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    FH fumarate hydratase [ Homo sapiens ]

    Gene ID: 2271, updated on 12-May-2012

    Summary

    Official Symbol
    FHprovided by HGNC
    Official Full Name
    fumarate hydrataseprovided by HGNC
    Primary source
    HGNC:3700
    See related
    Ensembl:ENSG00000091483; HPRD:00652; MIM:136850; Vega:OTTHUMG00000039597
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    MCL; LRCC; HLRCC; MCUL1
    Summary
    The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq, Jul 2008]

    Genomic context

    Location :
    1q42.1
    Sequence :
    Chromosome: 1; NC_000001.10 (241660857..241683085, complement)

    Chromosome 1 - NC_000001.10Genomic Context describing neighboring genes Neighboring gene regulator of G-protein signaling 7 Neighboring gene microRNA 3123 Neighboring gene ribosomal protein L36 pseudogene 6 Neighboring gene kynurenine 3-monooxygenase (kynurenine 3-hydroxylase) Neighboring gene opsin 3 Neighboring gene choroideremia-like (Rab escort protein 2)

    Genomic regions, transcripts, and products

    Bibliography

    GeneRIFs: Gene References Into Functions What's a GeneRIF?

    Phenotypes

    Fumarase deficiency

    Summary from GeneReviews: Go to GeneReviews

    Disease Characteristics
    Fumarate hydratase deficiency is characterized by progressive neurologic abnormalities that first manifest prenatally as polyhydramnios and brain malformations (periventricular cysts, Dandy-Walker malformation, agenesis of the corpus callosum, deficient closure of the sylvian opercula, diffuse bilateral polymicrogyria, and enlarged cerebral ventricles). Neonates demonstrate severe neurologic abnormalities, poor feeding, failure to thrive, and hypotonia. Infants can have seizures, infantile spasms, severe developmental delay, and microcephaly along with limb dystonia, athetosis, and autistic features. Neonatal polycythemia, leukopenia, neutropenia, and mild hepatosplenomegaly can be seen. Neuroimaging may reveal mild hypomyelination, progressive cerebral atrophy, and ventricular dilatation. Many children with fumarate hydratase deficiency do not survive infancy or childhood; those surviving beyond childhood have severe psychomotor retardation.
    Diagnosis Testing
    Isolated increased concentration of fumaric acid on urine organic acid analysis is highly suggestive of fumarate hydratase deficiency. The diagnosis is confirmed by identification of deficient fumarate hydratase enzyme activity in fibroblasts, lymphoblasts, or white blood cells and/or by molecular genetic testing of FH, the gene that encodes fumarate hydratase and the only gene known to be associated with fumarate hydratase deficiency. Fumarate hydratase enzyme activity in severely affected individuals is generally less than 10% of the control mean; however, residual fumarate hydratase enzyme activity in some affected individuals can be 11%-35% of the control mean, overlapping with that seen in some obligate heterozygotes.
    Genetic Counseling
    Fumarate hydratase deficiency is inherited in an autosomal recessive manner. When both parents are known to be heterozygotes (i.e., carriers of an FH mutation), each sib of an affected individual has at conception a 25% chance of having fumarate hydratase deficiency and a 25% chance of having no mutation in the FH gene. Each sib also has a 50% chance of being a heterozygote. Heterozygotes have a higher than average risk of developing cutaneous leiomyomas and in females, uterine leiomyomas or fibroids; however, the absolute risk is unknown. Carrier testing for at-risk family members is possible once the FH mutations have been identified in the family. Prenatal diagnosis for pregnancies at increased risk for fumarate hydratase deficiency is possible either by measurement of fumarate hydratase enzyme activity or by molecular genetic testing if both disease-causing mutations in the family are known.
    References

    Leiomyomatosis and renal cell cancer

    Summary from GeneReviews: Go to GeneReviews

    Disease Characteristics
    Hereditary leiomyomatosis and renal cell cancer (HLRCC) is characterized by cutaneous leiomyomata (multiple or single in 76% of affected individuals), uterine leiomyomata (fibroids), and/or a single renal tumor. Cutaneous leiomyomata appear as skin-colored to light brown papules or nodules distributed over the trunk and extremities, and occasionally on the face, and appear at a mean age of 25 years, increasing in size and number with age. Uterine leiomyomata are present in almost all females with HLRCC and tend to be numerous and large; age at diagnosis ranges from 18 to 52 years, with most women experiencing irregular or heavy menstruation and pelvic pain. Renal tumors causing hematuria, lower back pain, and a palpable mass are usually unilateral, solitary, and aggressive and range from type 2 papillary to tubulo-papillary to collecting-duct carcinomas. They occur in about 10%-16% of individuals with HLRCC; the median age of detection is 44 years.
    Diagnosis Testing
    HLRCC is diagnosed by the presence of multiple cutaneous leiomyomas, with at least one histologically confirmed leiomyoma, or by a single leiomyoma in the presence of a positive family history of HLRCC. Diagnosis is confirmed by testing of fumarate hydratase enzyme activity in cultured skin fibroblasts or lymphoblastoid cells showing reduced activity ( 60%) or by molecular genetic testing. Molecular genetic testing of FH, the only gene known to be associated with HLRCC, is available on a clinical basis.
    Genetic Counseling
    HLRCC is inherited in an autosomal dominant manner. If a parent of a proband is clinically affected or has a disease-causing mutation, the sibs of the proband have a 50% chance of inheriting the mutation. Each child of an individual with HLRCC has a 50% chance of inheriting the mutation. The degree of clinical severity is not predictable. Prenatal testing for pregnancies at increased risk is possible if the disease-causing mutation in a family is known.
    References

