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GAPDH glyceraldehyde-3-phosphate dehydrogenase [ Homo sapiens (human) ]

Gene ID: 2597, updated on 16-Apr-2014
Official Symbol
GAPDHprovided by HGNC
Official Full Name
glyceraldehyde-3-phosphate dehydrogenaseprovided by HGNC
Primary source
HGNC:4141
Locus tag
CDABP0047
See related
Ensembl:ENSG00000111640; HPRD:00713; MIM:138400; Vega:OTTHUMG00000137379
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
G3PD; GAPD; HEL-S-162eP
Summary
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Location :
12p13
Sequence :
Chromosome: 12; NC_000012.12 (6534419..6538371)
See GAPDH in Epigenomics, MapViewer

Chromosome 12 - NC_000012.12Genomic Context describing neighboring genes Neighboring gene mitochondrial ribosomal protein L51 Neighboring gene non-SMC condensin I complex, subunit D2 Neighboring gene small Cajal body-specific RNA 10 Neighboring gene intermediate filament family orphan 1 Neighboring gene NOP2 nucleolar protein

GeneRIFs: Gene References Into FunctionsWhat's a GeneRIF?

Protein interactions

Protein Gene Interaction Pubs
Rev, p19 rev HIV-1 Rev interacting protein, glyceraldehyde-3-phosphate dehydrogenase (GAPDH), is identified by the in-vitro binding experiments involving cytosolic or nuclear extracts from HeLa cells. The interaction of Rev with GAPDH is increased by RRE PubMed

Go to the HIV-1, Human Protein Interaction Database

  • Alzheimer's disease, organism-specific biosystem (from KEGG)
    Alzheimer's disease, organism-specific biosystemAlzheimer's disease (AD) is a chronic disorder that slowly destroys neurons and causes serious cognitive disability. AD is associated with senile plaques and neurofibrillary tangles (NFTs). Amyloid-b...
  • Alzheimer's disease, conserved biosystem (from KEGG)
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  • Alzheimers Disease, organism-specific biosystem (from WikiPathways)
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  • Carbon metabolism, conserved biosystem (from KEGG)
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  • Gluconeogenesis, organism-specific biosystem (from REACTOME)
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  • Gluconeogenesis, oxaloacetate => fructose-6P, conserved biosystem (from KEGG)
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    Glycolysis and Gluconeogenesis
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Products Interactant Other Gene Complex Source Pubs Description

Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
NAD binding IEA
Inferred from Electronic Annotation
more info
 
NADP binding IEA
Inferred from Electronic Annotation
more info
 
glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity ISS
Inferred from Sequence or Structural Similarity
more info
 
glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity NAS
Non-traceable Author Statement
more info
PubMed 
identical protein binding IPI
Inferred from Physical Interaction
more info
 
microtubule binding ISS
Inferred from Sequence or Structural Similarity
more info
 
peptidyl-cysteine S-nitrosylase activity ISS
Inferred from Sequence or Structural Similarity
more info
 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
Process Evidence Code Pubs
carbohydrate metabolic process TAS
Traceable Author Statement
more info
 
cellular response to interferon-gamma IDA
Inferred from Direct Assay
more info
PubMed 
gluconeogenesis TAS
Traceable Author Statement
more info
 
glucose metabolic process TAS
Traceable Author Statement
more info
 
glycolytic process IEA
Inferred from Electronic Annotation
more info
 
glycolytic process TAS
Traceable Author Statement
more info
 
microtubule cytoskeleton organization ISS
Inferred from Sequence or Structural Similarity
more info
 
negative regulation of translation IDA
Inferred from Direct Assay
more info
 
negative regulation of translation IMP
Inferred from Mutant Phenotype
more info
PubMed 
neuron apoptotic process ISS
Inferred from Sequence or Structural Similarity
more info
 
peptidyl-cysteine S-trans-nitrosylation ISS
Inferred from Sequence or Structural Similarity
more info
 
protein stabilization ISS
Inferred from Sequence or Structural Similarity
more info
 
small molecule metabolic process TAS
Traceable Author Statement
more info
 
Component Evidence Code Pubs
GAIT complex IDA
Inferred from Direct Assay
more info
PubMed 
cytoplasm IDA
Inferred from Direct Assay
more info
 
