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    FHL3 four and a half LIM domains 3 [ Homo sapiens ]

    Gene ID: 2275, updated on 11-May-2012
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    Summary

    Official Symbol
    FHL3provided by HGNC
    Official Full Name
    four and a half LIM domains 3provided by HGNC
    Primary source
    HGNC:3704
    See related
    Ensembl:ENSG00000183386; HPRD:09101; MIM:602790; Vega:OTTHUMG00000004434
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    SLIM2; MGC8696; MGC19547; MGC23614
    Summary
    The protein encoded by this gene is a member of a family of proteins containing a four-and-a-half LIM domain, which is a highly conserved double zinc finger motif. The encoded protein has been shown to interact with the cancer developmental regulators SMAD2, SMAD3, and SMAD4, the skeletal muscle myogenesis protein MyoD, and the high-affinity IgE beta chain regulator MZF-1. This protein may be involved in tumor suppression, repression of MyoD expression, and repression of IgE receptor expression. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
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    Genomic context

    Location :
    1p34
    Sequence :
    Chromosome: 1; NC_000001.10 (38462442..38471234, complement)
    See FHL3 in Epigenomics, MapViewer

    Chromosome 1 - NC_000001.10Genomic Context describing neighboring genes Neighboring gene inositol polyphosphate-5-phosphatase, 75kDa Neighboring gene splicing factor 3a, subunit 3, 60kDa Neighboring gene UTP11-like, U3 small nucleolar ribonucleoprotein, (yeast) Neighboring gene POU class 3 homeobox 1

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    Genomic regions, transcripts, and products

    Go to nucleotideGraphics FASTA GenBank

    Go to reference sequence details
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    Bibliography

    Related articles in PubMed

    1. Four-and-a-half LIM domain proteins inhibit transactivation by hypoxia-inducible factor 1. Hubbi ME, et al. J Biol Chem, 2012 Feb 24. PMID 22219185.
    2. A cohesin-RAD21 interactome. Panigrahi AK, et al. Biochem J, 2012 Mar 15. PMID 22145905.
    3. A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles. Wagner SA, et al. Mol Cell Proteomics, 2011 Oct. PMID 21890473.
    4. Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level. Danielsen JM, et al. Mol Cell Proteomics, 2011 Mar. PMID 21139048.
    5. A human MAP kinase interactome. Bandyopadhyay S, et al. Nat Methods, 2010 Oct. PMID 20936779.

    See all (24) citations in PubMed

    See citations in PubMed for homologs of this gene provided by HomoloGene

    GeneRIFs: Gene References Into Functions What's a GeneRIF?

    1. FHL3 does not bind to HIF-1alpha or p300, indicating that it regulates transactivation by a novel molecular mechanism.
      Title: Four-and-a-half LIM domain proteins inhibit transactivation by hypoxia-inducible factor 1.
    2. the second zinc finger motif in LIM4 was responsible for the auto-activation of FHL3.
      Title: Identification of the transactivation domain of the human FHL3.
    3. Human four-and-a-half LIM family members suppress tumor cell growth through a TGF-beta-like signaling pathway.
      Title: Human four-and-a-half LIM family members suppress tumor cell growth through a TGF-beta-like signaling pathway.
    4. FHL2 and FHL3, respectively, are colocalized with alpha(7)beta(1) integrin receptor at the periphery of Z-discs, suggesting a role in mechanical stabilization of muscle cells
      Title: The LIM-only proteins FHL2 and FHL3 interact with alpha- and beta-subunits of the muscle alpha7beta1 integrin receptor.
    5. MZF-1 and FHL3 formed a complex of high molecular mass with some additional proteins in the nucleus. Transcriptional repression of FcepsilonRI by MZF-1 required FHL3.
      Title: FHL3 negatively regulates human high-affinity IgE receptor beta-chain gene expression by acting as a transcriptional co-repressor of MZF-1.
    6. FHL3 association with MyoD may contribute to the regulation of MyoD-dependent transcription of muscle genes and thereby myogenesis
      Title: FHL3 binds MyoD and negatively regulates myotube formation.
    7. Data show that FHL3 (human four-and-a-half LIM-only protein 3) interacts with human phosphatase CDC25B in the cell nucleus.
      Title: LIM-only protein FHL3 interacts with CDC25B2 phosphatase.
    Submit: New GeneRIF Correction
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    Phenotypes

