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    AGL amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase [ Homo sapiens ]

    Gene ID: 178, updated on 12-May-2012

    Summary

    Official Symbol
    AGLprovided by HGNC
    Official Full Name
    amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferaseprovided by HGNC
    Primary source
    HGNC:321
    See related
    Ensembl:ENSG00000162688; HPRD:01984; MIM:610860; Vega:OTTHUMG00000010803
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    GDE
    Summary
    This gene encodes the glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and a amylo-1,6-glucosidase activity. Mutations in this gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing. Alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

    Genomic context

    Location :
    1p21
    Sequence :
    Chromosome: 1; NC_000001.10 (100315640..100389579)

    Chromosome 1 - NC_000001.10Genomic Context describing neighboring genes Neighboring gene ferric-chelate reductase 1 Neighboring gene ribosomal protein L39 pseudogene 9 Neighboring gene uncharacterized LOC730081 Neighboring gene solute carrier family 35 (UDP-N-acetylglucosamine (UDP-GlcNAc) transporter), member A3 Neighboring gene hippocampus abundant transcript 1

    Genomic regions, transcripts, and products

    Bibliography

    GeneRIFs: Gene References Into Functions What's a GeneRIF?

    Phenotypes

    Glycogen storage disease IIIb

    Summary from GeneReviews: Go to GeneReviews

    Disease Characteristics
    Glycogen storage disease type III (GSD III) is characterized by variable liver, cardiac muscle, and skeletal muscle involvement. GSD IIIa, the most common subtype present in about 85% of affected individuals, manifests with liver and muscle involvement; GSD IIIb, with liver involvement only, comprises about 15% of all GSD III. In infancy and early childhood, liver involvement presents as ketotic hypoglycemia, hepatomegaly, hyperlipidemia, and elevated hepatic transaminases. In adolescence and adulthood, liver disease becomes less prominent. Hypertrophic cardiomyopathy develops in the majority of those with GSD IIIa, usually during childhood. Its clinical significance ranges from asymptomatic in the majority to severe cardiac dysfunction, congestive heart failure, and rarely sudden death. Skeletal myopathy manifest as weakness is not usually evident in childhood, but slowly progresses, becoming prominent in the third to fourth decade.
    Diagnosis Testing
    Hepatomegaly, ketotic hypoglycemia with fasting, and elevated serum concentrations of transaminases and CK are the hallmark triad of GSD III. If serum CK is not elevated at the time of diagnostic work-up, absence of lactic acidosis and markedly elevated aspartate aminotransferase (AST) and alanine aminotransferase (ALT) concentrations may provide a clue to diagnosis. Measurement of fasting serum concentration of glucose after glucagon administration can be used to support the diagnosis: the glucose concentration should not rise after a prolonged fast, but should rise after a fast of two hours or less. Molecular genetic testing of AGL, the only gene known to be associated with GSD III, allows confirmation of the diagnosis.
    Genetic Counseling
    GSD III is inherited in an autosomal recessive manner. At conception, each sib of an affected individual has a 25% chance of being affected, a 50% chance of being an asymptomatic carrier, and a 25% chance of being unaffected and not a carrier. Carrier testing for at-risk family members and prenatal testing for pregnancies at increased risk are possible if the disease-causing mutations have been identified in the family.
    References

    Interactions

    Products Interactant Other Gene Complex Source Pubs Description
    P35573 Q9Y478 PRKAB1    HPRD  PubMed  
    BioGRID:106686 BioGRID:132073 NHLRC1    BioGRID  PubMed Affinity Capture-Western 
    BioGRID:106686 BioGRID:111550 PRKAA2    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:106686 BioGRID:111552 PRKAB2    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:106686 BioGRID:123689 RASSF5    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:106686 BioGRID:113164 UBC    BioGRID  PubMed Affinity Capture-MS; Affinity Capture-Western 

    General gene information

    Markers

    Homology

    Pathways from BioSystems

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    4-alpha-glucanotransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    amylo-alpha-1,6-glucosidase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    cation binding IEA
    Inferred from Electronic Annotation
    more info
     
    glycogen debranching enzyme activity TAS
    Traceable Author Statement
    more info
    PubMed 
    hydrolase activity, acting on glycosyl bonds IEA
    Inferred from Electronic Annotation
    more info
     
    polysaccharide binding IEA
    Inferred from Electronic Annotation
    more info
     
    polyubiquitin binding IEA
    Inferred from Electronic Annotation
    more info
     
    protein binding IPI
    Inferred from Physical Interaction
    more info
    PubMed 
    sugar binding IEA
    Inferred from Electronic Annotation
    more info
     
    transferase activity, transferring glycosyl groups IEA
    Inferred from Electronic Annotation
    more info
     
