
Alanine substitution showed that GW/WG motifs in Delta12 (Delta12a, amino acids 896-1045) were important for the silencing activity of GW182.
Title: Divergent GW182 functional domains in the regulation of translational silencing.
data indicate that frameshift mutations in AGO2 and TNRC6A and their losses of expression are common in GCs and CRCs with MSI-H, and suggest that these alterations may contribute to the cancer development by deregulating miRNA regulation.
Title: Somatic mutations and losses of expression of microRNA regulation-related genes AGO2 and TNRC6A in gastric and colorectal cancers.
Structural basis of binding of P-body-associated proteins GW182 and ataxin-2 by the Mlle domain of poly(A)-binding protein.(
Title: Structural basis of binding of P-body-associated proteins GW182 and ataxin-2 by the Mlle domain of poly(A)-binding protein.
over-expression of Ago2 and TNRC6A may be related to miRNA functions and might play a role in tumorigenesis of prostate carcinomas and esophageal squamous cell carcinomas.
Title: Immunohistochemical analysis of RNA-induced silencing complex-related proteins AGO2 and TNRC6A in prostate and esophageal cancers.
Our findings indicate that TNRC6A, is recruited to miRNA targets through an interaction between their N-terminal domain and an Argonaute protein
Title: The C-terminal domains of human TNRC6A, TNRC6B, and TNRC6C silence bound transcripts independently of Argonaute proteins.
Tethering the C-terminal half of Ago2 to the 3'-UTR of reporter mRNA recapitulated translational repression comparable to that of tethered Ago2, and this repression was greatly impaired upon GW182 knockdown.
Title: The C-terminal half of human Ago2 binds to multiple GW-rich regions of GW182 and requires GW182 to mediate silencing.
Data show that siRNA transfection induces up-regulated expression of both GW182, a key P-body component, and Ago2, indicating that P-body localization and interaction with GW182 and Ago2 are important in siRNA-mediated RNAi.
Title: Localization of double-stranded small interfering RNA to cytoplasmic processing bodies is Ago2 dependent and results in up-regulation of GW182 and Argonaute-2.
A novel 210-kDa isoform of human GW182, provisionally named trinucleotide GW1 (TNGW1)was reported.TNGW1 was expressed independently of GW182 and was present in human testis and various human cancer cells.
Title: Identification of GW182 and its novel isoform TNGW1 as translational repressors in Ago2-mediated silencing.

Submit: New GeneRIF Correction See all (15)

Help top of page

Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

Help top of page

Products	Interactant	Other Gene	Source	Pubs	Description
BioGRID:118141	BioGRID:2598	AGO4	BioGRID	PubMed	Reconstituted Complex
BioGRID:118141	BioGRID:118041	EIF2C2	BioGRID	PubMed	Reconstituted Complex
BioGRID:118141	BioGRID:128178	EIF2C4	BioGRID	PubMed	Affinity Capture-MS
BioGRID:118141	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS
BioGRID:118141	BioGRID:122403	WNK1	BioGRID	PubMed	Two-hybrid

Function	Evidence Code	Pubs
RNA binding	IEA Inferred from Electronic Annotation more info
nucleotide binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
cellular response to starvation	IEA Inferred from Electronic Annotation more info
gene silencing by RNA	IEA Inferred from Electronic Annotation more info
negative regulation of translation involved in gene silencing by miRNA	IDA Inferred from Direct Assay more info	PubMed
regulation of translation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
cytoplasm	IEA Inferred from Electronic Annotation more info
cytoplasmic mRNA processing body	IDA Inferred from Direct Assay more info
micro-ribonucleoprotein complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

Preferred Names: trinucleotide repeat-containing gene 6A protein

Names: trinucleotide repeat-containing gene 6A protein; EDIE; GW182 autoantigen; CAG repeat protein 26; EMSY interactor protein; glycine-tryptophan protein of 182 kDa

Help top of page

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_014494.2 → NP_055309.2 trinucleotide repeat-containing gene 6A protein

Status: REVIEWED

Source sequence(s)

AC008731, AJ492221, BE158958, BU743266

Consensus CDS

CCDS10624.2

UniProtKB/Swiss-Prot

Q8NDV7

Related

ENSP00000379144, OTTHUMP00000122485, ENST00000395799, OTTHUMT00000214081

Conserved Domains (2) summary

cd00590
Location:1783 – 1850
Blast Score: 114

RRM; RRM (RNA recognition motif), also known as RBD (RNA binding domain) or RNP (ribonucleoprotein domain), is a highly abundant domain in eukaryotes found in proteins involved in post-transcriptional gene expression processes including mRNA and rRNA ...

pfam10427
Location:1075 – 1152
Blast Score: 130

Ago_hook; Argonaute hook

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000016.9 Reference GRCh37.p5 Primary Assembly

Range

24741049..24837548

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000148.1 Alternate HuRef

Range

22832154..22928155

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Help top of page

Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AC002565.1	None
genomic	AC008731.8 (112135..188371)	None
genomic	CH471145.2	EAW55782.1
		EAW55783.1
		EAW55784.1
		EAW55785.1
		EAW55786.1
		EAW55787.1
mRNA	AB040893.1	BAA95984.1
mRNA	AJ492221.1	CAD37348.1
mRNA	AK001773.1	BAA91899.1
mRNA	AK025277.1	None
mRNA	AK025696.1	None
mRNA	AK094542.1	BAG52884.1
mRNA	AK299905.1	BAG61746.1
mRNA	AL713750.1	CAD28525.2
mRNA	AL833757.1	CAH56236.1
mRNA	AY035864.1	AAK62026.1
mRNA	BC024324.1	AAH24324.2
mRNA	BC068209.1	None
mRNA	BE158958.1	None
mRNA	BU743266.1	None
mRNA	U23860.1	None
mRNA	U80739.1	AAB91438.1
other-genetic	BC172409.1	AAI72409.1