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    POLA2 polymerase (DNA directed), alpha 2 (70kD subunit) [ Homo sapiens ]

    Gene ID: 23649, updated on 4-Feb-2012

    Summary

    Official Symbol
    POLA2provided by HGNC
    Official Full Name
    polymerase (DNA directed), alpha 2 (70kD subunit)provided by HGNC
    Primary source
    HGNC:30073
    See related
    Ensembl:ENSG00000014138; HPRD:10160; Vega:OTTHUMG00000165951
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo

    Genomic context

    Location :
    11q13.1
    Sequence :
    Chromosome: 11; NC_000011.9 (65029432..65065088)
    See POLA2 in Epigenomics, MapViewer

    Chromosome 11 - NC_000011.9Genomic Context describing neighboring genes Neighboring gene uncharacterized LOC728975 Neighboring gene calpain 1, (mu/I) large subunit Neighboring gene solute carrier family 22, member 20 Neighboring gene CDC42 effector protein (Rho GTPase binding) 2 Neighboring gene D4, zinc and double PHD fingers family 2

    Genomic regions, transcripts, and products

    Bibliography

    GeneRIFs: Gene References Into Functions What's a GeneRIF?

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description
    Q14181 Q9H8T0 AKTIP    HPRD  PubMed  
    Q14181 Q9H1I8 ASCC2    HPRD  PubMed  
    Q14181 P36542 ATP5C1    HPRD  PubMed  
    Q14181 Q8IW50 C9orf25    HPRD  PubMed  
    Q14181 P16870 CPE    HPRD  PubMed  
    Q14181 Q96HD1 CRELD1    HPRD  PubMed  
    Q14181 Q8IU60 DCP2    HPRD  PubMed  
    Q14181 Q9GZR7 DDX24    HPRD  PubMed  
    Q14181 Q14147 DHX34    HPRD  PubMed  
    Q14181 P56537 EIF6    HPRD  PubMed  
    Q14181 Q15910 EZH2    HPRD  PubMed  
    Q14181 Q9BTY7 FAM203A    HPRD  PubMed  
    Q14181 P22607 FGFR3    HPRD  PubMed  
    Q14181 Helicase B HELB    HPRD  PubMed  
    Q14181 P17936 IGFBP3    HPRD  PubMed  
    Q14181 Q99612 KLF6    HPRD  PubMed  
    Q14181 P30533 LRPAP1    HPRD  PubMed  
    Q14181 P55769 NHP2L1    HPRD  PubMed  
    Q14181 P09884 POLA1    HPRD  PubMed  
    Q14181 FLJ14936 PRPF38A    HPRD  PubMed  
    Q14181 Q86U06 RBM23    HPRD  PubMed  
    Q14181 Q15047 SETDB1    HPRD  PubMed  
    Q14181 Q8N2I9 STK40    HPRD  PubMed  
    Q14181 Q8IYT8 ULK2    HPRD  PubMed  
