AMOT angiomotin [Homo sapiens] - Gene

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Summary

Official Symbol: AMOTprovided by HGNC
Official Full Name: angiomotinprovided by HGNC
Primary source: HGNC:17810
See related: Ensembl:ENSG00000126016; HPRD:02327; MIM:300410; Vega:OTTHUMG00000022216
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: KIAA1071
Summary: This gene belongs to the motin family of angiostatin binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

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Genomic context

Location :: Xq23

Sequence :: Chromosome: X; NC_000023.10 (112018105..112084043, complement)

See AMOT in Epigenomics, MapViewer

Chromosome X - NC_000023.10 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that Amot expression is required for proliferation of breast cancer cells in specific microenvironmental contexts that require ERK1/2 signaling.
Title: The adaptor protein AMOT promotes the proliferation of mammary epithelial cells via the prolonged activation of the extracellular signal-regulated kinases.
ADepletion of Angiomotin in Nf2(-/-) Schwann cells attenuates the Ras-MAPK signaling pathway, impedes cellular proliferation in vitro and tumorigenesis in vivo
Title: A tight junction-associated Merlin-angiomotin complex mediates Merlin's regulation of mitogenic signaling and tumor suppressive functions.
novel mechanism to restrict the activity of TAZ and YAP through physical interaction with Amot and AmotL1
Title: Hippo pathway-independent restriction of TAZ and YAP by angiomotin.
Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.
a novel lipid binding domain within Amot is shown to selectively bind with high affinity to membranes containing monophosphorylated phosphatidylinositols and cholesterol
Title: Amot recognizes a juxtanuclear endocytic recycling compartment via a novel lipid binding domain.
The angiomotin-like 1 is involved in actin-cytoskeleton-based processes, in part, via its interaction with a p80-angiomotin-containing complex and the actin cytoskeleton
Title: Human angiomotin-like 1 associates with an angiomotin protein complex through its coiled-coil domain and induces the remodeling of the actin cytoskeleton.
Amot and AmotL1 have similar effects on endothelial migration and tight junction formation in vitro. In vivo Amot appears to control the cell polarity and AmotL1 affects the stability of cell-cell junctions.
Title: Angiomotin-like protein 1 controls endothelial polarity and junction stability during sprouting angiogenesis.
the directional migration of capillaries in the embryo is governed by the Amot:Patj/Mupp1:Syx signaling that controls local GTPase activity
Title: The Amot/Patj/Syx signaling complex spatially controls RhoA GTPase activity in migrating endothelial cells.
Results show that DNA vaccination targeting angiomotin may be used to mimic the effect of angiostatin.
Title: A DNA vaccine targeting angiomotin inhibits angiogenesis and suppresses tumor growth.

Submit: New GeneRIF Correction See all (13)

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Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

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Products	Interactant	Other Gene	Source	Pubs	Description
Q4VCS5	Q96QZ7	MAGI1	HPRD	PubMed
Q4VCS5	P00747	PLG	HPRD	PubMed
BioGRID:127557	BioGRID:127559	AMOTL1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:127557	BioGRID:114907	BAG3	BioGRID	PubMed	Affinity Capture-MS
BioGRID:127557	BioGRID:108083	DLG1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:127557	BioGRID:114206	DYNLL1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:127557	BioGRID:126680	DYNLL2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:127557	BioGRID:128769	FOXK1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:127557	BioGRID:109820	FOXK2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:127557	BioGRID:114352	LIN7A	BioGRID	PubMed	Affinity Capture-MS
BioGRID:127557	BioGRID:120608	LIN7C	BioGRID	PubMed	Affinity Capture-MS
BioGRID:127557	BioGRID:123755	LONP2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:127557	BioGRID:110438	MLH1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:127557	BioGRID:114307	MPDZ	BioGRID	PubMed	Affinity Capture-MS
BioGRID:127557	BioGRID:122155	MPP5	BioGRID	PubMed	Affinity Capture-MS
BioGRID:127557	BioGRID:126785	MPP7	BioGRID	PubMed	Affinity Capture-MS
BioGRID:127557	BioGRID:116915	NEDD4L	BioGRID	PubMed	Affinity Capture-MS
BioGRID:127557	BioGRID:110844	NF2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:127557	BioGRID:119372	NIN	BioGRID	PubMed	Affinity Capture-MS
BioGRID:127557	BioGRID:111550	PRKAA2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:127557	BioGRID:126318	SCLT1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:127557	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS
BioGRID:127557	BioGRID:119697	UIMC1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:127557	BioGRID:113866	USP11	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western
BioGRID:127557	BioGRID:123904	USP42	BioGRID	PubMed	Affinity Capture-MS
BioGRID:127557	BioGRID:115684	YAP1	BioGRID	PubMed	Affinity Capture-MS