    Process Evidence Code Pubs
    carbohydrate metabolic process TAS
    Traceable Author Statement
    more info
     
    glucose metabolic process TAS
    Traceable Author Statement
    more info
     
    glycogen biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
     
    glycogen catabolic process IBA
    Inferred from Biological aspect of Ancestor
    more info
     
    glycogen catabolic process TAS
    Traceable Author Statement
    more info
     
    response to glucocorticoid stimulus IEA
    Inferred from Electronic Annotation
    more info
     
    response to hormone stimulus IEA
    Inferred from Electronic Annotation
    more info
     
    response to nutrient IEA
    Inferred from Electronic Annotation
    more info
     
    small molecule metabolic process TAS
    Traceable Author Statement
    more info
     
    Component Evidence Code Pubs
    cytoplasm IDA
    Inferred from Direct Assay
    more info
    PubMed 
    cytosol TAS
    Traceable Author Statement
    more info
     
    inclusion body IEA
    Inferred from Electronic Annotation
    more info
     
    isoamylase complex TAS
    Traceable Author Statement
    more info
    PubMed 
    NOT nucleolus IDA
    Inferred from Direct Assay
    more info
    PubMed 
    nucleus IDA
    Inferred from Direct Assay
    more info
    PubMed 
    sarcoplasmic reticulum IEA
    Inferred from Electronic Annotation
    more info
     
    soluble fraction IEA
    Inferred from Electronic Annotation
    more info
     

    General protein information

    Preferred Names
    glycogen debranching enzyme
    Names
    glycogen debranching enzyme
    glycogen debrancher
    amylo-1, 6-glucosidase, 4-alpha-glucanotransferase
    NP_000019.2
    NP_000633.2
    NP_000634.2
    NP_000635.2
    NP_000636.2
    NP_000637.2

    NCBI Reference Sequences (RefSeq)

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    Genomic

    1. NG_012865.1 RefSeqGene

      Range
      5001..78940
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_000028.2NP_000019.2  glycogen debranching enzyme isoform 1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (1). Variants 1, 2, 3 and 4 encode the same isoform (isoform 1).
      Source sequence(s)
      AC096949, U84010
      Consensus CDS
      CCDS759.1
      UniProtKB/Swiss-Prot
      P35573
      Related
      ENSP00000294724, OTTHUMP00000012503, ENST00000294724, OTTHUMT00000029778
      Conserved Domains (3) summary
      TIGR01531
      Location:221532
      Blast Score: 6623
      glyc_debranch; glycogen debranching enzymye
      cd11327
      Location:105583
      Blast Score: 2143
      AmyAc_Glg_debranch_2; Alpha amylase catalytic domain found in glycogen debranching enzymes
      cl01801
      Location:10731527
      Blast Score: 1263
      Bac_rhamnosid; Bacterial alpha-L-rhamnosidase
    2. NM_000642.2NP_000633.2  glycogen debranching enzyme isoform 1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (1) differs in the 5' UTR, compared to variant 4. Variants 1, 2, 3 and 4 encode the same isoform (isoform 1).
      Source sequence(s)
      AC096949, AK130308, BC078663, C00487, U84007
      Consensus CDS
      CCDS759.1
      UniProtKB/Swiss-Prot
      P35573
      Related
      ENSP00000355106, OTTHUMP00000012500, ENST00000361915, OTTHUMT00000029775
      Conserved Domains (3) summary
      TIGR01531
      Location:221532
      Blast Score: 6623
      glyc_debranch; glycogen debranching enzymye
      cd11327
      Location:105583
      Blast Score: 2143
      AmyAc_Glg_debranch_2; Alpha amylase catalytic domain found in glycogen debranching enzymes
      cl01801
      Location:10731527
      Blast Score: 1263
      Bac_rhamnosid; Bacterial alpha-L-rhamnosidase
    3. NM_000643.2NP_000634.2  glycogen debranching enzyme isoform 1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 4. Variants 1, 2, 3 and 4 encode the same isoform (isoform 1).
      Source sequence(s)
      AC096949, U84009
      Consensus CDS
      CCDS759.1
      UniProtKB/Swiss-Prot
      P35573
      Related
      ENSP00000359182, OTTHUMP00000012502, ENST00000370163, OTTHUMT00000029777
      Conserved Domains (3) summary
      TIGR01531
      Location:221532
      Blast Score: 6623
      glyc_debranch; glycogen debranching enzymye
      cd11327
      Location:105583
      Blast Score: 2143
      AmyAc_Glg_debranch_2; Alpha amylase catalytic domain found in glycogen debranching enzymes
      cl01801
      Location:10731527
      Blast Score: 1263
      Bac_rhamnosid; Bacterial alpha-L-rhamnosidase
    4. NM_000644.2NP_000635.2  glycogen debranching enzyme isoform 1