    Q14181 Q9NQW7 XPNPEP1    HPRD  PubMed  
    BioGRID:117176 BioGRID:122157 AKTIP    BioGRID  PubMed Two-hybrid 
    BioGRID:117176 BioGRID:123921 ASCC2    BioGRID  PubMed Two-hybrid 
    BioGRID:117176 BioGRID:106997 ATP5C1    BioGRID  PubMed Two-hybrid 
    BioGRID:117176 BioGRID:128461 C9orf25    BioGRID  PubMed Two-hybrid 
    BioGRID:117176 BioGRID:107755 CPE    BioGRID  PubMed Two-hybrid 
    BioGRID:117176 BioGRID:122458 CRELD1    BioGRID  PubMed Two-hybrid 
    BioGRID:117176 BioGRID:121353 DDX24    BioGRID  PubMed Two-hybrid 
    BioGRID:117176 BioGRID:115056 DHX34    BioGRID  PubMed Two-hybrid 
    BioGRID:117176 BioGRID:109898 EIF6    BioGRID  PubMed Two-hybrid 
    BioGRID:117176 BioGRID:108446 EZH2    BioGRID  PubMed Two-hybrid 
    BioGRID:117176 BioGRID:119398 FAM203A    BioGRID  PubMed Two-hybrid 
    BioGRID:117176 BioGRID:108552 FGFR3    BioGRID  PubMed Two-hybrid 
    BioGRID:117176 BioGRID:108765 FRZB    BioGRID  PubMed Two-hybrid 
    BioGRID:117176 BioGRID:115330 HDAC6    BioGRID  PubMed Two-hybrid 
    BioGRID:117176 BioGRID:124978 HELB    BioGRID  PubMed Reconstituted Complex 
    BioGRID:117176 BioGRID:109707 IGFBP3    BioGRID  PubMed Two-hybrid 
    BioGRID:117176 BioGRID:115326 KCNE3    BioGRID  PubMed Two-hybrid 
    BioGRID:117176 BioGRID:107711 KLF6    BioGRID  PubMed Two-hybrid 
    BioGRID:117176 BioGRID:110221 LRPAP1    BioGRID  PubMed Two-hybrid 
    BioGRID:117176 BioGRID:110874 NHP2L1    BioGRID  PubMed Two-hybrid 
    BioGRID:117176 BioGRID:109407 NR4A1    BioGRID  PubMed Two-hybrid 
    BioGRID:117176 BioGRID:106652 PARP1    BioGRID  PubMed Affinity Capture-Western; Far Western 
    BioGRID:117176 BioGRID:111418 POLA1    BioGRID  PubMed Reconstituted Complex 
    BioGRID:117176 BioGRID:124382 PRPF38A    BioGRID  PubMed Two-hybrid 
    BioGRID:117176 BioGRID:114054 RAE1    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:117176 BioGRID:120450 RBM23    BioGRID  PubMed Two-hybrid 
    BioGRID:117176 BioGRID:115202 SETDB1    BioGRID  PubMed Two-hybrid 
    BioGRID:117176 BioGRID:123816 STK40    BioGRID  PubMed Two-hybrid 
    BioGRID:117176 BioGRID:115058 ULK2    BioGRID  PubMed Two-hybrid 
    BioGRID:117176 BioGRID:113346 XPNPEP1    BioGRID  PubMed Two-hybrid 

