EPHB3 EPH receptor B3 [Homo sapiens (human)] - Gene

Summary

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Official Symbol: EPHB3provided by HGNC
Official Full Name: EPH receptor B3provided by HGNC
Primary source: HGNC:3394
See related: Ensembl:ENSG00000182580; HPRD:03502; MIM:601839; Vega:OTTHUMG00000156710
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: ETK2; HEK2; TYRO6
Summary: Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into two groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. This gene encodes a receptor for ephrin-B family members. [provided by RefSeq, Mar 2010]

Genomic context

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Location :: 3q21-qter

Sequence :: Chromosome: 3; NC_000003.11 (184279587..184300196)

See EPHB3 in Epigenomics, MapViewer

Chromosome 3 - NC_000003.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

Bibliography

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GeneRIFs: Gene References Into Functions What's a GeneRIF?

EphB3 suppresses non-small-cell lung cancer metastasis via a PP2A/RACK1/Akt signalling complex
Title: EphB3 suppresses non-small-cell lung cancer metastasis via a PP2A/RACK1/Akt signalling complex.
Our work shows that EphB3 is consistently expressed by malignant T lymphocytes, most frequently in combination with EphB6, and that stimulation with their common ligands strongly suppresses Fas-induced apoptosis in these cells.
Title: EphB receptors trigger Akt activation and suppress Fas receptor-induced apoptosis in malignant T lymphocytes.
Data show that EphB receptors interact with E-cadherin and with the metalloproteinase ADAM10 at sites of adhesion.
Title: Cleavage of E-cadherin by ADAM10 mediates epithelial cell sorting downstream of EphB signalling.
EphB3 provides critical support to the development and progression of NSCLC by stimulating cell growth, migration, and survival.
Title: EphB3 is overexpressed in non-small-cell lung cancer and promotes tumor metastasis by enhancing cell survival and migration.
Ephrin B3 receptor regulates the synthesis and release of D-serine in astrocytes, which may have important implications on synaptic transmission and plasticity.
Title: EphrinBs regulate D-serine synthesis and release in astrocytes.
EPHB3, MASP1 and SST map to 3q26.2-q29 and may have roles in squamous cell carcinoma of the lung
Title: Identification of novel candidate target genes, including EPHB3, MASP1 and SST at 3q26.2-q29 in squamous cell carcinoma of the lung.
EphB3-ephrin-B interaction promotes mesenchymal-to-epithelial transition (MET) by re-establishing epithelial cell-cell junctions and such an MET-promoting effect contributes to EphB3-mediated tumor suppression.
Title: Over-expression of EphB3 enhances cell-cell contacts and suppresses tumor growth in HT-29 human colon cancer cells.
Observational study of gene-disease association. (HuGE Navigator)
Title: Maternal genes and facial clefts in offspring: a comprehensive search for genetic associations in two population-based cleft studies from Scandinavia.
RYK, a catalytically inactive receptor tyrosine kinase, associates with EphB2 and EphB3 but does not interact with AF-6.
Title: RYK, a catalytically inactive receptor tyrosine kinase, associates with EphB2 and EphB3 but does not interact with AF-6.
while catalytic activity of EphB3 is required for inhibition of integrin-mediated cell adhesion, a distinct signaling pathway to Rho GTPases shared by WT- and KD-EphB3 receptor mediates inhibition of directional cell migration
Title: Inhibition of integrin-mediated cell adhesion but not directional cell migration requires catalytic activity of EphB3 receptor tyrosine kinase. Role of Rho family small GTPases.

Submit: New GeneRIF Correction

Phenotypes

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Review eQTL and phenotype association data in this region using PheGenI

Interactions

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Products	Interactant	Other Gene	Source	Pubs	Description
NP_004434.2	NP_005927.1	MLLT4	BIND	PubMed	EphB3 interacts with AF6. This interaction was modelled on a demonstrated interaction between EphB3 from human and mouse AF6.
P54753	P46108	CRK	HPRD	PubMed
P54753	Q15768	EFNB3	HPRD	PubMed
P54753	P54753	EPHB3	HPRD	PubMed
P54753	P06241	FYN	HPRD	PubMed
P54753	P55196	MLLT4	HPRD	PubMed
P54753	P20936	RASA1	HPRD	PubMed
P54753	P34925	RYK	HPRD	PubMed
BioGRID:108363	BioGRID:107788	CRK	BioGRID	PubMed	Reconstituted Complex
BioGRID:108363	BioGRID:108269	EFNB3	BioGRID	PubMed	Reconstituted Complex
BioGRID:108363	BioGRID:108810	FYN	BioGRID	PubMed	Reconstituted Complex
BioGRID:108363	BioGRID:108902	GBE1	BioGRID	PubMed	Two-hybrid
BioGRID:108363	BioGRID:124219	LNX1	BioGRID	PubMed	Reconstituted Complex
BioGRID:108363	BioGRID:110447	MLLT4	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex; Two-hybrid
BioGRID:108363	BioGRID:110864	NFRKB	BioGRID	PubMed	Two-hybrid
BioGRID:108363	BioGRID:111856	RASA1	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex
BioGRID:108363	BioGRID:112171	RYK	BioGRID	PubMed	Affinity Capture-Western
BioGRID:108363	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS

