Sphk1 sphingosine kinase 1 [Mus musculus (house mouse)] - Gene

Summary

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Official Symbol: Sphk1provided by MGI
Official Full Name: sphingosine kinase 1provided by MGI
Primary source: MGI:1316649
Locus tag: RP23-193A16.4
See related: Ensembl:ENSMUSG00000061878; Vega:OTTMUSG00000003900
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Sk1; Spk1; 1110006G24Rik
Summary: This gene encodes a kinase that phosphorylates sphingosine into sphingosine-1-phosphate, which is involved in cell differentiation, motility, and apoptosis. The encoded protein plays a role in maintaining cellular levels of sphingosine-1-phosphate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]

Genomic context

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Location :: 11 E2; 11

Sequence :: Chromosome: 11; NC_000077.6 (116531911..116536675)

See Sphk1 in Epigenomics, MapViewer

Chromosome 11 - NC_000077.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence

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Go to reference sequence details

Bibliography

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GeneRIFs: Gene References Into Functions What's a GeneRIF?

The function of SphK1 and SphK2 can be interchangeable in mast cells and is species and cell type determined.
Title: Usage of sphingosine kinase isoforms in mast cells is species and/or cell type determined.
SphK1 regulates PGE(2) production by mPGES-1 expression via the p38 MAPK pathway, independent of COX-2 signaling, in endothelial cells
Title: Effect of sphingosine kinase 1 inhibition on blood pressure.
The lipid kinase SphK1 plays a positive and essential role in the growth and development of malignant mesothelioma and is therefore a likely therapeutic target.
Title: Sphingosine kinase 1 is required for mesothelioma cell proliferation: role of histone acetylation.
these results reveal that SphK1/S1PR1 signalling axis is induced by TGF-beta1 and stimulates cell migration in RAW 264.7 macrophages.
Title: Sphingosine kinase-1/sphingosine-1-phosphate receptor type 1 signalling axis is induced by transforming growth factor-β1 and stimulates cell migration in RAW264.7 macrophages.
Oncogenic K-Ras regulates bioactive sphingolipids in a sphingosine kinase 1-dependent manner
Title: Oncogenic K-Ras regulates bioactive sphingolipids in a sphingosine kinase 1-dependent manner.
Thus, these data suggest that systemic SK1/sphingosine 1-phosphate regulates metastatic potential via regulation of tumour S1PR2/Brms1 axis.
Title: Communication between host organism and cancer cells is transduced by systemic sphingosine kinase 1/sphingosine 1-phosphate signalling to regulate tumour metastasis.
results suggest that SphK1 activation is involved in the regulation of LPS induced brain injury
Title: Sphingosine kinase 1 deficiency exacerbates LPS-induced neuroinflammation.
Genetic inhibition of SphK1 activity results in decreased expression of inflammatory mediators and decreased activation of inflammatory pathways in murine fibroblast-like synoviocytes.
Title: Impact of sphingosine kinase on inflammatory pathways in fibroblast-like synoviocytes.
targeting SK1 may restore sphingolipid homeostasis in p53-dependent tumors and provide insights into novel therapeutic approaches to cancer.
Title: Defining a role for sphingosine kinase 1 in p53-dependent tumors.
contributes to oral sensitization and effector phase in a mouse model of food allergy
Title: Sphingosine-kinase 1 and 2 contribute to oral sensitization and effector phase in a mouse model of food allergy.

Submit: New GeneRIF Correction See all (84)

Alleles

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Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (Floxed/Frt) (1) 1 citation
Targeted (knock-out) (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description
	NP_079647.1	Acy1		BIND	PubMed	SphK1 interacts with Acy1.

