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    SV40gp6 large T antigen [ Simian virus 40 ]

    Gene ID: 1489531, updated on 4-Feb-2012
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    Summary

    Gene symbol
    SV40gp6
    Gene description
    large T antigen
    Locus tag
    SV40gp6
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Simian virus 40 (old-name: Polyomavirus maccacae)
    Lineage
    Viruses; dsDNA viruses, no RNA stage; Polyomaviridae; Polyomavirus
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    Genomic context

    Sequence :
    NC_001669.1 (2691..5163, complement)

    NC_001669.1Genomic Context describing neighboring genes Neighboring gene agnoprotein (lp-1, VCP) Neighboring gene VP2 minor structural protein Neighboring gene VP3 minor structural protein Neighboring gene VP1 major structural protein Neighboring gene miscRNA Neighboring gene small t antigen Neighboring gene early leader protein (SELP; [133])

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    Genomic regions, transcripts, and products

    Go to nucleotideGraphics FASTA GenBank

    Go to reference sequence details
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    Bibliography

    Related articles in PubMed

    1. Structure-based design of a disulfide-linked oligomeric form of the simian virus 40 (SV40) large T antigen DNA-binding domain. Meinke G, et al. Acta Crystallogr D Biol Crystallogr, 2011 Jun. PMID 21636896.
    2. Structure-based analysis of the interaction between the simian virus 40 T-antigen origin binding domain and single-stranded DNA. Meinke G, et al. J Virol, 2011 Jan. PMID 20980496.
    3. Multiple DNA damage signaling and repair pathways deregulated by simian virus 40 large T antigen. Boichuk S, et al. J Virol, 2010 Aug. PMID 20519379.
    4. Conformational rearrangements of SV40 large T antigen during early replication events. Cuesta I, et al. J Mol Biol, 2010 Apr 16. PMID 20219473.
    5. The SV40 large T-antigen origin binding domain directly participates in DNA unwinding. Foster EC, et al. Biochemistry, 2010 Mar 16. PMID 20108984.

    See all (78) citations in PubMed

    GeneRIFs: Gene References Into Functions What's a GeneRIF?

    1. mutant large T antigen origin-binding domain formed higher oligomeric products in a redox-dependent manner
      Title: Structure-based design of a disulfide-linked oligomeric form of the simian virus 40 (SV40) large T antigen DNA-binding domain.
    2. These studies refine the understanding of how the large T antigen origin-binding domain s encoded by the polyomavirus family interact with single-stranded DNA, a critical step during the initiation of DNA replication.
      Title: Structure-based analysis of the interaction between the simian virus 40 T-antigen origin binding domain and single-stranded DNA.
    3. Taken together, the interplay of large T antigen with the DNA damage response is more complex than anticipated, with individual domains of large T antigen being connected to different subcomponents of the DNA damage response and repair machinery.
      Title: Multiple DNA damage signaling and repair pathways deregulated by simian virus 40 large T antigen.
    4. used three-dimensional cryo-electron microscopy to visualize early events in the activation of DNA replication in the SV40; the assembly of an LTag double hexamer at the origin DNA that will subsequently melt the origin DNA to initiate fork unwinding
      Title: Conformational rearrangements of SV40 large T antigen during early replication events.
    5. a model of DNA threading through the large T-antigen complex illustrating how single-stranded DNA could pass close to a trough generated by key residues in one monomer of the origin binding domain hexamer
      Title: The SV40 large T-antigen origin binding domain directly participates in DNA unwinding.
    6. Simian virus 40 large T antigen interacting with TACC2 is involved in stabilizing microtubules in mitosis.
      Title: Simian virus 40 large T antigen targets the microtubule-stabilizing protein TACC2.
    7. practically all gene expression regulated by TAg in enterocytes is dependent upon its binding and inactivation of the Rb family proteins
      Title: Simian virus 40 T-antigen-mediated gene regulation in enterocytes is controlled primarily by the Rb-E2F pathway.
    8. These results suggest that the histone acetyltransferase CBP/p300 is one of the potential targets affected by the mutations in large T antigen.
      Title: A structure-guided mutational analysis of simian virus 40 large T antigen: identification of surface residues required for viral replication and transformation.
    9. SV40 transformation alters the expression of approximately 800 cellular genes in both systems. Much of this regulation is observed in both MEFs and enterocytes and is consistent with T antigen action on the Rb-E2F pathway.
      Title: Cell-type specific regulation of gene expression by simian virus 40 T antigens.
    10. These data indicate that T antigen has a mechanism to specifically unwind the central palindrome in the replication origin DNA.
      Title: Simian virus 40 large T antigen can specifically unwind the central palindrome at the origin of DNA replication.
    Submit: New GeneRIF Correction See all (27)
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    Interactions

