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    EBP emopamil binding protein (sterol isomerase) [ Homo sapiens (human) ]

    Gene ID: 10682, updated on 10-Jun-2013
    Official Symbol
    EBPprovided by HGNC
    Official Full Name
    emopamil binding protein (sterol isomerase)provided by HGNC
    Primary source
    HGNC:3133
    See related
    HPRD:02192; MIM:300205
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    CPX; CHO2; CPXD; CDPX2
    Summary
    The protein encoded by this gene is an integral membrane protein of the endoplasmic reticulum. It is a high affinity binding protein for the antiischemic phenylalkylamine Ca2+ antagonist [3H]emopamil and the photoaffinity label [3H]azidopamil. It is similar to sigma receptors and may be a member of a superfamily of high affinity drug-binding proteins in the endoplasmic reticulum of different tissues. This protein shares structural features with bacterial and eukaryontic drug transporting proteins. It has four putative transmembrane segments and contains two conserved glutamate residues which may be involved in the transport of cationic amphiphilics. Another prominent feature of this protein is its high content of aromatic amino acid residues (>23%) in its transmembrane segments. These aromatic amino acid residues have been suggested to be involved in the drug transport by the P-glycoprotein. Mutations in this gene cause Chondrodysplasia punctata 2 (CDPX2; also known as Conradi-Hunermann syndrome). [provided by RefSeq, Jul 2008]
    Location :
    Xp11.23-p11.22
    Sequence :
    Chromosome: X; NC_000023.10 (48380164..48387104)

    Chromosome X - NC_000023.10Genomic Context describing neighboring genes Neighboring gene FtsJ RNA methyltransferase homolog 1 (E. coli) Neighboring gene porcupine homolog (Drosophila) Neighboring gene TBC1 domain family, member 25 Neighboring gene uncharacterized LOC729275 Neighboring gene RNA binding motif (RNP1, RRM) protein 3

    GeneRIFs: Gene References Into Functions What's a GeneRIF?

    Chondrodysplasia punctata 2 X-linked dominant

    Summary from GeneReviews: Chondrodysplasia Punctata 2, X-Linked Go to GeneReviews

    Disease Characteristics
    The findings in X-linked chondrodysplasia punctata 2 (CDPX2) range from fetal demise with multiple malformations and severe growth retardation to much milder manifestations, including adults with no recognizable physical abnormalities. At least 95% of liveborn individuals with CDPX2 are female with the following findings: Growth deficiency/short stature. Distinctive craniofacial appearance . Skeletal changes: stippling (chondrodysplasia punctate) on x-rays of the epiphyses of the long bones and vertebrae, the trachea and distal ends of the ribs seen in children prior to completion of normal epiphyseal ossification; rhizomelic (i.e., proximal) shortening of limbs that is often asymmetric; scoliosis. Ectodermal changes: linear or blotchy scaling ichthyosis in the newborn that usually resolves in the first months of life leaving linear or whorled atrophic patches involving hair follicles (follicular atrophoderma); coarse hair with scarring alopecia; occasional flattened or split nails; normal teeth. Ocular changes: cataracts; microphthalmia and/or microcornea. Intellect is usually normal. Rarely affected males have been identified with a phenotype that includes: hypotonia; moderate to profound developmental delay; seizures; cerebellar (primarily vermis) hypoplasia and/or Dandy-Walker variant; and agenesis of the corpus callosum.
    Diagnosis Testing
    The diagnosis of CDPX2 rests on the presence of clinical findings consistent with the diagnosis and sterol analysis of plasma, scales from skin lesions, or cultured lymphoblasts or fibroblasts showing increased concentration of 8(9)-cholestenol and 8-dehydrocholesterol. Often a confirmatory mutation is identified in EBP, the only gene in which mutations are known to cause CDPX2.
    Genetic Counseling
    CDPX2 is inherited in an X-linked manner with early gestational male lethality. Women with an EBP germline mutation have a 50% chance of transmitting the disease-causing mutation to each child: EBP mutations in sons are usually lethal; daughters will have a range of possible phenotypic expression. When the parents are clinically unaffected, the risk to the sibs of a proband appears to be low but greater than that of the general population. If the disease-causing mutation cannot be detected in the DNA extracted from the leukocytes of either parent of the proband, three possible explanations are germline mosaicism, somatic mosaicism, or a de novo mutation in the proband. Prenatal diagnosis for pregnancies at increased risk is possible if the family-specific disease-causing mutation is known.
    References

    Summary from GeneReviews: Congenital Muscular Dystrophy Overview Go to GeneReviews

