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    CSTF2 cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa [ Homo sapiens ]

    Gene ID: 1478, updated on 11-May-2012
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    Summary

    Official Symbol
    CSTF2provided by HGNC
    Official Full Name
    cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDaprovided by HGNC
    Primary source
    HGNC:2484
    Locus tag
    RP1-146H21.2
    See related
    Ensembl:ENSG00000101811; HPRD:02652; MIM:600368; Vega:OTTHUMG00000022709
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    CstF-64
    Summary
    This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs. [provided by RefSeq, Jul 2008]
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    Genomic context

    Location :
    Xq22.1
    Sequence :
    Chromosome: X; NC_000023.10 (100075348..100095924)
    See CSTF2 in Epigenomics, MapViewer

    Chromosome X - NC_000023.10Genomic Context describing neighboring genes Neighboring gene peptidylprolyl isomerase A (cyclophilin A) pseudogene Neighboring gene RAD21 homolog (S. pombe) pseudogene Neighboring gene NADPH oxidase 1 Neighboring gene heterogeneous nuclear ribonucleoprotein A1 pseudogene 26 Neighboring gene heterogeneous nuclear ribonucleoprotein A1 pseudogene 27

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    Genomic regions, transcripts, and products

    Go to nucleotideGraphics FASTA GenBank

    Go to reference sequence details
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    Bibliography

    Related articles in PubMed

    1. Systematic and quantitative assessment of the ubiquitin-modified proteome. Kim W, et al. Mol Cell, 2011 Oct 21. PMID 21906983.
    2. A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles. Wagner SA, et al. Mol Cell Proteomics, 2011 Oct. PMID 21890473.
    3. Characterization of a cleavage stimulation factor, 3' pre-RNA, subunit 2, 64 kDa (CSTF2) as a therapeutic target for lung cancer. Aragaki M, et al. Clin Cancer Res, 2011 Sep 15. PMID 21813631.
    4. Interactions of CstF-64, CstF-77, and symplekin: implications on localisation and function. Ruepp MD, et al. Mol Biol Cell, 2011 Jan 1. PMID 21119002.
    5. A data set of human endogenous protein ubiquitination sites. Shi Y, et al. Mol Cell Proteomics, 2011 May. PMID 20972266.

    See all (43) citations in PubMed

    See citations in PubMed for homologs of this gene provided by HomoloGene

    GeneRIFs: Gene References Into Functions What's a GeneRIF?

    1. CSTF2 is likely to play an important role in lung carcinogenesis and be a prognostic biomarker in the clinic.
      Title: Characterization of a cleavage stimulation factor, 3' pre-RNA, subunit 2, 64 kDa (CSTF2) as a therapeutic target for lung cancer.
    2. nuclear accumulation of CstF-64 depends on binding to CstF-77 not symplekin; interaction between CstF-64/CstF-64Tau and CstF-77 are important for maintenance of nuclear levels of CstF complex components and intracellular localization, stability, function
      Title: Interactions of CstF-64, CstF-77, and symplekin: implications on localisation and function.
    3. The Hinge domain is necessary for CstF-64 interaction with CstF-77 and consequent nuclear localization.
      Title: The hinge domain of the cleavage stimulation factor protein CstF-64 is essential for CstF-77 interaction, nuclear localization, and polyadenylation.
    4. CstF64 (cstf2) and not other CstF subunits induced by lipopolysaccharide (LPS) in murine macrophages and changes polyA site use
      Title: Elevated levels of the 64-kDa cleavage stimulatory factor (CstF-64) in lipopolysaccharide-stimulated macrophages influence gene expression and induce alternative poly(A) site selection.
    5. The inactivity of the RSV poly(A) site was at least in part due to poor CstF binding since tethering CstF to the RSV substrate activated polyadenylation.
      Title: Characterization of Rous sarcoma virus polyadenylation site use in vitro.
    6. dynamics of the CstF-64 RNA-binding domain, both free and bound to two GU-rich RNA sequences that represent polyadenylation regulatory elements, by NMR Spectroscopy
      Title: Protein and RNA dynamics play key roles in determining the specific recognition of GU-rich polyadenylation regulatory elements by human Cstf-64 protein.
    7. Describes the induction of CSTF2 in the growth phase of cells.
      Title: Increase in the 64-kDa subunit of the polyadenylation/cleavage stimulatory factor during the G0 to S phase transition.
    Submit: New GeneRIF Correction
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    Phenotypes