    Products Interactant Other Gene Complex Source Pubs Description
    NP_043127.1 NP_002257.1 KPNA2    BIND  PubMed TAg interacts with karyopherin alpha (K-alpha). 
    NP_043127.1 NP_002257.1 KPNA2    BIND  PubMed SV40T interacts with Imp-alpha. 
    NP_043127.1 NP_034785.1 Kpna2    BIND  PubMed Importin-alpha-2 interacts with T-Ag. 
    NP_043127.1 NP_000312.1 RB1    BIND  PubMed pRB interacts with SV40 T antigen. 
    NP_043127.1 NP_002936.1 RPA1    BIND  PubMed TAg interacts with p70. 
    NP_043127.1 NP_000537.2 TP53    BIND  PubMed p53 interacts with SV40 large T antigen. This interaction was modelled on a demonstrated interaction between p53 from an unsepcified species and SV40 large T antigen. 
    NP_043127.1 NP_000537.2 TP53    BIND  PubMed p53 interacts with SV40 large T antigen. This interaction was modelled on a demonstrated interaction between p53 from an unspecified species and Simian virus 40 large T antigen. 
    NP_043127.1 AAA37085.1     BIND  PubMed TP53 (p53) interacts with large T antigen. 
    NP_043127.1     BIND  PubMed T-Ag interacts with Importin-alpha-2-Importin-beta-1. 
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    General protein information

    Names
    large T antigen
    YP_003708382.1
    • large T antigen; binds to the viral origin of replication; regulates the transcription of early region mRNA; helicase activity facilitates movement of the virion DNA replication fork; the small T antigen shares a region in common with the large T antigen known as the J-domain; the J-domain is required for multiple processes including assembly, viral genomic DNA replication and transcription; The polyomaviruses are non-enveloped dsDNA viruses; the viral genome is organized into two divergent transcriptional units known as early and late regions; mRNAs from the early region encode the viral small and large t-antigens; the small T antigen and large T antigen are expressed upon entry into the host nucleus early in the viral lifecycle
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    NCBI Reference Sequences (RefSeq)

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001669.1

      Range
      2691..5163, complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. YP_003708382.1 large T antigen [Simian virus 40]

      UniProtKB/Swiss-Prot
      P03070
      UniProtKB/TrEMBL
      Q9DH70
      Conserved Domains (4) summary
      pfam02217
      Location:136 – 229
      Blast Score: 412
      T_Ag_DNA_bind; Origin of replication binding protein
      pfam06431
      Location:265 – 683
      Blast Score: 2066
      Polyoma_lg_T_C; Polyomavirus large T antigen C-terminus
      PHA02624
      Location:1 – 630
      Blast Score: 2911
      PHA02624; large T antigen; Provisional
      cl02542
      Location:13 – 67
      Blast Score: 136
      DnaJ; DnaJ domain or J-domain. DnaJ/Hsp40 (heat shock protein 40) proteins are highly conserved and play crucial roles in protein translation, folding, unfolding, translocation, and degradation. They act primarily by stimulating the ATPase activity of Hsp70s, ...
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    Related Sequences

    Nucleotide Protein
    Heading Accession and Version
    genomic EF579667.1 ABU86097.1
    genomic AF316141.1 AAG39214.1
    genomic EF579804.1 ABU62650.1
    genomic J02400.1 AAB59924.1
    genomic AF316139.1 AAG39202.1
    genomic EF579663.1 ABU86073.1
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q9DH70.1 GenPept
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    Additional Links

    Gene LinkOut

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