Cd247 CD247 antigen [Mus musculus (house mouse)] - Gene

Summary

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Official Symbol: Cd247provided by MGI
Official Full Name: CD247 antigenprovided by MGI
Primary source: MGI:88334
See related: Ensembl:ENSMUSG00000005763; Vega:OTTMUSG00000034863
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Cd3; T3z; Cd3h; Cd3z; Tcrk; Tcrz; Cd3-eta; Cd3zeta; AW552088; Cd3-zeta; 4930549J05Rik; A430104F18Rik

Genomic context

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Location :: 1 G-H; 1 73.14 cM

Sequence :: Chromosome: 1; NC_000067.6 (165788681..165871153)

See Cd247 in Epigenomics, MapViewer

Chromosome 1 - NC_000067.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

Bibliography

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GeneRIFs: Gene References Into Functions What's a GeneRIF?

Tyrosine phosphorylation of the TCR-zeta cytoplasmic domain regulates its association with the plasma membrane
Title: Basic residues in the T-cell receptor ζ cytoplasmic domain mediate membrane association and modulate signaling.
The data of this study suggested that CD3zeta mediates the ZAP-70/Syk kinase activation triggered by ephrinA-activated pathway to regulate early neuronal morphogenesis.
Title: The immune molecule CD3zeta and its downstream effectors ZAP-70/Syk mediate ephrin signaling in neurons to regulate early neuritogenesis.
Data show that TCRzeta phosphorylation signal pathways were affected in CD3gamma(-/-) primary and HVS-transformed T cells.
Title: CD3γ-independent pathways in TCR-mediated signaling in mature T and iNKT lymphocytes.
functional role for CD3 zeta basic-rich stretch -phosphoinositide interactions in supporting T cell activation
Title: The CD3 zeta subunit contains a phosphoinositide-binding motif that is required for the stable accumulation of TCR-CD3 complex at the immunological synapse.
Results suggest that RhoH regulates TCR signaling via recruitment of ZAP-70 and Lck to CD3zeta in the immunological synapse.
Title: RhoH regulates subcellular localization of ZAP-70 and Lck in T cell receptor signaling.
an intracellular pool of phosphorylated CD3zeta may help to sustain TcR/CD3 signaling after the receptor internalization.
Title: Imaging T-cell receptor activation reveals accumulation of tyrosine-phosphorylated CD3ζ in the endosomal compartment.
The allele of the Cd3zeta gene expressed in NOD and DBA/2 mouse strains confers lower levels of T cell activation compared with the allele expressed by C57BL/6, BALB/c, and C3H/HeJ mice.
Title: Variation in the Cd3 zeta (Cd247) gene correlates with altered T cell activation and is associated with autoimmune diabetes.
GRAIL, by mediating TCR-CD3 degradation, regulates naive T cell tolerance induction and Treg cell function
Title: The E3 ubiquitin ligase GRAIL regulates T cell tolerance and regulatory T cell function by mediating T cell receptor-CD3 degradation.
a full complement of functional CD3 zeta immunoreceptor tyrosine-based activation motifs is required for effective iNKT cell development.
Title: Invariant NKT cell development requires a full complement of functional CD3 zeta immunoreceptor tyrosine-based activation motifs.
We show that CD3zeta is expressed in retinal ganglion cells (RGCs). CD3zeta-deficient mice have reduced RGC dendritic motility, an increase in RGC dendritic density, and a selective defect of glutamate-receptor-mediated synaptic activity in the retina.
Title: The immune protein CD3zeta is required for normal development of neural circuits in the retina.

Submit: New GeneRIF Correction See all (26)

Alleles

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Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (knock-out) (5) 1 citation
Chemically induced (ENU) (1)
Targeted (knock-in) (2) 1 citation

Interactions

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Products	Interactant	Other Gene	Source	Pubs	Description
NP_112439.1	NP_112439.1	Cd247	BIND	PubMed	CD3-zeta interacts with CD3-zeta to form a dimer.
NP_112439.1	NP_034823.1	Lck	BIND	PubMed	p56Lck interacts with, and phosphorylates, TCR-zeta.
BioGRID:198598	BioGRID:204063	Tcrb	BioGRID	PubMed	Affinity Capture-Western
BioGRID:198598	BioGRID:204500	Vav1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:198598	BioGRID:204628	Zap70	BioGRID	PubMed	Affinity Capture-Western

