Sp3 trans-acting transcription factor 3 [Mus musculus (house mouse)] - Gene

Summary

Go to the top of the page Help

Official Symbol: Sp3provided by MGI
Official Full Name: trans-acting transcription factor 3provided by MGI
Primary source: MGI:1277166
See related: Ensembl:ENSMUSG00000027109; Vega:OTTMUSG00000013207
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: D130027J01Rik
Summary: This gene product belongs to a family of Sp1 related transcription factors, which regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses transcription of numerous genes. Alternative splicing results in transcript variants encoding different isoforms, and one variant initiates translation from a non-AUG (AUA) codon. [provided by RefSeq, Jul 2008]

Genomic context

Go to the top of the page Help

Location :: 2 C3; 2

Sequence :: Chromosome: 2; NC_000068.7 (72936430..72980446, complement)

See Sp3 in Epigenomics, MapViewer

Chromosome 2 - NC_000068.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Three GC boxes within the proximal promoter of the murine pyruvate carboxylase gene bind Sp1/Sp3
Title: Functional characterization of the proximal promoter of the murine pyruvate carboxylase gene in hepatocytes: role of multiple gc boxes.
Results suggest that Sp3 and YY1 recruit general transcription factors and facilitate the assembly of a preinitiation complex.
Title: Constitutive expression of the brg1 gene requires GC-boxes near to the transcriptional start site.
These results establish SUMO conjugation to Sp3 as a molecular beacon for the assembly of repression machineries to maintain tissue-specific transcriptional gene silencing.
Title: Epigenetic silencing of spermatocyte-specific and neuronal genes by SUMO modification of the transcription factor Sp3.
Sp3 was a dose-dependent repressor of Sp1 and PTH-induced matrix gla protein expression
Title: Sp proteins and Runx2 mediate regulation of matrix gla protein (MGP) expression by parathyroid hormone.
Sp1 and Sp3 regulate transcription of the cyclin-dependent kinase 5 regulatory subunit 2 (p39) promoter in neuronal cells.
Title: Sp1 and Sp3 regulate transcription of the cyclin-dependent kinase 5 regulatory subunit 2 (p39) promoter in neuronal cells.
Studies suggest that the expression of the ldh2 gene in spermatogonia and early spermatocytes are regulated by SP-mediated transcriptional mechanisms.
Title: Molecular and functional characterization of the murine ldh2 promoter region: Sp-binding GC-box domains are the key cis-elements regulating ldh2 gene expression during spermatogenesis.
Expression patterns of SP1 and SP3 during mouse spermatogenesis.
Title: Expression patterns of SP1 and SP3 during mouse spermatogenesis: SP1 down-regulation correlates with two successive promoter changes and translationally compromised transcripts.
Sp3 is required for normal cardiac development and suggest that it has a crucial role in myocardial differentiation.
Title: Transcription factor Sp3 knockout mice display serious cardiac malformations.
Methylglyoxal modification of mSin3A results in increased modification of Sp3 by O-linked N-acetylglucosamine. This modification of Sp3 causes decreased binding to angiopoietin-2 (Ang-2) promoter, resulting in increased Ang-2 expression.
Title: High glucose increases angiopoietin-2 transcription in microvascular endothelial cells through methylglyoxal modification of mSin3A.
a threshold of Sp1 and Sp3 activity is required for normal embryonic development, suggesting that Sp1 and Sp3 act cooperatively to regulate downstream targets
Title: Sp1/Sp3 compound heterozygous mice are not viable: impaired erythropoiesis and severe placental defects.

Submit: New GeneRIF Correction See all (31)

Alleles

Go to the top of the page Help

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (Floxed/Frt) (1) 1 citation
Targeted (Reporter) (1) 1 citation
Targeted (knock-in) (1) 1 citation

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Source	Pubs	Description
NP_035580.1	NP_034979.2	Myc	BIND	PubMed	Myc interacts with Sp3.
BioGRID:203418	BioGRID:241423	Hdac1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:203418	BioGRID:200260	Hdac2	BioGRID	PubMed	Affinity Capture-Western
BioGRID:203418	BioGRID:202864	Rest	BioGRID	PubMed	Affinity Capture-Western
BioGRID:203418	BioGRID:203256	Sin3a	BioGRID	PubMed	Affinity Capture-Western
BioGRID:203418	BioGRID:204420	Sumo1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:203418	BioGRID:203978	Tbp	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex
BioGRID:203418	BioGRID:204624	Yy1	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex

Pathways from BioSystems

Go to the top of the page Help

PluriNetWork, organism-specific biosystem (from WikiPathways)
PluriNetWork, organism-specific biosystemThe aim of the PluriNetWork is to give an overview of specific mechanisms associated with pluripotency in mouse. Each node represents a gene and its corresponding protein product. As stated, we inten...
Selenium metabolism/Selenoproteins, organism-specific biosystem (from WikiPathways)
Selenium metabolism/Selenoproteins, organism-specific biosystem
Selenium metabolism/Selenoproteins

General gene information

Go to the top of the page Help

Markers

D10Mit157.2 (e-PCR), detects polymorphism
- UniSTS:126061

PMC305603P1 (e-PCR)
- UniSTS:272552

BE198984 (e-PCR)
- UniSTS:207344

PMC169877P1 (e-PCR)
- UniSTS:271610

Homology

Homologs of the Sp3 gene: The Sp3 gene is conserved in human, Rhesus monkey, dog, cow, rat, chicken, and zebrafish.
Map Viewer (Human, Rat)
OrthoDB: The Hierarchical Catalog of Eukaryotic Orthologs

