Klf4 Kruppel-like factor 4 (gut) [Mus musculus (house mouse)] - Gene

Summary

Go to the top of the page Help

Official Symbol: Klf4provided by MGI
Official Full Name: Kruppel-like factor 4 (gut)provided by MGI
Primary source: MGI:1342287
Locus tag: RP23-322L22.2
See related: Ensembl:ENSMUSG00000003032; Vega:OTTMUSG00000007231
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: EZF; Zie; Gklf

Genomic context

Go to the top of the page Help

Location :: 4 B3; 4 29.76 cM

Sequence :: Chromosome: 4; NC_000070.6 (55527137..55532475, complement)

See Klf4 in Epigenomics, MapViewer

Chromosome 4 - NC_000070.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

Go to the top of the page Help

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

Bibliography

Go to the top of the page Help

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Kruppel-like factor 4, a tumor suppressor in hepatocellular carcinoma cells reverts epithelial mesenchymal transition by suppressing slug expression.
Title: Krüppel-like factor 4, a tumor suppressor in hepatocellular carcinoma cells reverts epithelial mesenchymal transition by suppressing slug expression.
show that Zscan4, expressed transiently in2-cell embryos and embryonic stem cells, efficiently produces iPSCs from mouse embryo fibroblasts when coexpressed with Klf4, Oct4, and Sox2
Title: Zscan4 transiently reactivates early embryonic genes during the generation of induced pluripotent stem cells.
CDX2-driven leukemogenesis involves KLF4 repression and deregulated PPARgamma signaling.
Title: CDX2-driven leukemogenesis involves KLF4 repression and deregulated PPARγ signaling.
the HIC-5- and KLF4-dependent mechanism transactivates p21(Cip1) in response to anchorage loss
Title: A HIC-5- and KLF4-dependent mechanism transactivates p21(Cip1) in response to anchorage loss.
KLF4 is an essential regulator of vascular inflammation and experimental atherogenesis.
Title: Myeloid Krüppel-like factor 4 deficiency augments atherogenesis in ApoE-/- mice--brief report.
results suggest precise regulation of KLF4 expression is critical to neuronal differentiation and migration during the formation of a cerebral cortex
Title: Role of Kruppel-like factor 4 in neurogenesis and radial neuronal migration in the developing cerebral cortex.
KLF4 expression in mouse hair bulge stem cells plays an important role in cutaneous wound healing.
Title: Expression of Kruppel-like factor KLF4 in mouse hair follicle stem cells contributes to cutaneous wound healing.
Data show that formation of a Krupple-like factor KLF4, pELK-1, and histone de-acetylase 2 (HDAC2) multiprotein complex dependent on the SM22alpha G/C Repressor element.
Title: Cooperative binding of KLF4, pELK-1, and HDAC2 to a G/C repressor element in the SM22α promoter mediates transcriptional silencing during SMC phenotypic switching in vivo.
KLF4 inhibits cell proliferation, migration and adhesion and loss of KLF4 promotes skin tumorigenesis.
Title: Deficiency of the Kruppel-like factor KLF4 correlates with increased cell proliferation and enhanced skin tumorigenesis.
KLF4 affects intestinal epithelial cell morphology by regulating proliferation, differentiation and polarity of the cells
Title: Krüppel-like factor 4 regulates intestinal epithelial cell morphology and polarity.

Submit: New GeneRIF Correction See all (109)

Alleles

Go to the top of the page Help

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (Floxed/Frt) (1) 1 citation
Targeted (knock-out) (2) 1 citation

Interactions

Go to the top of the page Help

Products	Interactant	Other Gene	Source	Pubs	Description
BioGRID:200966	BioGRID:198143	Apc	BioGRID	PubMed	Phenotypic Enhancement
BioGRID:200966	BioGRID:202313	Pou5f1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:200966	BioGRID:202560	Rad21	BioGRID	PubMed	Co-localization
BioGRID:200966	BioGRID:203336	Smarca4	BioGRID	PubMed	Affinity Capture-Western
BioGRID:200966	BioGRID:204346	Tsix	BioGRID	PubMed	Affinity Capture-RNA

