CHEK2 checkpoint kinase 2 [Homo sapiens (human)] - Gene

Summary

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Official Symbol: CHEK2provided by HGNC
Official Full Name: checkpoint kinase 2provided by HGNC
Primary source: HGNC:16627
Locus tag: RP11-436C9.1
See related: Ensembl:ENSG00000183765; HPRD:05084; MIM:604373; Vega:OTTHUMG00000151023
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425
Summary: In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Genomic context

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Location :: 22q12.1

Sequence :: Chromosome: 22; NC_000022.10 (29083731..29137822, complement)

See CHEK2 in Epigenomics, MapViewer

Chromosome 22 - NC_000022.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

Bibliography

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GeneRIFs: Gene References Into Functions What's a GeneRIF?

Among women with estrogen receptor-positive breast cancer, CHEK2*1100delC heterozygosity was associated with a 1.4-fold risk of early death, a 1.6-fold risk of breast cancer-specific death, and a 3.5-fold risk of a second breast cancer.
Title: CHEK2*1100delC heterozygosity in women with breast cancer associated with early death, breast cancer-specific death, and increased risk of a second breast cancer.
Higher phosphorylation levels of CHK2 is associated with poor treatment response in rectal cancer.
Title: Multiplexed protein signal pathway mapping identifies patients with rectal cancer that responds to neoadjuvant treatment.
HIF-2alpha is involved in the blocking effects of arsenite on activation of the ATM/Chk-2 pathway and in repair of DNA damage induced by BaP in HBE cells.
Title: The inhibition of HIF-2α on the ATM/Chk-2 pathway is involved in the promotion effect of arsenite on benzo(a)pyrene-induced cell transformation.
loss of function of the ATM-Chek2-p53 cascade is strongly associated with resistance to anthracycline/mitomycin-containing chemotherapy in breast cancer
Title: Low expression levels of ATM may substitute for CHEK2 /TP53 mutations predicting resistance towards anthracycline and mitomycin chemotherapy in breast cancer.
MYC regulation of CHK1 and CHK2 promotes radioresistance in a stem cell-like population of nasopharyngeal carcinoma cells.
Title: MYC regulation of CHK1 and CHK2 promotes radioresistance in a stem cell-like population of nasopharyngeal carcinoma cells.
Five percent of women with uterine serous carcinoma have germline mutations the tumor suppressor genes BRCA1, CHEK2, and TP53.
Title: BRCA1, TP53, and CHEK2 germline mutations in uterine serous carcinoma.
Our research indicates that the CHEK2 I157T variant may be another important genetic mutation which increases risk of breast cancer, especially the lobular type.
Title: The CHEK2 I157T variant and breast cancer susceptibility: a systematic review and meta-analysis.
CHK2 1100delC, IVS2+1G>A and I157T mutations are not present in hepatocellular cancer cases from a Turkish population.
Title: CHK2 1100delC, IVS2+1G>A and I157T mutations are not present in hepatocellular cancer cases from a Turkish population.
data indicate the significance of CHEK2 gene alterations in contrast to promoter hypermethylation in breast cancerogenesis
Title: CHEK2 gene alterations independently increase the risk of death from breast cancer in Bulgarian patients.
The CHEK2 I157T variant is associated with colorectal cancer susceptibility.
Title: The CHEK2 I157T variant and colorectal cancer susceptibility: a systematic review and meta-analysis.

Submit: New GeneRIF Correction See all (275)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

A germline mutation in BRCA1 or BRCA2 predisposes to breast and ovarian cancer as well as other cancers. The risk of developing cancer that is associated with a germline BRCA1 or BRCA2 mutation, which has been derived from families with multiple affected individuals, families with few affected individuals, and from population-based studies, appears to be variable within families. Prognosis for breast and ovarian cancer depends on the stage at which the cancer is diagnosed; however, studies on survival have revealed conflicting results for individuals with germline BRCA1 or BRCA2 mutations when compared to controls.

Diagnosis Testing

Molecular genetic testing for germline BRCA1 and BRCA2 mutations is available on a clinical basis for individuals who are identified to be at high risk based on their personal and/or family history and for at-risk relatives of an individual with an identified germline BRCA1 or BRCA2 mutation. No currently available technique can guarantee the identification of all cancer-predisposing mutations in BRCA1 or BRCA2. Furthermore, mutations of uncertain clinical significance may be identified.

