CPB2 carboxypeptidase B2 (plasma) [Homo sapiens] - Gene

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Summary

Official Symbol: CPB2provided by HGNC
Official Full Name: carboxypeptidase B2 (plasma)provided by HGNC
Primary source: HGNC:2300
See related: Ensembl:ENSG00000080618; HPRD:04374; MIM:603101; Vega:OTTHUMG00000016867
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: CPU; PCPB; TAFI
Summary: Carboxypeptidases are enzymes that hydrolyze C-terminal peptide bonds. The carboxypeptidase family includes metallo-, serine, and cysteine carboxypeptidases. According to their substrate specificity, these enzymes are referred to as carboxypeptidase A (cleaving aliphatic residues) or carboxypeptidase B (cleaving basic amino residues). The protein encoded by this gene is activated by trypsin and acts on carboxypeptidase B substrates. After thrombin activation, the mature protein downregulates fibrinolysis. Polymorphisms have been described for this gene and its promoter region. Available sequence data analyses indicate splice variants that encode different isoforms. [provided by RefSeq, Jul 2008]

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Genomic context

Location :: 13q14.11

Sequence :: Chromosome: 13; NC_000013.10 (46627322..46679211, complement)

See CPB2 in Epigenomics, MapViewer

Chromosome 13 - NC_000013.10 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Preeclampsia by itself may be responsible for the increase in TAFIa values rather than the presence of polymorphisms.
Title: Association between genotype and plasma levels of thrombin-activated fibrinolysis inhibitor (TAFI) in the development of preeclampsia.
These findings suggest that plasma carboxypeptidase B plays a critical role in dampening local, C5a-mediated inflammation and represents a molecular link between inflammation and coagulation in autoimmune arthritis.
Title: Plasma carboxypeptidase B downregulates inflammatory responses in autoimmune arthritis.
Recent advances in CPU research include the unravelling of the crystal structure of proCPU and revealing the molecular mechanisms for the marked instability of the active enzyme, CPU. Review.
Title: An update on the role of carboxypeptidase U (TAFIa) in fibrinolysis.
Data provide evidence that TAFI Thr325IIe SNP (rs1926447) could be one of the genetic factors that increas the risk for myocardial infarct in the Egyptian population.
Title: Plasma thrombin-activatable fibrinolysis inhibitor levels and Thr325Ile polymorphism as a risk marker of myocardial infarction in Egyptian patients.
Significantly elevated TAFI levels in patients with multiple myeloma and higher serum TAFI levels were suggested to be associated with higher disease stage.
Title: Serum thrombin activatable fibrinolysis inhibitor levels in patients with newly diagnosed multiple myeloma.
an interaction between TAFIa and plasminogen comprises a component of the reaction mechanism, the plausibility of which was established by showing that TAFIa binds both Glu-Pg and Lys-Pg.
Title: Kinetics of activated thrombin-activatable fibrinolysis inhibitor (TAFIa)-catalyzed cleavage of C-terminal lysine residues of fibrin degradation products and removal of plasminogen-binding sites.
High TAFI levels are associated with increased risk of premature peripheral arterial disease.
Title: High thrombin activatable fibrinolysis inhibitor levels are associated with an increased risk of premature peripheral arterial disease.
the secretion of platelet-derived TAFI can augment the concentrations of TAFI already present in plasma to enhance attenuation of the fibrinolytic cascade
Title: Secretion and antifibrinolytic function of thrombin-activatable fibrinolysis inhibitor from human platelets.
TAFI activation is one major mechanism whereby platelets make clots resistant to fibrinolysis.
Title: The role of thrombin activatable fibrinolysis inhibitor and factor XI in platelet-mediated fibrinolysis resistance: a thromboelastographic study in whole blood.
A higher concentration of TAFI is associated with a worse prognosis in reperfusion & recurrence in acute myocardial infarction treated with fibrinolysis. The C1040T SNP increases TAFI levels. This may encourage clot resistance to thrombolytic agents.
Title: [Prognosis value of thrombin activatable fibrinolysis inhibitor concentration and C1040T polymorphism in acute myocardial infarction treated with fibrinolysis].

