Gls glutaminase [Mus musculus (house mouse)] - Gene

Summary

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Official Symbol: Glsprovided by MGI
Official Full Name: glutaminaseprovided by MGI
Primary source: MGI:95752
See related: Ensembl:ENSMUSG00000026103; Vega:OTTMUSG00000021410
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: AI314027; 6330442B14; B230365M23Rik

Genomic context

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Location :: 1 C1.1; 1 26.86 cM

Sequence :: Chromosome: 1; NC_000067.6 (52163449..52233232, complement)

See Gls in Epigenomics, MapViewer

Chromosome 1 - NC_000067.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

Bibliography

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GeneRIFs: Gene References Into Functions What's a GeneRIF?

identified a novel distant regulatory element located approximately 120kb downstream of the gls promoter, and examined its regulatory relevance to gls gene expression in C2C12 cells
Title: A conserved regulatory element located far downstream of the gls locus modulates gls expression through chromatin loop formation during myogenesis.
In wild-type (WT) mice, GLS1 gene expression was highest in the hippocampus and cortex, where it was reduced by about 50% in Glutaminase-deficient mice.
Title: Synaptic underpinnings of altered hippocampal function in glutaminase-deficient mice during maturation.
GLS1 heterozygous (GLS1 het) mice showed about a 50% global reduction in glutaminase activity and showed hippocampal hypometabolism mainly in the CA1 subregion and subiculum.
Title: Glutaminase-deficient mice display hippocampal hypoactivity, insensitivity to pro-psychotic drugs and potentiated latent inhibition: relevance to schizophrenia.
These results show that necrotic neurons activate in microglia a MyD88-dependent pathway responsible for a pro-inflammatory response that also leads to increased neurotoxic activity through induction of glutaminase.
Title: Necrotic neurons enhance microglial neurotoxicity through induction of glutaminase by a MyD88-dependent pathway.

Submit: New GeneRIF Correction

Alleles

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Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (Floxed/Frt) (2) 1 citation

Pathways from BioSystems

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Alanine, aspartate and glutamate metabolism, organism-specific biosystem (from KEGG)
Alanine, aspartate and glutamate metabolism, organism-specific biosystem
Alanine, aspartate and glutamate metabolism
Alanine, aspartate and glutamate metabolism, conserved biosystem (from KEGG)
Alanine, aspartate and glutamate metabolism, conserved biosystem
Alanine, aspartate and glutamate metabolism
Amino Acid metabolism, organism-specific biosystem (from WikiPathways)
Amino Acid metabolism, organism-specific biosystemA complete overview of the metabolism of all 20 amino acids
Amino acid synthesis and interconversion (transamination), organism-specific biosystem (from REACTOME)
Amino acid synthesis and interconversion (transamination), organism-specific biosystemcomputationally inferred pathway (not manually curated)
Arginine and proline metabolism, organism-specific biosystem (from KEGG)
Arginine and proline metabolism, organism-specific biosystem
Arginine and proline metabolism
Arginine and proline metabolism, conserved biosystem (from KEGG)
Arginine and proline metabolism, conserved biosystem
Arginine and proline metabolism
D-Glutamine and D-glutamate metabolism, organism-specific biosystem (from KEGG)
D-Glutamine and D-glutamate metabolism, organism-specific biosystem
D-Glutamine and D-glutamate metabolism
D-Glutamine and D-glutamate metabolism, conserved biosystem (from KEGG)
D-Glutamine and D-glutamate metabolism, conserved biosystem
D-Glutamine and D-glutamate metabolism
GABAergic synapse, organism-specific biosystem (from KEGG)
GABAergic synapse, organism-specific biosystemGamma aminobutyric acid (GABA) is the most abundant inhibitory neurotransmitter in the mammalian central nervous system (CNS). When released in the synaptic cleft, GABA binds to three major classes o...
GABAergic synapse, conserved biosystem (from KEGG)
GABAergic synapse, conserved biosystemGamma aminobutyric acid (GABA) is the most abundant inhibitory neurotransmitter in the mammalian central nervous system (CNS). When released in the synaptic cleft, GABA binds to three major classes o...
Glutamate Neurotransmitter Release Cycle, organism-specific biosystem (from REACTOME)
Glutamate Neurotransmitter Release Cycle, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Glutamatergic synapse, organism-specific biosystem (from KEGG)
Glutamatergic synapse, organism-specific biosystemGlutamate is the major excitatory neurotransmitter in the mammalian central nervous system(CNS). Glutamate is packaged into synaptic vesicles in the presynaptic terminal. Once released into the synap...
Glutamatergic synapse, conserved biosystem (from KEGG)
Glutamatergic synapse, conserved biosystemGlutamate is the major excitatory neurotransmitter in the mammalian central nervous system(CNS). Glutamate is packaged into synaptic vesicles in the presynaptic terminal. Once released into the synap...
Metabolism, organism-specific biosystem (from REACTOME)
Metabolism, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Metabolism of amino acids and derivatives, organism-specific biosystem (from REACTOME)
Metabolism of amino acids and derivatives, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Neuronal System, organism-specific biosystem (from REACTOME)
Neuronal System, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Neurotransmitter Release Cycle, organism-specific biosystem (from REACTOME)
Neurotransmitter Release Cycle, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Proximal tubule bicarbonate reclamation, organism-specific biosystem (from KEGG)
Proximal tubule bicarbonate reclamation, organism-specific biosystemOne of the major tasks of the renal proximal tubule (PT) is to secrete acid into the tubule lumen, thereby reabsorbing approximately 80% of the filtered bicarbonate (HCO3(-)), as well as generating "...
Proximal tubule bicarbonate reclamation, conserved biosystem (from KEGG)
Proximal tubule bicarbonate reclamation, conserved biosystemOne of the major tasks of the renal proximal tubule (PT) is to secrete acid into the tubule lumen, thereby reabsorbing approximately 80% of the filtered bicarbonate (HCO3(-)), as well as generating "...
Transmission across Chemical Synapses, organism-specific biosystem (from REACTOME)
Transmission across Chemical Synapses, organism-specific biosystemcomputationally inferred pathway (not manually curated)