Genetic Counseling

Germline mutations in BRCA1 and BRCA2 are inherited in an autosomal dominant manner. Each offspring of an individual with a BRCA1 or BRCA2 germline mutation has a 50% chance of inheriting the mutation. Molecular genetic testing of asymptomatic family members at risk of inheriting either a BRCA1 or BRCA2 germline mutation is feasible once the family-specific mutation has been identified. Prenatal testing is possible for pregnancies at increased risk if the cancer-predisposing mutation in the family is known; however, requests for prenatal diagnosis of adult-onset diseases are uncommon and require careful genetic counseling.

NHGRI GWAS Catalog

A genome-wide association study of optic disc parameters.

A shared susceptibility locus in PLCE1 at 10q23 for gastric adenocarcinoma and esophageal squamous cell carcinoma.

Genetic variants associated with breast size also influence breast cancer risk.

HIV-1 protein interactions

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Protein	Gene	Interaction	Pubs
Env, gp160, envelope glycoprotein	env	PML, TopBP1, NBS1 or ATM-induced activation of phosphorylation of Chk2 participates in the DNA damage-elicited pro-apoptotic cascade that leads to the demise of Env-elicited syncytia	PubMed
Vpr, p15	vpr	Vpr-dependent induction and Vif-mediated attenuation of NKG2D ligands are required for Chk2 phosphorylation in HIV infection	PubMed
	vpr	HIV-1 Vpr induces expression of gamma-H2AX and phosphorylation of Chk2	PubMed