      Status: REVIEWED

      Description
      Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 4. Variants 1, 2, 3 and 4 encode the same isoform (isoform 1).
      Source sequence(s)
      AC096949, U84008
      Consensus CDS
      CCDS759.1
      UniProtKB/Swiss-Prot
      P35573
      Related
      ENSP00000359184, OTTHUMP00000012501, ENST00000370165, OTTHUMT00000029776
      Conserved Domains (3) summary
      TIGR01531
      Location:221532
      Blast Score: 6623
      glyc_debranch; glycogen debranching enzymye
      cd11327
      Location:105583
      Blast Score: 2143
      AmyAc_Glg_debranch_2; Alpha amylase catalytic domain found in glycogen debranching enzymes
      cl01801
      Location:10731527
      Blast Score: 1263
      Bac_rhamnosid; Bacterial alpha-L-rhamnosidase
    5. NM_000645.2NP_000636.2  glycogen debranching enzyme isoform 2

      Status: REVIEWED

      Description
      Transcript Variant: This variant (5) differs in the 5' UTR and coding region, compared to variant 4. The resulting protein (isoform 2) is shorter and has a distinct N-terminus, compared to isoform 1.
      Source sequence(s)
      AC096949, M85168
      Consensus CDS
      CCDS761.1
      UniProtKB/Swiss-Prot
      P35573
      Related
      ENSP00000354635, OTTHUMP00000012505, ENST00000361522, OTTHUMT00000029780
      Conserved Domains (3) summary
      TIGR01531
      Location:111515
      Blast Score: 6598
      glyc_debranch; glycogen debranching enzymye
      cd11327
      Location:88566
      Blast Score: 2144
      AmyAc_Glg_debranch_2; Alpha amylase catalytic domain found in glycogen debranching enzymes
      cl01801
      Location:10561510
      Blast Score: 1262
      Bac_rhamnosid; Bacterial alpha-L-rhamnosidase
    6. NM_000646.2NP_000637.2  glycogen debranching enzyme isoform 3

      Status: REVIEWED

      Description
      Transcript Variant: This variant (6) differs in the 5' UTR and coding region, compared to variant 4. The resulting protein (isoform 3) is shorter and has a distinct N-terminus, compared to isoform 1.
      Source sequence(s)
      AC096949, U84011
      Consensus CDS
      CCDS760.1
      UniProtKB/Swiss-Prot
      P35573
      Related
      ENSP00000354971, OTTHUMP00000012504, ENST00000361302, OTTHUMT00000029779
      Conserved Domains (3) summary
      TIGR01531
      Location:111516
      Blast Score: 6600
      glyc_debranch; glycogen debranching enzymye
      cd11327
      Location:89567
      Blast Score: 2143
      AmyAc_Glg_debranch_2; Alpha amylase catalytic domain found in glycogen debranching enzymes
      cl01801
      Location:10571511
      Blast Score: 1262
      Bac_rhamnosid; Bacterial alpha-L-rhamnosidase

    RefSeqs of Annotated Genomes: Build 37.3

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh37.p5 Primary Assembly

    Genomic

    1. NC_000001.10 Reference GRCh37.p5 Primary Assembly

      Range
      100315640..100389579
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate HuRef

    Genomic

    1. AC_000133.1 Alternate HuRef

      Range
      98438182..98512138
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Related Sequences

    Nucleotide Protein
    Heading Accession and Version
    genomic AB035443.2 BAA88405.1
    genomic AC096949.2 (22928..96868) None
    genomic CH471097.2 EAW72980.1
      EAW72981.1
      EAW72982.1
      EAW72983.1
      EAW72984.1
      EAW72985.1
      EAW72986.1
      EAW72987.1
    mRNA AB208867.1 BAD92104.1
    mRNA AB621804.1 BAK64140.1
    mRNA AK130308.1 None
    mRNA BC020705.1 None
    mRNA BC057220.1 None
    mRNA BC078663.1 AAH78663.1
    mRNA C00487.1 None
    mRNA M85168.1 AAB41040.1
    mRNA U84007.1 AAB48466.1
    mRNA U84008.1 AAB48467.1
    mRNA U84009.1 AAB48468.1
    mRNA U84010.1 AAB48469.1
    mRNA U84011.1 AAB48470.1
    Protein Accession Links
    GenPept Link UniProtKB Link
    P35573.3 GenPept UniProtKB/Swiss-Prot:P35573

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