Pathways from BioSystems

Go to the top of the page Help

Developmental Biology, organism-specific biosystem (from REACTOME)
Developmental Biology, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Metabolism of proteins, organism-specific biosystem (from REACTOME)
Metabolism of proteins, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Peptide hormone metabolism, organism-specific biosystem (from REACTOME)
Peptide hormone metabolism, organism-specific biosystemcomputationally inferred pathway (not manually curated)
PluriNetWork, organism-specific biosystem (from WikiPathways)
PluriNetWork, organism-specific biosystemThe aim of the PluriNetWork is to give an overview of specific mechanisms associated with pluripotency in mouse. Each node represents a gene and its corresponding protein product. As stated, we inten...
Synthesis, Secretion, and Deacylation of Ghrelin, organism-specific biosystem (from REACTOME)
Synthesis, Secretion, and Deacylation of Ghrelin, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Transcriptional Regulation of White Adipocyte Differentiation, organism-specific biosystem (from REACTOME)
Transcriptional Regulation of White Adipocyte Differentiation, organism-specific biosystemcomputationally inferred pathway (not manually curated)

General gene information

Go to the top of the page Help

Markers

PMC304100P5 (e-PCR)
- UniSTS:272530

Klf4 (e-PCR), detects polymorphism
- UniSTS:256949

Klf4 (e-PCR), detects polymorphism
- UniSTS:516861

Homology

Homologs of the Klf4 gene: The Klf4 gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, rat, chicken, and zebrafish.
Map Viewer (Human, Rat)
OrthoDB: The Hierarchical Catalog of Eukaryotic Orthologs

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
DNA binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription	ISO Inferred from Sequence Orthology more info
RNA polymerase II transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
RNA polymerase II transcription factor binding	ISO Inferred from Sequence Orthology more info
RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription	ISO Inferred from Sequence Orthology more info
core promoter proximal region sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
double-stranded DNA binding	ISO Inferred from Sequence Orthology more info
metal ion binding	IEA Inferred from Electronic Annotation more info
nucleic acid binding	IEA Inferred from Electronic Annotation more info
phosphatidylinositol 3-kinase regulator activity	ISO Inferred from Sequence Orthology more info
sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
sequence-specific DNA binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
sequence-specific DNA binding transcription factor activity	ISO Inferred from Sequence Orthology more info
sequence-specific DNA binding transcription factor recruiting transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
transcription regulatory region DNA binding	IDA Inferred from Direct Assay more info	PubMed
transcription regulatory region DNA binding	ISO Inferred from Sequence Orthology more info
zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
cellular response to growth factor stimulus	ISO Inferred from Sequence Orthology more info
epidermal cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
epidermis morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
fat cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
negative regulation of ERK1 and ERK2 cascade	ISO Inferred from Sequence Orthology more info
negative regulation of NF-kappaB transcription factor activity	ISO Inferred from Sequence Orthology more info
negative regulation of cell migration involved in sprouting angiogenesis	ISO Inferred from Sequence Orthology more info
negative regulation of cell proliferation	IDA Inferred from Direct Assay more info	PubMed
negative regulation of chemokine (C-X-C motif) ligand 2 production	ISO Inferred from Sequence Orthology more info
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process	ISO Inferred from Sequence Orthology more info
negative regulation of heterotypic cell-cell adhesion	ISO Inferred from Sequence Orthology more info
negative regulation of interleukin-8 biosynthetic process	ISO Inferred from Sequence Orthology more info
negative regulation of muscle hyperplasia	ISO Inferred from Sequence Orthology more info
negative regulation of phosphatidylinositol 3-kinase cascade	ISO Inferred from Sequence Orthology more info
negative regulation of protein kinase B signaling cascade	ISO Inferred from Sequence Orthology more info
negative regulation of response to cytokine stimulus	ISO Inferred from Sequence Orthology more info
negative regulation of smooth muscle cell proliferation	ISO Inferred from Sequence Orthology more info
negative regulation of transcription from RNA polymerase II promoter	ISO Inferred from Sequence Orthology more info
negative regulation of transcription, DNA-dependent	ISO Inferred from Sequence Orthology more info
negative regulation of transcription, DNA-dependent	ISS Inferred from Sequence or Structural Similarity more info	PubMed
negative regulation of transcription, DNA-dependent	NAS Non-traceable Author Statement more info	PubMed
positive regulation of hemoglobin biosynthetic process	ISO Inferred from Sequence Orthology more info
positive regulation of protein metabolic process	ISO Inferred from Sequence Orthology more info
positive regulation of transcription from RNA polymerase II promoter	IDA Inferred from Direct Assay more info	PubMed
positive regulation of transcription from RNA polymerase II promoter	IMP Inferred from Mutant Phenotype more info	PubMed
positive regulation of transcription from RNA polymerase II promoter	ISO Inferred from Sequence Orthology more info
positive regulation of transcription, DNA-dependent	IDA Inferred from Direct Assay more info	PubMed
post-embryonic camera-type eye development	IMP Inferred from Mutant Phenotype more info	PubMed
post-embryonic hemopoiesis	ISO Inferred from Sequence Orthology more info
regulation of cell differentiation	IDA Inferred from Direct Assay more info	PubMed
regulation of cell proliferation	IGI Inferred from Genetic Interaction more info	PubMed
regulation of phosphatidylinositol 3-kinase activity	ISO Inferred from Sequence Orthology more info
regulation of transcription from RNA polymerase II promoter	IDA Inferred from Direct Assay more info	PubMed
regulation of transcription, DNA-dependent	IEA Inferred from Electronic Annotation more info
response to chemical stimulus	IDA Inferred from Direct Assay more info	PubMed
response to retinoic acid	IDA Inferred from Direct Assay more info	PubMed
stem cell maintenance	IDA Inferred from Direct Assay more info	PubMed
transcription from RNA polymerase II promoter	IDA Inferred from Direct Assay more info	PubMed
transcription, DNA-dependent	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
chromatin	ISO Inferred from Sequence Orthology more info
nuclear chromatin	ISO Inferred from Sequence Orthology more info
nucleus	IDA Inferred from Direct Assay more info	PubMed
nucleus	ISO Inferred from Sequence Orthology more info
nucleus	NAS Non-traceable Author Statement more info	PubMed
transcription factor complex	ISO Inferred from Sequence Orthology more info