Go to the HIV-1, Human Protein Interaction Database

Interactions

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Products	Interactant	Other Gene	Source	Pubs	Description
NP_009125.1	NP_000042.2	ATM	BIND	PubMed	ATM phosphorylates Chk2 at threonine 68.
NP_009125.1	NP_000042.2	ATM	BIND	PubMed	ATM phosphorylates Chk2 at threonine 68.
O96017	Q9NY61	AATF	HPRD	PubMed
O96017	Anti-silencing function 1A	ASF1A	HPRD	PubMed
O96017	Q13315	ATM	HPRD	PubMed
O96017	Q13315	ATM	HPRD	PubMed
O96017	Q13535	ATR	HPRD	PubMed
O96017	P38398	BRCA1	HPRD	PubMed
O96017	P30304	CDC25A	HPRD	PubMed
O96017	P30307	CDC25C	HPRD	PubMed
O96017	Q9UBU7	DBF4	HPRD	PubMed
O96017	Q01094	E2F1	HPRD	PubMed
O96017	Q03468	ERCC6	HPRD	PubMed
O96017	P52292	KPNA2	HPRD	PubMed
O96017	Q14676	MDC1	HPRD	PubMed
O96017	Q00987	MDM2	HPRD	PubMed
O96017	O15151	MDM4	HPRD	PubMed
O96017	P22897	MRC1	HPRD	PubMed
O96017	P43246	MSH2	HPRD	PubMed
O96017	Q96NY9	MUS81	HPRD	PubMed
O96017	O60934	NBN	HPRD	PubMed
O96017	P53350	PLK1	HPRD	PubMed
O96017	Q9H4B4	PLK3	HPRD	PubMed
O96017	P63151	PPP2R2A	HPRD	PubMed
O96017	Q00005	PPP2R2B	HPRD	PubMed
O96017	Q15172	PPP2R5A	HPRD	PubMed
O96017	Q15173	PPP2R5B	HPRD	PubMed
O96017	Q13362	PPP2R5C	HPRD	PubMed
O96017	Q14738	PPP2R5D	HPRD	PubMed
O96017	Q16537	PPP2R5E	HPRD	PubMed
O96017	Q99638	RAD9A	HPRD	PubMed
O96017	P04637	TP53	HPRD	PubMed
O96017	Q9BXA7	TSSK1B	HPRD	PubMed
BioGRID:116369	BioGRID:117743	AATF	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex
BioGRID:116369	BioGRID:106848	APP	BioGRID	PubMed	Reconstituted Complex
BioGRID:116369	BioGRID:106885	ARHGAP1	BioGRID	PubMed	Two-hybrid
BioGRID:116369	BioGRID:106962	ATM	BioGRID	PubMed	Biochemical Activity; Reconstituted Complex
	AAH02701.1	ATMIN	BIND	PubMed	ASCIZ interacts with an unspecified isoform of CHK2.
BioGRID:116369	BioGRID:107027	ATR	BioGRID	PubMed	Biochemical Activity
BioGRID:116369	BioGRID:107140	BRCA1	BioGRID	PubMed	Affinity Capture-Western; Biochemical Activity; Reconstituted Complex
BioGRID:116369	BioGRID:107142	BRCA2	BioGRID	PubMed	Affinity Capture-Western; Protein-peptide
BioGRID:116369	BioGRID:107428	CDC25A	BioGRID	PubMed	Biochemical Activity; Reconstituted Complex
BioGRID:116369	BioGRID:107430	CDC25C	BioGRID	PubMed	Biochemical Activity
BioGRID:116369	BioGRID:116369	CHEK2	BioGRID	PubMed	Affinity Capture-Western; Biochemical Activity; Co-crystal Structure; Reconstituted Complex
BioGRID:116369	BioGRID:114032	CUL1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:116369	BioGRID:114029	CUL4A	BioGRID	PubMed	Affinity Capture-Western
BioGRID:116369	BioGRID:206143	Chek2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:116369	BioGRID:107983	DAPK3	BioGRID	PubMed	Biochemical Activity
BioGRID:116369	BioGRID:971804	EBNA-3A	BioGRID	PubMed	Affinity Capture-Western
BioGRID:116369	BioGRID:108309	ELAVL1	BioGRID	PubMed	Affinity Capture-RNA; Affinity Capture-Western; Biochemical Activity; Two-hybrid
BioGRID:116369	BioGRID:108594	FOXM1	BioGRID	PubMed	Biochemical Activity
BioGRID:116369	BioGRID:119664	GINS2	BioGRID	PubMed	Affinity Capture-Western
BioGRID:116369	BioGRID:119613	HDAC7	BioGRID	PubMed	Negative Genetic
BioGRID:116369	BioGRID:120968	HDAC8	BioGRID	PubMed	Negative Genetic
BioGRID:116369	BioGRID:114375	KAT2B	BioGRID	PubMed	Affinity Capture-Western