Pathways from BioSystems

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Axon guidance, organism-specific biosystem (from KEGG)
Axon guidance, organism-specific biosystemAxon guidance represents a key stage in the formation of neuronal network. Axons are guided by a variety of guidance factors, such as netrins, ephrins, Slits, and semaphorins. These guidance cues are...
Axon guidance, conserved biosystem (from KEGG)
Axon guidance, conserved biosystemAxon guidance represents a key stage in the formation of neuronal network. Axons are guided by a variety of guidance factors, such as netrins, ephrins, Slits, and semaphorins. These guidance cues are...
EPHB forward signaling, organism-specific biosystem (from Pathway Interaction Database)
EPHB forward signaling, organism-specific biosystem
EPHB forward signaling
EphrinB-EPHB pathway, organism-specific biosystem (from Pathway Interaction Database)
EphrinB-EPHB pathway, organism-specific biosystem
EphrinB-EPHB pathway

General gene information

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Markers

SHGC-149120 (e-PCR)
- UniSTS:173108

RH70256 (e-PCR)
- UniSTS:9039

G15957 (e-PCR)
- UniSTS:16990

D3S3583 (e-PCR), detects polymorphism
- UniSTS:71112

EPHB3_445 (e-PCR)
- UniSTS:277213

D3S4004 (e-PCR)
- UniSTS:24049

SHGC-148412 (e-PCR)
- UniSTS:176380

Homology

Homologs of the EPHB3 gene: The EPHB3 gene is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, and zebrafish.
Map Viewer (Mouse, Rat)
OrthoDB: The Hierarchical Catalog of Eukaryotic Orthologs

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
ATP binding	IEA Inferred from Electronic Annotation more info
axon guidance receptor activity	IEA Inferred from Electronic Annotation more info
ephrin receptor activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
angiogenesis	ISS Inferred from Sequence or Structural Similarity more info
axon guidance	ISS Inferred from Sequence or Structural Similarity more info
axonal fasciculation	ISS Inferred from Sequence or Structural Similarity more info
cell migration	IDA Inferred from Direct Assay more info	PubMed
central nervous system projection neuron axonogenesis	IEA Inferred from Electronic Annotation more info
corpus callosum development	ISS Inferred from Sequence or Structural Similarity more info
dendritic spine development	ISS Inferred from Sequence or Structural Similarity more info
dendritic spine morphogenesis	ISS Inferred from Sequence or Structural Similarity more info
digestive tract morphogenesis	ISS Inferred from Sequence or Structural Similarity more info
ephrin receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
palate development	ISS Inferred from Sequence or Structural Similarity more info
positive regulation of synapse assembly	ISS Inferred from Sequence or Structural Similarity more info
protein autophosphorylation	IDA Inferred from Direct Assay more info	PubMed
regulation of Cdc42 GTPase activity	IDA Inferred from Direct Assay more info	PubMed
regulation of Rac GTPase activity	IDA Inferred from Direct Assay more info	PubMed
regulation of axonogenesis	ISS Inferred from Sequence or Structural Similarity more info
regulation of cell-cell adhesion	IDA Inferred from Direct Assay more info	PubMed
retinal ganglion cell axon guidance	IEA Inferred from Electronic Annotation more info
substrate adhesion-dependent cell spreading	IDA Inferred from Direct Assay more info	PubMed
thymus development	ISS Inferred from Sequence or Structural Similarity more info
urogenital system development	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
dendrite	IEA Inferred from Electronic Annotation more info
integral to plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: ephrin type-B receptor 3

Names: ephrin type-B receptor 3; EK2; EPH-like kinase 2; embryonic kinase 2; human embryo kinase 2; EPH-like tyrosine kinase 2; EPH-like tyrosine kinase-2; tyrosine-protein kinase TYRO6

NP_004434.2

EC 2.7.10.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_004443.3 → NP_004434.2 ephrin type-B receptor 3 precursor

Status: REVIEWED

Source sequence(s)

BC052968, CB993133, X75208

Consensus CDS

CCDS3268.1

UniProtKB/Swiss-Prot

P54753

Related

ENSP00000332118, OTTHUMP00000210455, ENST00000330394, OTTHUMT00000345413

Conserved Domains (7) summary

cd00063
Location:473 – 542
Blast Score: 144

FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...

pfam07699
Location:284 – 316
Blast Score: 110

GCC2_GCC3; GCC2 and GCC3

cd05065
Location:628 – 896
Blast Score: 1567

PTKc_EphR_B; Catalytic domain of the Protein Tyrosine Kinases, Class EphB Ephrin Receptors

cd09553
Location:922 – 990
Blast Score: 372

SAM_EPH-B3; SAM domain of EPH-B3 subfamily of tyrosine kinase receptors

cd10478
Location:39 – 211
Blast Score: 964

EphR_LBD_B3; Ligand Binding Domain of Ephrin type-B Receptor 3

pfam07647
Location:922 – 987
Blast Score: 233

SAM_2; SAM domain (Sterile alpha motif)

pfam07714
Location:633 – 892
Blast Score: 1049

Pkinase_Tyr; Protein tyrosine kinase

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 104

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p10 Primary Assembly

Genomic

NC_000003.11 Reference GRCh37.p10 Primary Assembly

Range

184279587..184300196

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000135.1 Alternate HuRef

Range

181691476..181712092

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate CHM1_1.0

Genomic

NC_018914.1 Alternate CHM1_1.0

Range

184149748..184170360

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	ABBA01058435.1 (43694..64310)	None
genomic	AC112643.12 (30465..51074)	None
genomic	AMYH01013724.1 (5994..26606)	None
genomic	CH471052.2	EAW78236.1
		EAW78237.1
genomic	GM868977.1	CAT16951.1
mRNA	BC052968.1	AAH52968.1
mRNA	CB993133.1	None
mRNA	X75208.1	CAA53021.1