    General gene information

    Markers

    Homology

    Pathways from BioSystems

    • Activation of the pre-replicative complex, organism-specific biosystem (from REACTOME)
      Activation of the pre-replicative complex, organism-specific biosystemIn S. cerevisiae, two ORC subunits, Orc1 and Orc5, both bind ATP, and Orc1 in addition has ATPase activity. Both ATP binding and ATP hydrolysis appear to be essential functions in vivo. ATP binding b...
    • Cell Cycle, organism-specific biosystem (from REACTOME)
      Cell Cycle, organism-specific biosystem
      Cell Cycle
    • Cell Cycle, Mitotic, organism-specific biosystem (from REACTOME)
      Cell Cycle, Mitotic, organism-specific biosystemThe replication of the genome and the subsequent segregation of chromosomes into daughter cells are controlled by a series of events collectively known as the cell cycle. DNA replication is carried o...
    • Chromosome Maintenance, organism-specific biosystem (from REACTOME)
      Chromosome Maintenance, organism-specific biosystemChromosome maintenance is critical for stable chromosome function in mammalian and other eukaryotic cells. Aspects of telomere maintenance and nucleosome assembly are covered here.
    • DNA Replication, organism-specific biosystem (from WikiPathways)
      DNA Replication, organism-specific biosystemStudies in the past decade have suggested that the basic mechanism of DNA replication initiation is conserved in all kingdoms of life. Initiation in unicellular eukaryotes, in particular Saccharomyce...
    • DNA Replication, organism-specific biosystem (from REACTOME)
      DNA Replication, organism-specific biosystemStudies in the past decade have suggested that the basic mechanism of DNA replication initiation is conserved in all kingdoms of life. Initiation in unicellular eukaryotes, in particular Saccharomyce...
    • DNA Replication Pre-Initiation, organism-specific biosystem (from REACTOME)
      DNA Replication Pre-Initiation, organism-specific biosystemAlthough, DNA replication occurs in the S phase of the cell cycle, the formation of the DNA replication pre-initiation complex begins during G1 phase.
    • DNA polymerase alpha / primase complex, organism-specific biosystem (from KEGG)
      DNA polymerase alpha / primase complex, organism-specific biosystemStructural complex; Genetic information processing; DNA polymerase
    • DNA replication, organism-specific biosystem (from KEGG)
      DNA replication, organism-specific biosystemA complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (D...
    • DNA replication, conserved biosystem (from KEGG)
      DNA replication, conserved biosystemA complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (D...
    • DNA replication initiation, organism-specific biosystem (from REACTOME)
      DNA replication initiation, organism-specific biosystemDNA polymerases are not capable of de novo DNA synthesis and require synthesis of a primer, usually by a DNA-dependent RNA polymerase (primase) to begin DNA synthesis. In eukaryotic cells, the primer...
    • DNA strand elongation, organism-specific biosystem (from REACTOME)
      DNA strand elongation, organism-specific biosystemAccurate and efficient genome duplication requires coordinated processes to replicate two template strands at eucaryotic replication forks. Knowledge of the fundamental reactions involved in replicat...
    • E2F mediated regulation of DNA replication, organism-specific biosystem (from REACTOME)
      E2F mediated regulation of DNA replication, organism-specific biosystemProgression through G1 and G1 to S-phase transition that initiates DNA synthesis involve many complexes that are regulated by Rb:E2F pathway. Rb:E2F pathway plays a key role in gene expression regul...
    • Extension of Telomeres, organism-specific biosystem (from REACTOME)
      Extension of Telomeres, organism-specific biosystemTelomerase acts as reverse transcriptase in the elongation of telomeres (Smogorzewska and de Lange 2004).
    • G1 to S cell cycle control, organism-specific biosystem (from WikiPathways)
      G1 to S cell cycle control, organism-specific biosystemIn the G1 phase there are two types of DNA damage responses, the p53-dependent and the p53-independent pathways. The p53-dependent responses inhibit CDKs through the up-regulation of genes encoding C...
    • G1/S Transition, organism-specific biosystem (from REACTOME)
      G1/S Transition, organism-specific biosystemCyclin E - Cdk2 complexes control the transition from G1 into S-phase. In this case, the binding of p21Cip1/Waf1 or p27kip1 is inhibitory. Important substrates for Cyclin E - Cdk2 complexes include p...
    • Inhibition of replication initiation of damaged DNA by Rb/E2F1, organism-specific biosystem (from REACTOME)
      Inhibition of replication initiation of damaged DNA by Rb/E2F1, organism-specific biosystem
      Inhibition of replication initiation of damaged DNA by Rb/E2F1
    • Lagging Strand Synthesis, organism-specific biosystem (from REACTOME)