Pathways from BioSystems

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Association of TriC/CCT with target proteins during biosynthesis, organism-specific biosystem (from REACTOME)
Association of TriC/CCT with target proteins during biosynthesis, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Calcium signaling pathway, organism-specific biosystem (from KEGG)
Calcium signaling pathway, organism-specific biosystemCa2+ that enters the cell from the outside is a principal source of signal Ca2+. Entry of Ca2+ is driven by the presence of a large electrochemical gradient across the plasma membrane. Cells use this...
Calcium signaling pathway, conserved biosystem (from KEGG)
Calcium signaling pathway, conserved biosystemCa2+ that enters the cell from the outside is a principal source of signal Ca2+. Entry of Ca2+ is driven by the presence of a large electrochemical gradient across the plasma membrane. Cells use this...
Chaperonin-mediated protein folding, organism-specific biosystem (from REACTOME)
Chaperonin-mediated protein folding, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Fc gamma R-mediated phagocytosis, organism-specific biosystem (from KEGG)
Fc gamma R-mediated phagocytosis, organism-specific biosystemPhagocytosis plays an essential role in host-defense mechanisms through the uptake and destruction of infectious pathogens. Specialized cell types including macrophages, neutrophils, and monocytes ta...
Fc gamma R-mediated phagocytosis, conserved biosystem (from KEGG)
Fc gamma R-mediated phagocytosis, conserved biosystemPhagocytosis plays an essential role in host-defense mechanisms through the uptake and destruction of infectious pathogens. Specialized cell types including macrophages, neutrophils, and monocytes ta...
Metabolism, organism-specific biosystem (from REACTOME)
Metabolism, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Metabolism of lipids and lipoproteins, organism-specific biosystem (from REACTOME)
Metabolism of lipids and lipoproteins, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Metabolism of proteins, organism-specific biosystem (from REACTOME)
Metabolism of proteins, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Protein folding, organism-specific biosystem (from REACTOME)
Protein folding, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Signal Transduction of S1P Receptor, organism-specific biosystem (from WikiPathways)
Signal Transduction of S1P Receptor, organism-specific biosystem
Signal Transduction of S1P Receptor
Sphingolipid de novo biosynthesis, organism-specific biosystem (from REACTOME)
Sphingolipid de novo biosynthesis, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Sphingolipid metabolism, organism-specific biosystem (from KEGG)
Sphingolipid metabolism, organism-specific biosystem
Sphingolipid metabolism
Sphingolipid metabolism, organism-specific biosystem (from REACTOME)
Sphingolipid metabolism, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Sphingolipid metabolism, conserved biosystem (from KEGG)
Sphingolipid metabolism, conserved biosystem
Sphingolipid metabolism
Sphingolipids metabolism pathway, organism-specific biosystem (from LIPID MAPS)
Sphingolipids metabolism pathway, organism-specific biosystem
Sphingolipids metabolism pathway
Sphingosine degradation, organism-specific biosystem (from KEGG)
Sphingosine degradation, organism-specific biosystemPathway module; Carbohydrate and lipid metabolism; Lipid metabolism
Sphingosine degradation, conserved biosystem (from KEGG)
Sphingosine degradation, conserved biosystemPathway module; Carbohydrate and lipid metabolism; Lipid metabolism
Tuberculosis, organism-specific biosystem (from KEGG)
Tuberculosis, organism-specific biosystemTuberculosis, or TB, is an infectious disease caused by Mycobacterium tuberculosis. One third of the world's population is thought to be infected with TB. About 90% of those infected result in latent...
Tuberculosis, conserved biosystem (from KEGG)
Tuberculosis, conserved biosystemTuberculosis, or TB, is an infectious disease caused by Mycobacterium tuberculosis. One third of the world's population is thought to be infected with TB. About 90% of those infected result in latent...
VEGF signaling pathway, organism-specific biosystem (from KEGG)
VEGF signaling pathway, organism-specific biosystemThere is now much evidence that VEGFR-2 is the major mediator of VEGF-driven responses in endothelial cells and it is considered to be a crucial signal transducer in both physiologic and pathologic a...
VEGF signaling pathway, conserved biosystem (from KEGG)
VEGF signaling pathway, conserved biosystemThere is now much evidence that VEGFR-2 is the major mediator of VEGF-driven responses in endothelial cells and it is considered to be a crucial signal transducer in both physiologic and pathologic a...
sphingosine and sphingosine-1-phosphate metabolism, organism-specific biosystem (from BIOCYC)
sphingosine and sphingosine-1-phosphate metabolism, organism-specific biosystemIn this pathway, ceramide, the breakdown product of sphingomyelin, is metabolized by ceramidase to generate sphingosine, which has been shown to be produced during the early stages of apoptosis; sphi...
sphingosine and sphingosine-1-phosphate metabolism, conserved biosystem (from BIOCYC)
sphingosine and sphingosine-1-phosphate metabolism, conserved biosystemIn this pathway, |FRAME: Ceramides ceramides"|, the breakdown products of |FRAME: Sphingomyelins sphingomyelins|, are metabolized by ceramidase to generate |FRAME: SPHINGOSINE|, which has been shown ...

