Nos1 nitric oxide synthase 1, neuronal [Rattus norvegicus] - Gene

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Summary

Official Symbol: Nos1provided by RGD
Official Full Name: nitric oxide synthase 1, neuronalprovided by RGD
Primary source: RGD:3184
See related: Ensembl:ENSRNOG00000001130
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus
Also known as: bNOS
Summary: enzyme that catalyzes the production of nitric oxide [RGD, Feb 2006]
Annotation information: Annotation category: suggests misassembly; Annotation category: partial on reference assembly

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Genomic context

Location :: 12q16

Sequence :: Chromosome: 12; NC_005111.2 (39812500..39869484, complement)

See Nos1 in MapViewer

Chromosome 12 - NC_005111.2 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that increased nNOS production and dimerization, together with a relative decrease in catalytic activity and re-localization to mitochondria, are involved in beta cell hyperactivity in insulin-resistant rats (Zucker fa/fa rats).
Title: Increased neuronal nitric oxide synthase dimerisation is involved in rat and human pancreatic beta cell hyperactivity in obesity.
changes in the state of the heme/substrate binding cleft affect chaperone binding and thus nNOS ubiquitination.
Title: Modulation of heme/substrate binding cleft of neuronal nitric-oxide synthase (nNOS) regulates binding of Hsp90 and Hsp70 proteins and nNOS ubiquitination.
findings suggest that NO plays an important role in chronic cocaine-induced deregulation of the medial prefrontal cortex activity that may contribute to the development of behavioral sensitization and cocaine withdrawal.
Title: Inhibition of neuronal nitric oxide synthase prevents alterations in medial prefrontal cortex excitability induced by repeated cocaine administration.
Change in interstitial cells of Cajal eNOS/nNOS may contribute to the pathophysiology of bladder overactivity induced by bladder outlet obstruction.
Title: Distribution of interstitial cells of Cajal and expression of nitric oxide synthase after experimental bladder outlet obstruction in a rat model of bladder overactivity.
nNOS and CAPON co-localize in the pre-sympathetic autonomic neurons in the paraventricular nucleus and their expression is reciprocally regulated in congestive heart failure via angiotensin II.
Title: Decreased nNOS in the PVN leads to increased sympathoexcitation in chronic heart failure: role for CAPON and Ang II.
We conclude that the anteroventral third ventricle region plays a role in regulating the expression of nNOS in the supraoptic nucleus of rats
Title: Anteroventral third ventricle (AV3V) lesion affects hypothalamic neuronal nitric oxide synthase (nNOS) expression following water deprivation.
These results indicate that nNOS is more widely expressed than previously thought, being present in both inhibitory and excitatory neurons
Title: A quantitative study of neuronal nitric oxide synthase expression in laminae I-III of the rat spinal dorsal horn.
The results suggest that nitric oxide synthase-NO pathway might be involved in the CO-mediated central regulation of respiration at the level of medulla oblongata in neonatal rats.
Title: Nitric oxide synthase expression in the medullary respiratory related nuclei and its involvement in CO-mediated central respiratory effects in neonatal rats.
NOS-1-derived NO acts as a signaling element and it is essential for the initiation of systemic inflammation
Title: NOS-1-derived NO is an essential triggering signal for the development of systemic inflammatory responses.
According to our results, nNOS downregulation enhances glutamate transmission in the PVN by increasing NR(1) subunit expression. This mechanism may enhance renal sympathetic nerve activity in CHF rats.
Title: Gene transfer of neuronal nitric oxide synthase to the paraventricular nucleus reduces the enhanced glutamatergic tone in rats with chronic heart failure.

Submit: New GeneRIF Correction See all (222)

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Products	Interactant	Other Gene	Source	Pubs	Description
NP_434686.1	NP_058887.1	Adra1a	BIND	PubMed	nNOS interacts with the amino terminus of alpha-1A-AR. This interaction was modelled on a demonstrated interaction between nNOS from rat and alpha-1A-AR from human.
NP_434686.1	NP_058687.2	Adra1b	BIND	PubMed	nNOS interacts with the amino terminus of alpha-1B-AR. This interaction was modelled on a demonstrated interaction between nNOS from rat and alpha-1B-AR from human.
NP_434686.1	NP_077809.1	Adra1d	BIND	PubMed	nNOS interacts with the amino terminus of alpha-1D-AR. This interaction was modelled on a demonstrated interaction between nNOS from rat and alpha-1D-AR from human.
NP_434686.1	NP_062567.1	Dlg4	BIND	PubMed	PSD-95 interacts with nNOS.
NP_434686.1	NP_075235.2	Dlgap1	BIND	PubMed	DAP-1 interacts with nNOS.
NP_434686.1	NP_445771.1	Dynll1	BIND	PubMed	PIN interacts with nNOS.
BioGRID:246738	BioGRID:247843	Cav3	BioGRID	PubMed	Affinity Capture-Western
BioGRID:246738	BioGRID:248034	Ctbp1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:246738	BioGRID:114206	DYNLL1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:246738	BioGRID:109552	HSP90AA1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:246738	BioGRID:109540	HSPA4	BioGRID	PubMed	Affinity Capture-Western
BioGRID:246738	BioGRID:115563	STUB1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:246738	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-Western