Submit: New GeneRIF Correction See all (116)

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Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

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Products	Interactant	Other Gene	Source	Pubs	Description
Q96IY4	P00734	F2	HPRD	PubMed
Q96IY4	P00747	PLG	HPRD	PubMed
Q96IY4	P20742	PZP	HPRD	PubMed
BioGRID:107753	BioGRID:106524	A2M	BioGRID	PubMed	Reconstituted Complex
BioGRID:107753	BioGRID:107188	C5	BioGRID	PubMed	Biochemical Activity
BioGRID:107753	BioGRID:108447	F2	BioGRID	PubMed	Reconstituted Complex
BioGRID:107753	BioGRID:111356	PLG	BioGRID	PubMed	Co-purification
BioGRID:107753	BioGRID:113164	UBC	BioGRID	PubMed	Reconstituted Complex

Function	Evidence Code	Pubs
metal ion binding	IEA Inferred from Electronic Annotation more info
metallocarboxypeptidase activity	IEA Inferred from Electronic Annotation more info
metallopeptidase activity	IEA Inferred from Electronic Annotation more info
peptidase activity	IEA Inferred from Electronic Annotation more info
zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
blood coagulation	IEA Inferred from Electronic Annotation more info
metabolic process	IEA Inferred from Electronic Annotation more info
negative regulation of fibrinolysis	IEA Inferred from Electronic Annotation more info
proteolysis	IEA Inferred from Electronic Annotation more info
response to drug	IEA Inferred from Electronic Annotation more info
response to heat	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
extracellular region	IEA Inferred from Electronic Annotation more info
extracellular space	IEA Inferred from Electronic Annotation more info

General protein information

Preferred Names: carboxypeptidase B2

Names: carboxypeptidase B2; carboxypeptidase U; plasma carboxypeptidase B; carboxypeptidase B-like protein; thrombin-activable fibrinolysis inhibitor; thrombin-activatable fibrinolysis inhibitor; carboxypeptidase B2 (plasma, carboxypeptidase U)

NP_001863.2

EC 3.4.17.20

NP_057497.3

EC 3.4.17.20

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NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001872.3 → NP_001863.2 carboxypeptidase B2 isoform a preproprotein

Status: REVIEWED

Description

Transcript Variant: This variant (1) encodes the full-length protein.

Source sequence(s)

AL137141, AL157758, M75106

Consensus CDS

CCDS9401.1

UniProtKB/Swiss-Prot

Q96IY4

Related

ENSP00000181383, OTTHUMP00000018364, ENST00000181383, OTTHUMT00000044803

Conserved Domains (2) summary

pfam02244
Location:33 – 105
Blast Score: 154

Propep_M14; Carboxypeptidase activation peptide

cd06246
Location:119 – 420
Blast Score: 1560

M14_CPB2; Peptidase M14 carboxypeptidase subfamily A/B-like; Carboxypeptidase B2 subgroup
NM_016413.3 → NP_057497.3 carboxypeptidase B2 isoform b precursor

Status: REVIEWED

Description

Transcript Variant: This variant (2) lacks two segments in the coding region when compared to variant 1. The absence of the second segment causes a frameshift; therefore, isoform b lacks an internal aa fragment and has a distinct C-terminus when compared to isoform a.

Source sequence(s)

AL137141, AL157758, M75106

Consensus CDS

CCDS9402.1

UniProtKB/Swiss-Prot

Q96IY4

Related

ENSP00000400714, OTTHUMP00000018365, ENST00000439329, OTTHUMT00000044804

Conserved Domains (2) summary

pfam02244
Location:33 – 105
Blast Score: 153

Propep_M14; Carboxypeptidase activation peptide

cl11393
Location:119 – 346
Blast Score: 1150

Peptidase_M14_like; M14 family of metallocarboxypeptidases and related proteins

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000013.10 Reference GRCh37.p5 Primary Assembly

Range

46627322..46679211, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000145.1 Alternate HuRef

Range

27424287..27477016, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

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Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AF080222.1	None
genomic	AF080223.1	None
genomic	AL137141.10	CAI12170.1
		CAI12171.1
genomic	AL157758.10	CAI10904.1
		CAI10905.1
genomic	AY714780.1	AAT97987.1
genomic	CH471075.1	EAX08754.1
		EAX08755.1
mRNA	AA700377.1	None
mRNA	AB011969.1	BAA90475.1
mRNA	AK290829.1	BAF83518.1
mRNA	AV719308.1	None
mRNA	BC007057.1	AAH07057.1
mRNA	BT006936.1	AAP35582.1
mRNA	M75106.1	AAA60042.1
other-genetic	DQ890567.2	ABM92184.1
other-genetic	EU176478.1	ABW03279.1