General gene information

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Markers

Sphk1 (e-PCR), detects polymorphism
- UniSTS:496531

NoName (e-PCR)
- UniSTS:236783

Homology

Homologs of the Sphk1 gene: The Sphk1 gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, rat, chicken, zebrafish, A.thaliana, and rice.
Map Viewer (Human, Rat)
OrthoDB: The Hierarchical Catalog of Eukaryotic Orthologs

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
ATP binding	ISO Inferred from Sequence Orthology more info
D-erythro-sphingosine kinase activity	ISO Inferred from Sequence Orthology more info
DNA binding	ISA Inferred from Sequence Alignment more info	PubMed
calmodulin binding	ISA Inferred from Sequence Alignment more info	PubMed
calmodulin binding	ISO Inferred from Sequence Orthology more info
diacylglycerol kinase activity	IEA Inferred from Electronic Annotation more info
kinase activity	IEA Inferred from Electronic Annotation more info
magnesium ion binding	ISO Inferred from Sequence Orthology more info
nucleotide binding	IEA Inferred from Electronic Annotation more info
protein phosphatase 2A binding	ISO Inferred from Sequence Orthology more info
sphinganine kinase activity	ISA Inferred from Sequence Alignment more info	PubMed
sphinganine kinase activity	ISO Inferred from Sequence Orthology more info
transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
blood vessel development	IGI Inferred from Genetic Interaction more info	PubMed
brain development	IGI Inferred from Genetic Interaction more info	PubMed
calcium-mediated signaling	ISO Inferred from Sequence Orthology more info
cyclooxygenase pathway	ISO Inferred from Sequence Orthology more info
inflammatory response	IMP Inferred from Mutant Phenotype more info	PubMed
lipid phosphorylation	ISA Inferred from Sequence Alignment more info	PubMed
lipid phosphorylation	ISO Inferred from Sequence Orthology more info
negative regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
phosphorylation	IEA Inferred from Electronic Annotation more info
positive regulation of NF-kappaB import into nucleus	ISO Inferred from Sequence Orthology more info
positive regulation of NF-kappaB transcription factor activity	ISO Inferred from Sequence Orthology more info
positive regulation of angiogenesis	ISO Inferred from Sequence Orthology more info
positive regulation of cell growth	ISO Inferred from Sequence Orthology more info
positive regulation of cell migration	ISO Inferred from Sequence Orthology more info
positive regulation of cell proliferation	IGI Inferred from Genetic Interaction more info	PubMed
positive regulation of fibroblast proliferation	ISO Inferred from Sequence Orthology more info
positive regulation of mitotic cell cycle	ISO Inferred from Sequence Orthology more info
positive regulation of neuron projection development	ISO Inferred from Sequence Orthology more info
positive regulation of neurotransmitter secretion	ISO Inferred from Sequence Orthology more info
positive regulation of protein phosphorylation	ISO Inferred from Sequence Orthology more info
positive regulation of protein ubiquitination	ISO Inferred from Sequence Orthology more info
positive regulation of smooth muscle contraction	ISO Inferred from Sequence Orthology more info
protein kinase C-activating G-protein coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
regulation of interleukin-1 beta production	IMP Inferred from Mutant Phenotype more info	PubMed
regulation of tumor necrosis factor-mediated signaling pathway	ISO Inferred from Sequence Orthology more info
sphingosine metabolic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
axon	ISO Inferred from Sequence Orthology more info
cytoplasm	ISO Inferred from Sequence Orthology more info
cytosol	ISO Inferred from Sequence Orthology more info
neuron projection	ISO Inferred from Sequence Orthology more info
nucleus	IEA Inferred from Electronic Annotation more info
plasma membrane	ISO Inferred from Sequence Orthology more info
synaptic vesicle	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: sphingosine kinase 1

Names: sphingosine kinase 1; SK 1; SPK 1

NP_001165943.1

EC 2.7.1.91

NP_001165944.1

EC 2.7.1.91

NP_001165946.1

EC 2.7.1.91

NP_035581.1

EC 2.7.1.91

NP_079643.2

EC 2.7.1.91

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001172472.1 → NP_001165943.1 sphingosine kinase 1 isoform 2

Status: REVIEWED

Description

Transcript Variant: This variant (4) differs in the 5' UTR, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Variants 2, 3 and 4 encode the same protein (isoform 2). This isoform is also known as Sphk1a2.