BioGRID:116369	BioGRID:110036	KPNA2	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex; Two-hybrid
BioGRID:116369	BioGRID:117727	LATS2	BioGRID	PubMed	Biochemical Activity
BioGRID:116369	BioGRID:115014	MDC1	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex
BioGRID:116369	BioGRID:110358	MDM2	BioGRID	PubMed	Affinity Capture-Western; Biochemical Activity; Reconstituted Complex
BioGRID:116369	BioGRID:110359	MDM4	BioGRID	PubMed	Biochemical Activity
BioGRID:116369	BioGRID:110573	MSH2	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex
BioGRID:116369	BioGRID:123170	MUS81	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex
BioGRID:116369	BioGRID:109407	NR4A1	BioGRID	PubMed	Two-hybrid
BioGRID:116369	BioGRID:114386	PER3	BioGRID	PubMed	Affinity Capture-Western
BioGRID:116369	BioGRID:111362	PLK1	BioGRID	PubMed	Affinity Capture-Western; Biochemical Activity
BioGRID:116369	BioGRID:107663	PLK3	BioGRID	PubMed	Affinity Capture-Western; Biochemical Activity
BioGRID:116369	BioGRID:111384	PML	BioGRID	PubMed	Affinity Capture-Western; Biochemical Activity
BioGRID:116369	BioGRID:111437	POLR2L	BioGRID	PubMed	Two-hybrid
BioGRID:116369	BioGRID:111507	PPP2CA	BioGRID	PubMed	Affinity Capture-Western
BioGRID:116369	BioGRID:111510	PPP2R1A	BioGRID	PubMed	Affinity Capture-Western
BioGRID:116369	BioGRID:111513	PPP2R2B	BioGRID	PubMed	Two-hybrid
BioGRID:116369	BioGRID:111517	PPP2R5A	BioGRID	PubMed	Reconstituted Complex; Two-hybrid
BioGRID:116369	BioGRID:111518	PPP2R5B	BioGRID	PubMed	Reconstituted Complex
BioGRID:116369	BioGRID:111519	PPP2R5C	BioGRID	PubMed	Affinity Capture-Western; Biochemical Activity; Reconstituted Complex; Two-hybrid
BioGRID:116369	BioGRID:111520	PPP2R5D	BioGRID	PubMed	Reconstituted Complex; Two-hybrid
BioGRID:116369	BioGRID:111521	PPP2R5E	BioGRID	PubMed	Reconstituted Complex; Two-hybrid
BioGRID:116369	BioGRID:111577	PRKDC	BioGRID	PubMed	Biochemical Activity
BioGRID:116369	BioGRID:115492	PSME3	BioGRID	PubMed	Affinity Capture-Western
BioGRID:116369	BioGRID:115417	RAD50	BioGRID	PubMed	Affinity Capture-Western
BioGRID:116369	BioGRID:111860	RB1	BioGRID	PubMed	Affinity Capture-Western; Biochemical Activity; Reconstituted Complex
BioGRID:116369	BioGRID:117406	RCHY1	BioGRID	PubMed	Affinity Capture-Western; Biochemical Activity
BioGRID:116369	BioGRID:111912	REV3L	BioGRID	PubMed	Biochemical Activity
BioGRID:116369	BioGRID:114492	RNF8	BioGRID	PubMed	Affinity Capture-Western; Biochemical Activity
BioGRID:116369	BioGRID:116980	SIRT5	BioGRID	PubMed	Negative Genetic
BioGRID:116369	BioGRID:112649	STAT1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:116369	BioGRID:116342	STRAP	BioGRID	PubMed	Biochemical Activity
BioGRID:116369	BioGRID:113010	TP53	BioGRID	PubMed	Affinity Capture-Western; Biochemical Activity
BioGRID:116369	BioGRID:113011	TP53BP1	BioGRID	PubMed	Affinity Capture-Western
BioGRID:116369	BioGRID:115457	TRIM28	BioGRID	PubMed	Affinity Capture-Western
BioGRID:116369	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-Western
BioGRID:116369	BioGRID:119501	UBR5	BioGRID	PubMed	Affinity Capture-MS; Affinity Capture-Western
BioGRID:116369	BioGRID:113258	VCP	BioGRID	PubMed	Affinity Capture-Western
BioGRID:116369	BioGRID:113269	VHL	BioGRID	PubMed	Affinity Capture-Western; Biochemical Activity

