CENPE centromere protein E, 312kDa [Homo sapiens] - Gene

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Summary

Official Symbol: CENPEprovided by HGNC
Official Full Name: centromere protein E, 312kDaprovided by HGNC
Primary source: HGNC:1856
See related: Ensembl:ENSG00000138778; HPRD:00316; MIM:117143; Vega:OTTHUMG00000160980
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: KIF10; CENP-E; PPP1R61
Summary: Centrosome-associated protein E is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. CENPE is proposed to be one of the motors responsible for mammalian chromosome movement and/or spindle elongation. [provided by RefSeq, Jul 2008]

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Genomic context

Location :: 4q24-q25

Sequence :: Chromosome: 4; NC_000004.11 (104026963..104119566, complement)

See CENPE in Epigenomics, MapViewer

Chromosome 4 - NC_000004.11 Genomic Context describing neighboring genes

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Genomic regions, transcripts, and products

Genomic Sequence

Go to nucleotideGraphics FASTA GenBank

Go to reference sequence details

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Bibliography

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that CENP-E-mediated traction forces on misaligned chromosomes are responsible for the irreversible loss of spindle-pole integrity in CLASP1/2-depleted cells.
Title: CLASPs prevent irreversible multipolarity by ensuring spindle-pole resistance to traction forces during chromosome alignment.
SKAP cooperates with CENP-E to orchestrate dynamic kinetochore-microtubule interaction for faithful chromosome segregation.
Title: CENP-E kinesin interacts with SKAP protein to orchestrate accurate chromosome segregation in mitosis.
Targeting of CENP-E and BubR1 to the kinetochores and the interaction between CENP-E and BubR1 are significantly reduced in EpoB-resistant A549 cell line, compared to A549 cells.
Title: The interaction between mitotic checkpoint proteins, CENP-E and BubR1, is diminished in epothilone B-resistant A549 cells.
MPS1 is required by cells to arrest in mitosis in response to spindle defects and kinetochore defects resulting from the loss of the kinesin-like protein.
Title: Human MPS1 kinase is required for mitotic arrest induced by the loss of CENP-E from kinetochores.
Aurora B allowed chromosome alignment in CENP-E-compromised cells; implied that by destabilizing pole proximal syntelic attachments, Aurora B cooperates with CENP-E to mediate congression of mono-oriented polar chromosomes
Title: Aurora B kinase cooperates with CENP-E to promote timely anaphase onset.
An Aurora/PP1 phosphorylation switch modulates CENP-E motor activity as a feature of chromosome congression from poles and localized PP1 delivery by CENP-E to the outer kinetochore is necessary for stable microtubule capture by those chromosomes.
Title: Aurora kinases and protein phosphatase 1 mediate chromosome congression through regulation of CENP-E.
Observational study of gene-disease association. (HuGE Navigator)
Title: A systematic gene-based screen of chr4q22-q32 identifies association of a novel susceptibility gene, DKK2, with the quantitative trait of alcohol dependence symptom counts.
CENP-E expression was reduced in human hepatocellular carcinoma
Title: Reduced expression of cenp-e in human hepatocellular carcinoma.
CENP-E integrates two critical functions that are important for accurate chromosome movement and spindle architecture: one relying directly on its motor activity, and the other involving the targeting of key microtubule regulators to kinetochores.
Title: Motor-independent targeting of CLASPs to kinetochores by CENP-E promotes microtubule turnover and poleward flux.
Studies strongly suggest that chromosome congression defects as the result of KIF18A depletion is at least in part mediated through destabilizing kinetochore CENP-E.
Title: Defects in chromosome congression and mitotic progression in KIF18A-deficient cells are partly mediated through impaired functions of CENP-E.

Submit: New GeneRIF Correction See all (22)

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Phenotypes

Review eQTL and phenotype association data in this region using PheGenI

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Products	Interactant	Other Gene	Source	Pubs	Description
Q02224	O60566	BUB1B	HPRD	PubMed
Q02224	P49454	CENPF	HPRD	PubMed
Q02224	P63208	SKP1	HPRD	PubMed
BioGRID:107491	BioGRID:107166	BUB1B	BioGRID	PubMed	Affinity Capture-Western
BioGRID:107491	BioGRID:107491	CENPE	BioGRID	PubMed	Two-hybrid
BioGRID:107491	BioGRID:114032	CUL1	BioGRID	PubMed	Two-hybrid
BioGRID:107491	BioGRID:230907	Cenpe	BioGRID	PubMed	Affinity Capture-MS
BioGRID:107491	BioGRID:109569	DNAJB1	BioGRID	PubMed	Two-hybrid
BioGRID:107491	BioGRID:108472	FANCA	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex; Two-hybrid
BioGRID:107491	BioGRID:111580	MAPK1	BioGRID	PubMed	Affinity Capture-Western; Biochemical Activity
BioGRID:107491	BioGRID:123673	NUF2	BioGRID	PubMed	Two-hybrid
BioGRID:107491	BioGRID:114517	PRC1	BioGRID	PubMed	Affinity Capture-Western; Reconstituted Complex
BioGRID:107491	BioGRID:107425	SEPT7	BioGRID	PubMed	Two-hybrid
BioGRID:107491	BioGRID:114979	TRAF4	BioGRID	PubMed	Two-hybrid
BioGRID:107491	BioGRID:113164	UBC	BioGRID	PubMed	Affinity Capture-MS; Reconstituted Complex