cytoplasm ISS
Inferred from Sequence or Structural Similarity
more info
 
cytosol ISS
Inferred from Sequence or Structural Similarity
more info
 
cytosol TAS
Traceable Author Statement
more info
 
extracellular vesicular exosome IDA
Inferred from Direct Assay
more info
PubMed 
lipid particle IDA
Inferred from Direct Assay
more info
PubMed 
microtubule cytoskeleton ISS
Inferred from Sequence or Structural Similarity
more info
 
nucleus ISS
Inferred from Sequence or Structural Similarity
more info
 
perinuclear region of cytoplasm IEA
Inferred from Electronic Annotation
more info
 
plasma membrane IDA
Inferred from Direct Assay
more info
 
ribonucleoprotein complex IDA
Inferred from Direct Assay
more info
PubMed 
Preferred Names
glyceraldehyde-3-phosphate dehydrogenase
Names
glyceraldehyde-3-phosphate dehydrogenase
aging-associated gene 9 protein
peptidyl-cysteine S-nitrosylase GAPDH
epididymis secretory sperm binding protein Li 162eP
NP_001243728.1
NP_002037.2

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

  1. NG_007073.2 RefSeqGene

    Range
    4915..8885
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001256799.2NP_001243728.1  glyceraldehyde-3-phosphate dehydrogenase isoform 2

    See proteins identical to NP_001243728.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus compared to isoform 1.
    Source sequence(s)
    AF261085, BE893087, HY000136
    Consensus CDS
    CCDS58201.1
    UniProtKB/Swiss-Prot
    P04406
    Related
    ENSP00000380067, OTTHUMP00000174434, ENST00000396858, OTTHUMT00000268066
    Conserved Domains (2) summary
    pfam00044
    Location:1110
    Blast Score: 485
    Gp_dh_N; Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain
    pfam02800
    Location:115272
    Blast Score: 737
    Gp_dh_C; Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain
  2. NM_001289745.1NP_001276674.1  glyceraldehyde-3-phosphate dehydrogenase isoform 1

    See proteins identical to NP_001276674.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 3, and 4 encode the same isoform (1).
    Source sequence(s)
    BE893087, BM763361, HY046784, M33197
    Conserved Domains (3) summary
    smart00846
    Location:4152
    Blast Score: 633
    Gp_dh_N; Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain
    COG0057
    Location:3335
    Blast Score: 1326
    GapA; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase [Carbohydrate transport and metabolism]
    pfam02800
    Location:157314
    Blast Score: 736
    Gp_dh_C; Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain
  3. NM_001289746.1NP_001276675.1  glyceraldehyde-3-phosphate dehydrogenase isoform 1

    See proteins identical to NP_001276675.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (4) differs in the 5' UTR, compared to variant 1. Variants 1, 3, and 4 encode the same isoform (1).
    Source sequence(s)
    BC023632, BE893087, HY004110, HY022295
    Conserved Domains (3) summary
    smart00846
    Location:4152
    Blast Score: 633
    Gp_dh_N; Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain
    COG0057
    Location:3335
    Blast Score: 1326
    GapA; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase [Carbohydrate transport and metabolism]
    pfam02800
    Location:157314
    Blast Score: 736
    Gp_dh_C; Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain
  4. NM_002046.5NP_002037.2  glyceraldehyde-3-phosphate dehydrogenase isoform 1

    See proteins identical to NP_002037.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) encodes the longer isoform (1).
    Source sequence(s)
    BC009081, BE893087, HY046784
    Consensus CDS
    CCDS8549.1
    UniProtKB/Swiss-Prot
    P04406
    UniProtKB/TrEMBL
    V9HVZ4
    Related
    ENSP00000380070, OTTHUMP00000174431, ENST00000396861, OTTHUMT00000268060
    Conserved Domains (3) summary
    smart00846
    Location:4152
    Blast Score: 633
    Gp_dh_N; Glyceraldehyde 3-phosphate dehydrogenase, NAD binding domain
    COG0057
    Location:3335
    Blast Score: 1326
    GapA; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase [Carbohydrate transport and metabolism]
    pfam02800
    Location:157314
    Blast Score: 736
    Gp_dh_C; Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 106

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38 Primary Assembly

Genomic

  1. NC_000012.12 Reference GRCh38 Primary Assembly

    Range
    6534419..6538371
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

  1. AC_000144.1 Alternate HuRef

    Range
    6497829..6501781
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate CHM1_1.1

Genomic

  1. NC_018923.2 Alternate CHM1_1.1

    Range
    6642592..6646544
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

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