Pathways from BioSystems

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Cell Cycle, organism-specific biosystem (from REACTOME)
Cell Cycle, organism-specific biosystem
Cell Cycle
Cell Cycle Checkpoints, organism-specific biosystem (from REACTOME)
Cell Cycle Checkpoints, organism-specific biosystemA hallmark of the human cell cycle in normal somatic cells is its precision. This remarkable fidelity is achieved by a number of signal transduction pathways, known as checkpoints, which monitor cell...
Cell cycle, organism-specific biosystem (from WikiPathways)
Cell cycle, organism-specific biosystemThe cell cycle is the series of events that takes place in a cell leading to its division and duplication (replication). Regulation of the cell cycle involves processes crucial to the survival of a c...
Cell cycle, organism-specific biosystem (from KEGG)
Cell cycle, organism-specific biosystemMitotic cell cycle progression is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps known as G1 and G2 phases. Cycli...
Cell cycle, conserved biosystem (from KEGG)
Cell cycle, conserved biosystemMitotic cell cycle progression is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps known as G1 and G2 phases. Cycli...
DNA damage response, organism-specific biosystem (from WikiPathways)
DNA damage response, organism-specific biosystemThis is the first pathway out of two pathways which deals with DNA damage response. It has two central gene products (ATM and ATR) which are connected to the sources of DNA damage (in blue). The two ...
FOXM1 transcription factor network, organism-specific biosystem (from Pathway Interaction Database)
FOXM1 transcription factor network, organism-specific biosystem
FOXM1 transcription factor network
G1/S DNA Damage Checkpoints, organism-specific biosystem (from REACTOME)
G1/S DNA Damage Checkpoints, organism-specific biosystemIn the G1 phase there are two types of DNA damage responses, the p53-dependent and the p53-independent pathways. The p53-dependent responses inhibit CDKs through the up-regulation of genes encoding ...
G2/M Checkpoints, organism-specific biosystem (from REACTOME)
G2/M Checkpoints, organism-specific biosystemG2/M checkpoints include the checks for damaged DNA, unreplicated DNA, and checks that ensure that the genome is replicated once and only once per cell cycle. If cells pass these checkpoints, they f...
G2/M DNA damage checkpoint, organism-specific biosystem (from REACTOME)
G2/M DNA damage checkpoint, organism-specific biosystemThroughout the cell cycle, the genome is constantly monitored for damage, resulting either from errors of replication, by-products of metabolism or through extrinsic sources such as ultra-violet or i...
HTLV-I infection, organism-specific biosystem (from KEGG)
HTLV-I infection, organism-specific biosystemHuman T-lymphotropic virus type 1 (HTLV-1) is a pathogenic retrovirus that is associated with adult T-cell leukemia/lymphoma (ATL). It is also strongly implicated in non-neoplastic chronic inflammato...
HTLV-I infection, conserved biosystem (from KEGG)
HTLV-I infection, conserved biosystemHuman T-lymphotropic virus type 1 (HTLV-1) is a pathogenic retrovirus that is associated with adult T-cell leukemia/lymphoma (ATL). It is also strongly implicated in non-neoplastic chronic inflammato...
Integrated Breast Cancer Pathway, organism-specific biosystem (from WikiPathways)
Integrated Breast Cancer Pathway, organism-specific biosystemThis pathway incorporates the most important proteins for Breast Cancer. The Rp score from the Connectivity-Maps (C-Maps) webserver was used to determine the rank of the most important proteins in Br...
Integrated Cancer pathway, organism-specific biosystem (from WikiPathways)
Integrated Cancer pathway, organism-specific biosystem
Integrated Cancer pathway
Integrated Pancreatic Cancer Pathway, organism-specific biosystem (from WikiPathways)
Integrated Pancreatic Cancer Pathway, organism-specific biosystemAn integrated pathway model which displays the protein-protein interactions (PPIs) among the relevant proteins for pancreatic cancer. This pathway is a collection of different mechanistic protein pat...
PLK3 signaling events, organism-specific biosystem (from Pathway Interaction Database)
PLK3 signaling events, organism-specific biosystem
PLK3 signaling events
Prostate Cancer, organism-specific biosystem (from WikiPathways)
Prostate Cancer, organism-specific biosystem
Prostate Cancer
Ubiquitin Mediated Degradation of Phosphorylated Cdc25A, organism-specific biosystem (from REACTOME)
Ubiquitin Mediated Degradation of Phosphorylated Cdc25A, organism-specific biosystemcdc25A protein is degraded by the ubiquitin-proteasome machinery in both terminally differentiating and cycling cells (Bernardi et al. 2000).
p53 pathway, organism-specific biosystem (from Pathway Interaction Database)
p53 pathway, organism-specific biosystem
p53 pathway
p53 signaling pathway, organism-specific biosystem (from KEGG)
p53 signaling pathway, organism-specific biosystemp53 activation is induced by a number of stress signals, including DNA damage, oxidative stress and activated oncogenes. The p53 protein is employed as a transcriptional activator of p53-regulated ge...
p53 signaling pathway, conserved biosystem (from KEGG)
p53 signaling pathway, conserved biosystemp53 activation is induced by a number of stress signals, including DNA damage, oxidative stress and activated oncogenes. The p53 protein is employed as a transcriptional activator of p53-regulated ge...
p53-Independent DNA Damage Response, organism-specific biosystem (from REACTOME)
p53-Independent DNA Damage Response, organism-specific biosystemIn response to DNA damage due to exposure to ultraviolet light or to ionizing radiation, Cdc25A is phosphorylated by Chk1 or Chk2. The phosphorylation of Cdc25A at ser-123, in response to DNA damage...
p53-Independent G1/S DNA damage checkpoint, organism-specific biosystem (from REACTOME)
p53-Independent G1/S DNA damage checkpoint, organism-specific biosystemThe G1 arrest induced by DNA damage has been ascribed to the transcription factor and tumor suppressor protein p53. To be effective within minutes after DNA damage, induction of the G1 block should ...

General gene information

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Markers

D22S275 (e-PCR)
- UniSTS:87591

PMC111029P1 (e-PCR)
- UniSTS:270202

PMC111029P10 (e-PCR)
- UniSTS:270203

PMC111029P12 (e-PCR)
- UniSTS:270205

PMC111029P13 (e-PCR)
- UniSTS:270206

PMC111029P14 (e-PCR)
- UniSTS:270207

PMC111029P2 (e-PCR)
- UniSTS:270208

PMC111029P3 (e-PCR)
- UniSTS:270209

PMC111029P4 (e-PCR)
- UniSTS:270210

PMC111029P5 (e-PCR)
- UniSTS:270211

PMC111029P6 (e-PCR)
- UniSTS:270212

PMC111029P7 (e-PCR)
- UniSTS:270213

PMC111029P8 (e-PCR)
- UniSTS:270214

PMC111029P9 (e-PCR)
- UniSTS:270215

PMC149355P2 (e-PCR)
- UniSTS:271033

Genotypes

See CHEK2 SNP Geneview Report
See CHEK2 SNP Genotype Report
See CHEK2 SNP Variation Viewer Report