    Disease Characteristics
    Congenital muscular dystrophy (CMD) is a clinically and genetically heterogeneous group of inherited muscle disorders. Muscle weakness typically presents from birth to early infancy. Affected infants typically appear "floppy" with low muscle tone and poor spontaneous movements. Affected children may present with delay or arrest of gross motor development together with joint and/or spinal rigidity. Muscle weakness may improve, worsen, or stabilize in the short term; however, with time progressive weakness and joint contractures, spinal deformities, and respiratory compromise may affect quality of life and life span. The main CMD subtypes, grouped by involved protein function and gene in which causative mutations occur, are laminin alpha-2 (merosin) deficiency (MDC1A), collagen VI-deficient CMD, the dystroglycanopathies (caused by mutations in POMT1, POMT2, FKTN, FKRP, LARGE, POMGNT1, and ISPD), SEPN1-related CMD (previously known as rigid spine syndrome, RSMD1) and LMNA-related CMD (L-CMD). Several less known CMD subtypes have been reported in a limited number of individuals. Cognitive impairment ranging from intellectual disability to mild cognitive delay, structural brain and/or eye abnormalities, and seizures are found almost exclusively in the dystroglycanopathies while white matter abnormalities without major cognitive involvement tend to be seen in the laminin alpha-2-deficient subtype.
    Diagnosis Testing
    The diagnosis of congenital muscular dystrophy relies on clinical findings, brain and muscle imaging, muscle biopsy histology (dystrophic features without the hallmarks of the structural changes seen in the congenital myopathies), muscle and/or skin immunohistochemical staining, and molecular genetic testing.
    Genetic Counseling
    The congenital muscular dystrophies are inherited in an autosomal recessive manner with the exception of collagen VI-deficient CMD which may be inherited in an autosomal recessive or an autosomal dominant manner and LMNA-related CMD (L-CMD) which is inherited in an autosomal dominant manner with all cases to date caused by de novo mutation. In autosomal recessive subtypes, each sib of an affected individual has a 25% chance of being affected, a 50% chance of being an asymptomatic carrier, and a 25% chance of being unaffected and not a carrier. Carriers are asymptomatic. Carrier testing for at-risk relatives and prenatal testing for pregnancies at increased risk are possible if the disease-causing mutations in the family are known. In autosomal dominant subtypes, the offspring of affected individuals have a 50% chance of being affected. The risk to sibs of an individual with an apparently de novo mutation is low, but not zero because of the possibility of germline mosaicism in one of the parents. Prenatal testing for pregnancies at increased risk is possible for families in which the disease-causing mutation has been identified.
    References
    Products Interactant Other Gene Complex Source Pubs Description
    Q15125 Q9BY08 EBPL    HPRD  PubMed  
    BioGRID:115921 BioGRID:106848 APP    BioGRID  PubMed Reconstituted Complex 
    BioGRID:115921 BioGRID:124173 EBPL    BioGRID  PubMed Affinity Capture-Western 
    BioGRID:115921 BioGRID:108309 ELAVL1    BioGRID  PubMed Affinity Capture-RNA 
    BioGRID:115921 BioGRID:108407 ESRRG    BioGRID  PubMed Two-hybrid 
    BioGRID:115921 BioGRID:110432 MITF    BioGRID  PubMed Two-hybrid 
    BioGRID:115921 BioGRID:113164 UBC    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:115921 BioGRID:122472 VKORC1    BioGRID  PubMed Affinity Capture-Western; Two-hybrid 

    Markers

    Homology

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    C-8 sterol isomerase activity IEA
    Inferred from Electronic Annotation
    more info
     
    cholestenol delta-isomerase activity IEA
    Inferred from Electronic Annotation
    more info
     
    drug transmembrane transporter activity TAS
    Traceable Author Statement
    more info
    PubMed 
    steroid delta-isomerase activity TAS
    Traceable Author Statement
    more info
    PubMed 
    transmembrane signaling receptor activity TAS
    Traceable Author Statement
    more info
    PubMed 
    Process Evidence Code Pubs
    cholesterol biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
     
    cholesterol biosynthetic process TAS
    Traceable Author Statement
    more info
     
    cholesterol metabolic process TAS
    Traceable Author Statement
    more info
    PubMed 
    drug transmembrane transport TAS
    Traceable Author Statement
    more info
    PubMed 
    hemopoiesis IEA
    Inferred from Electronic Annotation
    more info
     
    skeletal system development TAS
    Traceable Author Statement
    more info
    PubMed 
    small molecule metabolic process TAS
    Traceable Author Statement
    more info
     
    Component Evidence Code Pubs
    endoplasmic reticulum IDA
    Inferred from Direct Assay
    more info
     
    endoplasmic reticulum membrane TAS
    Traceable Author Statement
    more info
     
    integral to plasma membrane TAS
    Traceable Author Statement
    more info
    PubMed 
    Preferred Names
    3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase
    Names
    3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase
    sterol 8-isomerase
    D8-D7 sterol isomerase
    cholestenol Delta-isomerase
    delta(8)-Delta(7) sterol isomerase
    emopamil-binding protein (sterol isomerase)
    3-beta-hydroxysteroid-delta-8,delta-7-isomerase
    Chondrodysplasia punctata-2, X-linked dominant (Happle syndrome)
    NP_006570.1

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    Genomic

    1. NG_007452.1 RefSeqGene

      Range
      5001..11941
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_006579.2NP_006570.1  3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase

      Status: REVIEWED

      Source sequence(s)
      AU128761, BC001549, BC001572, CA488777
      Consensus CDS
      CCDS14300.1
      UniProtKB/Swiss-Prot
      Q15125
      Conserved Domains (1) summary
      pfam05241
      Location:51221
      Blast Score: 568
      EBP; Emopamil binding protein

    RefSeqs of Annotated Genomes: Homo sapiens Annotation Release 104

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh37.p10 PATCHES

    Genomic

    1. NW_004070880.1 Reference GRCh37.p10 PATCHES

      Range
      761205..768145
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Reference GRCh37.p10 Primary Assembly

    Genomic

    1. NC_000023.10 Reference GRCh37.p10 Primary Assembly

      Range
      48380164..48387104
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate HuRef

    Genomic

    1. AC_000155.1 Alternate HuRef

      Range
      46043290..46050391
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate CHM1_1.0

    Genomic

    1. NC_018934.1 Alternate CHM1_1.0

      Range
      48300202..48307143
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

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