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    ACOT8 acyl-CoA thioesterase 8 [ Homo sapiens ]

    Gene ID: 10005, updated on 19-May-2012
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    Summary

    Official Symbol
    ACOT8provided by HGNC
    Official Full Name
    acyl-CoA thioesterase 8provided by HGNC
    Primary source
    HGNC:15919
    Locus tag
    RP3-337O18.2
    See related
    Ensembl:ENSG00000101473; HPRD:06998; MIM:608123; Vega:OTTHUMG00000033045
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Homo sapiens
    Lineage
    Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
    Also known as
    hTE; PTE1; PTE2; PTE-1; PTE-2; HNAACTE; hACTE-III
    Summary
    The protein encoded by this gene is a peroxisomal thioesterase that appears to be involved more in the oxidation of fatty acids rather than in their formation. The encoded protein can bind to the human immunodeficiency virus-1 protein Nef, and mediate Nef-induced down-regulation of CD4 in T-cells. [provided by RefSeq, Oct 2010]
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    Genomic context

    Location :
    20q13.12
    Sequence :
    Chromosome: 20; NC_000020.10 (44470360..44486048, complement)
    See ACOT8 in Epigenomics, MapViewer

    Chromosome 20 - NC_000020.10Genomic Context describing neighboring genes Neighboring gene ubiquitin-conjugating enzyme E2C Neighboring gene troponin C type 2 (fast) Neighboring gene sorting nexin family member 21 Neighboring gene zinc finger, SWIM-type containing 3 Neighboring gene zinc finger, SWIM-type containing 1

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    Genomic regions, transcripts, and products

    Go to nucleotideGraphics FASTA GenBank

    Go to reference sequence details
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    Bibliography

    Related articles in PubMed

    1. Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level. Danielsen JM, et al. Mol Cell Proteomics, 2011 Mar. PMID 21139048.
    2. Nafamostat is hydrolysed by human liver cytosolic long-chain acyl-CoA hydrolase. Yamaori S, et al. Xenobiotica, 2007 Mar. PMID 17624024.
    3. Large-scale identification of c-MYC-associated proteins using a combined TAP/MudPIT approach. Koch HB, et al. Cell Cycle, 2007 Jan 15. PMID 17314511.
    4. Analysis of the mouse and human acyl-CoA thioesterase (ACOT) gene clusters shows that convergent, functional evolution results in a reduced number of human peroxisomal ACOTs. Hunt MC, et al. FASEB J, 2006 Sep. PMID 16940157.
    5. Biochemistry of mammalian peroxisomes revisited. Wanders RJ, et al. Annu Rev Biochem, 2006. PMID 16756494.

    See all (21) citations in PubMed

    GeneRIFs: Gene References Into Functions What's a GeneRIF?

    1. An isoform of long-chain acyl-CoA hydrolase may be responsible for the nafamostat hydrolysis in human liver cytosol.
      Title: Nafamostat is hydrolysed by human liver cytosolic long-chain acyl-CoA hydrolase.
    2. SREBP2 modulates brain palmitoyl-coa hydrolase gene transcription.
      Title: Sterol Regulatory Element-Binding Protein-2 modulates human brain acyl-CoA hydrolase gene transcription.
    3. ACOT4 and ACOT8 are responsible for the termination of beta-oxidation of dicarboxylic acids of medium-chain length with the concomitant release of the corresponding free acids
      Title: The identification of a succinyl-CoA thioesterase suggests a novel pathway for succinate production in peroxisomes.
    Submit: New GeneRIF Correction
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    Phenotypes

    Review eQTL and phenotype association data in this region using PheGenI

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    HIV-1 protein interactions

    Protein Gene Interaction Pubs
    Nef nef Point mutations in HIV-1 Nef at amino acids Asp108, Leu121, Pro122, and Asp123 abrogate the ability of Nef to bind hTE, downregulate CD4 and MHC class I, and dimerize, indicating a key role for this region of Nef in mediating most of its known functions PubMed
    nef Amino acids 108-152 mediate binding of HIV-1 Nef to the human Thioesterase II protein (hTE), an interaction that enhances hTE enzymatic activity PubMed

    Go to the HIV-1, Human Protein Interaction Database

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    Interactions

    Products Interactant Other Gene Complex Source Pubs Description
    BioGRID:115323 BioGRID:110694 MYC    BioGRID  PubMed Affinity Capture-MS 
    BioGRID:115323 BioGRID:113164 UBC    BioGRID  PubMed Affinity Capture-MS 
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    General gene information