Pathways from BioSystems

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Adaptive Immune System, organism-specific biosystem (from REACTOME)
Adaptive Immune System, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Chagas disease (American trypanosomiasis), organism-specific biosystem (from KEGG)
Chagas disease (American trypanosomiasis), organism-specific biosystemTrypanosoma cruzi is an intracellular protozoan parasite that causes Chagas disease. The parasite life cycle involves hematophagous reduviid bugs as vectors. Once parasites enter the host body, they ...
Chagas disease (American trypanosomiasis), conserved biosystem (from KEGG)
Chagas disease (American trypanosomiasis), conserved biosystemTrypanosoma cruzi is an intracellular protozoan parasite that causes Chagas disease. The parasite life cycle involves hematophagous reduviid bugs as vectors. Once parasites enter the host body, they ...
Costimulation by the CD28 family, organism-specific biosystem (from REACTOME)
Costimulation by the CD28 family, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Downstream TCR signaling, organism-specific biosystem (from REACTOME)
Downstream TCR signaling, organism-specific biosystemcomputationally inferred pathway (not manually curated)
FCGR activation, organism-specific biosystem (from REACTOME)
FCGR activation, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Fcgamma receptor (FCGR) dependent phagocytosis, organism-specific biosystem (from REACTOME)
Fcgamma receptor (FCGR) dependent phagocytosis, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Generation of second messenger molecules, organism-specific biosystem (from REACTOME)
Generation of second messenger molecules, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Immune System, organism-specific biosystem (from REACTOME)
Immune System, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell, organism-specific biosystem (from REACTOME)
Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Innate Immune System, organism-specific biosystem (from REACTOME)
Innate Immune System, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Natural killer cell mediated cytotoxicity, organism-specific biosystem (from KEGG)
Natural killer cell mediated cytotoxicity, organism-specific biosystemNatural killer (NK) cells are lymphocytes of the innate immune system that are involved in early defenses against both allogeneic (nonself) cells and autologous cells undergoing various forms of stre...
Natural killer cell mediated cytotoxicity, conserved biosystem (from KEGG)
Natural killer cell mediated cytotoxicity, conserved biosystemNatural killer (NK) cells are lymphocytes of the innate immune system that are involved in early defenses against both allogeneic (nonself) cells and autologous cells undergoing various forms of stre...
PD-1 signaling, organism-specific biosystem (from REACTOME)
PD-1 signaling, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Phosphorylation of CD3 and TCR zeta chains, organism-specific biosystem (from REACTOME)
Phosphorylation of CD3 and TCR zeta chains, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Regulation of actin dynamics for phagocytic cup formation, organism-specific biosystem (from REACTOME)
Regulation of actin dynamics for phagocytic cup formation, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Role of phospholipids in phagocytosis, organism-specific biosystem (from REACTOME)
Role of phospholipids in phagocytosis, organism-specific biosystemcomputationally inferred pathway (not manually curated)
T Cell Receptor Signaling Pathway, organism-specific biosystem (from WikiPathways)
T Cell Receptor Signaling Pathway, organism-specific biosystem
T Cell Receptor Signaling Pathway
T cell receptor signaling pathway, organism-specific biosystem (from KEGG)
T cell receptor signaling pathway, organism-specific biosystemActivation of T lymphocytes is a key event for an efficient response of the immune system. It requires the involvement of the T-cell receptor (TCR) as well as costimulatory molecules such as CD28. En...
T cell receptor signaling pathway, conserved biosystem (from KEGG)
T cell receptor signaling pathway, conserved biosystemActivation of T lymphocytes is a key event for an efficient response of the immune system. It requires the involvement of the T-cell receptor (TCR) as well as costimulatory molecules such as CD28. En...
TCR signaling, organism-specific biosystem (from REACTOME)
TCR signaling, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Translocation of ZAP-70 to Immunological synapse, organism-specific biosystem (from REACTOME)
Translocation of ZAP-70 to Immunological synapse, organism-specific biosystemcomputationally inferred pathway (not manually curated)
XPodNet - protein-protein interactions in the podocyte expanded by STRING, organism-specific biosystem (from WikiPathways)
XPodNet - protein-protein interactions in the podocyte expanded by STRING, organism-specific biosystemXPodNet is a larger version of PodNet. Interactions from STRING database have been added to PodNet to build XPodNet. Hence, not all interactions found in XPodNet do necessarily have to be curated.