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description
    P07954 P40926 MDH2    HPRD  PubMed  
    BioGRID:108562 BioGRID:109142 GRB2    BioGRID  PubMed Two-hybrid 
    BioGRID:108562 BioGRID:110356 MDH2    BioGRID  PubMed Reconstituted Complex 
    BioGRID:108562 BioGRID:113164 UBC    BioGRID  PubMed Affinity Capture-MS 

    General gene information

    Markers

    Homology

    • Homologs of the FH gene: The FH gene is conserved in chimpanzee, , dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, S.cerevisiae, K.lactis, , S.pombe, , N.crassa, and A.thaliana.
    • Map Viewer (Mouse, Rat)

    Pathways from BioSystems

    • Citrate cycle (TCA cycle), organism-specific biosystem (from KEGG)
      Citrate cycle (TCA cycle), organism-specific biosystemThe citrate cycle (TCA cycle, Krebs cycle) is an important aerobic pathway for the final steps of the oxidation of carbohydrates and fatty acids. The cycle starts with acetyl-CoA, the activated form ...
    • Citrate cycle (TCA cycle), conserved biosystem (from KEGG)
      Citrate cycle (TCA cycle), conserved biosystemThe citrate cycle (TCA cycle, Krebs cycle) is an important aerobic pathway for the final steps of the oxidation of carbohydrates and fatty acids. The cycle starts with acetyl-CoA, the activated form ...
    • Citrate cycle, second carbon oxidation, 2-oxoglutarate => oxaloacetate, organism-specific biosystem (from KEGG)
      Citrate cycle, second carbon oxidation, 2-oxoglutarate => oxaloacetate, organism-specific biosystemPathway module; Carbohydrate and lipid metabolism; Central carbohydrate metabolism
    • Citrate cycle, second carbon oxidation, 2-oxoglutarate => oxaloacetate, conserved biosystem (from KEGG)
      Citrate cycle, second carbon oxidation, 2-oxoglutarate => oxaloacetate, conserved biosystemPathway module; Carbohydrate and lipid metabolism; Central carbohydrate metabolism
    • Citric acid cycle (TCA cycle), organism-specific biosystem (from REACTOME)
      Citric acid cycle (TCA cycle), organism-specific biosystemIn the citric acid or tricarboxylic acid (TCA) cycle, the acetyl group of acetyl CoA (derived primarily from oxidative decarboxylation of pyruvate, beta-oxidation of long-chain fatty acids, and catab...
    • Metabolic pathways, organism-specific biosystem (from KEGG)
      Metabolic pathways, organism-specific biosystem
      Metabolic pathways
    • Metabolism, organism-specific biosystem (from REACTOME)
      Metabolism, organism-specific biosystemMetabolic processes in human cells generate energy through the oxidation of molecules consumed in the diet and mediate the synthesis of diverse essential molecules not taken in the diet as well as th...
    • Pathways in cancer, organism-specific biosystem (from KEGG)
      Pathways in cancer, organism-specific biosystem
      Pathways in cancer
    • Pyruvate metabolism and Citric Acid (TCA) cycle, organism-specific biosystem (from REACTOME)
      Pyruvate metabolism and Citric Acid (TCA) cycle, organism-specific biosystemPyruvate metabolism and the citric acid (TCA) cycle together link the processes of energy metabolism in a human cell with one another and with key biosynthetic reactions. Pyruvate, derived from the r...
    • Renal cell carcinoma, organism-specific biosystem (from KEGG)
      Renal cell carcinoma, organism-specific biosystemRenal cell cancer (RCC) accounts for ~3% of human malignancies and its incidence appears to be rising. Although most cases of RCC seem to occur sporadically, an inherited predisposition to renal canc...
    • Renal cell carcinoma, conserved biosystem (from KEGG)
      Renal cell carcinoma, conserved biosystemRenal cell cancer (RCC) accounts for ~3% of human malignancies and its incidence appears to be rising. Although most cases of RCC seem to occur sporadically, an inherited predisposition to renal canc...
    • TCA Cycle, organism-specific biosystem (from WikiPathways)
      TCA Cycle, organism-specific biosystemThe [[wikipedia:citric_acid_cycle|citric acid cycle]], also known as the tricarboxylic acid cycle (TCA cycle) or the Krebs cycle, (or rarely, the Szent-Gyorgyi-Krebs cycle) is a series of enzyme-cata...
    • TCA cycle II (eukaryotic), organism-specific biosystem (from BIOCYC)
      TCA cycle II (eukaryotic), organism-specific biosystemGeneral Background The TCA pathway is a catabolic pathway of aerobic respiration. It generates energy and reducing power. It is the first step in generating precursors for biosynthesis. When ac...
    • TCA cycle II (eukaryotic), conserved biosystem (from BIOCYC)
      TCA cycle II (eukaryotic), conserved biosystemGeneral Background The TCA pathway is a catabolic pathway of aerobic respiration that generates both energy and reducing power. In addition, it is also the first step in generating precursors for bi...
    • The citric acid (TCA) cycle and respiratory electron transport, organism-specific biosystem (from REACTOME)
      The citric acid (TCA) cycle and respiratory electron transport, organism-specific biosystemThe metabolism of pyruvate provides one source of acetyl-CoA which enters the citric acid (TCA, tricarboxylic acid) cycle to generate energy and the reducing equivalent NADH. These reducing equivalen...