General gene information

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Markers

Gls (e-PCR), detects polymorphism
- UniSTS:506938

AI314027 (e-PCR)
- UniSTS:158792

Gls (e-PCR), detects polymorphism
- UniSTS:256613

Gls (e-PCR), detects polymorphism
- UniSTS:143224

D1Ncl2 (e-PCR), detects polymorphism
- UniSTS:142261

RH137240 (e-PCR)
- UniSTS:219328

Homology

Homologs of the Gls gene: The Gls gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, rat, chicken, zebrafish, and fruit fly.
Map Viewer (Human, Rat)
OrthoDB: The Hierarchical Catalog of Eukaryotic Orthologs

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
glutaminase activity	IDA Inferred from Direct Assay more info
glutaminase activity	IMP Inferred from Mutant Phenotype more info	PubMed
glutaminase activity	ISO Inferred from Sequence Orthology more info
hydrolase activity	IEA Inferred from Electronic Annotation more info
protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
glutamate biosynthetic process	IDA Inferred from Direct Assay more info
glutamate biosynthetic process	ISO Inferred from Sequence Orthology more info
glutamine catabolic process	IDA Inferred from Direct Assay more info
glutamine catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
glutamine catabolic process	ISO Inferred from Sequence Orthology more info
glutamine metabolic process	IEA Inferred from Electronic Annotation more info
protein homotetramerization	IDA Inferred from Direct Assay more info
protein homotetramerization	ISO Inferred from Sequence Orthology more info
regulation of respiratory gaseous exchange by neurological system process	IMP Inferred from Mutant Phenotype more info	PubMed
suckling behavior	IMP Inferred from Mutant Phenotype more info	PubMed
synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
cytoplasm	IEA Inferred from Electronic Annotation more info
mitochondrion	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: glutaminase kidney isoform, mitochondrial

Names: glutaminase kidney isoform, mitochondrial

NP_001074550.1

EC 3.5.1.2

NP_001106854.1

EC 3.5.1.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001081081.2 → NP_001074550.1 glutaminase kidney isoform, mitochondrial isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AC123752

Consensus CDS

CCDS35561.1

UniProtKB/Swiss-Prot

D3Z7P3

Related

ENSMUSP00000110158, OTTMUSP00000023770, ENSMUST00000114513, OTTMUST00000050744

Conserved Domains (3) summary

cd00204
Location:562 – 647
Blast Score: 95

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

pfam04960
Location:249 – 535
Blast Score: 1289

Glutaminase; Glutaminase

pfam12796
Location:562 – 644
Blast Score: 85

Ank_2; Ankyrin repeats (3 copies)
NM_001113383.1 → NP_001106854.1 glutaminase kidney isoform, mitochondrial isoform 2

Status: VALIDATED

Description

Transcript Variant: This variant (2) includes an alternate exon, compared to variant 1, resulting in a shorter protein (isoform 2) that has a distinct C-terminus, compared to isoform 1.

Source sequence(s)

AK173039, BE849049

Consensus CDS

CCDS48256.1

UniProtKB/Swiss-Prot

D3Z7P3

Conserved Domains (1) summary

pfam04960
Location:249 – 535
Blast Score: 1289

Glutaminase; Glutaminase

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 103

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm38.p1 C57BL/6J

Genomic

NC_000067.6 Reference GRCm38.p1 C57BL/6J

Range

52163449..52233232, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate Mm_Celera

Genomic

AC_000023.1 Alternate Mm_Celera

Range

52701920..52771737, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Nucleotide		Protein	Strain
Heading	Accession and Version	Protein	Strain
genomic	AAHY01006307.1	None
genomic	AAHY01006308.1	None
genomic	AC123752.8 (32862..102645)	None
genomic	AF038395.1	None	129
genomic	AF038396.1	None	129
genomic	AF038397.1	None	129
mRNA	AK031899.1	None	C57BL/6J
mRNA	AK032267.1	None	C57BL/6J
mRNA	AK046297.1	None	C57BL/6J
mRNA	AK048072.1	None	C57BL/6J
mRNA	AK049883.1	None	C57BL/6J
mRNA	AK079838.1	None	C57BL/6J
mRNA	AK088231.1	None	NOD
mRNA	AK141044.1	None	C57BL/6J
mRNA	AK173039.1	BAD32317.1
mRNA	BC025534.1	None
mRNA	BC050208.1	None
mRNA	BE849049.1	None	C57BL/6J
other-genetic	BC169203.1	None