Espl1 extra spindle poles-like 1 (S. cerevisiae) [Mus musculus (house mouse)] - Gene

Summary

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Official Symbol: Espl1provided by MGI
Official Full Name: extra spindle poles-like 1 (S. cerevisiae)provided by MGI
Primary source: MGI:2146156
See related: Ensembl:ENSMUSG00000058290
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: SSE; Cerp; ESP1; PRCE; AL024103; AU045071

Genomic context

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Location :: 15 F3; 15

Sequence :: Chromosome: 15; NC_000081.6 (102296293..102324356)

See Espl1 in Epigenomics, MapViewer

Chromosome 15 - NC_000081.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence

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Go to reference sequence details

Bibliography

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GeneRIFs: Gene References Into Functions What's a GeneRIF?

With the continual loss of cohesins from chromosomes that occurs throughout the natural reproductive lifespan, tight regulation of separase in oocytes may be particularly important to maintain cohesion and prevent aneuploidy.
Title: Age-dependent susceptibility of chromosome cohesion to premature separase activation in mouse oocytes.
Separase act synergistically with loss of p53 in the initiation and progression of B- and T- cell lymphomas
Title: Separase loss of function cooperates with the loss of p53 in the initiation and progression of T- and B-cell lymphoma, leukemia and aneuploidy in mice.
Separase phospho-regulation is critical for genome stability in oogenesis.
Title: Separase phosphosite mutation leads to genome instability and primordial germ cell depletion during oogenesis.
deregulation of separase may be a significant contributor to infertility in humans.
Title: Preimplantation mouse embryos depend on inhibitory phosphorylation of separase to prevent chromosome missegregation.
Results describe a new function for the TGF-beta pathway in the regulation of APC to mediate chromatid separation during mitosis.
Title: Regulation of the anaphase-promoting complex-separase cascade by transforming growth factor-beta modulates mitotic progression in bone marrow stromal cells.
These results collectively suggest that Separase is an oncogene, whose overexpression alone in mammary epithelial cells is sufficient to induce aneuploidy and tumorigenesis in a p53 mutant background.
Title: Overexpression of Separase induces aneuploidy and mammary tumorigenesis.
inhibitory phosphorylation of separase plays a critical role in the maintenance of sister chromatid cohesion and genome stability in proliferating postmigratory primordial germ cells
Title: Inhibitory phosphorylation of separase is essential for genome stability and viability of murine embryonic germ cells.
Separase phosphorylation act redundantly to prevent sister chromatid separation.
Title: Securin and separase phosphorylation act redundantly to maintain sister chromatid cohesion in mammalian cells.
In embryonic fibroblasts, Separase depletion blocks sister chromatid separation but does not prevent other aspects of mitosis, cytokinesis, or chromosome replication.
Title: Separase: a universal trigger for sister chromatid disjunction but not chromosome cycle progression.
Proteolytic activity of separase is therefore essential for Rec8's removal from chromosome arms and for chiasma resolution but not for PBE.
Title: Resolution of chiasmata in oocytes requires separase-mediated proteolysis.

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Alleles

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Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (Floxed/Frt) (3) 1 citation
Targeted (knock-out) (2) 1 citation
Gene trapped (1)

Interactions

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Products	Interactant	Other Gene	Source	Pubs	Description
BioGRID:222968	BioGRID:107169	C1QBP	BioGRID	PubMed	Affinity Capture-MS
BioGRID:222968	BioGRID:107332	CCNB1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:222968	BioGRID:107420	CDK1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:222968	BioGRID:115052	ESPL1	BioGRID	PubMed	Affinity Capture-MS
BioGRID:222968	BioGRID:114663	PTTG1	BioGRID	PubMed	Affinity Capture-MS