Function	Evidence Code	Pubs
angiostatin binding	IDA Inferred from Direct Assay more info	PubMed
protein binding	IPI Inferred from Physical Interaction more info	PubMed
receptor activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
actin cytoskeleton organization	TAS Traceable Author Statement more info	PubMed
cell migration involved in gastrulation	IEA Inferred from Electronic Annotation more info
cell-cell junction assembly	TAS Traceable Author Statement more info	PubMed
cellular protein localization	IDA Inferred from Direct Assay more info
chemotaxis	IEA Inferred from Electronic Annotation more info
gastrulation with mouth forming second	IEA Inferred from Electronic Annotation more info
hippo signaling cascade	IGI Inferred from Genetic Interaction more info
in utero embryonic development	IEA Inferred from Electronic Annotation more info
negative regulation of angiogenesis	IDA Inferred from Direct Assay more info	PubMed
negative regulation of vascular permeability	IDA Inferred from Direct Assay more info	PubMed
positive regulation of blood vessel endothelial cell migration	IDA Inferred from Direct Assay more info	PubMed
positive regulation of cell size	TAS Traceable Author Statement more info	PubMed
positive regulation of embryonic development	IEA Inferred from Electronic Annotation more info
positive regulation of stress fiber assembly	TAS Traceable Author Statement more info	PubMed
regulation of cell migration	IDA Inferred from Direct Assay more info	PubMed
vasculogenesis	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
actin filament	IDA Inferred from Direct Assay more info	PubMed
cell junction	IEA Inferred from Electronic Annotation more info
cell surface	IDA Inferred from Direct Assay more info	PubMed
cytoplasm	IDA Inferred from Direct Assay more info	PubMed
endocytic vesicle	IDA Inferred from Direct Assay more info	PubMed
external side of plasma membrane	IDA Inferred from Direct Assay more info	PubMed
integral to membrane	IDA Inferred from Direct Assay more info	PubMed
lamellipodium	IDA Inferred from Direct Assay more info	PubMed
ruffle	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with signalosome	IDA Inferred from Direct Assay more info	PubMed
stress fiber	IDA Inferred from Direct Assay more info	PubMed
tight junction	IDA Inferred from Direct Assay more info	PubMed
tight junction	IEA Inferred from Electronic Annotation more info

General protein information

Preferred Names: angiomotin

Names: angiomotin; angiomotin p80 isoform; angiomotin p130 isoform

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NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_012628.1 RefSeqGene

Range

5001..70939

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001113490.1 → NP_001106962.1 angiomotin isoform 1

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). The N-terminus of this isoform mediates association with cytoplasmic F-actin.

Source sequence(s)

AB028994, AF286598, AY987378

Consensus CDS

CCDS48154.1

UniProtKB/Swiss-Prot

Q4VCS5

Related

ENSP00000361027, ENST00000371959

Conserved Domains (2) summary

pfam12240
Location:599 – 794
Blast Score: 586

Angiomotin_C; Angiomotin C terminal

PRK03918
Location:423 – 540
Blast Score: 94

PRK03918; chromosome segregation protein; Provisional
NM_133265.2 → NP_573572.1 angiomotin isoform 2

Status: REVIEWED

Description

Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. This difference results in translation initiation at a downstream ATG and an isoform (2) with a shorter N-terminus, compared to isoform 1. This isoform lacks the N-terminal extension present in isoform 1 that mediates association with cytoplasmic F-actin.

Source sequence(s)

AB028994, AF286598

Consensus CDS

CCDS14563.1

UniProtKB/Swiss-Prot

Q4VCS5

Related

ENSP00000305557, OTTHUMP00000023866, ENST00000304758, OTTHUMT00000057950

Conserved Domains (1) summary

pfam12240
Location:190 – 385
Blast Score: 572

Angiomotin_C; Angiomotin C terminal

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000023.10 Reference GRCh37.p5 Primary Assembly

Range

112018105..112084043, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000155.1 Alternate HuRef

Range

101645090..101710229, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

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Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AC004827.1 (10576..76514)	None
genomic	CH471120.2	EAX02626.1
		EAX02627.1
mRNA	AB028994.3	BAA83023.3
mRNA	AF286598.1	AAG01851.1
mRNA	AK024344.1	None
mRNA	AK301573.1	BAG63066.1
mRNA	AY987378.1	AAY24451.1
mRNA	BC039408.1	None
mRNA	BC050581.1	None
mRNA	BC094712.1	AAH94712.1
mRNA	BC130294.1	AAI30295.1