CENPF centromere protein F, 350/400kDa (mitosin) [Homo sapiens] - Gene

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Summary

Official Symbol: CENPFprovided by HGNC
Official Full Name: centromere protein F, 350/400kDa (mitosin)provided by HGNC
Primary source: HGNC:1857
Locus tag: RP11-262H5.1
See related: Ensembl:ENSG00000117724; HPRD:02582; MIM:600236; Vega:OTTHUMG00000036955
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: CENF; hcp-1; PRO1779
Summary: This gene encodes a protein that associates with the centromere-kinetochore complex. The protein is a component of the nuclear matrix during the G2 phase of interphase. In late G2 the protein associates with the kinetochore and maintains this association through early anaphase. It localizes to the spindle midzone and the intracellular bridge in late anaphase and telophase, respectively, and is thought to be subsequently degraded. The localization of this protein suggests that it may play a role in chromosome segregation during mitotis. It is thought to form either a homodimer or heterodimer. Autoantibodies against this protein have been found in patients with cancer or graft versus host disease. [provided by RefSeq, Jul 2008]

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Genomic context

Location :: 1q32-q41

Sequence :: Chromosome: 1; NC_000001.10 (214776532..214837914)

See CENPF in Epigenomics, MapViewer

Chromosome 1 - NC_000001.10 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rab5 forms a complex with a subset of CENP-F in mitotic cells and regulates the kinetics of release of CENP-F from the nuclear envelope and its accumulation on kinetochores.
Title: Small GTPase Rab5 participates in chromosome congression and regulates localization of the centromere-associated protein CENP-F to kinetochores.
Centromere protein F and survivin are malignant behaviour markers for colorectal gastrointestinal stromal tumours.
Title: Centromere protein F and survivin are associated with high risk and a poor prognosis in colorectal gastrointestinal stromal tumours.
Data identified Cenp-F as a potential new molecular target for NBPs in tumour cells.
Title: The kinetochore protein Cenp-F is a potential novel target for zoledronic acid in breast cancer cells.
Cenp-F plays a role in organization of interphase chromatin through association and possibly regulation of DNA-PK.
Title: Involvement of Cenp-F in interphase chromatin organization possibly through association with DNA-dependent protein kinase.
Data suggest that CENP-F protein is a valuable marker of nasopharyngeal carcinoma progression, and CENP-F expression is associated with poor overall survival of patients.
Title: Prognostic significance and therapeutic implications of centromere protein F expression in human nasopharyngeal carcinoma.
the interaction between LANA and the kinetochore proteins CENP-F and Bub1 is important for KSHV genome tethering and its segregation to new daughter cells
Title: Bub1 and CENP-F can contribute to Kaposi's sarcoma-associated herpesvirus genome persistence by targeting LANA to kinetochores.
Mitosin did not predict patient survival in this series of cutaneous melanomas.
Title: Ki-67 expression is superior to mitotic count and novel proliferation markers PHH3, MCM4 and mitosin as a prognostic factor in thick cutaneous melanoma.
These results uncover a novel role of CENP-F in regulation of epigenetic modification on histone H3.
Title: Involvement of CENP-F in histone methylation.
Data show that the post-anaphase, KEN-box-dependent degradation of Cenp-F requires it to be farnesylated, a post-translational modification usually linked to membrane association.
Title: Cdc20 is required for the post-anaphase, KEN-dependent degradation of centromere protein F.
Study analyzed the distribution of PARP-1 and its interaction with CENP-B, -E, and -F during mitosis and apoptosis.
Title: Distribution of centromeric proteins and PARP-1 during mitosis and apoptosis.

Submit: New GeneRIF Correction See all (23)

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Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

Association between SNP-genotype and chronic lymphocytic leukemia outcome in a randomized chemotherapy trial.

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Products	Interactant	Other Gene	Source	Pubs	Description
NC_000001.8		FOXM1	BIND	PubMed	FoxM1 interacts with the CENP-F promoter.
P49454	BRAP2	BRAP	HPRD	PubMed
P49454	Q02224	CENPE	HPRD	PubMed
P49454	P49454	CENPF	HPRD	PubMed
P49454	Q9UQN3	CHMP2B	HPRD	PubMed
P49454	P49354	FNTA	HPRD	PubMed
P49454	P49356	FNTB	HPRD	PubMed
P49454	Q8WUM0	NUP133	HPRD	PubMed
P49454	P60880	SNAP25	HPRD	PubMed
P49454	O95985	TOP3B	HPRD	PubMed
BioGRID:107492	BioGRID:106739	ALK	BioGRID	PubMed	Affinity Capture-MS
BioGRID:107492	BioGRID:107492	CENPF	BioGRID	PubMed	Two-hybrid
BioGRID:107492	BioGRID:221186	Cep152	BioGRID	PubMed	Affinity Capture-MS
BioGRID:107492	BioGRID:123527	NDEL1	BioGRID	PubMed	Two-hybrid
BioGRID:107492	BioGRID:110929	NPM1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:107492	BioGRID:112497	SUMO2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:107492	BioGRID:112707	SVIL	BioGRID	PubMed	Two-hybrid
BioGRID:107492	BioGRID:112872	TERF1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:107492	BioGRID:112873	TERF2	BioGRID	PubMed	Affinity Capture-MS
BioGRID:107492	BioGRID:114452	TOP3B	BioGRID	PubMed	Two-hybrid
BioGRID:107492	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS; Reconstituted Complex