Related pseudogene(s)

3 found Review record(s) in Gene

Homology

Homologs of the CHEK2 gene: The CHEK2 gene is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, rat, chicken, zebrafish, fruit fly, mosquito, C.elegans, M.oryzae, and N.crassa.
Map Viewer (Mouse, Rat)
OrthoDB: The Hierarchical Catalog of Eukaryotic Orthologs

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
ATP binding	IEA Inferred from Electronic Annotation more info
metal ion binding	IEA Inferred from Electronic Annotation more info
protein binding	IPI Inferred from Physical Interaction more info	PubMed
protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed
protein kinase binding	IPI Inferred from Physical Interaction more info	PubMed
protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
protein serine/threonine kinase activity	TAS Traceable Author Statement more info	PubMed
ubiquitin protein ligase binding	IPI Inferred from Physical Interaction more info

Process	Evidence Code	Pubs
DNA damage checkpoint	TAS Traceable Author Statement more info	PubMed
DNA damage induced protein phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
G2/M transition of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
cell cycle checkpoint	TAS Traceable Author Statement more info
cell division	IEA Inferred from Electronic Annotation more info
cellular protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
double-strand break repair	IMP Inferred from Mutant Phenotype more info	PubMed
intrinsic apoptotic signaling pathway in response to DNA damage	IDA Inferred from Direct Assay more info	PubMed
intrinsic apoptotic signaling pathway in response to DNA damage	IMP Inferred from Mutant Phenotype more info	PubMed
positive regulation of transcription, DNA-dependent	IDA Inferred from Direct Assay more info	PubMed
protein autophosphorylation	IDA Inferred from Direct Assay more info	PubMed
protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
protein stabilization	IDA Inferred from Direct Assay more info	PubMed
regulation of protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
regulation of transcription, DNA-dependent	IDA Inferred from Direct Assay more info	PubMed
replicative senescence	NAS Non-traceable Author Statement more info	PubMed
response to DNA damage stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
response to DNA damage stimulus	TAS Traceable Author Statement more info	PubMed
response to gamma radiation	IEA Inferred from Electronic Annotation more info
signal transduction in response to DNA damage	IDA Inferred from Direct Assay more info	PubMed
signal transduction involved in intra-S DNA damage checkpoint	IMP Inferred from Mutant Phenotype more info	PubMed
spindle assembly involved in mitosis	IMP Inferred from Mutant Phenotype more info
transcription, DNA-dependent	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
PML body	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with chromosome, telomeric region	IDA Inferred from Direct Assay more info	PubMed
nucleoplasm	TAS Traceable Author Statement more info

General protein information

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Preferred Names: serine/threonine-protein kinase Chk2

Names: serine/threonine-protein kinase Chk2; cds1 homolog; CHK2 checkpoint homolog; checkpoint-like protein CHK2

NP_001005735.1

EC 2.7.11.1

NP_001244316.1

EC 2.7.11.1

NP_009125.1

EC 2.7.11.1

NP_665861.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_008150.1 RefSeqGene

Range

5001..59092

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001005735.1 → NP_001005735.1 serine/threonine-protein kinase Chk2 isoform c

Status: REVIEWED

Description

Transcript Variant: This variant (3) contains an alternate in-frame exon compared to variant 1. The resulting isoform (c) has the same N- and C-termini but is longer compared to isoform a.

Source sequence(s)

AW778747, AY551297, BM838597

Consensus CDS

CCDS33629.1

UniProtKB/Swiss-Prot

O96017

Related

ENSP00000372023, OTTHUMP00000198970, ENST00000382580, OTTHUMT00000321016

Conserved Domains (3) summary

cd00060
Location:156 – 244
Blast Score: 124

FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...

smart00220
Location:263 – 529
Blast Score: 738

S_TKc; Serine/Threonine protein kinases, catalytic domain

cl09925
Location:261 – 554
Blast Score: 519

PKc_like; Protein Kinases, catalytic domain
NM_001257387.1 → NP_001244316.1 serine/threonine-protein kinase Chk2 isoform d

Status: REVIEWED

Description

Transcript Variant: This variant (4) contains an alternate exon compared to variant 1, that causes a frameshift. The resulting isoform (d) is shorter at the N-terminus compared to isoform a.