      Lagging Strand Synthesis, organism-specific biosystemDue to the antiparallel nature of DNA, DNA polymerization is unidirectional, and one strand is synthesized discontinuously. This strand is called the lagging strand. Although the polymerase switching...
    • Leading Strand Synthesis, organism-specific biosystem (from REACTOME)
      Leading Strand Synthesis, organism-specific biosystemThe processive complex is responsible for synthesizing at least 5-10 kb of DNA in a continuous manner during leading strand synthesis. The incorporation of nucleotides by pol delta is quite accurate....
    • M/G1 Transition, organism-specific biosystem (from REACTOME)
      M/G1 Transition, organism-specific biosystemFinally, progression out of mitosis and division of the cell into two daughters (cytokinesis) requires the inactivation of Cyclin B - Cdc2 by ubiquitin-dependent proteolysis of Cyclin A and B, which ...
    • Metabolic pathways, organism-specific biosystem (from KEGG)
      Metabolic pathways, organism-specific biosystem
      Metabolic pathways
    • Mitotic G1-G1/S phases, organism-specific biosystem (from REACTOME)
      Mitotic G1-G1/S phases, organism-specific biosystem
      Mitotic G1-G1/S phases
    • Mitotic M-M/G1 phases, organism-specific biosystem (from REACTOME)
      Mitotic M-M/G1 phases, organism-specific biosystem
      Mitotic M-M/G1 phases
    • Polymerase switching, organism-specific biosystem (from REACTOME)
      Polymerase switching, organism-specific biosystemAfter the primers are synthesized, Replication Factor C binds to the 3'-end of the initiator DNA to trigger polymerase switching. The non-processive nature of pol alpha catalytic activity and the tig...
    • Polymerase switching on the C-strand of the telomere, organism-specific biosystem (from REACTOME)
      Polymerase switching on the C-strand of the telomere, organism-specific biosystemAfter the primers are synthesized on the G-Rich strand, Replication Factor C binds to the 3'-end of the initiator DNA to trigger polymerase switching. The non-processive nature of pol alpha catalytic...
    • Processive synthesis on the lagging strand, organism-specific biosystem (from REACTOME)
      Processive synthesis on the lagging strand, organism-specific biosystemThe key event that allows the processive synthesis on the lagging strand, is polymerase switching from pol alpha to pol delta, as on the leading strand. However, the processive synthesis on the laggi...
    • Purine metabolism, organism-specific biosystem (from KEGG)
      Purine metabolism, organism-specific biosystem
      Purine metabolism
    • Purine metabolism, conserved biosystem (from KEGG)
      Purine metabolism, conserved biosystem
      Purine metabolism
    • Pyrimidine metabolism, organism-specific biosystem (from KEGG)
      Pyrimidine metabolism, organism-specific biosystem
      Pyrimidine metabolism
    • Pyrimidine metabolism, conserved biosystem (from KEGG)
      Pyrimidine metabolism, conserved biosystem
      Pyrimidine metabolism
    • Removal of the Flap Intermediate, organism-specific biosystem (from REACTOME)
      Removal of the Flap Intermediate, organism-specific biosystemTwo endonucleases, Dna2 and flap endonuclease 1 (FEN-1), are responsible for resolving the nascent flap structure (Tsurimoto and Stillman 1991). The Dna2 endonuclease/helicase in yeast is a monomer o...
    • S Phase, organism-specific biosystem (from REACTOME)
      S Phase, organism-specific biosystemDNA synthesis occurs in the S phase, or the synthesis phase, of the cell cycle. The cell duplicates its hereditary material, and two copies of the chromosome are formed. As DNA replication continu...
    • Synthesis of DNA, organism-specific biosystem (from REACTOME)
      Synthesis of DNA, organism-specific biosystemThe actual synthesis of DNA occurs in the S phase of the cell cycle. This includes the initiation of DNA replication, when the first nucleotide of the new strand is laid down during the synthesis of...
    • Telomere C-strand (Lagging Strand) Synthesis, organism-specific biosystem (from REACTOME)
      Telomere C-strand (Lagging Strand) Synthesis, organism-specific biosystemDue to the antiparallel nature of DNA, DNA polymerization is unidirectional, and one strand is synthesized discontinuously. This strand is called the lagging strand. Although the polymerase switching...
    • Telomere C-strand synthesis initiation, organism-specific biosystem (from REACTOME)
      Telomere C-strand synthesis initiation, organism-specific biosystemDNA polymerases are not capable of de novo DNA synthesis and require synthesis of a primer, usually by a DNA-dependent RNA polymerase (primase) to begin DNA synthesis. In eukaryotic cells, the primer...
    • Telomere Maintenance, organism-specific biosystem (from REACTOME)
      Telomere Maintenance, organism-specific biosystemTelomeres are protein-DNA complexes at the ends of linear chromosomes that are important for genome stability. Telomeric DNA in humans, as in many eukaryotic organisms, consists of tandem repeats (B...

