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Ago1 argonaute RISC catalytic subunit 1 [ Mus musculus (house mouse) ]

Gene ID: 236511, updated on 28-May-2016
Official Symbol
Ago1provided by MGI
Official Full Name
argonaute RISC catalytic subunit 1provided by MGI
Primary source
MGI:MGI:2446630
See related
Ensembl:ENSMUSG00000041530 Vega:OTTMUSG00000009219
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Eif2c1
Summary
This gene encodes a member of the argonaute family of proteins, which associate with small RNAs and have important roles in RNA interference (RNAi) and RNA silencing. This protein binds to microRNAs (miRNAs) or small interfering RNAs (siRNAs) and represses translation of mRNAs that are complementary to them. It is also involved in transcriptional gene silencing (TGS) of promoter regions that are complementary to bound short antigene RNAs (agRNAs), as well as in the degradation of miRNA-bound mRNA targets. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A recent study showed this gene to be an authentic stop codon readthrough target, and that its mRNA could give rise to an additional C-terminally extended isoform by use of an alternative in-frame translation termination codon. [provided by RefSeq, Nov 2015]
Orthologs
Location:
4; 4 D2.2
Exon count:
19
Annotation release Status Assembly Chr Location
105 current GRCm38.p3 (GCF_000001635.23) 4 NC_000070.6 (126435012..126468709, complement)
Build 37.2 previous assembly MGSCv37 (GCF_000001635.18) 4 NC_000070.5 (126112256..126145665, complement)

Chromosome 4 - NC_000070.6Genomic Context describing neighboring genes Neighboring gene tektin 2 Neighboring gene argonaute RISC catalytic subunit 3 Neighboring gene argonaute RISC catalytic subunit 4 Neighboring gene microRNA 7119 Neighboring gene claspin

Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
Products Interactant Other Gene Complex Source Pubs Description

Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
RNA binding IDA
Inferred from Direct Assay
more info
PubMed 
RNA binding ISO
Inferred from Sequence Orthology
more info
 
RNA polymerase II core binding ISO
Inferred from Sequence Orthology
more info
 
RNA polymerase II core promoter proximal region sequence-specific DNA binding IDA
Inferred from Direct Assay
more info
PubMed 
core promoter binding ISO
Inferred from Sequence Orthology
more info
 
double-stranded RNA binding ISO
Inferred from Sequence Orthology
more info
 
miRNA binding IDA
Inferred from Direct Assay
more info
PubMed 
miRNA binding ISO
Inferred from Sequence Orthology
more info
 
nucleic acid binding IEA
Inferred from Electronic Annotation
more info
 
poly(A) RNA binding ISO
Inferred from Sequence Orthology
more info
 
protein binding IPI
Inferred from Physical Interaction
more info
PubMed 
single-stranded RNA binding ISO
Inferred from Sequence Orthology
more info
 
transcription regulatory region DNA binding IMP
Inferred from Mutant Phenotype
more info
PubMed 
Process Evidence Code Pubs
RNA secondary structure unwinding ISO
Inferred from Sequence Orthology
more info
 
gene silencing by RNA IEA
Inferred from Electronic Annotation
more info
 
miRNA loading onto RISC involved in gene silencing by miRNA ISO
Inferred from Sequence Orthology
more info
 
miRNA mediated inhibition of translation ISO
Inferred from Sequence Orthology
more info
 
miRNA metabolic process IDA
Inferred from Direct Assay
more info
PubMed 
nuclear-transcribed mRNA catabolic process ISO
Inferred from Sequence Orthology
more info
 
positive regulation of gene expression IMP
Inferred from Mutant Phenotype
more info
PubMed 
positive regulation of transcription from RNA polymerase II promoter IDA
Inferred from Direct Assay
more info
PubMed 
positive regulation of transcription from RNA polymerase II promoter IMP
Inferred from Mutant Phenotype
more info
PubMed 
positive regulation of transcription from RNA polymerase II promoter ISO
Inferred from Sequence Orthology
more info
 
pre-miRNA processing ISO
Inferred from Sequence Orthology
more info
 
production of miRNAs involved in gene silencing by miRNA ISO
Inferred from Sequence Orthology
more info
 
regulation of transcription, DNA-templated IEA
Inferred from Electronic Annotation
more info
 
regulation of translation IEA
Inferred from Electronic Annotation
more info
 
transcription, DNA-templated IEA
Inferred from Electronic Annotation
more info
 
Component Evidence Code Pubs
RISC complex ISO
Inferred from Sequence Orthology
more info
 
RISC-loading complex ISO
Inferred from Sequence Orthology
more info
 
cytoplasm ISO
Inferred from Sequence Orthology
more info
PubMed 
cytoplasmic mRNA processing body IDA
Inferred from Direct Assay
more info
PubMed 
cytosol IDA
Inferred from Direct Assay
more info
PubMed 
intracellular ribonucleoprotein complex ISO
Inferred from Sequence Orthology
more info
 
micro-ribonucleoprotein complex IDA
Inferred from Direct Assay
more info
PubMed 
micro-ribonucleoprotein complex ISO
Inferred from Sequence Orthology
more info
 
nucleus IDA
Inferred from Direct Assay
more info
PubMed 
polysome ISO
Inferred from Sequence Orthology
more info
 
Preferred Names
protein argonaute-1
Names
Piwi/Argonaute family protein meIF2C1
argonaute 1
argonaute RISC catalytic component 1
argonaute1
eIF-2C 1
eIF2C 1
eukaryotic translation initiation factor 2C, 1
mAgo1

