RL2 contains a RING finger; disrupts ND10; proteasome-dependent degradation of several cellular proteins [Human herpesvirus 1] - Gene

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Summary

Gene symbol: RL2
Gene description: contains a RING finger; disrupts ND10; proteasome-dependent degradation of several cellular proteins
Locus tag: HHV1gp082
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Human herpesvirus 1 (strain: 17, acronym: HHV-1, acronym: HSV-1, nat-host: Homo sapiens, gb-synonym: Herpes simplex virus 1)
Lineage: Viruses; dsDNA viruses, no RNA stage; Herpesvirales; Herpesviridae; Alphaherpesvirinae; Simplexvirus

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Genomic context

Sequence :: NC_001806.1 (120673..124285, complement)

NC_001806.1 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The authors show biochemically that UL7 and UL23 are indeed mature virion tegument components that, unlike ICP0 and ICP4, are salt-extractable.
Title: Biochemical analysis of infected cell polypeptide (ICP)0, ICP4, UL7 and UL23 incorporated into extracellular herpes simplex virus type 1 virions.
In the early phase of infection, HSV ICP0 interacts with tankyrase 1 and mediates its nuclear translocation.
Title: Herpes simplex virus requires poly(ADP-ribose) polymerase activity for efficient replication and induces extracellular signal-related kinase-dependent phosphorylation and ICP0-dependent nuclear localization of tankyrase 1.
Herpes simplex virus 1 ICP0 phosphorylation site mutants are attenuated for viral replication and impaired for explant-induced reactivation.
Title: Herpes simplex virus 1 ICP0 phosphorylation site mutants are attenuated for viral replication and impaired for explant-induced reactivation.
The viral protein ICP0 targets RNF8 and RNF168 for degradation, thereby preventing the deposition of repressive ubiquitin marks and counteracting this repair protein recruitment.
Title: The intrinsic antiviral defense to incoming HSV-1 genomes includes specific DNA repair proteins and is counteracted by the viral protein ICP0.
infection of SIAH-1 knockdown cells with HSV, higher levels of ICP0 were produced, ICP0 was less ubiquitinated, and the half-life of this multifunctional viral regulatory protein was increased
Title: Herpes simplex virus immediate-early protein ICP0 is targeted by SIAH-1 for proteasomal degradation.
The results suggest that INSM1 up-regulation plays a positive role in HSV-1 replication, probably by binding to the ICP0 promoter.
Title: Herpes simplex virus induces the marked up-regulation of the zinc finger transcriptional factor INSM1, which modulates the expression and localization of the immediate early protein ICP0.
We propose that proteasome-mediated degradation of a virion or host protein is regulated by ICP0 to allow efficient delivery of entering HSV capsids to the nuclear periphery.
Title: A pre-immediate-early role for tegument ICP0 in the proteasome-dependent entry of herpes simplex virus.
The results demonstrate that activities or interactions specified by both the amino- and the carboxy-terminal region of ICP0 significantly contribute to virus activation.
Title: Activities of ICP0 involved in the reversal of silencing of quiescent herpes simplex virus 1.
proteasome function is required to localize ICP0 to the cytoplasm where it mediates its inhibitory effect independent of E3 ubiquitin ligase activity
Title: Cellular localization of the herpes simplex virus ICP0 protein dictates its ability to block IRF3-mediated innate immune responses.
These results indicate that ICP0 activity ultimately affects chromatin structure of quiescent genomes at multiple levels, including higher-order chromatin structure, histone modifications, and histone association.
Title: Reversal of heterochromatic silencing of quiescent herpes simplex virus type 1 by ICP0.

Submit: New GeneRIF Correction See all (36)

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Interactions

Products	Interactant	Other Gene	Source	Pubs	Description
BioGRID:971427	BioGRID:115106	HDAC4	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex
BioGRID:971427	BioGRID:115331	HDAC5	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex
BioGRID:971427	BioGRID:115083	HDAC9	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex
BioGRID:971427	BioGRID:116796	RCOR1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:971427	BioGRID:112372	SIAH1	BioGRID	PubMed	Affinity Capture-Western; Biochemical Activity
BioGRID:971427	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex
BioGRID:971427	BioGRID:113169	UBE2D1	BioGRID	PubMed	Reconstituted Complex
BioGRID:971427	BioGRID:113172	UBE2E1	BioGRID	PubMed	Reconstituted Complex
BioGRID:971427	BioGRID:113622	USP7	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex

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General gene information

TATA_signal

Loc: c124285-124280

polyA_signal

Loc: c120695-120690

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General protein information

Preferred Names: contains a RING finger; disrupts ND10; proteasome-dependent degradation of several cellular proteins

Names: ubiquitin E3 ligase ICP0

NP_044660.1

VOG classification

Description:

VOGd0141 [T] * Ring finger domain viral protein, (putative) E3 ubiquitin ligase, some members are transcriptional regulators.
RNA Replication, Transcription and Modification

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NCBI Reference Sequences (RefSeq)

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

mRNA and Protein(s)

NP_044660.1 ubiquitin E3 ligase ICP0 [Human herpesvirus 1]

UniProtKB/TrEMBL

G8HBH2

UniProtKB/Swiss-Prot

P08393

Conserved Domains (2) summary

cd00162
Location:116 – 160
Blast Score: 115

RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...

pfam13923
Location:112 – 158
Blast Score: 119

zf-C3HC4_2; Zinc finger, C3HC4 type (RING finger)