General gene information

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Markers

AW552088 (e-PCR)
- UniSTS:178499

J04967 (e-PCR)
- UniSTS:178503

RH94658 (e-PCR)
- UniSTS:119905

J03297 (e-PCR)
- UniSTS:124852

L03353 (e-PCR)
- UniSTS:163269

Cd3z (e-PCR), detects polymorphism
- UniSTS:141281

D1Mit144 (e-PCR), detects polymorphism
- UniSTS:128481

PMC197251P5 (e-PCR)
- UniSTS:271854

Homology

Homologs of the Cd247 gene: The Cd247 gene is conserved in human, chimpanzee, Rhesus monkey, cow, rat, and chicken.
Map Viewer (Human, Rat)
OrthoDB: The Hierarchical Catalog of Eukaryotic Orthologs

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
identical protein binding	ISO Inferred from Sequence Orthology more info
protein binding	IPI Inferred from Physical Interaction more info	PubMed
transmembrane signaling receptor activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
T cell receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
cell surface receptor signaling pathway	ISO Inferred from Sequence Orthology more info
signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
T cell receptor complex	ISO Inferred from Sequence Orthology more info
alpha-beta T cell receptor complex	IDA Inferred from Direct Assay more info	PubMed
cytoplasm	ISO Inferred from Sequence Orthology more info
integral to membrane	IEA Inferred from Electronic Annotation more info
membrane	IEA Inferred from Electronic Annotation more info
plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: CD247 antigen

Names: CD247 antigen; CD3-zeta/eta; T-cell receptor T3 eta chain; CD3 antigen, zeta polypeptide; T-cell surface glycoprotein CD3 zeta chain

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001113391.2 → NP_001106862.1 CD247 antigen isoform zeta precursor

Status: VALIDATED

Description

Transcript Variant: This variant (zeta) encodes the shortest isoform (zeta).

Source sequence(s)

AC093371, AK138774, BC052824, BM240376

Consensus CDS

CCDS48427.1

UniProtKB/Swiss-Prot

P24161

UniProtKB/TrEMBL

Q3UU54

Related

ENSMUSP00000005907, OTTMUSP00000048312, ENSMUST00000005907, OTTMUST00000088615

Conserved Domains (2) summary

pfam02189
Location:69 – 89
Blast Score: 80

ITAM; Immunoreceptor tyrosine-based activation motif

pfam11628
Location:28 – 59
Blast Score: 165

TCR_zetazeta; T-cell surface glycoprotein CD3 zeta chain
NM_001113392.2 → NP_001106863.1 CD247 antigen isoform iota precursor

Status: VALIDATED

Description

Transcript Variant: This variant (iota) has alternate exon structure in the 3' coding region and 3' UTR, compared to variant zeta. The resulting isoform (iota) has a distinct C-terminus and is longer than isoform zeta.

Source sequence(s)

AC093371, AK138774

Consensus CDS

CCDS48429.1

UniProtKB/TrEMBL

Q3UU54

Related

ENSMUSP00000124297, OTTMUSP00000048309, ENSMUST00000161971, OTTMUST00000088612

Conserved Domains (2) summary

pfam02189
Location:69 – 89
Blast Score: 81

ITAM; Immunoreceptor tyrosine-based activation motif

pfam11628
Location:28 – 59
Blast Score: 166

TCR_zetazeta; T-cell surface glycoprotein CD3 zeta chain
NM_001113393.2 → NP_001106864.2 CD247 antigen isoform theta precursor

Status: VALIDATED

Description

Transcript Variant: This variant (theta) uses an alternate in-frame splice site in the central coding region, and has alternate exon structure in the 3' coding region and 3' UTR, compared to variant zeta. The resulting isoform (theta) has a distinct C-terminus and is longer than isoform zeta.