General protein information

Go to the top of the page Help

Preferred Names: Krueppel-like factor 4

Names: Krueppel-like factor 4; gut-enriched krueppel-like factor; epithelial zinc finger protein EZF; Kruppel-like factor 4 (Gut enriched kruppel-like factor) (Epithelial zinc-finger protein EZF)

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_010637.3 → NP_034767.2 Krueppel-like factor 4

Status: VALIDATED

Source sequence(s)

AA389216, AK141244, AL732494, BQ034021, CF163360

Consensus CDS

CCDS18195.2

UniProtKB/TrEMBL

F2YID5

UniProtKB/Swiss-Prot

Q60793

Related

ENSMUSP00000103245, OTTMUSP00000007677, ENSMUST00000107619, OTTMUST00000016677

Conserved Domains (3) summary

COG5048
Location:395 – 480
Blast Score: 92

COG5048; FOG: Zn-finger [General function prediction only]

pfam13465
Location:446 – 471
Blast Score: 101

zf-H2C2_2; Zinc-finger double domain

cl15478
Location:401 – 424
Blast Score: 76

zf-C2H2; Zinc finger, C2H2 type

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 103

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm38.p1 C57BL/6J

Genomic

NC_000070.6 Reference GRCm38.p1 C57BL/6J

Range

55527137..55532475, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate Mm_Celera

Genomic

AC_000026.1 Alternate Mm_Celera

Range

55439526..55444543, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Nucleotide		Protein	Strain
Heading	Accession and Version	Protein	Strain
genomic	AAHY01036047.1 (12888..17905)	None
genomic	AF117109.1	AAD17223.1
genomic	AL732494.16 (21752..27091)	None
genomic	AY071827.1	AAL60058.1	C57BL/6
genomic	CH466565.2	EDL02262.1	mixed
mRNA	AA389216.1	None	C57BL/6J
mRNA	AK141244.1	BAE24612.1	C57BL/6J
mRNA	AK144942.1	BAE26147.1
mRNA	AK155343.1	BAE33205.1	NOD
mRNA	BC010301.1	AAH10301.2	FVB/N
mRNA	BC091737.1	None	FVB/N
mRNA	BQ034021.1	None	C57BL/6J
mRNA	CF163360.1	None	C57BL/6J
mRNA	JF277566.1	ADZ57169.1	NMRI
mRNA	U20344.1	AAC04892.1
mRNA	U70662.1	AAC52939.1	B6D2F1