    Review eQTL and phenotype association data in this region using PheGenI

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    Interactions

    Products Interactant Other Gene Complex Source Pubs Description
    NP_001316.1 NP_006704.2 SUB1    BIND  PubMed PC4 interacts with CSTF2 (CstF-64). 
    P33240 P46379 BAG6    HPRD  PubMed  
    P33240 Q9UKR5 C14orf1    HPRD  PubMed  
    P33240 Q12873 CHD3    HPRD  PubMed  
    P33240 Q12996 CSTF3    HPRD  PubMed  
    P33240 Q92499 DDX1    HPRD  PubMed  
    P33240 Q13011 ECH1    HPRD  PubMed  
    P33240 P26641 EEF1G    HPRD  PubMed  
    P33240 Q99689 FEZ1    HPRD  PubMed  
    P33240 O95163 IKBKAP    HPRD  PubMed  
    P33240 Q16891 IMMT    HPRD  PubMed  
    P33240 Q92993 KAT5    HPRD  PubMed  
    P33240 Q9P2H0 KIAA1377    HPRD  PubMed  
    P33240 P53999 SUB1    HPRD  PubMed  
    P33240 Q92797 SYMPK    HPRD  PubMed  
    P33240 Q9UMX0 UBQLN1    HPRD  PubMed  
    P33240 Q9NRR5 UBQLN4    HPRD  PubMed  
    BioGRID:107860 BioGRID:113647 BAG6    BioGRID  PubMed Two-hybrid 
    BioGRID:107860 BioGRID:107056 BARD1    BioGRID  PubMed Affinity Capture-Western; Co-purification 
    BioGRID:107860 BioGRID:107140 BRCA1    BioGRID  PubMed Affinity Capture-Western; Co-purification 
    BioGRID:107860 BioGRID:116332 C14orf1    BioGRID  PubMed Two-hybrid 
    BioGRID:107860 BioGRID:122724 CDC73    BioGRID  PubMed Affinity Capture-MS; Affinity Capture-Western 
    BioGRID:107860 BioGRID:107532 CHD3    BioGRID  PubMed Two-hybrid 
    BioGRID:107860 BioGRID:118946 CPSF1    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:107860 BioGRID:119826 CPSF2    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:107860 BioGRID:107859 CSTF1    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:107860 BioGRID:107861 CSTF3    BioGRID  PubMed Affinity Capture-MS; Reconstituted Complex 
    BioGRID:107860 BioGRID:108019 DDX1    BioGRID  PubMed Affinity Capture-Western 
    BioGRID:107860 BioGRID:108220 ECH1    BioGRID  PubMed Two-hybrid 
    BioGRID:107860 BioGRID:108257 EEF1G    BioGRID  PubMed Two-hybrid 
    BioGRID:107860 BioGRID:114997 FEZ1    BioGRID  PubMed Two-hybrid 
    BioGRID:107860 BioGRID:114090 IKBKAP    BioGRID  PubMed Two-hybrid 
    BioGRID:107860 BioGRID:116185 IMMT    BioGRID  PubMed Two-hybrid 
    BioGRID:107860 BioGRID:115779 KAT5    BioGRID  PubMed Two-hybrid 
    BioGRID:107860 BioGRID:121617 KIAA1377    BioGRID  PubMed Two-hybrid 
    BioGRID:107860 BioGRID:115544 SRRM1    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:107860 BioGRID:116127 SUB1    BioGRID  PubMed Affinity Capture-Western; Reconstituted Complex; Two-hybrid 
    BioGRID:107860 BioGRID:113833 SYMPK    BioGRID  PubMed Affinity Capture-MS; Reconstituted Complex 
    BioGRID:107860 BioGRID:113164 UBC    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:107860 BioGRID:119007 UBQLN1    BioGRID  PubMed Two-hybrid 
    BioGRID:107860 BioGRID:971443 UL48    BioGRID  PubMed Affinity Capture-Western 
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    General gene information