Source sequence(s)

AC093371, AC124587

Consensus CDS

CCDS48428.2

UniProtKB/TrEMBL

A2MZG6

Related

ENSMUSP00000136456, ENSMUST00000178336

Conserved Domains (2) summary

pfam02189
Location:69 – 89
Blast Score: 83

ITAM; Immunoreceptor tyrosine-based activation motif

pfam11628
Location:28 – 59
Blast Score: 168

TCR_zetazeta; T-cell surface glycoprotein CD3 zeta chain
NM_001113394.2 → NP_001106865.1 CD247 antigen isoform eta precursor

Status: VALIDATED

Description

Transcript Variant: This variant (kappa) has alternate exon structure in the 3' coding region and 3' UTR, compared to variant zeta. The resulting isoform (eta) has a distinct C-terminus and is longer than isoform zeta. Variants eta and kappa encode the same isoform.

Source sequence(s)

AC093371, AC124587

Consensus CDS

CCDS15443.1

UniProtKB/Swiss-Prot

P29020

Conserved Domains (2) summary

pfam02189
Location:69 – 89
Blast Score: 82

ITAM; Immunoreceptor tyrosine-based activation motif

pfam11628
Location:28 – 59
Blast Score: 170

TCR_zetazeta; T-cell surface glycoprotein CD3 zeta chain
NM_031162.4 → NP_112439.1 CD247 antigen isoform eta precursor

Status: VALIDATED

Description

Transcript Variant: This variant (eta) has alternate exon structure in the 3' coding region and 3' UTR, compared to variant zeta. The resulting isoform (eta) has a distinct C-terminus and is longer than isoform zeta. Variants eta and kappa encode the same isoform.

Source sequence(s)

AC093371, AC124587, BF020526

Consensus CDS

CCDS15443.1

UniProtKB/Swiss-Prot

P29020

Related

ENSMUSP00000027849, OTTMUSP00000048311, ENSMUST00000027849, OTTMUST00000088614

Conserved Domains (2) summary

pfam02189
Location:69 – 89
Blast Score: 82

ITAM; Immunoreceptor tyrosine-based activation motif

pfam11628
Location:28 – 59
Blast Score: 170

TCR_zetazeta; T-cell surface glycoprotein CD3 zeta chain

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 103

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm38.p1 C57BL/6J

Genomic

NC_000067.6 Reference GRCm38.p1 C57BL/6J

Range

165788681..165871153

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate Mm_Celera

Genomic

AC_000023.1 Alternate Mm_Celera

Range

168233341..168317001

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Nucleotide		Protein	Strain
Heading	Accession and Version	Protein	Strain
genomic	AAHY01000993.1	None
genomic	AAHY01000994.1	None
genomic	AAHY01000995.1	None
genomic	AAHY01000996.1	None
genomic	AAHY01000997.1	None
genomic	AAHY01000998.1	None
genomic	AAHY01000999.1 (22264..23815)	None
genomic	AC090495.5 (49800..82400)	None
genomic	AC093371.6 (132572..175869)	None
genomic	AC124587.5	None
genomic	CH466520.2	EDL39214.1	mixed
		EDL39215.1	mixed
genomic	M19729.1	AAA40171.1
genomic	M76711.1	AAA40403.1
genomic	U17267.1	AAB61085.1
mRNA	AK016078.1	None	C57BL/6J
mRNA	AK017904.1	BAB30997.1	C57BL/6J
mRNA	AK080019.1	None	C57BL/6J
mRNA	AK088087.1	None	NOD
mRNA	AK138774.1	BAE23774.1	C57BL/6J
mRNA	AK139276.1	None	C57BL/6J
mRNA	BC052824.1	AAH52824.1	C57BL/6NCrl
mRNA	BF020526.1	None	C57BL/6J
mRNA	BM240376.1	None	C57BL/6NCrl
mRNA	J04967.1	AAA50301.1
mRNA	L03353.1	AAA37401.1	B6 x CBA/J F1
mRNA	M33158.1	AAA37398.1
mRNA	S51932.1	AAB24559.1
mRNA	U56258.1	AAD11545.1	C3H/HeN
mRNA	X84237.1	CAA59015.1	C3H/HeN