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    FECH ferrochelatase [ Homo sapiens (human) ]

    Gene ID: 2235, updated on 22-May-2013
    Official Symbol
    FECHprovided by HGNC
    Official Full Name
    ferrochelataseprovided by HGNC
    Primary source
    HGNC:3647
    See related
    HPRD:01509; MIM:612386
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    EPP; FCE
    Summary
    The protein encoded by this gene is localized to the mitochondrion, where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Mutations in this gene are associated with erythropoietic protoporphyria. Two transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene is found on chromosome 3.[provided by RefSeq, May 2010]
    Location :
    18q21.3
    Sequence :
    Chromosome: 18; NC_000018.9 (55212073..55253969, complement)
    See FECH in Epigenomics, MapViewer

    Chromosome 18 - NC_000018.9Genomic Context describing neighboring genes Neighboring gene ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 3 Neighboring gene one cut homeobox 2 Neighboring gene uncharacterized LOC100505549 Neighboring gene asparaginyl-tRNA synthetase Neighboring gene ATPase, aminophospholipid transporter, class I, type 8B, member 1

    GeneRIFs: Gene References Into Functions What's a GeneRIF?

    Erythropoietic protoporphyria

    Summary from GeneReviews: Erythropoietic Protoporphyria, Autosomal Recessive Go to GeneReviews

    Disease Characteristics
    Erythropoietic protoporphyria (EPP) is characterized by cutaneous photosensitivity (usually beginning in infancy or childhood) that results in tingling, burning, pain, and itching within minutes of sun/light exposure and may be accompanied by swelling and redness. Vesicular lesions are uncommon. Symptoms, which may seem out of proportion to the visible skin lesions, may persist for hours or days after the initial phototoxic reaction. Photosensitivity usually remains for life. Multiple episodes of acute photosensitivity may lead to chronic changes of sun-exposed skin (lichenification, leathery pseudovesicles, grooving around the lips) and loss of lunulae of the nails. Approximately 20%-30% of individuals with EPP have some degree of liver dysfunction, which is typically mild with slight elevations of the liver enzymes. Up to 5% may develop more advanced liver disease which may be accompanied by motor neuropathy, similar to that seen in the acute porphyrias. Except for the small minority with advanced liver disease, life expectancy is not reduced.
    Diagnosis Testing
    Detection of markedly increased free erythrocyte protoporphyrin is the most sensitive and specific biochemical diagnostic test for EPP. Identification of biallelic mutations in FECH, encoding ferrochelatase, confirms the diagnosis.
    Genetic Counseling
    EPP is inherited in an autosomal recessive manner. In 90%-95% of cases an affected individual inherits a loss-of-function FECH allele from one parent and a low-expression FECH allele from the other parent. In about 5%-10% of cases, an affected individual has two loss-of-function FECH alleles. At conception, each sib of an affected individual has a 25% chance of being affected, a 50% chance of being an asymptomatic carrier, and a 25% chance of being unaffected and not a carrier. Heterozygotes (carriers) and individuals who inherit two low-expression alleles are asymptomatic. Carrier testing for at-risk family members and prenatal testing for pregnancies at increased risk are possible if the disease-causing mutations in the family have been identified.
    References
    Protein Gene Interaction Pubs
    pol gag-pol HIV-1 Pol is identified to have a physical interaction with ferrochelatase (FECH) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses PubMed

    Go to the HIV-1, Human Protein Interaction Database

    Products Interactant Other Gene Complex Source Pubs Description
    P22830 O75027 ABCB7    HPRD  PubMed  
    P22830 P22830 FECH    HPRD  PubMed  
    BioGRID:108526 BioGRID:106540 ABCB7    BioGRID  PubMed Affinity Capture-Western; Reconstituted Complex 
    BioGRID:108526 BioGRID:116183 COPS5    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:108526 BioGRID:116176 COPS6    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:108526 BioGRID:114030 CUL3    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:108526 BioGRID:108309 ELAVL1    BioGRID  PubMed Affinity Capture-RNA 
    BioGRID:108526 BioGRID:108526 FECH    BioGRID  PubMed Co-crystal Structure 
    BioGRID:108526 BioGRID:136699 MINOS1    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:108526 BioGRID:110455 MME    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:108526 BioGRID:206176 Ppp2r1a    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:108526 BioGRID:113164 UBC    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:108526 BioGRID:116077 USP20    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:108526 BioGRID:123904 USP42    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:108526 BioGRID:1205538 gag-pol    BioGRID  PubMed Affinity Capture-MS 