Function	Evidence Code	Pubs
ATP binding	IEA Inferred from Electronic Annotation more info
kinetochore binding	IDA Inferred from Direct Assay more info	PubMed
microtubule motor activity	IMP Inferred from Mutant Phenotype more info	PubMed
nucleotide binding	IEA Inferred from Electronic Annotation more info
protein binding	IPI Inferred from Physical Interaction more info	PubMed
protein kinase binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
M phase of mitotic cell cycle	TAS Traceable Author Statement more info
blood coagulation	TAS Traceable Author Statement more info
cell division	IEA Inferred from Electronic Annotation more info
establishment of protein localization	IEA Inferred from Electronic Annotation more info
kinetochore assembly	NAS Non-traceable Author Statement more info	PubMed
microtubule-based movement	TAS Traceable Author Statement more info
mitotic cell cycle	TAS Traceable Author Statement more info
mitotic cell cycle spindle assembly checkpoint	IEA Inferred from Electronic Annotation more info
mitotic chromosome movement towards spindle pole	TAS Traceable Author Statement more info	PubMed
mitotic metaphase	TAS Traceable Author Statement more info	PubMed
mitotic metaphase plate congression	TAS Traceable Author Statement more info	PubMed
mitotic prometaphase	TAS Traceable Author Statement more info
multicellular organismal development	IEA Inferred from Electronic Annotation more info
positive regulation of attachment of spindle microtubules to kinetochore	IEA Inferred from Electronic Annotation more info
positive regulation of mitotic metaphase/anaphase transition	IEA Inferred from Electronic Annotation more info
positive regulation of protein kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
regulation of developmental process	IEA Inferred from Electronic Annotation more info
regulation of mitosis	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
chromosome	IEA Inferred from Electronic Annotation more info
chromosome, centromeric region	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with condensed chromosome outer kinetochore	TAS Traceable Author Statement more info	PubMed
condensed chromosome, centromeric region	IDA Inferred from Direct Assay more info	PubMed
cytoplasm	IDA Inferred from Direct Assay more info	PubMed
cytosol	TAS Traceable Author Statement more info
kinetochore	IDA Inferred from Direct Assay more info	PubMed
microtubule	IDA Inferred from Direct Assay more info	PubMed
microtubule cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
spindle	IEA Inferred from Electronic Annotation more info

General protein information

Preferred Names: centromere-associated protein E

Names: centromere-associated protein E; kinesin family member 10; kinesin-related protein CENPE; Centromere autoantigen E (312kD); protein phosphatase 1, regulatory subunit 61

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NCBI Reference Sequences (RefSeq)

RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001813.2 → NP_001804.2 centromere-associated protein E

Status: VALIDATED

Source sequence(s)

AA210728, AA210858, AC079919, AL596934, AU100312, AW499901, BQ231878, BQ434767, BQ447425, CD644410, CN418651, Z15005

Consensus CDS

CCDS34042.1

UniProtKB/Swiss-Prot

Q02224

Related

ENSP00000265148, ENST00000265148

Conserved Domains (4) summary

cd01374
Location:6 – 329
Blast Score: 1172

KISc_CENP_E; Kinesin motor domain, CENP-E/KIP2-like subgroup, involved in chromosome movement and/or spindle elongation during mitosis. This catalytic (head) domain has ATPase activity and belongs to the larger group of P-loop NTPases. Kinesins are ...

COG1196
Location:1232 – 2071
Blast Score: 184

Smc; Chromosome segregation ATPases [Cell division and chromosome partitioning]

TIGR02168
Location:663 – 989
Blast Score: 172

SMC_prok_B; chromosome segregation protein SMC, common bacterial type

pfam02463
Location:1675 – 2574
Blast Score: 94

SMC_N; RecF/RecN/SMC N terminal domain

RefSeqs of Annotated Genomes: Build 37.3

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh37.p5 Primary Assembly

Genomic

NC_000004.11 Reference GRCh37.p5 Primary Assembly

Range

104026963..104119566, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate HuRef

Genomic

AC_000136.1 Alternate HuRef

Range

99760728..99853482, complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

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Related Sequences

Nucleotide		Protein
Heading	Accession and Version	Protein
genomic	AC079919.5 (5824..98427)	None
genomic	CH471057.1	EAX06169.1
		EAX06170.1
		EAX06171.1
genomic	HI573938.1	CBX47458.1
mRNA	AA210728.1	None
mRNA	AA210858.1	None
mRNA	AB209996.1	BAE06078.1
mRNA	AK290362.1	BAF83051.1
mRNA	AL596934.1	None
mRNA	AU100312.1	None
mRNA	AW499901.1	None
mRNA	AY429526.1	None
mRNA	BQ231878.1	None
mRNA	BQ434767.1	None
mRNA	BQ447425.1	None
mRNA	CD644410.1	None
mRNA	CN418651.1	None
mRNA	Z15005.1	CAA78727.1
other-genetic	BC156501.1	AAI56502.1