Function	Evidence Code	Pubs
chromatin binding	NAS Non-traceable Author Statement more info	PubMed
dynein binding	IDA Inferred from Direct Assay more info	PubMed
protein C-terminus binding	IPI Inferred from Physical Interaction more info	PubMed
protein binding	IPI Inferred from Physical Interaction more info	PubMed
protein homodimerization activity	IPI Inferred from Physical Interaction more info	PubMed
transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
DNA replication	IEA Inferred from Electronic Annotation more info
G2 phase of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
M phase of mitotic cell cycle	IDA Inferred from Direct Assay more info	PubMed
M phase of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
M phase of mitotic cell cycle	TAS Traceable Author Statement more info
cell differentiation	IEA Inferred from Electronic Annotation more info
cell division	IEA Inferred from Electronic Annotation more info
cell proliferation	NAS Non-traceable Author Statement more info	PubMed
chromosome segregation	IMP Inferred from Mutant Phenotype more info	PubMed
chromosome segregation	NAS Non-traceable Author Statement more info	PubMed
kinetochore assembly	NAS Non-traceable Author Statement more info	PubMed
metaphase plate congression	IDA Inferred from Direct Assay more info	PubMed
mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
mitotic cell cycle	TAS Traceable Author Statement more info
mitotic cell cycle spindle assembly checkpoint	NAS Non-traceable Author Statement more info	PubMed
mitotic prometaphase	TAS Traceable Author Statement more info
multicellular organismal development	IEA Inferred from Electronic Annotation more info
muscle organ development	IEA Inferred from Electronic Annotation more info
negative regulation of transcription, DNA-dependent	IDA Inferred from Direct Assay more info	PubMed
protein transport	IDA Inferred from Direct Assay more info	PubMed
protein transport	IMP Inferred from Mutant Phenotype more info	PubMed
regulation of G2/M transition of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
regulation of striated muscle tissue development	ISS Inferred from Sequence or Structural Similarity more info	PubMed
response to drug	NAS Non-traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
colocalizes_with chromatin	NAS Non-traceable Author Statement more info	PubMed
chromosome	IEA Inferred from Electronic Annotation more info
chromosome, centromeric region	IDA Inferred from Direct Assay more info	PubMed
condensed chromosome outer kinetochore	IDA Inferred from Direct Assay more info	PubMed
condensed chromosome outer kinetochore	TAS Traceable Author Statement more info	PubMed
cytoplasm	IDA Inferred from Direct Assay more info	PubMed
cytoskeleton	IEA Inferred from Electronic Annotation more info
cytosol	TAS Traceable Author Statement more info
kinetochore	IDA Inferred from Direct Assay more info	PubMed
midbody	IDA Inferred from Direct Assay more info	PubMed
nuclear envelope	IDA Inferred from Direct Assay more info	PubMed
nuclear matrix	IDA Inferred from Direct Assay more info	PubMed
nucleus	IDA Inferred from Direct Assay more info	PubMed
perinuclear region of cytoplasm	IEA Inferred from Electronic Annotation more info
spindle	IDA Inferred from Direct Assay more info	PubMed
spindle pole	IDA Inferred from Direct Assay more info	PubMed

General protein information

Preferred Names: centromere protein F

Names: centromere protein F; mitosin; AH antigen; kinetochore protein CENPF; CENP-F kinetochore protein; cell-cycle-dependent 350K nuclear protein; centromere protein F, 350/400ka (mitosin)

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NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_016343.3 → NP_057427.3 centromere protein F

Status: REVIEWED

Source sequence(s)

BG680819, BQ774943, BU155312, BU662228, CD654009, U30872

Consensus CDS

CCDS31023.1

UniProtKB/Swiss-Prot

P49454

Related

ENSP00000355922, OTTHUMP00000035028, ENST00000366955, OTTHUMT00000089749

Conserved Domains (5) summary

pfam10481
Location:1 – 288
Blast Score: 959

Cenp-F_N; Cenp-F N-terminal domain

pfam10490
Location:2965 – 3013
Blast Score: 250

Rb-bdg_C_Cenp-F; Rb-binding domain of kinetochore protein Cenp-F/LEK1

pfam10473
Location:2313 – 2452
Blast Score: 328

Cenp-F_leu_zip; Leucine-rich repeats of kinetochore protein Cenp-F/LEK1

TIGR02168
Location:1952 – 2758
Blast Score: 252

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

TIGR02169
Location:1786 – 2057
Blast Score: 120

SMC_prok_A; chromosome segregation protein SMC, primarily archaeal type

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000001.10 Reference GRCh37.p5 Primary Assembly

Range

214776532..214837914

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000133.1 Alternate HuRef

Range

185450388..185511761

Download

GenBank, FASTA, Sequence Viewer (Graphics)

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Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AL445305.11	CAH73032.1
genomic	AL445666.9	CAH71810.1
genomic	CH471100.2	EAW93350.1
genomic	HI574254.1	CBX47616.1
mRNA	AK307690.1	None
mRNA	BG680819.1	None
mRNA	BQ774943.1	None
mRNA	BU155312.1	None
mRNA	BU662228.1	None
mRNA	CD654009.1	None
mRNA	U19769.1	AAA82889.1
mRNA	U25725.1	AAA86889.1
mRNA	U30872.1	AAA82935.1
other-genetic	BC172232.1	AAI72232.1