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
DNA binding	IDA Inferred from Direct Assay more info	PubMed
DNA binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription	IDA Inferred from Direct Assay more info	PubMed
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription	ISO Inferred from Sequence Orthology more info
RNA polymerase II core promoter sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
chromatin binding	IDA Inferred from Direct Assay more info	PubMed
core promoter proximal region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
double-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
metal ion binding	IEA Inferred from Electronic Annotation more info
nucleic acid binding	IEA Inferred from Electronic Annotation more info
protein binding	IPI Inferred from Physical Interaction more info	PubMed
sequence-specific DNA binding transcription factor activity	TAS Traceable Author Statement more info	PubMed
zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
B cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
T cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
definitive hemopoiesis	IGI Inferred from Genetic Interaction more info	PubMed
embryonic camera-type eye morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
embryonic placenta development	IGI Inferred from Genetic Interaction more info	PubMed
embryonic process involved in female pregnancy	IGI Inferred from Genetic Interaction more info	PubMed
embryonic skeletal system development	IGI Inferred from Genetic Interaction more info	PubMed
enucleate erythrocyte differentiation	IGI Inferred from Genetic Interaction more info	PubMed
erythrocyte differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
granulocyte differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
in utero embryonic development	IGI Inferred from Genetic Interaction more info	PubMed
liver development	IGI Inferred from Genetic Interaction more info	PubMed
lung development	IGI Inferred from Genetic Interaction more info	PubMed
megakaryocyte differentiation	IGI Inferred from Genetic Interaction more info	PubMed
monocyte differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
natural killer cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
negative regulation of transcription from RNA polymerase II promoter	IDA Inferred from Direct Assay more info	PubMed
negative regulation of transcription, DNA-dependent	IDA Inferred from Direct Assay more info	PubMed
negative regulation of transcription, DNA-dependent	ISO Inferred from Sequence Orthology more info
ossification	IGI Inferred from Genetic Interaction more info	PubMed
positive regulation of transcription, DNA-dependent	ISO Inferred from Sequence Orthology more info
regulation of transcription, DNA-dependent	ISO Inferred from Sequence Orthology more info
regulation of transcription, DNA-dependent	TAS Traceable Author Statement more info	PubMed
transcription, DNA-dependent	IEA Inferred from Electronic Annotation more info
trophectodermal cell differentiation	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
nucleus	IDA Inferred from Direct Assay more info	PubMed
transcriptional repressor complex	IDA Inferred from Direct Assay more info	PubMed
transcriptional repressor complex	ISO Inferred from Sequence Orthology more info

General protein information

Go to the top of the page Help

Preferred Names: transcription factor Sp3

Names: transcription factor Sp3

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001018042.3 → NP_001018052.1 transcription factor Sp3 isoform 1

Status: REVIEWED

Description

Transcript Variant: This variant (1) encodes the longer isoform (1).

Source sequence(s)

BC079874

Consensus CDS

CCDS16122.1

UniProtKB/Swiss-Prot

O70494

Related

ENSMUSP00000099750, OTTMUSP00000014207, ENSMUST00000102689, OTTMUST00000031922

Conserved Domains (2) summary

smart00355
Location:683 – 705
Blast Score: 95

ZnF_C2H2; zinc finger

pfam13465
Location:669 – 692
Blast Score: 129

zf-H2C2_2; Zinc-finger double domain
NM_001098425.1 → NP_001091895.1 transcription factor Sp3 isoform 2

Status: REVIEWED

Description

Transcript Variant: This variant (2) is missing 2 exons from the 5' end compared to transcript variant 1, and it initiates translation from an in-frame, downstream non-AUG (AUA) codon, resulting in an isoform (2) with a shorter N-terminus compared to isoform 1.

Source sequence(s)

BC079874, CA328978, CJ167744

UniProtKB/Swiss-Prot

O70494

Conserved Domains (2) summary

smart00355
Location:616 – 638
Blast Score: 95

ZnF_C2H2; zinc finger

pfam13465
Location:602 – 625
Blast Score: 128

zf-H2C2_2; Zinc-finger double domain

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 103

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm38.p1 C57BL/6J

Genomic

NC_000068.7 Reference GRCm38.p1 C57BL/6J

Range

72936430..72980446, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate Mm_Celera

Genomic

AC_000024.1 Alternate Mm_Celera

Range

74621937..74665927, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

NM_011450.1: Suppressed sequence

Description

NM_011450.1: This RefSeq was permanently suppressed because currently there is insufficient support for the transcript and the protein.

Nucleotide		Protein	Strain
Heading	Accession and Version	Protein	Strain
genomic	AAHY01017279.1 (31807..43319)	None
genomic	AAHY01017280.1	None
genomic	AAHY01017281.1	None
genomic	AAHY01017282.1	None
genomic	AL844840.8 (142755..186771)	None
genomic	AY902330.1	AAX90615.1	CAST/EiJ
genomic	CH466519.1	EDL27117.1	mixed
		EDL27118.1	mixed
mRNA	AF062567.1	AAC16322.1
mRNA	AK004607.1	BAC25090.1	C57BL/6J
mRNA	AK005209.1	None	C57BL/6J
mRNA	AK047030.1	None	C57BL/6J
mRNA	AK051273.1	None	C57BL/6J
mRNA	AK081759.1	BAC38323.1	C57BL/6J
mRNA	AK086963.1	None	C57BL/6J
mRNA	AK088238.1	None	NOD
mRNA	AK132702.1	BAE21310.1	C57BL/6J
mRNA	BC027797.1	AAH27797.2	FVB/N
mRNA	BC079874.1	AAH79874.1	C57BL/6
mRNA	CA328978.1	None	C57BL/6
mRNA	CJ167744.1	None