    Review eQTL and phenotype association data in this region using PheGenI

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    Interactions

    Products Interactant Other Gene Complex Source Pubs Description
    NP_004459.2 NP_003122.1 SRF    BIND  PubMed SRF interacts with FHL3. This interaction was modeled on a demonstrated interaction between human SRF and FHL3 from an unspecified species. 
    Q13643 P60709 ACTB    HPRD  PubMed  
    Q13643 Q6UY14 ADAMTSL4    HPRD  PubMed  
    Q13643 Q13155 AIMP2    HPRD  PubMed  
    Q13643 P30305 CDC25B    HPRD  PubMed  
    Q13643 Q00587 CDC42EP1    HPRD  PubMed  
    Q13643 P56545 CTBP2    HPRD  PubMed  
    Q13643 Q14192 FHL2    HPRD  PubMed  
    Q13643 Q13643 FHL3    HPRD  PubMed  
    Q13643 Q13683 ITGA7    HPRD  PubMed  
    Q13643 O95600 KLF8    HPRD  PubMed  
    Q13643 Q14847 LASP1    HPRD  PubMed  
    Q13643 Q8TBB1 LNX1    HPRD  PubMed  
    Q13643 P28482 MAPK1    HPRD  PubMed  
    Q13643 Q96RN5 MED15    HPRD  PubMed  
    Q13643 P28698 MZF1    HPRD  PubMed  
    Q13643 Q9P286 PAK7    HPRD  PubMed  
    Q13643 Q8IXK0 PHC2    HPRD  PubMed  
    Q13643 RNA binding motif protein 42 RBM42    HPRD  PubMed  
    Q13643 Regulatory factor X domain containing 1 RFX6    HPRD  PubMed  
    Q13643 P11831 SRF    HPRD  PubMed  
    Q13643 Q15654 TRIP6    HPRD  PubMed  
    BioGRID:108566 BioGRID:120002 ADAMTSL4    BioGRID  PubMed Two-hybrid 
    BioGRID:108566 BioGRID:113684 AIMP2    BioGRID  PubMed Two-hybrid 
    BioGRID:108566 BioGRID:116308 CDC42EP1    BioGRID  PubMed Two-hybrid 
    BioGRID:108566 BioGRID:107775 CREB1    BioGRID  PubMed Affinity Capture-Western; Two-hybrid 
    BioGRID:108566 BioGRID:107870 CTBP2    BioGRID  PubMed Reconstituted Complex; Two-hybrid 
    BioGRID:108566 BioGRID:108565 FHL2    BioGRID  PubMed FRET; Two-hybrid 
    BioGRID:108566 BioGRID:108566 FHL3    BioGRID  PubMed Two-hybrid 
    BioGRID:108566 BioGRID:114846 FHL5    BioGRID  PubMed Two-hybrid 
    BioGRID:108566 BioGRID:109885 ITGA7    BioGRID  PubMed Affinity Capture-Western; Co-localization; Two-hybrid 
    BioGRID:108566 BioGRID:119426 KLF3    BioGRID  PubMed Affinity Capture-Western; Reconstituted Complex; Two-hybrid 
    BioGRID:108566 BioGRID:110120 LASP1    BioGRID  PubMed Two-hybrid 
    BioGRID:108566 BioGRID:124219 LNX1    BioGRID  PubMed Two-hybrid 
    BioGRID:108566 BioGRID:119623 MED15    BioGRID  PubMed Two-hybrid 
    BioGRID:108566 BioGRID:121402 PAK7    BioGRID  PubMed Two-hybrid 
    BioGRID:108566 BioGRID:108234 PHC2    BioGRID  PubMed Two-hybrid 
    BioGRID:108566 BioGRID:111822 RAD21    BioGRID  PubMed Affinity Capture-Western; Two-hybrid 
    BioGRID:108566 BioGRID:122588 RBM42    BioGRID  PubMed Two-hybrid 
    BioGRID:108566 BioGRID:128803 RFX6    BioGRID  PubMed Two-hybrid 
    BioGRID:108566 BioGRID:112599 SREBF2    BioGRID  PubMed Two-hybrid 
    BioGRID:108566 BioGRID:113056 TRIP6    BioGRID  PubMed Two-hybrid 
    BioGRID:108566 BioGRID:113164 UBC    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:108566 BioGRID:113498 ZBTB16    BioGRID  PubMed Phenotypic Enhancement 
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    General gene information