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001317173.1NP_001304102.1  protein argonaute-1 isoform 1x

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon (nt 2734-2736), while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UAG termination codon (nt 2848-2850). This RefSeq represents the longer, C-terminally extended isoform. As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), its location in the longer isoform is denoted by an 'X'.
    Source sequence(s)
    AI451331, AK080954, AL606976, BC129914, CJ130124, CV559803
    UniProtKB/Swiss-Prot
    Q8CJG1
    UniProtKB/TrEMBL
    Q8BJP2
    Conserved Domains (5) summary
    PLN03202
    Location:26856
    PLN03202; protein argonaute; Provisional
    cd02846
    Location:225345
    PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...
    cd04657
    Location:390815
    Piwi_ago-like; Piwi_ago-like: PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of ...
    pfam08699
    Location:173225
    DUF1785; Domain of unknown function (DUF1785)
    cl10015
    Location:51117
    YjgF_YER057c_UK114_family; YjgF, YER057c, and UK114 belong to a large family of proteins present in bacteria, archaea, and eukaryotes with no definitive function. The conserved domain is similar in structure to chorismate mutase but there is no sequence similarity and no ...
  2. NM_001317174.1NP_001304103.1  protein argonaute-1 isoform 2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) uses an alternate acceptor splice site in the 5' coding region and initiates translation from an alternate start codon compared to variant 1. The resulting isoform (2) is shorter with a distinct N-terminus compared to isoform 1.
    Source sequence(s)
    AI451331, AK080954, AL606976, CJ130124, CV559803
    UniProtKB/TrEMBL
    Q8BJP2
    Conserved Domains (3) summary
    cd02846
    Location:74194
    PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...
    cd04657
    Location:239664
    Piwi_ago-like; Piwi_ago-like: PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of ...
    pfam08699
    Location:2274
    DUF1785; Domain of unknown function (DUF1785)
  3. NM_153403.3NP_700452.2  protein argonaute-1 isoform 1

    See identical proteins and their annotated locations for NP_700452.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) represents the predominant transcript and encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (1) results from translation termination at the upstream UGA stop codon (nt 2734-2736), while the longer isoform (1x) results from UGA stop codon readthrough to the downstream UAG termination codon (nt 2848-2850). This RefSeq represents the shorter isoform.
    Source sequence(s)
    AI451331, AK080954, AL606976, BC129914, CJ130124, CV559803
    Consensus CDS
    CCDS18653.1
    UniProtKB/Swiss-Prot
    Q8CJG1
    UniProtKB/TrEMBL
    Q8BJP2
    Related
    ENSMUSP00000095498, OTTMUSP00000009563, ENSMUST00000097888, OTTMUST00000020859
    Conserved Domains (5) summary
    PLN03202
    Location:26856
    PLN03202; protein argonaute; Provisional
    cd02846
    Location:225345
    PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...
    cd04657
    Location:390815
    Piwi_ago-like; Piwi_ago-like: PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of ...
    pfam08699
    Location:173225
    DUF1785; Domain of unknown function (DUF1785)
    cl10015
    Location:51117
    YjgF_YER057c_UK114_family; YjgF, YER057c, and UK114 belong to a large family of proteins present in bacteria, archaea, and eukaryotes with no definitive function. The conserved domain is similar in structure to chorismate mutase but there is no sequence similarity and no ...

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 105 details...

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm38.p3 C57BL/6J

Genomic

  1. NC_000070.6 Reference GRCm38.p3 C57BL/6J

    Range
    126435012..126468709 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006503067.1XP_006503130.1  

    Conserved Domains (3) summary
    cd02846
    Location:74194
    PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...
    cd04657
    Location:239664
    Piwi_ago-like; Piwi_ago-like: PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of ...
    pfam08699
    Location:2274
    DUF1785; Domain of unknown function (DUF1785)
  2. XM_011240521.1XP_011238823.1  

    See identical proteins and their annotated locations for XP_011238823.1

    Conserved Domains (3) summary
    cd02846
    Location:74194
    PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...
    cd04657
    Location:239667
    Piwi_ago-like; Piwi_ago-like: PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of ...
    pfam08699
    Location:2274
    DUF1785; Domain of unknown function (DUF1785)
  3. XM_011240520.1XP_011238822.1  

    See identical proteins and their annotated locations for XP_011238822.1

    Conserved Domains (5) summary
    PLN03202
    Location:26859
    PLN03202; protein argonaute; Provisional
    cd02846
    Location:225345
    PAZ_argonaute_like; PAZ domain, argonaute_like subfamily. Argonaute is part of the RNA-induced silencing complex (RISC), and is an endonuclease that plays a key role in the RNA interference pathway. The PAZ domain has been named after the proteins Piwi,Argonaut, and Zwille. ...
    cd04657
    Location:390818
    Piwi_ago-like; Piwi_ago-like: PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of ...
    pfam08699
    Location:173225
    DUF1785; Domain of unknown function (DUF1785)
    cl10015
    Location:51117
    YjgF_YER057c_UK114_family; YjgF, YER057c, and UK114 belong to a large family of proteins present in bacteria, archaea, and eukaryotes with no definitive function. The conserved domain is similar in structure to chorismate mutase but there is no sequence similarity and no ...

Alternate Mm_Celera

Genomic

  1. AC_000026.1 Alternate Mm_Celera

    Range
    124771815..124805219 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)