Source sequence(s)

AL645851

Consensus CDS

CCDS25668.1

UniProtKB/Swiss-Prot

Q8CI15

Related

ENSMUSP00000101994, OTTMUSP00000004203, ENSMUST00000106386, OTTMUST00000008630

Conserved Domains (2) summary

COG1597
Location:22 – 358
Blast Score: 227

LCB5; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase [Lipid metabolism / General function prediction only]

pfam00781
Location:22 – 145
Blast Score: 256

DAGK_cat; Diacylglycerol kinase catalytic domain
NM_001172473.1 → NP_001165944.1 sphingosine kinase 1 isoform 2

Status: REVIEWED

Description

Transcript Variant: This variant (3) differs in the 5' UTR, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Variants 2, 3 and 4 encode the same protein (isoform 2). This isoform is also known as Sphk1a2.

Source sequence(s)

AK160900, AL645851

Consensus CDS

CCDS25668.1

UniProtKB/Swiss-Prot

Q8CI15

Conserved Domains (2) summary

COG1597
Location:22 – 358
Blast Score: 227

LCB5; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase [Lipid metabolism / General function prediction only]

pfam00781
Location:22 – 145
Blast Score: 256

DAGK_cat; Diacylglycerol kinase catalytic domain
NM_001172475.1 → NP_001165946.1 sphingosine kinase 1 isoform 3

Status: REVIEWED

Description

Transcript Variant: This variant (5) differs in the 5' UTR and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is shorter than isoform 1. This isoform is also known as Sphk1a.

Source sequence(s)

AL645851

Consensus CDS

CCDS48986.1

UniProtKB/Swiss-Prot

Q8CI15

Related

ENSMUSP00000097775, OTTMUSP00000004200, ENSMUST00000100201, OTTMUST00000008627

Conserved Domains (2) summary

COG1597
Location:24 – 357
Blast Score: 213

LCB5; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase [Lipid metabolism / General function prediction only]

pfam00781
Location:23 – 144
Blast Score: 239

DAGK_cat; Diacylglycerol kinase catalytic domain
NM_011451.3 → NP_035581.1 sphingosine kinase 1 isoform 1 precursor

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the shortest transcript and encodes the longest isoform (1). This isoform is also known as Sphk1b.

Source sequence(s)

AL645851

Consensus CDS

CCDS25669.1

UniProtKB/Swiss-Prot

Q8CI15

Related

ENSMUSP00000131010, OTTMUSP00000051871, ENSMUST00000141798, OTTMUST00000008633

Conserved Domains (2) summary

COG1597
Location:28 – 364
Blast Score: 231

LCB5; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase [Lipid metabolism / General function prediction only]

pfam00781
Location:28 – 151
Blast Score: 257

DAGK_cat; Diacylglycerol kinase catalytic domain
NM_025367.6 → NP_079643.2 sphingosine kinase 1 isoform 2

Status: REVIEWED

Description

Transcript Variant: This variant (2) differs in the 5' UTR, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Variants 2, 3 and 4 encode the same protein (isoform 2). This isoform is also known as Sphk1a2.

Source sequence(s)

AL645851

Consensus CDS

CCDS25668.1

UniProtKB/Swiss-Prot

Q8CI15

Conserved Domains (2) summary

COG1597
Location:22 – 358
Blast Score: 227

LCB5; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase [Lipid metabolism / General function prediction only]

pfam00781
Location:22 – 145
Blast Score: 256

DAGK_cat; Diacylglycerol kinase catalytic domain

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 103

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm38.p1 C57BL/6J

Genomic

NC_000077.6 Reference GRCm38.p1 C57BL/6J

Range

116531911..116536675

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate Mm_Celera

Genomic

AC_000033.1 Alternate Mm_Celera

Range

128300450..128305214

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Nucleotide		Protein	Strain
Heading	Accession and Version	Protein	Strain
genomic	AAHY01096732.1 (15456..20220)	None
genomic	AL645851.8 (69520..74285)	None
genomic	CH466558.1	EDL34581.1	mixed
		EDL34582.1	mixed
		EDL34583.1	mixed
mRNA	AF068748.1	AAC61697.1
mRNA	AF068749.1	AAC61698.1
mRNA	AF415213.1	AAL07499.1
mRNA	AK028667.1	None	C57BL/6J
mRNA	AK136289.1	BAE22918.1	C57BL/6J
mRNA	AK155332.1	BAE33198.1	NOD
mRNA	AK160900.1	BAE36079.1	C57BL/6J
mRNA	AK197304.1	None
mRNA	AK216707.1	None
mRNA	AK218698.1	None
mRNA	BC011432.1	None	FVB/N
mRNA	BC037710.1	AAH37710.1	FVB/N