Function	Evidence Code	Pubs
FMN binding	IDA Inferred from Direct Assay more info	PubMed
FMN binding	TAS Traceable Author Statement more info	PubMed
NADP binding	IDA Inferred from Direct Assay more info	PubMed
NADP binding	TAS Traceable Author Statement more info	PubMed
amino acid binding	TAS Traceable Author Statement more info	PubMed
cadmium ion binding	IDA Inferred from Direct Assay more info	PubMed
calmodulin binding	IDA Inferred from Direct Assay more info	PubMed
enzyme binding	IPI Inferred from Physical Interaction more info	PubMed
flavin adenine dinucleotide binding	IDA Inferred from Direct Assay more info	PubMed
flavin adenine dinucleotide binding	TAS Traceable Author Statement more info	PubMed
heme binding	IDA Inferred from Direct Assay more info	PubMed
heme binding	TAS Traceable Author Statement more info	PubMed
metal ion binding	IEA Inferred from Electronic Annotation more info
nitric-oxide synthase activity	IDA Inferred from Direct Assay more info	PubMed
nitric-oxide synthase activity	ISO Inferred from Sequence Orthology more info
nitric-oxide synthase activity	TAS Traceable Author Statement more info	PubMed
oxidoreductase activity	IEA Inferred from Electronic Annotation more info
protein binding	IPI Inferred from Physical Interaction more info	PubMed
protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
aging	IEP Inferred from Expression Pattern more info	PubMed
arginine catabolic process	IDA Inferred from Direct Assay more info	PubMed
cellular response to growth factor stimulus	ISO Inferred from Sequence Orthology more info
exogenous drug catabolic process	ISO Inferred from Sequence Orthology more info
female pregnancy	IEP Inferred from Expression Pattern more info
multicellular organismal response to stress	ISO Inferred from Sequence Orthology more info
negative regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info
negative regulation of blood pressure	IMP Inferred from Mutant Phenotype more info	PubMed
negative regulation of calcium ion transport	ISO Inferred from Sequence Orthology more info
negative regulation of cell proliferation	IMP Inferred from Mutant Phenotype more info
negative regulation of heart contraction	IMP Inferred from Mutant Phenotype more info	PubMed
negative regulation of hydrolase activity	ISO Inferred from Sequence Orthology more info
negative regulation of insulin secretion	IMP Inferred from Mutant Phenotype more info	PubMed
negative regulation of potassium ion transport	ISO Inferred from Sequence Orthology more info
negative regulation of serotonin uptake	ISO Inferred from Sequence Orthology more info
nitric oxide biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
nitric oxide biosynthetic process	ISO Inferred from Sequence Orthology more info
oxidation-reduction process	ISO Inferred from Sequence Orthology more info
peptidyl-cysteine S-nitrosylation	ISO Inferred from Sequence Orthology more info
positive regulation of histone acetylation	ISO Inferred from Sequence Orthology more info
positive regulation of transcription from RNA polymerase II promoter	ISO Inferred from Sequence Orthology more info
positive regulation of transcription, DNA-dependent	ISO Inferred from Sequence Orthology more info
positive regulation of vasodilation	IMP Inferred from Mutant Phenotype more info	PubMed
positive regulation of vasodilation	ISO Inferred from Sequence Orthology more info
regulation of heart contraction	IMP Inferred from Mutant Phenotype more info	PubMed
regulation of sensory perception of pain	IMP Inferred from Mutant Phenotype more info	PubMed
regulation of sodium ion transport	ISO Inferred from Sequence Orthology more info
response to activity	IEP Inferred from Expression Pattern more info	PubMed
response to estrogen stimulus	IDA Inferred from Direct Assay more info	PubMed
response to ethanol	IEP Inferred from Expression Pattern more info	PubMed
response to heat	IEP Inferred from Expression Pattern more info	PubMed
response to heat	ISO Inferred from Sequence Orthology more info
response to hypoxia	IEP Inferred from Expression Pattern more info	PubMed
response to hypoxia	ISO Inferred from Sequence Orthology more info
response to lead ion	IEP Inferred from Expression Pattern more info	PubMed
response to lipopolysaccharide	IEP Inferred from Expression Pattern more info
response to nicotine	IEP Inferred from Expression Pattern more info
response to nitric oxide	IEP Inferred from Expression Pattern more info
response to nutrient levels	IEP Inferred from Expression Pattern more info
response to organic cyclic compound	IEP Inferred from Expression Pattern more info	PubMed
response to organic nitrogen	IEP Inferred from Expression Pattern more info	PubMed
response to peptide hormone stimulus	IMP Inferred from Mutant Phenotype more info
response to vitamin E	IEP Inferred from Expression Pattern more info	PubMed
striated muscle contraction	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
azurophil granule	IDA Inferred from Direct Assay more info	PubMed
cell projection	IEA Inferred from Electronic Annotation more info
cytoplasm	IDA Inferred from Direct Assay more info	PubMed
cytoskeleton	ISO Inferred from Sequence Orthology more info
cytosol	IDA Inferred from Direct Assay more info	PubMed
dendrite	IDA Inferred from Direct Assay more info	PubMed
dendritic spine	IEA Inferred from Electronic Annotation more info
membrane fraction	IDA Inferred from Direct Assay more info
mitochondrial outer membrane	IDA Inferred from Direct Assay more info	PubMed
mitochondrion	IDA Inferred from Direct Assay more info	PubMed
nuclear membrane	IDA Inferred from Direct Assay more info	PubMed
nucleus	IDA Inferred from Direct Assay more info	PubMed
perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed
photoreceptor inner segment	IDA Inferred from Direct Assay more info	PubMed
plasma membrane	IDA Inferred from Direct Assay more info	PubMed
sarcolemma	IDA Inferred from Direct Assay more info	PubMed
sarcolemma	ISO Inferred from Sequence Orthology more info
sarcoplasmic reticulum	ISO Inferred from Sequence Orthology more info
soluble fraction	IDA Inferred from Direct Assay more info
synapse	IDA Inferred from Direct Assay more info	PubMed
synapse	ISO Inferred from Sequence Orthology more info
vesicle membrane	IDA Inferred from Direct Assay more info