    Markers

    Genotypes

    Homology

    Pathways from BioSystems

    • Beta-oxidation of pristanoyl-CoA, organism-specific biosystem (from REACTOME)
      Beta-oxidation of pristanoyl-CoA, organism-specific biosystemPristanoyl-CoA, generated in the peroxisome by alpha-oxidation of dietary phytanic acid, is further catabolized by three cycles of peroxisomal beta-oxidation to yield 4,8-dimethylnonanoyl-CoA, acetyl...
    • Beta-oxidation of very long chain fatty acids, organism-specific biosystem (from REACTOME)
      Beta-oxidation of very long chain fatty acids, organism-specific biosystemLinear fatty acids containing more than 18 carbons are broken down by beta-oxidation in peroxisomes to yield acetyl-CoA and medium chain-length fatty acyl CoA's such as octanoyl-CoA (Wanders and Wate...
    • Bile acid and bile salt metabolism, organism-specific biosystem (from REACTOME)
      Bile acid and bile salt metabolism, organism-specific biosystemIn a healthy adult human, about 500 mg of cholesterol is converted to bile salts daily. Newly synthesized bile salts are secreted into the bile and released into the small intestine where they emulsi...
    • Bile acid biosynthesis, cholesterol => cholate, organism-specific biosystem (from KEGG)
      Bile acid biosynthesis, cholesterol => cholate, organism-specific biosystemPathway module; Carbohydrate and lipid metabolism; Sterol biosynthesis
    • Bile acid biosynthesis, cholesterol => cholate, conserved biosystem (from KEGG)
      Bile acid biosynthesis, cholesterol => cholate, conserved biosystemPathway module; Carbohydrate and lipid metabolism; Sterol biosynthesis
    • Metabolic pathways, organism-specific biosystem (from KEGG)
      Metabolic pathways, organism-specific biosystem
      Metabolic pathways
    • Metabolism, organism-specific biosystem (from REACTOME)
      Metabolism, organism-specific biosystemMetabolic processes in human cells generate energy through the oxidation of molecules consumed in the diet and mediate the synthesis of diverse essential molecules not taken in the diet as well as th...
    • Metabolism of lipids and lipoproteins, organism-specific biosystem (from REACTOME)
      Metabolism of lipids and lipoproteins, organism-specific biosystemLipids are hydrophobic but otherwise chemically diverse molecules that play a wide variety of roles in human biology. They include ketone bodies, fatty acids, triacylglycerols, phospholipids and sphi...
    • Peroxisomal lipid metabolism, organism-specific biosystem (from REACTOME)
      Peroxisomal lipid metabolism, organism-specific biosystemIn humans, the catabolism of phytanate, pristanate, and very long chain fatty acids as well as the first four steps of the biosynthesis of plasmalogens are catalyzed by peroxisomal enzymes. Defects i...
    • Peroxisome, organism-specific biosystem (from KEGG)
      Peroxisome, organism-specific biosystemPeroxisomes are essential organelles that play a key role in redox signalling and lipid homeostasis. They contribute to many crucial metabolic processes such as fatty acid oxidation, biosynthesis of ...
    • Peroxisome, conserved biosystem (from KEGG)
      Peroxisome, conserved biosystemPeroxisomes are essential organelles that play a key role in redox signalling and lipid homeostasis. They contribute to many crucial metabolic processes such as fatty acid oxidation, biosynthesis of ...
    • Primary bile acid biosynthesis, organism-specific biosystem (from KEGG)
      Primary bile acid biosynthesis, organism-specific biosystemBile acids are steroid carboxylic acids derived from cholesterol in vertebrates. The primary bile acids, cholic acid and chenodeoxycholic acid, are synthesized in the liver and conjugated with taurin...
    • Primary bile acid biosynthesis, conserved biosystem (from KEGG)
      Primary bile acid biosynthesis, conserved biosystemBile acids are steroid carboxylic acids derived from cholesterol in vertebrates. The primary bile acids, cholic acid and chenodeoxycholic acid, are synthesized in the liver and conjugated with taurin...
    • Synthesis of bile acids and bile salts, organism-specific biosystem (from REACTOME)
      Synthesis of bile acids and bile salts, organism-specific biosystemIn a healthy adult human, about 500 mg of cholesterol is converted to bile salts daily (Russell 2003). The major pathway for bile salt synthesis in the liver begins with the conversion of cholesterol...
    • Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol, organism-specific biosystem (from REACTOME)
      Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol, organism-specific biosystemIn the liver, synthesis of bile acids and bile salts is initiated with the conversion of cholesterol to 7alpha-hydroxycholesterol and of 7alpha-hydroxycholesterol to 4-cholesten-7alpha-ol-3-one. The ...
    • acyl-CoA hydrolysis, organism-specific biosystem (from BIOCYC)
      acyl-CoA hydrolysis, organism-specific biosystem
      acyl-CoA hydrolysis

    Gene Ontology Provided by GOA

    Function Evidence Code Pubs
    acyl-CoA hydrolase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    carboxylesterase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    choloyl-CoA hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    hydrolase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acyl-CoA metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    bile acid biosynthetic process TAS
    Traceable Author Statement
    more info
    		  
    bile acid metabolic process TAS
    Traceable Author Statement
    more info
    		  
    cellular lipid metabolic process TAS
    Traceable Author Statement
    more info
    		  
    fatty acid beta-oxidation using acyl-CoA oxidase TAS
    Traceable Author Statement
    more info
    		  
    interspecies interaction between organisms IEA
    Inferred from Electronic Annotation
    more info
    		  
    peroxisome organization IEA
    Inferred from Electronic Annotation
    more info
    		  
    small molecule metabolic process TAS
    Traceable Author Statement
    more info
    		  