Pathways from BioSystems

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Cell Cycle, organism-specific biosystem (from REACTOME)
Cell Cycle, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Cell Cycle, Mitotic, organism-specific biosystem (from REACTOME)
Cell Cycle, Mitotic, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Cell cycle, organism-specific biosystem (from KEGG)
Cell cycle, organism-specific biosystemMitotic cell cycle progression is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps known as G1 and G2 phases. Cycli...
Cell cycle, organism-specific biosystem (from WikiPathways)
Cell cycle, organism-specific biosystem(From http://en.wikipedia.org/wiki/Cell_cycle) The cell cycle is the series of events that takes place in a cell leading to its division and duplication (replication). Regulation of the cell cycle in...
Cell cycle, conserved biosystem (from KEGG)
Cell cycle, conserved biosystemMitotic cell cycle progression is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps known as G1 and G2 phases. Cycli...
Cell cycle signaling pathway, organism-specific biosystem (from LIPID MAPS)
Cell cycle signaling pathway, organism-specific biosystem
Cell cycle signaling pathway
M Phase, organism-specific biosystem (from REACTOME)
M Phase, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Mitotic Anaphase, organism-specific biosystem (from REACTOME)
Mitotic Anaphase, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Mitotic Metaphase and Anaphase, organism-specific biosystem (from REACTOME)
Mitotic Metaphase and Anaphase, organism-specific biosystemcomputationally inferred pathway (not manually curated)
Oocyte meiosis, organism-specific biosystem (from KEGG)
Oocyte meiosis, organism-specific biosystemDuring meiosis, a single round of DNA replication is followed by two rounds of chromosome segregation, called meiosis I and meiosis II. At meiosis I, homologous chromosomes recombine and then segrega...
Oocyte meiosis, conserved biosystem (from KEGG)
Oocyte meiosis, conserved biosystemDuring meiosis, a single round of DNA replication is followed by two rounds of chromosome segregation, called meiosis I and meiosis II. At meiosis I, homologous chromosomes recombine and then segrega...
Separation of Sister Chromatids, organism-specific biosystem (from REACTOME)
Separation of Sister Chromatids, organism-specific biosystemcomputationally inferred pathway (not manually curated)

General gene information

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Markers

D10Chm206 (e-PCR)
- UniSTS:518213

D10Mit157.2 (e-PCR), detects polymorphism
- UniSTS:126061

Espl1 (e-PCR), detects polymorphism
- UniSTS:546763

RH125481 (e-PCR)
- UniSTS:162300

AU045071 (e-PCR)
- UniSTS:160511

Homology

Homologs of the Espl1 gene: The Espl1 gene is conserved in human, chimpanzee, Rhesus monkey, dog, cow, rat, chicken, and zebrafish.
Map Viewer (Human, Rat)
OrthoDB: The Hierarchical Catalog of Eukaryotic Orthologs

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
cysteine-type peptidase activity	IEA Inferred from Electronic Annotation more info
hydrolase activity	IEA Inferred from Electronic Annotation more info
peptidase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
chromosome segregation	IGI Inferred from Genetic Interaction more info	PubMed
homologous chromosome segregation	IMP Inferred from Mutant Phenotype more info	PubMed
meiosis I	IMP Inferred from Mutant Phenotype more info	PubMed
meiotic spindle organization	IMP Inferred from Mutant Phenotype more info	PubMed
metabolic process	IDA Inferred from Direct Assay more info	PubMed
mitotic sister chromatid segregation	IMP Inferred from Mutant Phenotype more info	PubMed
mitotic sister chromatid segregation	ISO Inferred from Sequence Orthology more info
proteolysis	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
centrosome	ISO Inferred from Sequence Orthology more info
cytoplasm	IEA Inferred from Electronic Annotation more info
nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: separin

Names: separin; separase; Cut1/ESP1 related protein; caspase-like protein ESPL1; extra spindle poles-like 1 protein

NP_001014976.1

EC 3.4.22.49

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001014976.2 → NP_001014976.1 separin

Status: VALIDATED

Source sequence(s)

AC163291

Consensus CDS

CCDS27878.1

UniProtKB/Swiss-Prot

P60330

Related

ENSMUSP00000064465, ENSMUST00000064924

Conserved Domains (1) summary

pfam03568
Location:1712 – 2065
Blast Score: 1000

Peptidase_C50; Peptidase family C50

RefSeqs of Annotated Genomes: Mus musculus Annotation Release 103

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm38.p1 C57BL/6J

Genomic

NC_000081.6 Reference GRCm38.p1 C57BL/6J

Range

102296293..102324356

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Alternate Mm_Celera

Genomic

AC_000037.1 Alternate Mm_Celera

Range

104453712..104481413

Download

GenBank, FASTA, Sequence Viewer (Graphics)

Nucleotide		Protein	Strain
Heading	Accession and Version	Protein	Strain
genomic	AAHY01125185.1	None
genomic	AAHY01125186.1	None
genomic	AAHY01125187.1	None
genomic	AAHY01125188.1 (11979..14524)	None
genomic	AC163291.3 (129592..157655)	None
genomic	CH466550.2	EDL03991.1	mixed
		EDL03992.1	mixed
mRNA	AK076351.1	None	C57BL/6J
mRNA	AK129072.1	BAC97882.1
mRNA	AY588413.1	AAT94400.1	129/Sv
mRNA	BC082603.1	AAH82603.1	C57BL/6
mRNA	BC145844.1	AAI45845.1
mRNA	BC145846.1	AAI45847.1