    Clone Names

    • FLJ21662, FLJ37250

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    DNA binding IEA
    Inferred from Electronic Annotation
    more info
     
    DNA-directed DNA polymerase activity IEA
    Inferred from Electronic Annotation
    more info
     
    molecular_function ND
    No biological Data available
    more info
     
    protein heterodimerization activity IEA
    Inferred from Electronic Annotation
    more info
     
    Process Evidence Code Pubs
    DNA replication NAS
    Non-traceable Author Statement
    more info
    PubMed 
    DNA strand elongation involved in DNA replication TAS
    Traceable Author Statement
    more info
     
    DNA-dependent DNA replication initiation TAS
    Traceable Author Statement
    more info
     
    G1/S transition of mitotic cell cycle TAS
    Traceable Author Statement
    more info
     
    M/G1 transition of mitotic cell cycle TAS
    Traceable Author Statement
    more info
     
    S phase of mitotic cell cycle TAS
    Traceable Author Statement
    more info
     
    mitotic cell cycle TAS
    Traceable Author Statement
    more info
     
    protein import into nucleus, translocation IEA
    Inferred from Electronic Annotation
    more info
     
    telomere maintenance TAS
    Traceable Author Statement
    more info
     
    telomere maintenance via recombination TAS
    Traceable Author Statement
    more info
     
    telomere maintenance via semi-conservative replication TAS
    Traceable Author Statement
    more info
     
    Component Evidence Code Pubs
    alpha DNA polymerase:primase complex IEA
    Inferred from Electronic Annotation
    more info
     
    cytoplasm IDA
    Inferred from Direct Assay
    more info
    PubMed 
    NOT nucleolus IDA
    Inferred from Direct Assay
    more info
    PubMed 
    nucleoplasm TAS
    Traceable Author Statement
    more info
     
    nucleus IDA
    Inferred from Direct Assay
    more info
    PubMed 
    nucleus NAS
    Non-traceable Author Statement
    more info
     

    General protein information

    Preferred Names
    DNA polymerase alpha subunit B
    Names
    DNA polymerase alpha subunit B
    DNA polymerase alpha 70 kDa subunit
    polymerase (DNA-directed), alpha (70kD)

    NCBI Reference Sequences (RefSeq)

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_002689.2NP_002680.2  DNA polymerase alpha subunit B

      Status: PROVISIONAL

      Source sequence(s)
      BC002990
      Consensus CDS
      CCDS8098.1
      UniProtKB/Swiss-Prot
      Q14181
      Related
      ENSP00000265465, OTTHUMP00000231593, ENST00000265465, OTTHUMT00000387223
      Conserved Domains (2) summary
      pfam08418
      Location:15248
      Blast Score: 379
      Pol_alpha_B_N; DNA polymerase alpha subunit B N-terminal
      cl13995
      Location:358549
      Blast Score: 422
      MPP_superfamily; metallophosphatase superfamily, metallophosphatase domain

    RefSeqs of Annotated Genomes: Build 37.3

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh37.p5 Primary Assembly

    Genomic

    1. NC_000011.9 Reference GRCh37.p5 Primary Assembly

      Range
      65029432..65065088
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate HuRef

    Genomic

    1. AC_000143.1 Alternate HuRef

      Range
      61356467..61392125
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Related Sequences

    Nucleotide Protein
    Heading Accession and Version
    genomic AP000944.4 (2810..38463) None
    genomic CH471076.1 EAW74373.1
    mRNA AK025315.1 None
    mRNA AK094569.1 BAG52888.1
    mRNA AK297842.1 BAG60176.1
    mRNA AK307831.1 None
    mRNA AK309284.1 None
    mRNA BC001347.2 AAH01347.1
    mRNA BC002990.1 AAH02990.1
    mRNA BC018813.2 None
    mRNA BC018814.2 None
    mRNA CR456737.1 CAG33018.1
    mRNA L24559.1 AAA16459.1
    other-genetic DQ892023.2 ABM82949.1
    other-genetic DQ895215.2 ABM86141.1
    Protein Accession Links
    GenePept Link UniProtKB Link
    Q14181.2 GenPept UniProtKB/Swiss-Prot:Q14181

      Supplemental Content

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