DARS aspartyl-tRNA synthetase [Homo sapiens] - Gene

Help top of page

Summary

Official Symbol: DARSprovided by HGNC
Official Full Name: aspartyl-tRNA synthetaseprovided by HGNC
Primary source: HGNC:2678
Locus tag: PIG40
See related: Ensembl:ENSG00000115866; HPRD:04361; MIM:603084; Vega:OTTHUMG00000131741
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: MGC111579; DKFZp781B11202
Summary: Aspartyl-tRNA synthetase (DARS) is part of a multienzyme complex of aminoacyl-tRNA synthetases. Aspartyl-tRNA synthetase charges its cognate tRNA with aspartate during protein biosynthesis. [provided by RefSeq, Jul 2008]

Help top of page

Genomic context

Location :: 2q21.3

Sequence :: Chromosome: 2; NC_000002.11 (136664254..136743222, complement)

See DARS in Epigenomics, MapViewer

Chromosome 2 - NC_000002.11 Genomic Context describing neighboring genes

Help top of page

Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

Help top of page

Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mutations in the nuclear gene coding for the mitochondrial aspartyl-tRNA synthetase, a key enzyme for mitochondrial translation, are correlated with leukoencephalopathy
Title: A human pathology-related mutation prevents import of an aminoacyl-tRNA synthetase into mitochondria.
the N-terminus shows a less-ordered structure with flexible beta-turn, suggesting that when tRNA is stretched, the dissociation rate of Asp-tRNA from aspartyl-tRNA synthetase is reduced, giving time for elongation factor 1alpha to interact with Asp-tRNA.
Title: Structure of the N-terminal extension of human aspartyl-tRNA synthetase: implications for its biological function.

Submit: New GeneRIF Correction

Help top of page

Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

Help top of page

Products	Interactant	Other Gene	Source	Pubs	Description
NP_001340.2		EEF1D	BIND	PubMed	DRS interacts with unspecified isoform of EF-1-delta.
P14868	Q13155	AIMP2	HPRD	PubMed
P14868	P68104	EEF1A1	HPRD	PubMed
BioGRID:107984	BioGRID:113684	AIMP2	BioGRID	PubMed	Two-hybrid
BioGRID:107984	BioGRID:971788	EBNA-LP	BioGRID	PubMed	Affinity Capture-MS
BioGRID:107984	BioGRID:108237	EEF1A1	BioGRID	PubMed	Biochemical Activity
BioGRID:107984	BioGRID:115331	HDAC5	BioGRID	PubMed	Affinity Capture-MS
BioGRID:107984	BioGRID:115669	NDRG1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:107984	BioGRID:114075	PIK3R3	BioGRID	PubMed	Two-hybrid
BioGRID:107984	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS

Function	Evidence Code	Pubs
ATP binding	IEA Inferred from Electronic Annotation more info
aminoacylase activity	TAS Traceable Author Statement more info	PubMed
aspartate-tRNA ligase activity	IEA Inferred from Electronic Annotation more info
ligase activity	IEA Inferred from Electronic Annotation more info
nucleic acid binding	IEA Inferred from Electronic Annotation more info
nucleotide binding	IEA Inferred from Electronic Annotation more info
protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
aspartyl-tRNA aminoacylation	IEA Inferred from Electronic Annotation more info
gene expression	TAS Traceable Author Statement more info
protein complex assembly	TAS Traceable Author Statement more info	PubMed
tRNA aminoacylation for protein translation	TAS Traceable Author Statement more info
translation	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
cytoplasm	IDA Inferred from Direct Assay more info	PubMed
cytosol	TAS Traceable Author Statement more info
soluble fraction	TAS Traceable Author Statement more info	PubMed

General protein information

Preferred Names: aspartate--tRNA ligase, cytoplasmic

Names: aspartate--tRNA ligase, cytoplasmic; aspRS; aspartate tRNA ligase 1, cytoplasmic; aspartyl-tRNA synthetase, cytoplasmic; cell proliferation-inducing protein 40; cell proliferation-inducing gene 40 protein

NP_001340.2

EC 6.1.1.12

Help top of page

NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001349.2 → NP_001340.2 aspartate--tRNA ligase, cytoplasmic

Status: VALIDATED

Source sequence(s)

AK129521, BC000629, BI753718, BU615302

Consensus CDS

CCDS2180.1

UniProtKB/Swiss-Prot

P14868

Related

ENSP00000264161, OTTHUMP00000162534, ENST00000264161, OTTHUMT00000254660

Conserved Domains (3) summary

cd00776
Location:174 – 497
Blast Score: 1137

AsxRS_core; Asx tRNA synthetase (AspRS/AsnRS) class II core domain. Assignment to class II aminoacyl-tRNA synthetases (aaRS) based upon its structure and the presence of three characteristic sequence motifs in the core domain. This family includes AsnRS as well as ...

cd04320
Location:60 – 158
Blast Score: 382

AspRS_cyto_N; AspRS_cyto_N: N-terminal, anticodon recognition domain of the type found in Saccharomyces cerevisiae and human cytoplasmic aspartyl-tRNA synthetase (AspRS). This domain is a beta-barrel domain (OB fold) involved in binding the tRNA anticodon stem-loop. ...

PLN02850
Location:3 – 501
Blast Score: 1792

PLN02850; aspartate-tRNA ligase

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000002.11 Reference GRCh37.p5 Primary Assembly

Range

136664254..136743222, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000134.1 Alternate HuRef

Range

128656738..128735990, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Help top of page

Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AC011999.9	AAY15029.1
genomic	AC093391.5	AAY24055.1
genomic	CH471058.2	EAX11617.1
		EAX11618.1
		EAX11619.1
		EAX11620.1
mRNA	AK129521.1	None
mRNA	AK222476.1	BAD96196.1
mRNA	AK290607.1	BAF83296.1
mRNA	AY762100.1	AAX07827.1
mRNA	BC000629.2	AAH00629.1
mRNA	BC107749.1	AAI07750.1
mRNA	BI753718.1	None
mRNA	BT006710.1	AAP35356.1
mRNA	BU615302.1	None
mRNA	CR749809.1	CAH18669.1
mRNA	J05032.1	AAA35567.1
other-genetic	DQ890741.2	ABM81667.1
other-genetic	DQ893912.2	ABM84838.1

Protein Accession	Links
Protein Accession	GenPept Link	UniProtKB Link
P14868.2	GenPept	UniProtKB/Swiss-Prot:P14868
Q53R85	GenPept	UniProtKB/TrEMBL:Q53R85
Q53T60	GenPept	UniProtKB/TrEMBL:Q53T60
Q68CR9	GenPept	UniProtKB/TrEMBL:Q68CR9