General protein information

Preferred Names: nitric oxide synthase, brain

Names: nitric oxide synthase, brain; nNOS; N-NOS; NC-NOS; NOS type I; neuronal NOS; constitutive NOS; nitric oxidase synthase; peptidyl-cysteine S-nitrosylase NOS1

NP_434686.1

EC 1.14.13.39

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NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_052799.1 → NP_434686.1 nitric oxide synthase, brain

Status: PROVISIONAL

Source sequence(s)

U67309

UniProtKB/Swiss-Prot

P29476

Related

ENSRNOP00000062735, ENSRNOT00000066810

Conserved Domains (5) summary

cd00992
Location:15 – 98
Blast Score: 173

PDZ_signaling; PDZ domain found in a variety of Eumetazoan signaling molecules, often in tandem arrangements. May be responsible for specific protein-protein interactions, as most PDZ domains bind C-terminal polypeptides, and binding to internal (non-C-terminal) ...

cd00795
Location:300 – 711
Blast Score: 2136

NOS_oxygenase_euk; Nitric oxide synthase (NOS) eukaryotic oxygenase domain. NOS produces nitric oxide (NO) by catalyzing a five-electron heme-based oxidation of a guanidine nitrogen of L-arginine to L-citrulline via two successive monooxygenation reactions producing N ...

cd06202
Location:1030 – 1433
Blast Score: 1958

Nitric_oxide_synthase; The ferredoxin-reductase (FNR) like C-terminal domain of the nitric oxide synthase (NOS) fuses with a heme-containing N-terminal oxidase domain. The reductase portion is similar in structure to NADPH dependent cytochrome-450 reductase (CYPOR), having an ...

pfam00667
Location:1020 – 1248
Blast Score: 711

FAD_binding_1; FAD binding domain

cl00438
Location:906 – 964
Blast Score: 189

FMN_red; NADPH-dependent FMN reductase

RefSeqs of Annotated Genomes: Build 4.2

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly (based on RGSC_v3.4)

Genomic

NC_005111.2 Reference assembly (based on RGSC_v3.4)

Range

39812500..39869484, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate assembly (Rn_Celera)

Genomic

AC_000080.1 Alternate assembly (Rn_Celera)

Range

40283655..40368674, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

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Related Sequences

Nucleotide		Protein	Strain
Heading	Accession and Version	Protein	Strain
genomic	AJ001146.1	None	W
genomic	AJ005845.1	CAA06740.1
genomic	U14522.1	None	SR
mRNA	U67309.1	AAC52782.1	F344
mRNA	X59949.1	CAA42574.1