Source sequence(s)

AF217975

Consensus CDS

CCDS58798.1

UniProtKB/Swiss-Prot

O96017

Conserved Domains (2) summary

smart00220
Location:3 – 265
Blast Score: 729

S_TKc; Serine/Threonine protein kinases, catalytic domain

cl09925
Location:3 – 290
Blast Score: 517

PKc_like; Protein Kinases, catalytic domain
NM_007194.3 → NP_009125.1 serine/threonine-protein kinase Chk2 isoform a

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the predominant transcript and encodes isoform a.

Source sequence(s)

AF096279, AW778747, BM838597

Consensus CDS

CCDS13843.1

UniProtKB/Swiss-Prot

O96017

Related

ENSP00000329178, OTTHUMP00000198969, ENST00000328354, OTTHUMT00000321015

Conserved Domains (3) summary

cd00060
Location:93 – 201
Blast Score: 129

FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...

smart00220
Location:220 – 486
Blast Score: 738

S_TKc; Serine/Threonine protein kinases, catalytic domain

cl09925
Location:218 – 511
Blast Score: 518

PKc_like; Protein Kinases, catalytic domain
NM_145862.2 → NP_665861.1 serine/threonine-protein kinase Chk2 isoform b

Status: REVIEWED

Description

Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.

Source sequence(s)

AW778747, AY551299, BM838597

Consensus CDS

CCDS13844.1

UniProtKB/Swiss-Prot

O96017

Related

ENSP00000329012, ENST00000348295

Conserved Domains (3) summary

cd00060
Location:93 – 201
Blast Score: 131

FHA; Forkhead associated domain (FHA); found in eukaryotic and prokaryotic proteins. Putative nuclear signalling domain. FHA domains may bind phosphothreonine, phosphoserine and sometimes phosphotyrosine. In eukaryotes, many FHA domain-containing proteins ...

smart00220
Location:220 – 457
Blast Score: 573

S_TKc; Serine/Threonine protein kinases, catalytic domain

cl09925
Location:218 – 482
Blast Score: 378

PKc_like; Protein Kinases, catalytic domain

RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 104

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p10 Primary Assembly

Genomic

NC_000022.10 Reference GRCh37.p10 Primary Assembly

Range

29083731..29137822, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000154.1 Alternate HuRef

Range

12048435..12103008, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate CHM1_1.0

Genomic

NC_018933.1 Alternate CHM1_1.0

Range

13005368..13059451, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	ABBA01055237.1 (7838..44491)	None
genomic	ABBA01055238.1	None
genomic	ABBA01055239.1	None
genomic	AL117330.6 (101..25993)	None
genomic	AL121825.19 (96139..124337)	None
genomic	AMYH01012691.1 (7755..61838)	None
genomic	AY800241.1	AAV41895.1
genomic	CH471095.1	EAW59754.1
		EAW59755.1
		EAW59756.1
		EAW59757.1
mRNA	AB040105.1	BAB17231.1
mRNA	AF086904.1	AAC83693.1
mRNA	AF096279.1	AAD11784.1
mRNA	AF174135.1	AAD48504.1
mRNA	AF217975.1	AAG17218.1
mRNA	AJ131197.1	CAA10319.1
mRNA	AJ783839.1	CAH04270.1
mRNA	AK289360.1	BAF82049.1
mRNA	AK290754.1	BAF83443.1
mRNA	AW778747.1	None
mRNA	AY551295.1	AAS58456.1
mRNA	AY551296.1	AAS58457.1
mRNA	AY551297.1	AAS58458.1
mRNA	AY551298.1	AAS58459.1
mRNA	AY551299.1	AAS58460.1
mRNA	AY551300.1	AAS58461.1
mRNA	AY551301.1	AAS58462.1
mRNA	AY551302.1	AAS58463.1
mRNA	AY551303.1	AAS58464.1
mRNA	AY551304.1	AAS58465.1
mRNA	AY551305.1	AAS58466.1
mRNA	BC004207.2	AAH04207.1
mRNA	BM838597.1	None
mRNA	CR456418.1	CAG30304.1
other-genetic	CU012979.1	CAK54410.1
other-genetic	CU013267.1	CAK54709.1
other-genetic	DQ893133.2	ABM84059.1
other-genetic	DQ896413.2	ABM87412.1