    Markers

    Genotypes

    Homology

    Pathways from BioSystems

    • Cleavage of Growing Transcript in the Termination Region, organism-specific biosystem (from REACTOME)
      Cleavage of Growing Transcript in the Termination Region, organism-specific biosystemThis section includes the cleavage of both polyadenylated and non-polyadenylated transcripts. In the former case polyadenylation has to precede transcript cleavage, while in the latter case there is ...
    • Gene Expression, organism-specific biosystem (from REACTOME)
      Gene Expression, organism-specific biosystemGene Expression covers the pathways by which genomic DNA is transcribed to yield RNA, the regulation of these transcription processes, and the pathways by which newly-made RNA Transcripts are process...
    • Processing of Capped Intron-Containing Pre-mRNA, organism-specific biosystem (from REACTOME)
      Processing of Capped Intron-Containing Pre-mRNA, organism-specific biosystemCo-transcriptional pre-mRNA splicing is not obligatory. Pre-mRNA splicing begins co-transcriptionally and often continues post-transcriptionally. Human genes contain an average of nine introns per ge...
    • Processing of Capped Intronless Pre-mRNA, organism-specific biosystem (from REACTOME)
      Processing of Capped Intronless Pre-mRNA, organism-specific biosystemCo-transcriptional pre-mRNA splicing is not obligatory. Pre-mRNA splicing begins co-transcriptionally and often continues post-transcriptionally. Human genes contain an average of nine introns per ge...
    • Processing of Intronless Pre-mRNAs, organism-specific biosystem (from REACTOME)
      Processing of Intronless Pre-mRNAs, organism-specific biosystemThe 3' ends of eukaryotic mRNAs are generated by posttranscriptional processing of an extended primary transcript. For almost all RNAs, 3' processing consists of two steps: The mRNA is first cleaved ...
    • RNA Polymerase II Transcription, organism-specific biosystem (from REACTOME)
      RNA Polymerase II Transcription, organism-specific biosystem
      RNA Polymerase II Transcription
    • RNA Polymerase II Transcription Termination, organism-specific biosystem (from REACTOME)
      RNA Polymerase II Transcription Termination, organism-specific biosystemThe detailed annotation of this section will be completed in the next release.
    • mRNA 3'-end processing, organism-specific biosystem (from REACTOME)
      mRNA 3'-end processing, organism-specific biosystemThe 3' ends of eukaryotic mRNAs are generated by posttranscriptional processing of an extended primary transcript. For almost all RNAs, 3'-end processing consists of two steps: (i) the mRNA is first ...
    • mRNA Splicing, organism-specific biosystem (from REACTOME)
      mRNA Splicing, organism-specific biosystemThe process in which excision of introns from the primary transcript of messenger RNA (mRNA) is followed by ligation of the two exon termini exposed by removal of each intron, is called mRNA splicing...
    • mRNA Splicing - Major Pathway, organism-specific biosystem (from REACTOME)
      mRNA Splicing - Major Pathway, organism-specific biosystemThe splicing of pre-mRNA occurs within a large, very dynamic complex, designated the 'spliceosome'. The 50-60S spliceosomes are estimated to be 40-60 nm in diameter, and have molecular weights in the...
    • mRNA processing, organism-specific biosystem (from WikiPathways)
      mRNA processing, organism-specific biosystemThis process describes the conversion of precursor messenger RNA into mature messenger RNA (mRNA). The pre-mRNA molecule undergoes three main modifications. These modifications are 5' capping, 3' po...
    • mRNA surveillance pathway, organism-specific biosystem (from KEGG)
      mRNA surveillance pathway, organism-specific biosystemThe mRNA surveillance pathway is a quality control mechanism that detects and degrades abnormal mRNAs. These pathways include nonsense-mediated mRNA decay (NMD), nonstop mRNA decay (NSD), and no-go ...
    • mRNA surveillance pathway, conserved biosystem (from KEGG)
      mRNA surveillance pathway, conserved biosystemThe mRNA surveillance pathway is a quality control mechanism that detects and degrades abnormal mRNAs. These pathways include nonsense-mediated mRNA decay (NMD), nonstop mRNA decay (NSD), and no-go ...