    Markers

    Genotypes

    Homology

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    actin binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    actin cytoskeleton organization IEA
    Inferred from Electronic Annotation
    more info
    		  
    muscle organ development TAS
    Traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    Z disc IEA
    Inferred from Electronic Annotation
    more info
    		  
    nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    stress fiber IEA
    Inferred from Electronic Annotation
    more info
    		  
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    General protein information

    Preferred Names
    four and a half LIM domains protein 3
    Names
    four and a half LIM domains protein 3
    FHL-3
    SLIM-2
    LIM-only protein FHL3
    skeletal muscle LIM-protein 2
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    NCBI Reference Sequences (RefSeq)

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_001243878.1 → NP_001230807.1  four and a half LIM domains protein 3 isoform 2

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1.
      Source sequence(s)
      AK290641, BC001351, BC014501
      UniProtKB/Swiss-Prot
      Q13643
      UniProtKB/TrEMBL
      Q96C98
      Conserved Domains (3) summary
      cd09346
      Location:54 – 105
      Blast Score: 228
      LIM3_FHL; The third LIM domain of Four and a half LIM domains protein (FHL)
      cd09434
      Location:113 – 168
      Blast Score: 237
      LIM4_FHL3; The fourth LIM domain of Four and a half LIM domains protein 3 (FHL3)
      cl02475
      Location:1 – 47
      Blast Score: 223
      LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers.

    2. NM_004468.4 → NP_004459.2  four and a half LIM domains protein 3 isoform 1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
      Source sequence(s)
      AK290641, BC001351
      Consensus CDS
      CCDS30678.1
      UniProtKB/Swiss-Prot
      Q13643
      Related
      ENSP00000362107, OTTHUMP00000004724, ENST00000373016, OTTHUMT00000012958
      Conserved Domains (5) summary
      cd09346
      Location:162 – 213
      Blast Score: 221
      LIM3_FHL; The third LIM domain of Four and a half LIM domains protein (FHL)
      cd09423
      Location:36 – 94
      Blast Score: 250
      LIM1_FHL3; The first LIM domain of Four and a half LIM domains protein 3 (FHL3)
      cd09427
      Location:98 – 155
      Blast Score: 255
      LIM2_FHL3; The second LIM domain of Four and a half LIM domains protein 3 (FHL3)
      cd09434
      Location:221 – 276
      Blast Score: 234
      LIM4_FHL3; The fourth LIM domain of Four and a half LIM domains protein 3 (FHL3)
      cl02475
      Location:2 – 33
      Blast Score: 81
      LIM; LIM is a small protein-protein interaction domain, containing two zinc fingers.

    RefSeqs of Annotated Genomes: Build 37.3

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh37.p5 Primary Assembly

    Genomic

    1. NC_000001.10 Reference GRCh37.p5 Primary Assembly

      Range
      38462442..38471234, complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate HuRef

    Genomic

    1. AC_000133.1 Alternate HuRef

      Range
      36580786..36583450, complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
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    Related Sequences

    Nucleotide Protein
    Heading Accession and Version
    genomic AL603790.41 CAH69931.1
    genomic CH471059.2 EAX07301.1
      EAX07302.1
    mRNA AF133732.1 AAF61376.1
    mRNA AK290641.1 BAF83330.1
    mRNA AK309179.1 None
    mRNA BC001351.2 AAH01351.1
    mRNA BC011697.2 AAH11697.1
    mRNA BC014501.1 AAH14501.1
    mRNA BT007052.1 AAP35701.1
    mRNA BX384389.2 None
    mRNA CR457425.1 CAG33706.1
    mRNA U60116.2 AAC04466.2
    other-genetic DQ890595.2 ABM92212.1
    other-genetic DQ893763.2 ABM84689.1
    other-genetic DQ895996.2 ABM86964.1
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q13643.4 GenPept UniProtKB/Swiss-Prot:Q13643
    Q96C98 GenPept UniProtKB/TrEMBL:Q96C98
    Q9P100 GenPept UniProtKB/TrEMBL:Q9P100
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    Additional Links

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

    Chemical Information
    Molecular Biology Databases
    Research Materials
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