Gls2 glutaminase 2 (liver, mitochondrial) [Rattus norvegicus (Norway rat)] - Gene

Summary

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Official Symbol: Gls2provided by RGD
Official Full Name: glutaminase 2 (liver, mitochondrial)provided by RGD
Primary source: RGD:620359
See related: Ensembl:ENSRNOG00000031612
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus
Also known as: Ga
Summary: first enzyme of glutamine catabolism in liver [RGD, Feb 2006]

Genomic context

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Location :: 7q11

Sequence :: Chromosome: 7; NC_005106.3 (2589839..2600725)

See Gls2 in MapViewer

Chromosome 7 - NC_005106.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

Bibliography

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GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study demonstrated expression of alternative transcripts of the mammalian Gls2 gene. Transcriptional mechanisms giving rise to GLS2 variants and isolation of novel GLS2 transcripts in human, rat and mouse are presented.
Title: Mammalian glutaminase Gls2 gene encodes two functional alternative transcripts by a surrogate promoter usage mechanism.

Submit: New GeneRIF Correction

Pathways from BioSystems

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Alanine, aspartate and glutamate metabolism, organism-specific biosystem (from KEGG)
Alanine, aspartate and glutamate metabolism, organism-specific biosystem
Alanine, aspartate and glutamate metabolism
Alanine, aspartate and glutamate metabolism, conserved biosystem (from KEGG)
Alanine, aspartate and glutamate metabolism, conserved biosystem
Alanine, aspartate and glutamate metabolism
Amino acid synthesis and interconversion (transamination), organism-specific biosystem (from REACTOME)
Amino acid synthesis and interconversion (transamination), organism-specific biosystemcomputationally inferred pathway (not manually curated)
Arginine and proline metabolism, organism-specific biosystem (from KEGG)
Arginine and proline metabolism, organism-specific biosystem
Arginine and proline metabolism
Arginine and proline metabolism, conserved biosystem (from KEGG)
Arginine and proline metabolism, conserved biosystem
Arginine and proline metabolism
D-Glutamine and D-glutamate metabolism, organism-specific biosystem (from KEGG)
D-Glutamine and D-glutamate metabolism, organism-specific biosystem
D-Glutamine and D-glutamate metabolism
D-Glutamine and D-glutamate metabolism, conserved biosystem (from KEGG)
D-Glutamine and D-glutamate metabolism, conserved biosystem
D-Glutamine and D-glutamate metabolism
GABAergic synapse, organism-specific biosystem (from KEGG)
GABAergic synapse, organism-specific biosystemGamma aminobutyric acid (GABA) is the most abundant inhibitory neurotransmitter in the mammalian central nervous system (CNS). When released in the synaptic cleft, GABA binds to three major classes o...
GABAergic synapse, conserved biosystem (from KEGG)
GABAergic synapse, conserved biosystemGamma aminobutyric acid (GABA) is the most abundant inhibitory neurotransmitter in the mammalian central nervous system (CNS). When released in the synaptic cleft, GABA binds to three major classes o...
Glutamate Neurotransmitter Release Cycle, organism-specific biosystem (from REACTOME)
Glutamate Neurotransmitter Release Cycle, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Glutamatergic synapse, organism-specific biosystem (from KEGG)
Glutamatergic synapse, organism-specific biosystemGlutamate is the major excitatory neurotransmitter in the mammalian central nervous system(CNS). Glutamate is packaged into synaptic vesicles in the presynaptic terminal. Once released into the synap...
Glutamatergic synapse, conserved biosystem (from KEGG)
Glutamatergic synapse, conserved biosystemGlutamate is the major excitatory neurotransmitter in the mammalian central nervous system(CNS). Glutamate is packaged into synaptic vesicles in the presynaptic terminal. Once released into the synap...
Metabolism, organism-specific biosystem (from REACTOME)
Metabolism, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Metabolism of amino acids and derivatives, organism-specific biosystem (from REACTOME)
Metabolism of amino acids and derivatives, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Neuronal System, organism-specific biosystem (from REACTOME)
Neuronal System, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Neurotransmitter Release Cycle, organism-specific biosystem (from REACTOME)
Neurotransmitter Release Cycle, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Proximal tubule bicarbonate reclamation, organism-specific biosystem (from KEGG)
Proximal tubule bicarbonate reclamation, organism-specific biosystemOne of the major tasks of the renal proximal tubule (PT) is to secrete acid into the tubule lumen, thereby reabsorbing approximately 80% of the filtered bicarbonate (HCO3(-)), as well as generating "...
Proximal tubule bicarbonate reclamation, conserved biosystem (from KEGG)
Proximal tubule bicarbonate reclamation, conserved biosystemOne of the major tasks of the renal proximal tubule (PT) is to secrete acid into the tubule lumen, thereby reabsorbing approximately 80% of the filtered bicarbonate (HCO3(-)), as well as generating "...
Transmission across Chemical Synapses, organism-specific biosystem (from REACTOME)
Transmission across Chemical Synapses, organism-specific biosystemcomputationally inferred pathway (not manually curated)
citrulline biosynthesis, conserved biosystem (from BIOCYC)
citrulline biosynthesis, conserved biosystemGeneral Background |FRAME: L-CITRULLINE| is a non-standard amino acid that is not normally incorporated into proteins during protein synthesis. The name citrulline was coined in 1930 from Citrullus...
glutamine degradation I, conserved biosystem (from BIOCYC)
glutamine degradation I, conserved biosystemGeneral Background |FRAME: GLN| is the amino group donor for many cellular biosynthetic reactions and serves as a storage reservoir of ammonia, playing an important role in nitrogen metabolism. In...