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    fumarate hydratase activity EXP
    Inferred from Experiment
    more info
     
    lyase activity IEA
    Inferred from Electronic Annotation
    more info
     
    Process Evidence Code Pubs
    fumarate metabolic process IEA
    Inferred from Electronic Annotation
    more info
     
    homeostasis of number of cells within a tissue IEA
    Inferred from Electronic Annotation
    more info
     
    malate metabolic process IEA
    Inferred from Electronic Annotation
    more info
     
    small molecule metabolic process TAS
    Traceable Author Statement
    more info
     
    tricarboxylic acid cycle TAS
    Traceable Author Statement
    more info
     
    Component Evidence Code Pubs
    cytoplasm IEA
    Inferred from Electronic Annotation
    more info
     
    mitochondrial matrix TAS
    Traceable Author Statement
    more info
     
    mitochondrion IEA
    Inferred from Electronic Annotation
    more info
     
    tricarboxylic acid cycle enzyme complex IEA
    Inferred from Electronic Annotation
    more info
     

    General protein information

    Preferred Names
    fumarate hydratase, mitochondrial
    Names
    fumarate hydratase, mitochondrial
    fumarase
    NP_000134.2

    NCBI Reference Sequences (RefSeq)

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    Genomic

    1. NG_012338.1 RefSeqGene

      Range
      4970..27198
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_000143.3NP_000134.2  fumarate hydratase, mitochondrial

      Status: REVIEWED

      Source sequence(s)
      AA669797, AW611924, BC003108, DA129148
      Consensus CDS
      CCDS1617.1
      UniProtKB/TrEMBL
      B1ANK7
      UniProtKB/Swiss-Prot
      P07954
      Related
      ENSP00000355518, OTTHUMP00000037573, ENST00000366560, OTTHUMT00000095490
      Conserved Domains (2) summary
      cd01362
      Location:51506
      Blast Score: 2311
      Fumarase_classII; Class II fumarases
      PRK00485
      Location:49510
      Blast Score: 2334
      fumC; fumarate hydratase; Reviewed

    RefSeqs of Annotated Genomes: Build 37.3

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh37.p5 Primary Assembly

    Genomic

    1. NC_000001.10 Reference GRCh37.p5 Primary Assembly

      Range
      241660857..241683085, complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate HuRef

    Genomic

    1. AC_000133.1 Alternate HuRef

      Range
      212119119..212141338, complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Related Sequences

    Nucleotide Protein
    Heading Accession and Version
    genomic AL359764.18 CAI15144.1
    genomic AL591898.1 CAI13908.1
    genomic AL606476.3 CAI14951.1
    genomic CH471098.1 EAW70090.1
      EAW70091.1
      EAW70092.1
      EAW70093.1
    mRNA AA669797.1 None
    mRNA AK312415.1 BAG35325.1
    mRNA AW611924.1 None
    mRNA BC003108.1 AAH03108.1
    mRNA BC017444.1 AAH17444.1
    mRNA BT009839.1 AAP88841.1
    mRNA DA129148.1 None
    mRNA M15502.1 AAA52483.1
    mRNA U48857.1 AAD00071.1
    mRNA U59309.1 AAB66354.1
    other-genetic EU176358.1 ABW03809.1
    Protein Accession Links
    GenPept Link UniProtKB Link
    P07954.3 GenPept UniProtKB/Swiss-Prot:P07954

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