    Component Evidence Code Pubs
    mitochondrion IEA
    Inferred from Electronic Annotation
    more info
    		  
    peroxisomal matrix IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    peroxisomal matrix TAS
    Traceable Author Statement
    more info
    		  
    peroxisome IEA
    Inferred from Electronic Annotation
    more info
    		  
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    General protein information

    Preferred Names
    acyl-coenzyme A thioesterase 8
    Names
    acyl-coenzyme A thioesterase 8
    thioesterase II
    thioesterase III
    choloyl-CoA hydrolase
    palmitoyl-CoA hydrolase
    choloyl-coenzyme A thioesterase
    long-chain fatty-acyl-CoA hydrolase
    peroxisomal acyl-CoA thioesterase 1
    HIV-Nef associated acyl-CoA thioesterase
    peroxisomal long-chain acyl-CoA thioesterase 1
    peroxisomal acyl-coenzyme A thioester hydrolase 1
    NP_005460.2
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    NCBI Reference Sequences (RefSeq)

    RefSeqs maintained independently of Annotated Genomes

    These reference sequences exist independently of genome builds. Explain

    These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

    mRNA and Protein(s)

    1. NM_005469.3 → NP_005460.2  acyl-coenzyme A thioesterase 8

      Status: REVIEWED

      Source sequence(s)
      BC117157, BM986979, DB470803, X86032
      Consensus CDS
      CCDS13378.1
      UniProtKB/Swiss-Prot
      O14734
      Related
      ENSP00000217455, OTTHUMP00000031675, ENST00000217455, OTTHUMT00000080338
      Conserved Domains (3) summary
      cd03444
      Location:205 – 308
      Blast Score: 350
      Thioesterase_II_repeat1; Thioesterase II (TEII) is thought to regenerate misprimed nonribosomal peptide synthetases (NRPSs) as well as modular polyketide synthases (PKSs) by hydrolyzing acetyl groups bound to the peptidyl carrier protein (PCP) and acyl carrier protein (ACP) ...
      cd03445
      Location:40 – 130
      Blast Score: 322
      Thioesterase_II_repeat2; Thioesterase II (TEII) is thought to regenerate misprimed nonribosomal peptide synthetases (NRPSs) as well as modular polyketide synthases (PKSs) by hydrolyzing acetyl groups bound to the peptidyl carrier protein (PCP) and acyl carrier protein (ACP) ...
      TIGR00189
      Location:35 – 309
      Blast Score: 963
      tesB; acyl-CoA thioesterase II

    RefSeqs of Annotated Genomes: Build 37.3

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference GRCh37.p5 Primary Assembly

    Genomic

    1. NC_000020.10 Reference GRCh37.p5 Primary Assembly

      Range
      44470360..44486048, complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Alternate HuRef

    Genomic

    1. AC_000152.1 Alternate HuRef

      Range
      41212129..41227770, complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    Suppressed Reference Sequence(s)

    The following Reference Sequences have been suppressed. Explain

    1. NM_183385.1: Suppressed sequence

      Description
      NM_183385.1: This RefSeq was temporary suppressed because insufficient evidence exists for the exon combination of this transcript variant, and because it is nonsense-mediated decay (NMD) candidate.

    2. NM_183386.1: Suppressed sequence

      Description
      NM_183386.1: This RefSeq was permanently suppressed because currently there is insufficient support for the transcript and the protein.
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    Related Sequences

    Nucleotide Protein
    Heading Accession and Version
    genomic AL008726.3 CAA15502.1
      CAC36012.1
      CAC36013.1
    genomic AL008726.3 CAA15502.1
      CAC36012.1
      CAC36013.1
    genomic CH471077.2 EAW75794.1
      EAW75795.1
      EAW75796.1
      EAW75797.1
      EAW75798.1
    mRNA AF014404.1 AAB71665.1
    mRNA AF124264.1 AAD27616.1
    mRNA AK056668.1 BAG51780.1
    mRNA AK292494.1 BAF85183.1
    mRNA AK296973.1 BAG59516.1
    mRNA AK298783.1 BAG60922.1
    mRNA BC117155.1 AAI17156.1
    mRNA BC117157.1 AAI17158.1
    mRNA BI911038.1 None
    mRNA BM986979.1 None
    mRNA CR541771.1 CAG46570.1
    mRNA CR541778.1 CAG46577.1
    mRNA DB470803.3 None
    mRNA X86032.1 CAA60024.1
    other-genetic HQ258192.1 ADR82946.1
    Protein Accession Links
    GenPept Link UniProtKB Link
    O14734.1 GenPept UniProtKB/Swiss-Prot:O14734
    Q6FHI2 GenPept UniProtKB/TrEMBL:Q6FHI2
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    Additional Links

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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