General gene information

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Homology

Homologs of the Gls2 gene: The Gls2 gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, mouse, zebrafish, and C.elegans.
Map Viewer (Human, Mouse)
OrthoDB: The Hierarchical Catalog of Eukaryotic Orthologs

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
glutaminase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
glutamine metabolic process	IEA Inferred from Electronic Annotation more info
reactive oxygen species metabolic process	IEA Inferred from Electronic Annotation more info
reactive oxygen species metabolic process	ISO Inferred from Sequence Orthology more info
regulation of apoptotic process	IEA Inferred from Electronic Annotation more info
regulation of apoptotic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
mitochondrial matrix	TAS Traceable Author Statement more info	PubMed
mitochondrion	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: glutaminase liver isoform, mitochondrial

Names: glutaminase liver isoform, mitochondrial; GLS; L-glutaminase; L-glutamine amidohydrolase; liver mitochondrial glutaminase

NP_620259.1

EC 3.5.1.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001270786.1 → NP_001257715.1 glutaminase liver isoform, mitochondrial isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (1) encodes the longest isoform (1).

Source sequence(s)

AABR06047168, BC089776

UniProtKB/Swiss-Prot

P28492

Conserved Domains (3) summary

cd00204
Location:490 – 602
Blast Score: 171

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam04960
Location:177 – 463
Blast Score: 1230

Glutaminase; Glutaminase

pfam13637
Location:520 – 572
Blast Score: 133

Ank_4; Ankyrin repeats (many copies)
NM_001270787.1 → NP_001257716.1 glutaminase liver isoform, mitochondrial isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate splice site at the 3' end of the first exon compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1.

Source sequence(s)

AABR06047168, BC089776

UniProtKB/Swiss-Prot

P28492

Conserved Domains (3) summary

cd00204
Location:467 – 579
Blast Score: 172

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam04960
Location:154 – 440
Blast Score: 1232

Glutaminase; Glutaminase

pfam13637
Location:497 – 549
Blast Score: 133

Ank_4; Ankyrin repeats (many copies)
NM_138904.2 → NP_620259.2 glutaminase liver isoform, mitochondrial isoform 3

Status: VALIDATED

Description

Transcript Variant: This variant (3) lacks the first exon and extends 5' into intronic sequence compared to variant 1. The resulting isoform (3) is shorter at the N-terminus compared to isoform 1.

Source sequence(s)

AABR06047168, BC089776

UniProtKB/Swiss-Prot

P28492

Related

ENSRNOP00000018737, ENSRNOT00000018737

Conserved Domains (3) summary

cd00204
Location:423 – 535
Blast Score: 170

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam04960
Location:110 – 396
Blast Score: 1235

Glutaminase; Glutaminase

pfam13637
Location:453 – 505
Blast Score: 132

Ank_4; Ankyrin repeats (many copies)

RefSeqs of Annotated Genomes: Build 5.1

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference Rnor_5.0 Primary Assembly

Genomic

NC_005106.3 Reference Rnor_5.0 Primary Assembly

Range

2589839..2600725

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate Rn_Celera

Genomic

AC_000075.1 Alternate Rn_Celera

Range

501081..511967

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Nucleotide		Protein	Strain
Heading	Accession and Version	Protein	Strain
genomic	AABR06047168.1 (19978..30864)	None
genomic	AAHX01047145.1 (1877..12763)	None
genomic	CH474104.2	EDL84884.1
		EDL84885.1
mRNA	BC089776.1	AAH89776.1
mRNA	BC104712.1	AAI04713.1
mRNA	FQ211735.1	None	Sprague-Dawley
mRNA	J05499.1	AAC37707.1