    Markers

    Homology

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    2 iron, 2 sulfur cluster binding IEA
    Inferred from Electronic Annotation
    more info
     
    ferrochelatase activity IDA
    Inferred from Direct Assay
    more info
    PubMed 
    ferrochelatase activity TAS
    Traceable Author Statement
    more info
     
    ferrous iron binding TAS
    Traceable Author Statement
    more info
    PubMed 
    heme binding IEA
    Inferred from Electronic Annotation
    more info
     
    iron-responsive element binding IEA
    Inferred from Electronic Annotation
    more info
     
    protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    Process Evidence Code Pubs
    cholesterol metabolic process IEA
    Inferred from Electronic Annotation
    more info
     
    detection of UV IEA
    Inferred from Electronic Annotation
    more info
     
    erythrocyte differentiation IEA
    Inferred from Electronic Annotation
    more info
     
    generation of precursor metabolites and energy TAS
    Traceable Author Statement
    more info
    PubMed 
    heme biosynthetic process TAS
    Traceable Author Statement
    more info
     
    iron ion homeostasis IEA
    Inferred from Electronic Annotation
    more info
     
    porphyrin-containing compound metabolic process TAS
    Traceable Author Statement
    more info
     
    protoporphyrinogen IX metabolic process IDA
    Inferred from Direct Assay
    more info
    PubMed 
    regulation of eIF2 alpha phosphorylation by heme IEA
    Inferred from Electronic Annotation
    more info
     
    regulation of hemoglobin biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
     
    response to light stimulus TAS
    Traceable Author Statement
    more info
    PubMed 
    small molecule metabolic process TAS
    Traceable Author Statement
    more info
     
    very-low-density lipoprotein particle assembly IEA
    Inferred from Electronic Annotation
    more info
     
    Component Evidence Code Pubs
    mitochondrial inner membrane IEA
    Inferred from Electronic Annotation
    more info
     
    mitochondrial matrix TAS
    Traceable Author Statement
    more info
     
    Preferred Names
    ferrochelatase, mitochondrial
    Names
    ferrochelatase, mitochondrial
    heme synthase
    protoporphyria
    heme synthetase
    protoheme ferro-lyase
    NP_000131.2
    NP_001012533.1

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    Genomic

    1. NG_008175.1 RefSeqGene

      Range
      5001..46897
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_000140.3NP_000131.2  ferrochelatase, mitochondrial isoform b precursor

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) uses an alternate in-frame donor splice site at one of the internal coding exons, compared to transcript variant 1. This results in a slightly shorter isoform (b) with the same N- and C- termini, but missing a 6 aa segment compared to isoform a.
      Source sequence(s)
      AC100847, AI767430, AJ709747, BC039841, BU734879, BX571744, DA990386
      Consensus CDS
      CCDS11964.1
      UniProtKB/Swiss-Prot
      P22830
      UniProtKB/TrEMBL
      Q7KZA3
      Conserved Domains (3) summary
      cd03411
      Location:69230
      Blast Score: 421
      Ferrochelatase_N; Ferrochelatase, N-terminal domain: Ferrochelatase (protoheme ferrolyase or HemH) is the terminal enzyme of the heme biosynthetic pathway. It catalyzes the insertion of ferrous iron into the protoporphyrin IX ring yielding protoheme. This enzyme is ...
      cd00419
      Location:235372
      Blast Score: 441
      Ferrochelatase_C; Ferrochelatase, C-terminal domain: Ferrochelatase (protoheme ferrolyase or HemH) is the terminal enzyme of the heme biosynthetic pathway. It catalyzes the insertion of ferrous iron into the protoporphyrin IX ring yielding protoheme. This enzyme is ...
      pfam00762
      Location:69389
      Blast Score: 1016
      Ferrochelatase; Ferrochelatase
    2. NM_001012515.2NP_001012533.1  ferrochelatase, mitochondrial isoform a precursor

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) represents the predominant transcript and encodes the longer isoform (a).
      Source sequence(s)
      AC100847, AI767430, AJ709747, AK092416, BC039841, BU734879, BX571744, DA990386
      Consensus CDS
      CCDS32836.1
      UniProtKB/Swiss-Prot
      P22830
      UniProtKB/TrEMBL
      Q7KZA3
      Conserved Domains (3) summary
      cd03411
      Location:75236
      Blast Score: 419
      Ferrochelatase_N; Ferrochelatase, N-terminal domain: Ferrochelatase (protoheme ferrolyase or HemH) is the terminal enzyme of the heme biosynthetic pathway. It catalyzes the insertion of ferrous iron into the protoporphyrin IX ring yielding protoheme. This enzyme is ...
      cd00419
      Location:241378
      Blast Score: 441
      Ferrochelatase_C; Ferrochelatase, C-terminal domain: Ferrochelatase (protoheme ferrolyase or HemH) is the terminal enzyme of the heme biosynthetic pathway. It catalyzes the insertion of ferrous iron into the protoporphyrin IX ring yielding protoheme. This enzyme is ...
      pfam00762
      Location:74395
      Blast Score: 1022
      Ferrochelatase; Ferrochelatase

    RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 104

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh37.p10 Primary Assembly

    Genomic

    1. NC_000018.9 Reference GRCh37.p10 Primary Assembly

      Range
      55212073..55253969, complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate HuRef

    Genomic

    1. AC_000150.1 Alternate HuRef

      Range
      51922248..51964183, complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate CHM1_1.0

    Genomic

    1. NC_018929.1 Alternate CHM1_1.0

      Range
      55065863..55107757, complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

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