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    RNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    nucleotide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    RNA splicing TAS
    Traceable Author Statement
    more info
    		  
    gene expression TAS
    Traceable Author Statement
    more info
    		  
    mRNA 3'-end processing TAS
    Traceable Author Statement
    more info
    		  
    mRNA cleavage TAS
    Traceable Author Statement
    more info
    		 PubMed 
    mRNA polyadenylation TAS
    Traceable Author Statement
    more info
    		 PubMed 
    mRNA processing TAS
    Traceable Author Statement
    more info
    		  
    nuclear mRNA splicing, via spliceosome TAS
    Traceable Author Statement
    more info
    		  
    termination of RNA polymerase II transcription TAS
    Traceable Author Statement
    more info
    		  
    transcription from RNA polymerase II promoter TAS
    Traceable Author Statement
    more info
    		  
    Component Evidence Code Pubs
    cleavage body IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    mRNA cleavage and polyadenylation specificity factor complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    NOT nucleolus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    nucleoplasm TAS
    Traceable Author Statement
    more info
    		  
    nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
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    General protein information

    Preferred Names
    cleavage stimulation factor subunit 2
    Names
    cleavage stimulation factor subunit 2
    CF-1 64 kDa subunit
    CSTF 64 kDa subunit
    betaCstF-64 variant 2
    cleavage stimulation factor 64 kDa subunit
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    NCBI Reference Sequences (RefSeq)

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    Genomic

    1. NG_015848.1 RefSeqGene

      Range
      5001..25577
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001325.2 → NP_001316.1  cleavage stimulation factor subunit 2

      Status: REVIEWED

      Source sequence(s)
      AW016125, M85085, Z83819, Z95327
      Consensus CDS
      CCDS14473.1
      UniProtKB/Swiss-Prot
      P33240
      Related
      ENSP00000362063, OTTHUMP00000024310, ENST00000372972, OTTHUMT00000058926
      Conserved Domains (3) summary
      cd00590
      Location:17 – 90
      Blast Score: 260
      RRM; RRM (RNA recognition motif), also known as RBD (RNA binding domain) or RNP (ribonucleoprotein domain), is a highly abundant domain in eukaryotes found in proteins involved in post-transcriptional gene expression processes including mRNA and rRNA ...
      pfam14304
      Location:531 – 574
      Blast Score: 151
      CSTF_C; Transcription termination and cleavage factor C-terminal
      pfam14327
      Location:109 – 193
      Blast Score: 277
      CSTF2_hinge; Hinge domain of cleavage stimulation factor subunit 2

    RefSeqs of Annotated Genomes: Build 37.3

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh37.p5 Primary Assembly

    Genomic

    1. NC_000023.10 Reference GRCh37.p5 Primary Assembly

      Range
      100075348..100095924
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate HuRef

    Genomic

    1. AC_000155.1 Alternate HuRef

      Range
      89874108..89894686
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)
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    Related Sequences

    Nucleotide Protein
    Heading Accession and Version
    genomic CH471115.1 EAX02818.1
      EAX02819.1
    genomic Z83819.1 CAB06072.2
    genomic Z95327.1 CAI42680.1
      CAI42681.1
    mRNA AK095684.1 None
    mRNA AK300600.1 BAG62297.1
    mRNA AW016125.1 None
    mRNA BC017712.1 AAH17712.1
    mRNA BC033135.1 AAH33135.1
    mRNA BT009778.1 AAP88780.1
    mRNA EU616679.1 ACF05699.1
    mRNA M85085.1 AAA35724.1
    other-genetic DQ891532.2 ABM82458.1
    other-genetic DQ894721.2 ABM85647.1
    Protein Accession Links
    GenPept Link UniProtKB Link
    P33240.1 GenPept UniProtKB/Swiss-Prot:P33240
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    Additional Links

    Additional Links

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

    Chemical Information
    Molecular Biology Databases
    Research Materials
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      Supplemental Content

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